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The map label for this gene is budB [H]

Identifier: 186476838

GI number: 186476838

Start: 2339073

End: 2340725

Strand: Reverse

Name: budB [H]

Synonym: Bphy_2084

Alternate gene names: 186476838

Gene position: 2340725-2339073 (Counterclockwise)

Preceding gene: 186476839

Following gene: 186476837

Centisome position: 67.28

GC content: 62.98

Gene sequence:

>1653_bases
ATGAAAGCATCGGATCTGTTCGTCAAATCGCTGGAGGCTGAAGGCGTCGAGTACGTGTTCGGCATTCCCGGCGAAGAAAA
TCTCGATCTCCTCGAATCGCTGCGCCGCTCCCGCATCAAGCTCGTGCTGACGCGCCATGAACAGGCGGCCGGTTTCATGG
CCGCGACCTATGGCCGTCTGACGGGGCGAACGGGGGTGTGTCTCGCGACACTCGGGCCGGGCGCCACTAACTTCGTCACG
GCCGCCGCGTATGCGCAGCTGGGCGGCATGCCGATGCTGATGGTCACGGGCCAGAAGCCGATCAAGTCGAGCAAGCAGGG
CCATTTCCAGATCGTCGACGTGGTGCGGATGATGGAGCCGCTCACCAAATACACGCGCCAGATCGTGTCGATCGGCAATA
TTCCCGCGTCGGTGCGCGAGGCGTTCCGGCAGGCCGAGGAAGAGCGACCGGGCGCCACGCATCTCGAGCTGCCCGAGGAC
GTCGCGCACGAGGAGGGCGACGGCAAGCCGATTCCGAAGAGCTACAGCCGCCGGCCCGTCGCAGAAGAAAAAGCTGTCGC
ACACGCGGTGAAGGCGATCGTCGAGGCGAAGCATCCGTTGCTGATGATCGGCGCGGGCGGCAACCGCAAGACGACGCGCA
AGATGCTGCGCGAATTCGTCGACCAGATCGGCATCCCGTTTTTCACCACGCAAATGGGCAAGGGCGTGATCGACGAGTCG
CATCCGCTGTGGCTCGGCAATGCGACGCTGTCGGACGGCGACTTCGTGCACCGCGCGATCGATCACGCCGACTGCATCAT
CAACGTCGGTCACGACGTGATCGAAAAGCCGCCGTTCTTCATGCGCGGCGCGGATGCCAACGAGAAGACGGTGATCCACG
TGAACTTTCTCGGCGCGGAAGTGGACACTGTTTATTTCCCGCAGATCGAAGTGGTCGGCGATATCGCGAACGCAGTGTGG
CAGATGAAGGAAGCGCTGCGGGGCACGGGCGAGCATTGGGACTTCGCGCGCTTCAAGGAGATCAAGGCGCATTTCGAAGC
GCATCTGGCGAAGGGCCAGCACGACGACCGCTTCCCGATGTACCCGGTGCGGGTCGTCAACGACGTGTACGAGACGATGC
CCGTCGACGGCATCATCTGTCTCGACAACGGCATGTACAAGATCTGGTTCGCACGCTACTACCGCGCGCACGAACCGAAC
TCTCTGCTGCTCGACAATGCGCTCGCGTCGATGGGCGCCGGCCTGCCGTCGGCGATCGCGACCAAGATCGTGCATCCTGA
GCGAAAGGTGATGGCCGTGTGCGGCGACGGCGGGTTCATGATGAATTCGCAGGAACTGGAGACGGCCGTGCGGCTGAAGC
TCGATCTCGTCGTGCTGATCCTGCGCGACGACGCGTTCGGCATGATCCGCTGGAAGCAGGAAAACATGAATTTCCCCGAC
TACGGAATGATGCTCAGCAACCCCGATTTCGTCGATTACGCGACGAGCTATGGCGCGTTGGGGCACCGCGTCGGGGCGGC
GGAAGAGCTGGCGCCGCTCGTGCGCGCGTGCTTCGCGACGCCGGGCGTGCACGTGATCGACGTGCCCATCGATTACTCCG
ACAACGAGCGCGTGCTGAATCGCGAGATCAAGCGCCTGTCCGCGCAGCTTTGA

Upstream 100 bases:

