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The map label for this gene is ilvI [H]

Identifier: 186476777

GI number: 186476777

Start: 2273638

End: 2275395

Strand: Reverse

Name: ilvI [H]

Synonym: Bphy_2022

Alternate gene names: 186476777

Gene position: 2275395-2273638 (Counterclockwise)

Preceding gene: 186476789

Following gene: 186476776

Centisome position: 65.4

GC content: 62.17

Gene sequence:

>1758_bases
ATGCCCAGCGCGGAATTCTCCACGTCGGATACCACACCACACCACGAAGCCGACTCTATCGGCGCCACCGTGCTCATGAA
GGCACTGGCCGACGAAGAAGTCGAATTCGTCTGGGGCTATCCCGGCGGCTCGGTACTCTACATCTACGACGAGCTTTACA
AGCAGGACAAATTCCAGCACGTCCTCGTGCGCCACGAGCAGGCCGCAGTCCACGCCGCTGACGCCTACGCACGCTCGACC
GGCAAGGTCGGCGTCTGCCTCGTGACCTCCGGGCCCGGCGTCACCAATGCCGTGACGGGCATCGCGACGGCCTATATGGA
TTCGATCCCTATGGTCGTCATCAGCGGCCAGGTGCCGACTGCCGCGATCGGCCAGGATGCGTTCCAGGAGTGCGACACGG
TCGGCATCACGCGTCCCTGCGTGAAACACAACTTCCTCGTGAAGGACGTGCGCGATCTTGCCGCCACCGTCAAGAAAGCT
TTCTACATCGCCCGAACCGGCCGTCCCGGCCCGGTGCTGATCGACATTCCGAAAGACGTGTCGAAGGCGCCGTGCCAGTA
CGAACCGATCAAGAGCGTGTCGCTGCGCTCGTACAACCCGGTCACGAAGGGCCATTCGGGCCAGATCCGCAAGGCCGTGG
CGCTGCTGCTGTCCGCCAGGCGCCCCTACATCTATACGGGCGGCGGCATCATCCTCGCGGATGCGTCGCGCGAACTGAAC
CAGTTCGCCGATCTGCTCGGCTATCCCGTCACGAACACGCTGATGGGACTGGGCGGCTACCGCGCGAGCGACAAGAAATT
TCTCGGCATGCTCGGCATGCACGGCACGTACGAAGCCAACATGGCGATGCAGCACTGCGACGTGCTGATCGCGATCGGCG
CGCGTTTCGACGACCGCGTGATCGGCGATCCGAGCCACTTCGCATCGCGCCCGCGCAAGATCATCCACATCGACATCGAC
CCGTCGTCGATTTCGAAGCGCGTGAAGGTCGATATCCCGATCGTCGGCGACGTGAAGGAAGTGCTGAAGGAACTGATCGA
GCAATTGCAGACGGCCGAGCATGGCCCCGACACGGCGGCGCTCGCCGACTGGTGGAAGGACATCGAAGCCTGGCGTGCGA
AAGACTGCCTGAAGTTCGACCGCGAGAGCGACATCATCAAGCCGCAATACGTGGTGGAAAAGGCGTGGGAACTGACGGAC
GGCAATGCCTTCGTGTGTTCCGACGTCGGTCAGCACCAGATGTGGGCGGCACAGTTCTATCGCTTCAACAAGCCGCGTCG
CTGGATCAATTCCGGTGGTCTCGGCACGATGGGCTTCGGCCTGCCGGCGGCGATGGGCGTGAAGATGGCGCACCCGGACG
ACGACGTGCTCTGCATCACGGGCGAAGGCTCGATCCAGATGTGCATCCAGGAGCTGTCCACCTGCAAGCAGTATGAGACG
CCCGTCAAGATCATCTCGCTCAACAACCGCTATCTGGGCATGGTGCGCCAGTGGCAGCAGATCGAATACAGCAAGCGCTA
TTCGCATTCCTACATGGATGCGCTGCCCGATTTCGTGAAGCTCGCCGAAGCGTACGGCCACGTCGGCATGCGGATCGAAA
AGACCGCCGACGTGGAACCGGCGCTGAAGGAAGCGCTGCGCCTGAAAGACCGCACGGTGTTTCTCGACTTCCAGACCGAT
CCGACCGAAAACGTCTGGCCGATGGTTCAGGCCGGCAAGGGCATCACGGAGATGCTCCTCGGGTCGGAAGATCTATAA

Upstream 100 bases:

>100_bases
TTCCTGTCAAACTGGACGCGCCGCTTGAACCCGAGCCACAAGCCCGGCAGAGAGCACCCGATCAATTTTTTGCCGAAAAT
TCGAAAGGTGAACGATGAAT

Downstream 100 bases:

>100_bases
CGAGCGCTTTCCGGCGCCGGCTACGTCGTGCGCGTCTCCACGAAAGACGGGAGCGGACGTAGCGGCGCGGACAGCACGCA
TCGCATACATCGACATCTGG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MPSAEFSTSDTTPHHEADSIGATVLMKALADEEVEFVWGYPGGSVLYIYDELYKQDKFQHVLVRHEQAAVHAADAYARST
GKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVKKA
FYIARTGRPGPVLIDIPKDVSKAPCQYEPIKSVSLRSYNPVTKGHSGQIRKAVALLLSARRPYIYTGGGIILADASRELN
QFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPSHFASRPRKIIHIDID
PSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTAALADWWKDIEAWRAKDCLKFDRESDIIKPQYVVEKAWELTD
GNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQYET
PVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTADVEPALKEALRLKDRTVFLDFQTD
PTENVWPMVQAGKGITEMLLGSEDL

Sequences:

>Translated_585_residues
MPSAEFSTSDTTPHHEADSIGATVLMKALADEEVEFVWGYPGGSVLYIYDELYKQDKFQHVLVRHEQAAVHAADAYARST
GKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVKKA
FYIARTGRPGPVLIDIPKDVSKAPCQYEPIKSVSLRSYNPVTKGHSGQIRKAVALLLSARRPYIYTGGGIILADASRELN
QFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPSHFASRPRKIIHIDID
PSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTAALADWWKDIEAWRAKDCLKFDRESDIIKPQYVVEKAWELTD
GNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQYET
PVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTADVEPALKEALRLKDRTVFLDFQTD
PTENVWPMVQAGKGITEMLLGSEDL
>Mature_584_residues
PSAEFSTSDTTPHHEADSIGATVLMKALADEEVEFVWGYPGGSVLYIYDELYKQDKFQHVLVRHEQAAVHAADAYARSTG
KVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVKKAF
YIARTGRPGPVLIDIPKDVSKAPCQYEPIKSVSLRSYNPVTKGHSGQIRKAVALLLSARRPYIYTGGGIILADASRELNQ
FADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPSHFASRPRKIIHIDIDP
SSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTAALADWWKDIEAWRAKDCLKFDRESDIIKPQYVVEKAWELTDG
NAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQYETP
VKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTADVEPALKEALRLKDRTVFLDFQTDP
TENVWPMVQAGKGITEMLLGSEDL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=583, Percent_Identity=24.6998284734134, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI21361361, Length=523, Percent_Identity=27.3422562141491, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI87081685, Length=571, Percent_Identity=57.2679509632224, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=41.25, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=34.0823970037453, Blast_Score=308, Evalue=5e-85,
Organism=Escherichia coli, GI1787096, Length=566, Percent_Identity=27.9151943462898, Blast_Score=199, Evalue=6e-52,
Organism=Escherichia coli, GI1788716, Length=554, Percent_Identity=23.826714801444, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=553, Percent_Identity=25.8589511754069, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=553, Percent_Identity=25.8589511754069, Blast_Score=155, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=535, Percent_Identity=26.7289719626168, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=581, Percent_Identity=43.2013769363167, Blast_Score=457, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320816, Length=475, Percent_Identity=24, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=524, Percent_Identity=22.5190839694656, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922626, Length=565, Percent_Identity=25.8407079646018, Blast_Score=179, Evalue=5e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64688; Mature: 64557

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSAEFSTSDTTPHHEADSIGATVLMKALADEEVEFVWGYPGGSVLYIYDELYKQDKFQH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEHHHHHHHHHHH
VLVRHEQAAVHAADAYARSTGKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPT
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
AAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVKKAFYIARTGRPGPVLIDIPKDV
HHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCC
SKAPCQYEPIKSVSLRSYNPVTKGHSGQIRKAVALLLSARRPYIYTGGGIILADASRELN
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHH
QFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRV
HHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCCCC
IGDPSHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTAA
CCCCHHHHCCCCEEEEEEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHH
LADWWKDIEAWRAKDCLKFDRESDIIKPQYVVEKAWELTDGNAFVCSDVGQHQMWAAQFY
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHH
RFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQYET
HCCCCHHHHCCCCCCCCCCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCC
PVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTADVEP
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCH
ALKEALRLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLLGSEDL
HHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC
>Mature Secondary Structure 
PSAEFSTSDTTPHHEADSIGATVLMKALADEEVEFVWGYPGGSVLYIYDELYKQDKFQH
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEHHHHHHHHHHH
VLVRHEQAAVHAADAYARSTGKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPT
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
AAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVKKAFYIARTGRPGPVLIDIPKDV
HHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCC
SKAPCQYEPIKSVSLRSYNPVTKGHSGQIRKAVALLLSARRPYIYTGGGIILADASRELN
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHH
QFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRV
HHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCCCC
IGDPSHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTAA
CCCCHHHHCCCCEEEEEEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHH
LADWWKDIEAWRAKDCLKFDRESDIIKPQYVVEKAWELTDGNAFVCSDVGQHQMWAAQFY
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHH
RFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQYET
HCCCCHHHHCCCCCCCCCCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCC
PVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTADVEP
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCH
ALKEALRLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLLGSEDL
HHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]