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The map label for this gene is pphA [H]

Identifier: 186476654

GI number: 186476654

Start: 2152898

End: 2153632

Strand: Reverse

Name: pphA [H]

Synonym: Bphy_1899

Alternate gene names: 186476654

Gene position: 2153632-2152898 (Counterclockwise)

Preceding gene: 186476655

Following gene: 186476653

Centisome position: 61.9

GC content: 63.4

Gene sequence:

>735_bases
TTGATCACGAAATACGTGCGGCGCTTTGCGCGCAACACCGTCGGCATCGACTATGCCGTCGGCGACATCCACGGCTGCTT
CGCCGCGCTCGAGATCGAGCTCGCGAAGATCCGTTTCGATGAGAGGGTCGACCGTCTCTTTTCCGTCGGCGATTTGGTCG
ACCGTGGCCCGCAGTCGGATACCGCCGTCGAGTGGATCAGCAAACCGTGGTTCCACGCCGTTCAGGGCAATCACGAAGAC
ATGGCTATCCGCTACGTCGAGCCCGGCAATCGGGACCGTGACCATTACATGATGACCGGCGGCGCATGGCTGATCGAGAA
GCCGCGCTATGCGCAGGTCATGATCGCCGACGCTCTCGCCGAACTGCCGTACGCGATCGAGGTGGAGACGGATGGCGGCC
TCGTCGGGATCGTGCACGCCGACGTCCACGGCAAAAGCTGGTCTGACATGGTCCAGGCGTTCGCCTGCGCCGACAGCGGC
ACGAAGCTGCGACGTATCACGAACAGCGTGCTGTGGAACCGCGACCGCATTCAGGACGAAGACCACTCAGGTGTGCCCGA
CATGCGCGCCGTGGTGGTCGGCCACACGCCCCTGAAGCGCCCGGCGGCGTTGGGCAACGTCTATCACATCGACACGGGTG
GATGGCTGCGCGGCGGCAGCTTTACATTTCTGCGCCTTGATGACCTCCAGCCCTCGACGCCTGCGTCTCACGAACTGAAT
TGGGAGGGCGCTTGA

Upstream 100 bases:

>100_bases
GCGCGTCGCGCGAGGCGAAATTCGCGCGCTGGGATCTCGCATACGAGCGGTGGGCGCCCGTAAGGGCGAGAAAGATGGGA
ATTCAACTGCAGGAGGCTGC

Downstream 100 bases:

>100_bases
GGCTTGTCGTACGTATGCCGCTGCCGTCGTACCGAAAGGGTCGTCGATGGATGGAAAACTATCACTCGTTCGAATGCCTC
GTCGTTCGCGTGCGCGGGCC

Product: metallophosphoesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MITKYVRRFARNTVGIDYAVGDIHGCFAALEIELAKIRFDERVDRLFSVGDLVDRGPQSDTAVEWISKPWFHAVQGNHED
MAIRYVEPGNRDRDHYMMTGGAWLIEKPRYAQVMIADALAELPYAIEVETDGGLVGIVHADVHGKSWSDMVQAFACADSG
TKLRRITNSVLWNRDRIQDEDHSGVPDMRAVVVGHTPLKRPAALGNVYHIDTGGWLRGGSFTFLRLDDLQPSTPASHELN
WEGA

Sequences:

>Translated_244_residues
MITKYVRRFARNTVGIDYAVGDIHGCFAALEIELAKIRFDERVDRLFSVGDLVDRGPQSDTAVEWISKPWFHAVQGNHED
MAIRYVEPGNRDRDHYMMTGGAWLIEKPRYAQVMIADALAELPYAIEVETDGGLVGIVHADVHGKSWSDMVQAFACADSG
TKLRRITNSVLWNRDRIQDEDHSGVPDMRAVVVGHTPLKRPAALGNVYHIDTGGWLRGGSFTFLRLDDLQPSTPASHELN
WEGA
>Mature_244_residues
MITKYVRRFARNTVGIDYAVGDIHGCFAALEIELAKIRFDERVDRLFSVGDLVDRGPQSDTAVEWISKPWFHAVQGNHED
MAIRYVEPGNRDRDHYMMTGGAWLIEKPRYAQVMIADALAELPYAIEVETDGGLVGIVHADVHGKSWSDMVQAFACADSG
TKLRRITNSVLWNRDRIQDEDHSGVPDMRAVVVGHTPLKRPAALGNVYHIDTGGWLRGGSFTFLRLDDLQPSTPASHELN
WEGA

Specific function: Can hydrolyze phosphorylated Ser-, Thr- or Tyr- substrates in vitro. The natural substrate is unknown [H]

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPP phosphatase family. PP-1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081988, Length=213, Percent_Identity=33.8028169014084, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789090, Length=215, Percent_Identity=30.6976744186047, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 27301; Mature: 27301

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITKYVRRFARNTVGIDYAVGDIHGCFAALEIELAKIRFDERVDRLFSVGDLVDRGPQSD
CHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEHEEHHHHHHHHHHHHHHHHHHCCCCCC
TAVEWISKPWFHAVQGNHEDMAIRYVEPGNRDRDHYMMTGGAWLIEKPRYAQVMIADALA
HHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCEEEECCEEEEECCCHHHHHHHHHHH
ELPYAIEVETDGGLVGIVHADVHGKSWSDMVQAFACADSGTKLRRITNSVLWNRDRIQDE
HCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHCCCC
DHSGVPDMRAVVVGHTPLKRPAALGNVYHIDTGGWLRGGSFTFLRLDDLQPSTPASHELN
CCCCCCCCEEEEECCCCCCCCHHCCCEEEECCCCEEECCCEEEEEECCCCCCCCCCCCCC
WEGA
CCCC
>Mature Secondary Structure
MITKYVRRFARNTVGIDYAVGDIHGCFAALEIELAKIRFDERVDRLFSVGDLVDRGPQSD
CHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEHEEHHHHHHHHHHHHHHHHHHCCCCCC
TAVEWISKPWFHAVQGNHEDMAIRYVEPGNRDRDHYMMTGGAWLIEKPRYAQVMIADALA
HHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCEEEECCEEEEECCCHHHHHHHHHHH
ELPYAIEVETDGGLVGIVHADVHGKSWSDMVQAFACADSGTKLRRITNSVLWNRDRIQDE
HCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHCCCC
DHSGVPDMRAVVVGHTPLKRPAALGNVYHIDTGGWLRGGSFTFLRLDDLQPSTPASHELN
CCCCCCCCEEEEECCCCCCCCHHCCCEEEECCCCEEECCCEEEEEECCCCCCCCCCCCCC
WEGA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11717262; 11677609 [H]