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The map label for this gene is rluB [H]

Identifier: 186476490

GI number: 186476490

Start: 1976972

End: 1978765

Strand: Reverse

Name: rluB [H]

Synonym: Bphy_1732

Alternate gene names: 186476490

Gene position: 1978765-1976972 (Counterclockwise)

Preceding gene: 186476491

Following gene: 186476489

Centisome position: 56.87

GC content: 70.18

Gene sequence:

>1794_bases
TTGACAGACATCCACGACACCGATTCGTCCGAATCCGAGCGCGCCGTCGCTGCTGCGCGCCCCGACGAAACGCGCAACGC
GAAAGCGTCAGCGGGCGATACCGAGCGGACCGGGCAAGCGCCGGACACCGAGGGCGACGAGCGCCCCCGCCGCGGCCTGC
GCCGTGGTCCGCGTAGCCTGATCGCGCGTCGCCGCGCGGGGGCAAAGGCAAAGACGGCCGATGCCGAGACTACGCAGCCG
GCGCCCGCCGTGACGGAAGCGCCGCCCGACGGCGAAGTCGTCGCGCCCATGCGCGCGCCGCGCAAGGACGCGGGCGCGAA
GGCGCAAGGCCAGGGCGGCCGTCGTGCCGGCGGCAAGCAGGGTGGCCAAAGCGGTCAACCGGGGGCTCGCCAGGGCGGCC
AGCGCAAGGGCCGCGGCAATGCGGCCGGCGATGCCGCGCCTGCAGCCACCGAAGGCGACAAGCAGGACGACCTGTTCGCC
TATGTGACGTCGCCCGCCTTCGACGCCGACAACTCGGCAACGGGCGGTGTGCGCGCGCCGATGCTGCGCCGCGGCCGTCC
CGCCGCGCCTAAGCGCGTTCTGTCGCCGGACGACGACGCGCCCAAGCTGCACAAGGTGCTCGCCGACGCCGGCATGGGCT
CGCGTCGCGACATGGAAGAGCTGATCGTCGCGGGTCGCGTGTCGGTGAACGGCGAGCCGGCGCACATCGGTCAGCGCATC
CTGCCTACCGACCTCGTACGCATCAATGGCAAGCCGATCAAGCGCAAGCTGCAGAGCAAGCCGCCGCGCGTGCTGCTGTA
TCACAAGCCGACGGGCGAAATCGTCAGCCATGCCGATCCGGAAGGCCGTCCGTCCGTGTTCGACAAGCTGCCGCCGATGA
AGACGGCGAAATGGCTTGCCGTCGGTCGTCTCGACTTCAATACGGAAGGTCTGCTGATGCTGACGACGTCGGGCGATCTG
GCGAACCGGTTCATGCATCCGCGTTACAGCGTCGAACGCGAATATGCGGTGCGCGTGGTCGGCGAGCTGACGGAAGGCAA
CCGGCAGAAACTGTTGCACGGCGTCGAACTCGACGACGGTCCGGCGAACTTCCTGCGCATCCGCGACGGCGGCGGCGAAG
GGACGAACCACTGGTATCACGTCGCGCTCGCCGAAGGTCGTAACCGCGAAGTGCGCCGCATGTTCGAAGCGGTCGGCCTG
ATGGTGAGCCGGCTGATCCGTACGCGTCACGGCCCGATCGCGCTGCCGCGCGGTCTGAAGCGTGGGCGCTGGGAAGAGCT
CGAGGACAACCAGGTGCGCAGCCTGATGGCGTCGGTGGGTTTGAAGGCGCCGGCAGAAGAGAAGGGCGCCGGTCGCCGTG
GCGAGCCCGAGCGCCGTCAACCGGATCCGATGCAGACCTCGATGGGCTTCATCAATCGCGAACCCGTGCTGATGTCGCAT
GGCCGTTTCGACCAGCAGCAGCCACGCGGCGGCGGTCCTGGTCGCCGCGGCGGCAAGGGCGCAGGCGCATTCGGCGCGCT
GAACACCGGCTTGGGCGGCGGCTTCGGCAATACGAACGGCAACCGCGGCGAAGGCCGTGGCGGCCGGGGTGGCCGCGATG
TCGACGGCAATCGGGCGCCGTCGGGCAACCGTGCGGCCGCAGGCGGCGGTAATGCGAAGCGTGGCGGTGGCGGCGCTGGT
AATCGCGCGCAAAACGGCAACCGCGCGGGCAACGGCAATCGCGCGGGCGGCAATCCGAACGCTGGCGGCAATCGCAGCGG
CGGCGCGCAGCGCAACCGTTCCCGCGGACGCTGA

