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The map label for this gene is argT [H]

Identifier: 186476538

GI number: 186476538

Start: 2034780

End: 2035577

Strand: Reverse

Name: argT [H]

Synonym: Bphy_1780

Alternate gene names: 186476538

Gene position: 2035577-2034780 (Counterclockwise)

Preceding gene: 186476539

Following gene: 186476537

Centisome position: 58.51

GC content: 62.16

Gene sequence:

>798_bases
ATGAAATTGAGTTGGCAAAAGACGGCCGCGCTCGCCGCATTCGTAGGCGCTGCCGTGACAGCAACGGCACACGCAACCGC
AACCGAAATCAAGGAAATCCGCTTCGGCGTCGAAGCATCCTACGCGCCGTTCGAATCGAAGTCGCCCGCCGGCGACCTGC
AAGGCTTCGATATCGATATCGGCAACGCCGTCTGCGCCAAGCTCAAGACGAAGTGCGTATGGGTCGAGAATTCATTCGAC
GGCCTCATCCCCGCGCTCCAGGCCCGCAAGTTCAGCGCGATCAACTCCGATATGTCGATCACCGACCAGCGCCGCGCCGT
CATCGACTTCACCGATCCCATCTACGTGATCCCCAATCAGATGATCGCGAAGAAAGGCAGCGGCCTGCAGCCGACGCCCG
CTTCGCTCAAGGGCAAGCACGTCGGCGTGCTGCAAGGCACGATCCAGGAAACCTACGCGAAGGCGAAGTGGGCGCCCGCC
GGCGTCGACGTGGTTCCCTATCAGACGCAGGATCAGGTGTACGCAGACCTCGTCGCCGGCCGTCTCGATGCGTCGTTCCA
GGACGCGGAAGCGGCATCCAAGGGCTTCCTCAAGAAGCCGCAAGGCGCCGGCTTCGAATTCGCCGGGCCGGCCGTGTCCG
ATGAGAAGCTGCTCGGCTCGGGCGTCGGCTTCGGTCTGCGCAAGGGCGACGCCCAGCTAAAGGACGCCGTCAACCAGGCA
CTCAAGGAACTCAAAGCCGACGGCACCATCGACAAGCTCGCCGCGAAGTACTTCGACGTAAAAGTCGTGCTCAAGTAA

Upstream 100 bases:

>100_bases
CCGGCAATATCGTTTGAATCGCCGATTGGGCGCTGCAATGATCGTCCGCACGCCCAAGGCGGGGCATACCGCCGCCTGCA
CATCTTGAGGAAAGCACCCA

Downstream 100 bases:

>100_bases
GGCAAGCGTTATCCGTTGCGAAGCATCGCAGCGACAGCAGCACAACCGGCCGCCTCGAACGGCCGGTTGTGCTTTTTTGG
CCTTGTTTTATAGCCTTTTT

Product: cationic amino acid ABC transporter periplasmic binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKLSWQKTAALAAFVGAAVTATAHATATEIKEIRFGVEASYAPFESKSPAGDLQGFDIDIGNAVCAKLKTKCVWVENSFD
GLIPALQARKFSAINSDMSITDQRRAVIDFTDPIYVIPNQMIAKKGSGLQPTPASLKGKHVGVLQGTIQETYAKAKWAPA
GVDVVPYQTQDQVYADLVAGRLDASFQDAEAASKGFLKKPQGAGFEFAGPAVSDEKLLGSGVGFGLRKGDAQLKDAVNQA
LKELKADGTIDKLAAKYFDVKVVLK

Sequences:

>Translated_265_residues
MKLSWQKTAALAAFVGAAVTATAHATATEIKEIRFGVEASYAPFESKSPAGDLQGFDIDIGNAVCAKLKTKCVWVENSFD
GLIPALQARKFSAINSDMSITDQRRAVIDFTDPIYVIPNQMIAKKGSGLQPTPASLKGKHVGVLQGTIQETYAKAKWAPA
GVDVVPYQTQDQVYADLVAGRLDASFQDAEAASKGFLKKPQGAGFEFAGPAVSDEKLLGSGVGFGLRKGDAQLKDAVNQA
LKELKADGTIDKLAAKYFDVKVVLK
>Mature_265_residues
MKLSWQKTAALAAFVGAAVTATAHATATEIKEIRFGVEASYAPFESKSPAGDLQGFDIDIGNAVCAKLKTKCVWVENSFD
GLIPALQARKFSAINSDMSITDQRRAVIDFTDPIYVIPNQMIAKKGSGLQPTPASLKGKHVGVLQGTIQETYAKAKWAPA
GVDVVPYQTQDQVYADLVAGRLDASFQDAEAASKGFLKKPQGAGFEFAGPAVSDEKLLGSGVGFGLRKGDAQLKDAVNQA
LKELKADGTIDKLAAKYFDVKVVLK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=54.1501976284585, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=53.448275862069, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI1787085, Length=248, Percent_Identity=34.2741935483871, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1787088, Length=242, Percent_Identity=33.4710743801653, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1788228, Length=254, Percent_Identity=31.1023622047244, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787031, Length=251, Percent_Identity=29.0836653386454, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28156; Mature: 28156

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSWQKTAALAAFVGAAVTATAHATATEIKEIRFGVEASYAPFESKSPAGDLQGFDIDI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEC
GNAVCAKLKTKCVWVENSFDGLIPALQARKFSAINSDMSITDQRRAVIDFTDPIYVIPNQ
CHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEECCCH
MIAKKGSGLQPTPASLKGKHVGVLQGTIQETYAKAKWAPAGVDVVPYQTQDQVYADLVAG
HHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHH
RLDASFQDAEAASKGFLKKPQGAGFEFAGPAVSDEKLLGSGVGFGLRKGDAQLKDAVNQA
HCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHH
LKELKADGTIDKLAAKYFDVKVVLK
HHHHHCCCCHHHHHHHHHCEEEEEC
>Mature Secondary Structure
MKLSWQKTAALAAFVGAAVTATAHATATEIKEIRFGVEASYAPFESKSPAGDLQGFDIDI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEC
GNAVCAKLKTKCVWVENSFDGLIPALQARKFSAINSDMSITDQRRAVIDFTDPIYVIPNQ
CHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEECCCH
MIAKKGSGLQPTPASLKGKHVGVLQGTIQETYAKAKWAPAGVDVVPYQTQDQVYADLVAG
HHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHH
RLDASFQDAEAASKGFLKKPQGAGFEFAGPAVSDEKLLGSGVGFGLRKGDAQLKDAVNQA
HCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHH
LKELKADGTIDKLAAKYFDVKVVLK
HHHHHCCCCHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]