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The map label for this gene is amtB [C]

Identifier: 186476519

GI number: 186476519

Start: 2011206

End: 2012408

Strand: Direct

Name: amtB [C]

Synonym: Bphy_1761

Alternate gene names: 186476519

Gene position: 2011206-2012408 (Clockwise)

Preceding gene: 186476517

Following gene: 186476520

Centisome position: 57.81

GC content: 66.17

Gene sequence:

>1203_bases
ATGGACAATCTAAAAACGGGCACCGATACTCTCTTCCTTCTACTCGGCGCGGCGATGGTGCTAGCCATGCATGCGGGGTT
CGCGTTTCTCGAACTCGGCACGGTGCGCAAAAAGAATCAGGTCAACGCACTCGTGAAGATACTGGTCGACTTTTCGGTGT
CGACCCTCGCCTACTTCTTCATCGGCTACACCATAGCCTACGGCGTGCAGTTCTACGGCAGCGCGGAGACGCTCTCCCAG
CACAACGGCTATGCGCTCGTGCGCTTCTTCTTCCTGCTGACCTTCGCGGCGGCCATCCCCGCGATCGTCTCGGGCGGCAT
CGCGGAGCGCTCGAAGTTCAATCCGCAGCTCTTCGCGACCTTCGTGCTGGTCGGCTTCATCTATCCGTTCTTCGAAGGCA
TCGCGTGGAACGAGCGCTTCGGCATCCAGGCGTGGATCGCACAGACGTTCGGCGCGCCGTTTCATGACTTCGCCGGCTCC
GTCGTGGTGCACGCGTTCGGCGGCTGGGTTGCGCTGCCTGCCGTCATGCTGCTCGGCGCGCGCCACGGCCGCTATACGCG
CGACGGACGCATCGCCGCCCATCCGCCGTCGAACATTCCGTTCCTCGCGCTCGGCGCGTGGGTGCTGGCCGTCGGCTGGT
TCGGCTTCAACGTGATGAGCGCGCAGACGATCGACAAGATCAGCGGGCTCGTCGCCGTCAACTCGCTGATGGCAATGGTC
GGCGGCACGCTGACCGCGTGGCTCGCCGGCCGCAACGACCCGGGCTTCACCTACAACGGTCCGCTAGCCGGACTCGTGGC
CGTGTGCGCCGGCTCCGACCTGATGCATCCGCTCGGCGCGCTGGTGACGGGCGCCGTCGCGGGCGGGCTGTTCGTGTACA
TGTTCACGACCGTGCAGAACCGCTGGCGCATCGACGACGTGCTCGGCGTCTGGCCGCTGCATGGCCTGTGCGGCGCGTGG
GGCGGCATCGCGGCGGGTATCTTCGGCCAGCACGCGCTGGGCGGTCTCGGCGGCGTGTCGCTCACGGCGCAGCTGGTCGG
CACGCTCGGCGGCATCGTGTTCGCGACGCTCGGTGGCACGATCGTCTACGGCGCGATCCGGCTGACGGTCGGCCTGCGCA
TCGACCAGGAAAACGAATACAACGGCGCCGACCTGTCGATCCACAAGATCACGTCGACGCCGGAGCGCGAAACGGTCGGC
TGA

Upstream 100 bases:

>100_bases
TCCAGTGCGGACCCGCACCGACAGAACGCGACATTGCATCCATTTGGCCCATTTCTTGCGTTCCATGCCCGCTTTTCGCG
CATCGCAGGATCCTACACGG

Downstream 100 bases:

>100_bases
CGGGCTTTGCGCGGTGGGCCCACGCCGTGGCGGCCGCCCACCGCTGCGCGGCCGCGACGCCCCGTGTAAGCTCGCGTTCC
AGGGATCGGCCCCCGCCACC

Product: Rh family protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MDNLKTGTDTLFLLLGAAMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFSVSTLAYFFIGYTIAYGVQFYGSAETLSQ
HNGYALVRFFFLLTFAAAIPAIVSGGIAERSKFNPQLFATFVLVGFIYPFFEGIAWNERFGIQAWIAQTFGAPFHDFAGS
VVVHAFGGWVALPAVMLLGARHGRYTRDGRIAAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTIDKISGLVAVNSLMAMV
GGTLTAWLAGRNDPGFTYNGPLAGLVAVCAGSDLMHPLGALVTGAVAGGLFVYMFTTVQNRWRIDDVLGVWPLHGLCGAW
GGIAAGIFGQHALGGLGGVSLTAQLVGTLGGIVFATLGGTIVYGAIRLTVGLRIDQENEYNGADLSIHKITSTPERETVG

Sequences:

>Translated_400_residues
MDNLKTGTDTLFLLLGAAMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFSVSTLAYFFIGYTIAYGVQFYGSAETLSQ
HNGYALVRFFFLLTFAAAIPAIVSGGIAERSKFNPQLFATFVLVGFIYPFFEGIAWNERFGIQAWIAQTFGAPFHDFAGS
VVVHAFGGWVALPAVMLLGARHGRYTRDGRIAAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTIDKISGLVAVNSLMAMV
GGTLTAWLAGRNDPGFTYNGPLAGLVAVCAGSDLMHPLGALVTGAVAGGLFVYMFTTVQNRWRIDDVLGVWPLHGLCGAW
GGIAAGIFGQHALGGLGGVSLTAQLVGTLGGIVFATLGGTIVYGAIRLTVGLRIDQENEYNGADLSIHKITSTPERETVG
>Mature_400_residues
MDNLKTGTDTLFLLLGAAMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFSVSTLAYFFIGYTIAYGVQFYGSAETLSQ
HNGYALVRFFFLLTFAAAIPAIVSGGIAERSKFNPQLFATFVLVGFIYPFFEGIAWNERFGIQAWIAQTFGAPFHDFAGS
VVVHAFGGWVALPAVMLLGARHGRYTRDGRIAAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTIDKISGLVAVNSLMAMV
GGTLTAWLAGRNDPGFTYNGPLAGLVAVCAGSDLMHPLGALVTGAVAGGLFVYMFTTVQNRWRIDDVLGVWPLHGLCGAW
GGIAAGIFGQHALGGLGGVSLTAQLVGTLGGIVFATLGGTIVYGAIRLTVGLRIDQENEYNGADLSIHKITSTPERETVG

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=405, Percent_Identity=31.358024691358, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17550182, Length=437, Percent_Identity=29.7482837528604, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17550180, Length=444, Percent_Identity=29.2792792792793, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI32564042, Length=431, Percent_Identity=28.3062645011601, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI133931100, Length=437, Percent_Identity=25.858123569794, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6325396, Length=354, Percent_Identity=24.5762711864407, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321559, Length=419, Percent_Identity=25.2983293556086, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324187, Length=429, Percent_Identity=25.4079254079254, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI161078314, Length=418, Percent_Identity=28.7081339712919, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42379; Mature: 42379

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHEEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure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HHHHHEEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]