Upstream 100 bases:

>100_bases
CGAATTCTCCGACGACGAAGCCGAGTCGCGCAGCGCCTGACGTAACCGTACTTTTTGACGCGCCCGCAATGGGCGCGCGA
CCTGACATTTTGAGGTTGTT

Downstream 100 bases:

>100_bases
TCGTCACGAAGCGGCGGCGGCGCCCGTAGCCGCCGCCGAGTTCGACTGCATCGCGCGCATCGGAGATTGTCTTGCGCATC
CGCCAGCGAACCATCTCTCG

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MTDIHDTDSSESERAVAAARPDETRNAKASAGDTERTGQAPDTEGDERPRRGLRRGPRSLIARRRAGAKAKTADAETTQP
APAVTEAPPDGEVVAPMRAPRKDAGAKAQGQGGRRAGGKQGGQSGQPGARQGGQRKGRGNAAGDAAPAATEGDKQDDLFA
YVTSPAFDADNSATGGVRAPMLRRGRPAAPKRVLSPDDDAPKLHKVLADAGMGSRRDMEELIVAGRVSVNGEPAHIGQRI
LPTDLVRINGKPIKRKLQSKPPRVLLYHKPTGEIVSHADPEGRPSVFDKLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDL
ANRFMHPRYSVEREYAVRVVGELTEGNRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGL
MVSRLIRTRHGPIALPRGLKRGRWEELEDNQVRSLMASVGLKAPAEEKGAGRRGEPERRQPDPMQTSMGFINREPVLMSH
GRFDQQQPRGGGPGRRGGKGAGAFGALNTGLGGGFGNTNGNRGEGRGGRGGRDVDGNRAPSGNRAAAGGGNAKRGGGGAG
NRAQNGNRAGNGNRAGGNPNAGGNRSGGAQRNRSRGR

Sequences:

>Translated_597_residues
MTDIHDTDSSESERAVAAARPDETRNAKASAGDTERTGQAPDTEGDERPRRGLRRGPRSLIARRRAGAKAKTADAETTQP
APAVTEAPPDGEVVAPMRAPRKDAGAKAQGQGGRRAGGKQGGQSGQPGARQGGQRKGRGNAAGDAAPAATEGDKQDDLFA
YVTSPAFDADNSATGGVRAPMLRRGRPAAPKRVLSPDDDAPKLHKVLADAGMGSRRDMEELIVAGRVSVNGEPAHIGQRI
LPTDLVRINGKPIKRKLQSKPPRVLLYHKPTGEIVSHADPEGRPSVFDKLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDL
ANRFMHPRYSVEREYAVRVVGELTEGNRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGL
MVSRLIRTRHGPIALPRGLKRGRWEELEDNQVRSLMASVGLKAPAEEKGAGRRGEPERRQPDPMQTSMGFINREPVLMSH
GRFDQQQPRGGGPGRRGGKGAGAFGALNTGLGGGFGNTNGNRGEGRGGRGGRDVDGNRAPSGNRAAAGGGNAKRGGGGAG
NRAQNGNRAGNGNRAGGNPNAGGNRSGGAQRNRSRGR
>Mature_596_residues
TDIHDTDSSESERAVAAARPDETRNAKASAGDTERTGQAPDTEGDERPRRGLRRGPRSLIARRRAGAKAKTADAETTQPA
PAVTEAPPDGEVVAPMRAPRKDAGAKAQGQGGRRAGGKQGGQSGQPGARQGGQRKGRGNAAGDAAPAATEGDKQDDLFAY
VTSPAFDADNSATGGVRAPMLRRGRPAAPKRVLSPDDDAPKLHKVLADAGMGSRRDMEELIVAGRVSVNGEPAHIGQRIL
PTDLVRINGKPIKRKLQSKPPRVLLYHKPTGEIVSHADPEGRPSVFDKLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLA
NRFMHPRYSVEREYAVRVVGELTEGNRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLM
VSRLIRTRHGPIALPRGLKRGRWEELEDNQVRSLMASVGLKAPAEEKGAGRRGEPERRQPDPMQTSMGFINREPVLMSHG
RFDQQQPRGGGPGRRGGKGAGAFGALNTGLGGGFGNTNGNRGEGRGGRGGRDVDGNRAPSGNRAAAGGGNAKRGGGGAGN
RAQNGNRAGNGNRAGGNPNAGGNRSGGAQRNRSRGR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=254, Percent_Identity=54.3307086614173, Blast_Score=262, Evalue=6e-71,
Organism=Escherichia coli, GI1790453, Length=242, Percent_Identity=29.7520661157025, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87081838, Length=192, Percent_Identity=34.8958333333333, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1788510, Length=217, Percent_Identity=30.8755760368664, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 63150; Mature: 63019