>100_bases
GCATAGGCGCGGCTGTCATCGTTTTCGATCCTCCTTGTCCGCGCGCCGTCGAACGAGGTGTCACGGCCCGGTCTGCGTTC
GTTCGTCAAGCGAGACTTCC

Downstream 100 bases:

>100_bases
GGTTTCAGTCCGGCGCGTGACGGTGGGGCGGCGGTGGAACCGCCCTTGCTGCGCGTCGCTGATCGATCAAGGAGAACGAG
TCATGTTGCAGAAGAGTTAT

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MKASDLFVKSLEAEGVEYVFGIPGEENLDLLESLRRSRIKLVLTRHEQAAGFMAATYGRLTGRTGVCLATLGPGATNFVT
AAAYAQLGGMPMLMVTGQKPIKSSKQGHFQIVDVVRMMEPLTKYTRQIVSIGNIPASVREAFRQAEEERPGATHLELPED
VAHEEGDGKPIPKSYSRRPVAEEKAVAHAVKAIVEAKHPLLMIGAGGNRKTTRKMLREFVDQIGIPFFTTQMGKGVIDES
HPLWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRGADANEKTVIHVNFLGAEVDTVYFPQIEVVGDIANAVW
QMKEALRGTGEHWDFARFKEIKAHFEAHLAKGQHDDRFPMYPVRVVNDVYETMPVDGIICLDNGMYKIWFARYYRAHEPN
SLLLDNALASMGAGLPSAIATKIVHPERKVMAVCGDGGFMMNSQELETAVRLKLDLVVLILRDDAFGMIRWKQENMNFPD
YGMMLSNPDFVDYATSYGALGHRVGAAEELAPLVRACFATPGVHVIDVPIDYSDNERVLNREIKRLSAQL

Sequences:

>Translated_550_residues
MKASDLFVKSLEAEGVEYVFGIPGEENLDLLESLRRSRIKLVLTRHEQAAGFMAATYGRLTGRTGVCLATLGPGATNFVT
AAAYAQLGGMPMLMVTGQKPIKSSKQGHFQIVDVVRMMEPLTKYTRQIVSIGNIPASVREAFRQAEEERPGATHLELPED
VAHEEGDGKPIPKSYSRRPVAEEKAVAHAVKAIVEAKHPLLMIGAGGNRKTTRKMLREFVDQIGIPFFTTQMGKGVIDES
HPLWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRGADANEKTVIHVNFLGAEVDTVYFPQIEVVGDIANAVW
QMKEALRGTGEHWDFARFKEIKAHFEAHLAKGQHDDRFPMYPVRVVNDVYETMPVDGIICLDNGMYKIWFARYYRAHEPN
SLLLDNALASMGAGLPSAIATKIVHPERKVMAVCGDGGFMMNSQELETAVRLKLDLVVLILRDDAFGMIRWKQENMNFPD
YGMMLSNPDFVDYATSYGALGHRVGAAEELAPLVRACFATPGVHVIDVPIDYSDNERVLNREIKRLSAQL
>Mature_550_residues
MKASDLFVKSLEAEGVEYVFGIPGEENLDLLESLRRSRIKLVLTRHEQAAGFMAATYGRLTGRTGVCLATLGPGATNFVT
AAAYAQLGGMPMLMVTGQKPIKSSKQGHFQIVDVVRMMEPLTKYTRQIVSIGNIPASVREAFRQAEEERPGATHLELPED
VAHEEGDGKPIPKSYSRRPVAEEKAVAHAVKAIVEAKHPLLMIGAGGNRKTTRKMLREFVDQIGIPFFTTQMGKGVIDES
HPLWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRGADANEKTVIHVNFLGAEVDTVYFPQIEVVGDIANAVW
QMKEALRGTGEHWDFARFKEIKAHFEAHLAKGQHDDRFPMYPVRVVNDVYETMPVDGIICLDNGMYKIWFARYYRAHEPN
SLLLDNALASMGAGLPSAIATKIVHPERKVMAVCGDGGFMMNSQELETAVRLKLDLVVLILRDDAFGMIRWKQENMNFPD
YGMMLSNPDFVDYATSYGALGHRVGAAEELAPLVRACFATPGVHVIDVPIDYSDNERVLNREIKRLSAQL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=547, Percent_Identity=27.0566727605119, Blast_Score=179, Evalue=8e-45,
Organism=Homo sapiens, GI21361361, Length=539, Percent_Identity=26.7161410018553, Blast_Score=164, Evalue=3e-40,
Organism=Escherichia coli, GI1790104, Length=474, Percent_Identity=30.168776371308, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI87081685, Length=527, Percent_Identity=28.4629981024668, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1786717, Length=540, Percent_Identity=25.1851851851852, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI1787096, Length=532, Percent_Identity=24.812030075188, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788716, Length=542, Percent_Identity=24.3542435424354, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17542570, Length=546, Percent_Identity=24.9084249084249, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=488, Percent_Identity=24.3852459016393, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=488, Percent_Identity=24.3852459016393, Blast_Score=129, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=556, Percent_Identity=28.7769784172662, Blast_Score=206, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6320816, Length=482, Percent_Identity=25.103734439834, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321524, Length=481, Percent_Identity=22.8690228690229, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=269, Percent_Identity=27.8810408921933, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=277, Percent_Identity=27.7978339350181, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320123, Length=288, Percent_Identity=24.6527777777778, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922626, Length=481, Percent_Identity=26.8191268191268, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61002; Mature: 61002

