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The map label for this gene is sucB [H]

Identifier: 186476478

GI number: 186476478

Start: 1957887

End: 1959158

Strand: Reverse

Name: sucB [H]

Synonym: Bphy_1720

Alternate gene names: 186476478

Gene position: 1959158-1957887 (Counterclockwise)

Preceding gene: 186476479

Following gene: 186476477

Centisome position: 56.31

GC content: 64.23

Gene sequence:

>1272_bases
ATGGCTATTGTTGAAGTCAAGGTCCCCCAGCTGTCCGAGTCGGTCTCGGAAGCCACGATGCTGCAGTGGAAGAAGAAGCC
GGGTGAGGCTGTCGCCCAGGACGAAATCCTGATCGAAATCGAAACCGACAAGGTCGTGCTCGAAGTGCCGGCTCCGTCGG
CAGGCGTGCTCGCGCAAGTCATCAAGAACGACGGCGACATTGTCACGGCCGACGAAGTGATCGCGAAGATCGACACGGAA
GGCAAGCCGGGCGCCGCGGCGGTCGAAGCCGAAGTGAAGCCGGCTCCGCAAGCTGAACCGGTTGCTGCCGCCGCGGCGCA
AGCGGCAGCCGTGGCCGGCGCAAGCACGGCGGCATCGCCGGCTGCGACGAAGATCCTGGCCGAGAAGGGCGTGGCTGCGA
GCGACGTCGCTGGCACGGGCCGCGATGGCCGCATCACCAAGCAGGACGCCGTCGCCGCCGGCGCACCGGCAGCCAAGGCT
GCACCGGCACCGGCAGCCGCGCCCGCACGGGCTGCGAAACCGTCGCTGCCGCAAGTGGGCGCACCGGCATCGGCCGAGCA
ATGGCTGAAGGACCGCCCGGAACAGCGCGTCCCGATGTCGCGTCTGCGTGCGCGTATCGCTGAGCGCCTGCTCGAGTCGC
AGCAAACCAACGCCATCCTCACGACGTTCAACGAAGTGAACATGGCGCCCGTCATGGATCTGCGCAACAAGTACAAGGAC
AAGTTCGAGAAGGAACATGGCGTGAAGCTTGGCTTCATGTCGTTCTTCGTGAAGGCGGCTGTCCACGCGCTGAAGAAGTT
CCCGCTAGTGAACGCGTCGATCGACGGGAACGACATCGTCTACCACGGCTACTTCGACATCGGTATTGCCGTCGGTTCGC
CGCGCGGTCTGGTGGTGCCGATCCTGCGCAACGCCGACCAGATGAGCCTCGCCGATATCGAGAAGAAGATCGCCGAATTC
GGCCAGAAGGCGAAGGACGGCAAGCTGTCGATCGAAGAAATGACGGGCGGCACGTTCTCGATTTCGAACGGCGGCGTGTT
CGGCTCGATGCTGTCGACGCCGATCATCAACCCGCCGCAGTCGGCCATTCTCGGCGTGCACGCAACGAAGGAGCGCGCGG
TCGTCGAAAACGGTCAGATCGTGATCCGTCCGATGAACTATCTGGCGCTGTCGTACGACCACCGCATCATCGACGGCCGC
GAAGCGGTGCTGTCGCTGGTCGCGATGAAGGACGCGCTGGAAGATCCCGCACGCCTGCTGCTCGACCTGTAA

Upstream 100 bases:

>100_bases
CCGCGTCTGAATCTCCCATGTAAGCGCGACACGGCACGCGCACATCGGACGCGGCTCGCACAAGGTTCGTTGTCATCCGA
TACGTATTCAGAGAAATCAA

Downstream 100 bases:

>100_bases
TGCGCGATACGCGACGGCGGCTTGAAGTCGGAGCCCGCCGTCGCGATATACGCATCAGGTGTCTTTCGCATTTCCGCAGT
ATCTGAAGCCACGCGCGCCG

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLAQVIKNDGDIVTADEVIAKIDTE
GKPGAAAVEAEVKPAPQAEPVAAAAAQAAAVAGASTAASPAATKILAEKGVAASDVAGTGRDGRITKQDAVAAGAPAAKA
APAPAAAPARAAKPSLPQVGAPASAEQWLKDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMDLRNKYKD
KFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSLADIEKKIAEF
GQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERAVVENGQIVIRPMNYLALSYDHRIIDGR
EAVLSLVAMKDALEDPARLLLDL

