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The map label for this gene is odhL [H]

Identifier: 186476477

GI number: 186476477

Start: 1956318

End: 1957748

Strand: Reverse

Name: odhL [H]

Synonym: Bphy_1719

Alternate gene names: 186476477

Gene position: 1957748-1956318 (Counterclockwise)

Preceding gene: 186476478

Following gene: 186476476

Centisome position: 56.27

GC content: 62.96

Gene sequence:

>1431_bases
ATGTCCAAAGAATTTGACGTCGTCGTGATCGGTGCCGGTCCCGGCGGCTACATCGCGGCCATTCGCGCTGCGCAGCTCGG
CAAGACGGTTGCCTGTATCGAAAAGTGGAAGAACCCGGCCGGCGCGCTGAAGCTGGGCGGCACGTGCCTGAACGTCGGCT
GCATTCCGTCGAAGGCGCTGCTCGCGTCGTCGGAAGAATTCGAGAACGCATCGCATCACCTCGCGGACCACGGCATCAGC
GTGGAGAACGTGAAGGTCGACATCGCGAAGATGCTGGCCCGCAAGGAAGGCATCGTCGAGAAGATGACGAAGGGCATCGA
GTTCCTGTTCCGCAAGAACAAGATCACGTGGCTCAAGGGTCATGGCAAGTTCACGGGCAAGACGGACGCCGGCGTGCAGA
TCGAAGTGTCGGGCGAAGGCGAGACGGAAGTGGTCACGGCGAAGAACGTGATCATTGCGACGGGCTCGAAGGCGCGTCAC
CTGCCGAACGTGCCCGTCGACAACAAGATCGTCGCGGACAACGAAGGCGCACTGTCGTTCACCGAAGTGCCGAAGAAGCT
CGCCGTGATCGGCGCGGGCGTGATCGGCCTGGAGTTGGGCTCGGTGTGGCGCCGCCTGGGTGCGGAAGTGACCGTGCTGG
AAGCGCTGCCCGAGTTCCTCGGCGCCGCCGACCAGGCGCTCGCGAAGGAAGCCGCGAAGCAGTTCAAGAAGCAGGGTCTC
GACATCCACGTCGGCGTGAAGGTCGGCGAAGTCAAGACCACGGCCAACAGCGTGTCGATCTCGTACACGGACAAGGACGG
TAACGCGAAGACGCTCGACGCGGACCGCCTGATCGTGTCGATCGGCCGCGTGCCGAACACCGATAACCTGGGCCTCGAAG
CGATTGGCCTGAAGGCGAACGAGCGCGGCTTCATCGACGTGGATGACCACTGTGCAACGTCGGTGCCGAACGTGTACGCG
ATCGGCGACGTGGTGCGTGGCCCGATGCTCGCCCACAAGGCCGAAGACGAAGGCGTGCTGGTCGCGGAAGTGATCGACGG
TCAGAAGCCGCACATCGATTACAACTGCGTGCCGTGGGTCATCTACACGGAACCGGAAATCGCGTGGGTCGGCAAGACGG
AACAGCAGCTCAAGGCGGAAGGCCGCGAGATCAAGTCCGGCCAGTTCCCGTTCATGGCGAACGGCCGCGCACTCGGCATC
AACAAGGCGGATGGCTTCGTCAAGATGATCGCCGACGCGAAGACGGACGAACTGCTCGGCGTGCACATCATCGCTGCCAA
CGCGTCGGATCTGATCGCCGAAGCCGTGGTCGCGATGGAGTTCAAGGCTGCGTCGGAAGACATCGGCCGTATCTGCCATC
CGCACCCGTCGTTGTCCGAAGTGATGCGTGAAGCGGCGCTCGCCGTCGATAAGCGCGCGCTGAACATGTAA

Upstream 100 bases:

>100_bases
CGTCGCGATATACGCATCAGGTGTCTTTCGCATTTCCGCAGTATCTGAAGCCACGCGCGCCGCGAAGCGCGGCCGCGTCG
GTTAGCCAAAAAGGATAGTC

Downstream 100 bases:

>100_bases
GACGCATCTGCCCGAATCTGATTCGGGGCACACGCGAATCACGACGAAGGCGGGTGGGTTGAATCCCTCCCGCCTTCTTC
ATTGCTGCCTCACGAAATGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKALLASSEEFENASHHLADHGIS
VENVKVDIAKMLARKEGIVEKMTKGIEFLFRKNKITWLKGHGKFTGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARH
LPNVPVDNKIVADNEGALSFTEVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPEFLGAADQALAKEAAKQFKKQGL
DIHVGVKVGEVKTTANSVSISYTDKDGNAKTLDADRLIVSIGRVPNTDNLGLEAIGLKANERGFIDVDDHCATSVPNVYA
IGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCVPWVIYTEPEIAWVGKTEQQLKAEGREIKSGQFPFMANGRALGI
NKADGFVKMIADAKTDELLGVHIIAANASDLIAEAVVAMEFKAASEDIGRICHPHPSLSEVMREAALAVDKRALNM

Sequences:

>Translated_476_residues
MSKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKALLASSEEFENASHHLADHGIS
VENVKVDIAKMLARKEGIVEKMTKGIEFLFRKNKITWLKGHGKFTGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARH
LPNVPVDNKIVADNEGALSFTEVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPEFLGAADQALAKEAAKQFKKQGL
DIHVGVKVGEVKTTANSVSISYTDKDGNAKTLDADRLIVSIGRVPNTDNLGLEAIGLKANERGFIDVDDHCATSVPNVYA
IGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCVPWVIYTEPEIAWVGKTEQQLKAEGREIKSGQFPFMANGRALGI
NKADGFVKMIADAKTDELLGVHIIAANASDLIAEAVVAMEFKAASEDIGRICHPHPSLSEVMREAALAVDKRALNM
>Mature_475_residues
SKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKALLASSEEFENASHHLADHGISV
ENVKVDIAKMLARKEGIVEKMTKGIEFLFRKNKITWLKGHGKFTGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARHL
PNVPVDNKIVADNEGALSFTEVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPEFLGAADQALAKEAAKQFKKQGLD
IHVGVKVGEVKTTANSVSISYTDKDGNAKTLDADRLIVSIGRVPNTDNLGLEAIGLKANERGFIDVDDHCATSVPNVYAI
GDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCVPWVIYTEPEIAWVGKTEQQLKAEGREIKSGQFPFMANGRALGIN
KADGFVKMIADAKTDELLGVHIIAANASDLIAEAVVAMEFKAASEDIGRICHPHPSLSEVMREAALAVDKRALNM

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=48.1798715203426, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI50301238, Length=470, Percent_Identity=28.7234042553192, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI33519430, Length=469, Percent_Identity=26.865671641791, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI33519428, Length=469, Percent_Identity=26.865671641791, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI33519426, Length=469, Percent_Identity=26.865671641791, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI148277065, Length=469, Percent_Identity=26.865671641791, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI148277071, Length=469, Percent_Identity=26.865671641791, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI22035672, Length=474, Percent_Identity=27.8481012658228, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI291045266, Length=461, Percent_Identity=26.2472885032538, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI291045268, Length=456, Percent_Identity=24.780701754386, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1786307, Length=468, Percent_Identity=39.5299145299145, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI87082354, Length=475, Percent_Identity=30.5263157894737, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1789915, Length=466, Percent_Identity=29.8283261802575, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87081717, Length=467, Percent_Identity=28.2655246252677, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI87081763, Length=191, Percent_Identity=31.413612565445, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=469, Percent_Identity=48.4008528784648, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17557007, Length=494, Percent_Identity=28.3400809716599, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71982272, Length=480, Percent_Identity=26.6666666666667, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI71983419, Length=467, Percent_Identity=27.4089935760171, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71983429, Length=467, Percent_Identity=27.4089935760171, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17559934, Length=227, Percent_Identity=27.3127753303965, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=45.0207468879668, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=30.188679245283, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6325166, Length=472, Percent_Identity=27.9661016949153, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21358499, Length=470, Percent_Identity=49.7872340425532, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24640553, Length=490, Percent_Identity=28.3673469387755, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24640549, Length=490, Percent_Identity=28.3673469387755, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24640551, Length=490, Percent_Identity=28.3673469387755, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17737741, Length=487, Percent_Identity=25.2566735112936, Blast_Score=126, Evalue=4e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50628; Mature: 50497

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKAL
CCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEECCEEEEECCCCCHHH
LASSEEFENASHHLADHGISVENVKVDIAKMLARKEGIVEKMTKGIEFLFRKNKITWLKG
HCCCHHHCCHHHHHHHCCCCEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
HGKFTGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARHLPNVPVDNKIVADNEGALSF
CCEECCCCCCCEEEEECCCCCEEEEEECEEEEECCCCCCCCCCCCCCCEEEECCCCCEEH
TEVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPEFLGAADQALAKEAAKQFKKQGL
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHCHHHHHHHHHHHHHHHHCCC
DIHVGVKVGEVKTTANSVSISYTDKDGNAKTLDADRLIVSIGRVPNTDNLGLEAIGLKAN
EEEEEEEECCEEECCCEEEEEEECCCCCCEEECCCEEEEEECCCCCCCCCCEEEEEEECC
ERGFIDVDDHCATSVPNVYAIGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCVPWV
CCCCEEECCCHHCCCCCEEEEHHHHCCCHHEECCCCCCEEEEEECCCCCCCCCCCCEEEE
IYTEPEIAWVGKTEQQLKAEGREIKSGQFPFMANGRALGINKADGFVKMIADAKTDELLG
EEECCCEEEECCCHHHHHHCCCCCCCCCCCEEECCEEEECCCCCCHHEEECCCCCCCEEE
VHIIAANASDLIAEAVVAMEFKAASEDIGRICHPHPSLSEVMREAALAVDKRALNM
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKAL
CCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEECCEEEEECCCCCHHH
LASSEEFENASHHLADHGISVENVKVDIAKMLARKEGIVEKMTKGIEFLFRKNKITWLKG
HCCCHHHCCHHHHHHHCCCCEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
HGKFTGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARHLPNVPVDNKIVADNEGALSF
CCEECCCCCCCEEEEECCCCCEEEEEECEEEEECCCCCCCCCCCCCCCEEEECCCCCEEH
TEVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPEFLGAADQALAKEAAKQFKKQGL
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHCHHHHHHHHHHHHHHHHCCC
DIHVGVKVGEVKTTANSVSISYTDKDGNAKTLDADRLIVSIGRVPNTDNLGLEAIGLKAN
EEEEEEEECCEEECCCEEEEEEECCCCCCEEECCCEEEEEECCCCCCCCCCEEEEEEECC
ERGFIDVDDHCATSVPNVYAIGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCVPWV
CCCCEEECCCHHCCCCCEEEEHHHHCCCHHEECCCCCCEEEEEECCCCCCCCCCCCEEEE
IYTEPEIAWVGKTEQQLKAEGREIKSGQFPFMANGRALGINKADGFVKMIADAKTDELLG
EEECCCEEEECCCHHHHHHCCCCCCCCCCCEEECCEEEECCCCCCHHEEECCCCCCCEEE
VHIIAANASDLIAEAVVAMEFKAASEDIGRICHPHPSLSEVMREAALAVDKRALNM
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]