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASDLFVKSLEAEGVEYVFGIPGEENLDLLESLRRSRIKLVLTRHEQAAGFMAATYGRL
CCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHHH
TGRTGVCLATLGPGATNFVTAAAYAQLGGMPMLMVTGQKPIKSSKQGHFQIVDVVRMMEP
CCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
LTKYTRQIVSIGNIPASVREAFRQAEEERPGATHLELPEDVAHEEGDGKPIPKSYSRRPV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
AEEKAVAHAVKAIVEAKHPLLMIGAGGNRKTTRKMLREFVDQIGIPFFTTQMGKGVIDES
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCC
HPLWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRGADANEKTVIHVNFLGAE
CCEEECCCCCCCCHHHHHHHHHHHHEEECCHHHHCCCCCEEECCCCCCCEEEEEEEECCC
VDTVYFPQIEVVGDIANAVWQMKEALRGTGEHWDFARFKEIKAHFEAHLAKGQHDDRFPM
CCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
YPVRVVNDVYETMPVDGIICLDNGMYKIWFARYYRAHEPNSLLLDNALASMGAGLPSAIA
CHHHHHHHHHHHCCCCCEEEECCCEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHH
TKIVHPERKVMAVCGDGGFMMNSQELETAVRLKLDLVVLILRDDAFGMIRWKQENMNFPD
HHHCCCCCEEEEEECCCCEEECHHHHHHHHHEEEEEEEEEEECCCCEEEEEECCCCCCCC
YGMMLSNPDFVDYATSYGALGHRVGAAEELAPLVRACFATPGVHVIDVPIDYSDNERVLN
CCCEECCCCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHH
REIKRLSAQL
HHHHHHHCCC
>Mature Secondary Structure
MKASDLFVKSLEAEGVEYVFGIPGEENLDLLESLRRSRIKLVLTRHEQAAGFMAATYGRL
CCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHHH
TGRTGVCLATLGPGATNFVTAAAYAQLGGMPMLMVTGQKPIKSSKQGHFQIVDVVRMMEP
CCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
LTKYTRQIVSIGNIPASVREAFRQAEEERPGATHLELPEDVAHEEGDGKPIPKSYSRRPV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
AEEKAVAHAVKAIVEAKHPLLMIGAGGNRKTTRKMLREFVDQIGIPFFTTQMGKGVIDES
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCC
HPLWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRGADANEKTVIHVNFLGAE
CCEEECCCCCCCCHHHHHHHHHHHHEEECCHHHHCCCCCEEECCCCCCCEEEEEEEECCC
VDTVYFPQIEVVGDIANAVWQMKEALRGTGEHWDFARFKEIKAHFEAHLAKGQHDDRFPM
CCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
YPVRVVNDVYETMPVDGIICLDNGMYKIWFARYYRAHEPNSLLLDNALASMGAGLPSAIA
CHHHHHHHHHHHCCCCCEEEECCCEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHH
TKIVHPERKVMAVCGDGGFMMNSQELETAVRLKLDLVVLILRDDAFGMIRWKQENMNFPD
HHHCCCCCEEEEEECCCCEEECHHHHHHHHHEEEEEEEEEEECCCCEEEEEECCCCCCCC
YGMMLSNPDFVDYATSYGALGHRVGAAEELAPLVRACFATPGVHVIDVPIDYSDNERVLN
CCCEECCCCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHH
REIKRLSAQL
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644303 [H]