Sequences:

>Translated_423_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLAQVIKNDGDIVTADEVIAKIDTE
GKPGAAAVEAEVKPAPQAEPVAAAAAQAAAVAGASTAASPAATKILAEKGVAASDVAGTGRDGRITKQDAVAAGAPAAKA
APAPAAAPARAAKPSLPQVGAPASAEQWLKDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMDLRNKYKD
KFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSLADIEKKIAEF
GQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERAVVENGQIVIRPMNYLALSYDHRIIDGR
EAVLSLVAMKDALEDPARLLLDL
>Mature_422_residues
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLAQVIKNDGDIVTADEVIAKIDTEG
KPGAAAVEAEVKPAPQAEPVAAAAAQAAAVAGASTAASPAATKILAEKGVAASDVAGTGRDGRITKQDAVAAGAPAAKAA
PAPAAAPARAAKPSLPQVGAPASAEQWLKDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMDLRNKYKDK
FEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSLADIEKKIAEFG
QKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERAVVENGQIVIRPMNYLALSYDHRIIDGRE
AVLSLVAMKDALEDPARLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=256, Percent_Identity=53.90625, Blast_Score=285, Evalue=5e-77,
Organism=Homo sapiens, GI31711992, Length=447, Percent_Identity=30.4250559284116, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI110671329, Length=446, Percent_Identity=28.9237668161435, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI203098753, Length=463, Percent_Identity=29.8056155507559, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI203098816, Length=463, Percent_Identity=29.8056155507559, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=36.6459627329193, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1786946, Length=423, Percent_Identity=52.4822695035461, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1786305, Length=430, Percent_Identity=31.6279069767442, Blast_Score=164, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=421, Percent_Identity=43.4679334916865, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI17560088, Length=453, Percent_Identity=30.9050772626932, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=436, Percent_Identity=25.9174311926606, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=31.5112540192926, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=421, Percent_Identity=42.9928741092637, Blast_Score=336, Evalue=5e-93,
Organism=Saccharomyces cerevisiae, GI6324258, Length=460, Percent_Identity=29.7826086956522, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24645909, Length=242, Percent_Identity=56.198347107438, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI18859875, Length=450, Percent_Identity=28, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=33.7719298245614, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=33.7719298245614, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44476; Mature: 44344

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLAQV
CEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHH
IKNDGDIVTADEVIAKIDTEGKPGAAAVEAEVKPAPQAEPVAAAAAQAAAVAGASTAASP
HHCCCCEEEHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCH
AATKILAEKGVAASDVAGTGRDGRITKQDAVAAGAPAAKAAPAPAAAPARAAKPSLPQVG
HHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APASAEQWLKDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMDLRNKYKD
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHH
KFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVP
HHHHHHCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEEE
ILRNADQMSLADIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQ
EECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCCCCCCCCH
SAILGVHATKERAVVENGQIVIRPMNYLALSYDHRIIDGREAVLSLVAMKDALEDPARLL
HHEEEEECCCCCCEEECCCEEEEEHHHEEEECCCEEECCHHHHHHHHHHHHHHCCHHHHH
LDL
HCC
>Mature Secondary Structure 
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLAQV
EEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHH
IKNDGDIVTADEVIAKIDTEGKPGAAAVEAEVKPAPQAEPVAAAAAQAAAVAGASTAASP
HHCCCCEEEHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCH
AATKILAEKGVAASDVAGTGRDGRITKQDAVAAGAPAAKAAPAPAAAPARAAKPSLPQVG
HHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APASAEQWLKDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMDLRNKYKD
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHH
KFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVP
HHHHHHCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEEE
ILRNADQMSLADIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQ
EECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCCCCCCCCH
SAILGVHATKERAVVENGQIVIRPMNYLALSYDHRIIDGREAVLSLVAMKDALEDPARLL
HHEEEEECCCCCCEEECCCEEEEEHHHEEEECCCEEECCHHHHHHHHHHHHHHCCHHHHH
LDL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]