Theoretical pI: Translated: 11.48; Mature: 11.48

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIHDTDSSESERAVAAARPDETRNAKASAGDTERTGQAPDTEGDERPRRGLRRGPRSL
CCCCCCCCCCCCCCEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHH
IARRRAGAKAKTADAETTQPAPAVTEAPPDGEVVAPMRAPRKDAGAKAQGQGGRRAGGKQ
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHCCCCCCCCCCCCCCCCC
GGQSGQPGARQGGQRKGRGNAAGDAAPAATEGDKQDDLFAYVTSPAFDADNSATGGVRAP
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCH
MLRRGRPAAPKRVLSPDDDAPKLHKVLADAGMGSRRDMEELIVAGRVSVNGEPAHIGQRI
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCEEECCCCHHHHCCCC
LPTDLVRINGKPIKRKLQSKPPRVLLYHKPTGEIVSHADPEGRPSVFDKLPPMKTAKWLA
CCCCEEEECCCHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCHHHHCCCCCCCCCEEE
VGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYAVRVVGELTEGNRQKLLHGVELDDG
EEEEEECCCCEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCC
PANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIALPRGLK
CCEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RGRWEELEDNQVRSLMASVGLKAPAEEKGAGRRGEPERRQPDPMQTSMGFINREPVLMSH
CCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEC
GRFDQQQPRGGGPGRRGGKGAGAFGALNTGLGGGFGNTNGNRGEGRGGRGGRDVDGNRAP
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SGNRAAAGGGNAKRGGGGAGNRAQNGNRAGNGNRAGGNPNAGGNRSGGAQRNRSRGR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDIHDTDSSESERAVAAARPDETRNAKASAGDTERTGQAPDTEGDERPRRGLRRGPRSL
CCCCCCCCCCCCCEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHH
IARRRAGAKAKTADAETTQPAPAVTEAPPDGEVVAPMRAPRKDAGAKAQGQGGRRAGGKQ
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHCCCCCCCCCCCCCCCCC
GGQSGQPGARQGGQRKGRGNAAGDAAPAATEGDKQDDLFAYVTSPAFDADNSATGGVRAP
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCH
MLRRGRPAAPKRVLSPDDDAPKLHKVLADAGMGSRRDMEELIVAGRVSVNGEPAHIGQRI
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCEEECCCCHHHHCCCC
LPTDLVRINGKPIKRKLQSKPPRVLLYHKPTGEIVSHADPEGRPSVFDKLPPMKTAKWLA
CCCCEEEECCCHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCHHHHCCCCCCCCCEEE
VGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYAVRVVGELTEGNRQKLLHGVELDDG
EEEEEECCCCEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCC
PANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIALPRGLK
CCEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RGRWEELEDNQVRSLMASVGLKAPAEEKGAGRRGEPERRQPDPMQTSMGFINREPVLMSH
CCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEC
GRFDQQQPRGGGPGRRGGKGAGAFGALNTGLGGGFGNTNGNRGEGRGGRGGRDVDGNRAP
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SGNRAAAGGGNAKRGGGGAGNRAQNGNRAGNGNRAGGNPNAGGNRSGGAQRNRSRGR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]