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The map label for this gene is dusA [H]

Identifier: 186476399

GI number: 186476399

Start: 1862685

End: 1863686

Strand: Reverse

Name: dusA [H]

Synonym: Bphy_1641

Alternate gene names: 186476399

Gene position: 1863686-1862685 (Counterclockwise)

Preceding gene: 186476401

Following gene: 186476396

Centisome position: 53.57

GC content: 63.27

Gene sequence:

>1002_bases
ATGTCTTCAAAGCCCAATGTCAGTGCCCGCCGCGTGTCCGTCGCGCCCATGATGGACTGGACCGACCGCCATTGCCGCTC
GTTTCACCGCACGCTGTCCCGGCACGCCTGGCTGTACACAGAGATGGTGACCACAGGGGCGCTGATTCACGGGGACGTCG
CGCGTCATCTTGCGTTTACGGCTGATGAAGCGCCCGTCGCGTTGCAGCTCGGCGGCAGCGAGCGGGAAGATCTGGCGAGG
TCGGCCAAGCTCGGCGAACAATGGGGCTACGACGAAATCAATCTGAATTGCGGCTGCCCGTCGGAGCGCGTGCAGCGCGG
CGCATTCGGCGCATGCCTGATGAAGGAGCCGGAACTCGTCGCCGATTGCGTGAAGGCAATGCGCGATGCGGTGTCGGTGC
CCGTGACCGTCAAGCACCGGATCGGCGTCGACGATGTCGAAGACTACGCGTTCGTGCGCGACTTCGTCGGCACGATCGCC
GACGCAGGTTGTGACGTGTTCATCGTGCACGCGCGCAACGCGATCCTCAAAGGCCTGAGCCCGAAAGAGAATCGCGAGAT
CCCGCCGCTCAAGTACGACTACGCGTATCGCCTGAAGCGCGACTTTCCGCAGCTCGAAATCATCATCAATGGTGGCATCA
AGACGCTGGATGAAGTCGAAACGCACCTGCAGCACGTCGACGGCGTGATGCTGGGCCGCGAGGCGTATCACAACCCATTC
GTGCTCGCGGATGTCGATGCGCGCTTCTACGGTTCGACGGATGCCGCGCCGACGCGCGAAGAAGCCGAGGCGCAATTGAT
CGAATATTGCCGCGCCGAGCTCGCGCGCGGTACCTACCTTGGCGCGATCGTGCGCCATGCGCTGGGCCTGTATCGCGGCG
TTGCGGGCGCGCGCGGATGGCGGCGGGTGCTGTCCGATAACAAGAAGCTGGCGGCACGCGACCTCGCGATTTTCGACGAA
GCGCGACAGCATTTGCGCGAGCCCAGCGAAATGTTTGAATAA

Upstream 100 bases:

>100_bases
GGTGAAGTGCAGGGAAGGGGGCACCGGAGGCGGCTTGCGGTAAAATTGCGCGCTATCCGAGTGGTTCGTTTGTCGTTTCT
TATTTCTGGTTTGCCGCCTC

Downstream 100 bases:

>100_bases
AGGGCTTTGCAAATACGATTCGTGTTTGTATAATCTCGCTTCTTCGTTGCTGACCGCCGCTCGGTAGCAGGCAGCGGAGG
TGTAGTTCAGTGGTGGCTGT

Product: YjbN family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSSKPNVSARRVSVAPMMDWTDRHCRSFHRTLSRHAWLYTEMVTTGALIHGDVARHLAFTADEAPVALQLGGSEREDLAR
SAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMKEPELVADCVKAMRDAVSVPVTVKHRIGVDDVEDYAFVRDFVGTIA
DAGCDVFIVHARNAILKGLSPKENREIPPLKYDYAYRLKRDFPQLEIIINGGIKTLDEVETHLQHVDGVMLGREAYHNPF
VLADVDARFYGSTDAAPTREEAEAQLIEYCRAELARGTYLGAIVRHALGLYRGVAGARGWRRVLSDNKKLAARDLAIFDE
ARQHLREPSEMFE

Sequences:

>Translated_333_residues
MSSKPNVSARRVSVAPMMDWTDRHCRSFHRTLSRHAWLYTEMVTTGALIHGDVARHLAFTADEAPVALQLGGSEREDLAR
SAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMKEPELVADCVKAMRDAVSVPVTVKHRIGVDDVEDYAFVRDFVGTIA
DAGCDVFIVHARNAILKGLSPKENREIPPLKYDYAYRLKRDFPQLEIIINGGIKTLDEVETHLQHVDGVMLGREAYHNPF
VLADVDARFYGSTDAAPTREEAEAQLIEYCRAELARGTYLGAIVRHALGLYRGVAGARGWRRVLSDNKKLAARDLAIFDE
ARQHLREPSEMFE
>Mature_332_residues
SSKPNVSARRVSVAPMMDWTDRHCRSFHRTLSRHAWLYTEMVTTGALIHGDVARHLAFTADEAPVALQLGGSEREDLARS
AKLGEQWGYDEINLNCGCPSERVQRGAFGACLMKEPELVADCVKAMRDAVSVPVTVKHRIGVDDVEDYAFVRDFVGTIAD
AGCDVFIVHARNAILKGLSPKENREIPPLKYDYAYRLKRDFPQLEIIINGGIKTLDEVETHLQHVDGVMLGREAYHNPFV
LADVDARFYGSTDAAPTREEAEAQLIEYCRAELARGTYLGAIVRHALGLYRGVAGARGWRRVLSDNKKLAARDLAIFDEA
RQHLREPSEMFE

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=236, Percent_Identity=29.2372881355932, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI40807366, Length=244, Percent_Identity=28.6885245901639, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI145693211, Length=312, Percent_Identity=60.2564102564103, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1789660, Length=240, Percent_Identity=24.1666666666667, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788462, Length=168, Percent_Identity=32.1428571428571, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=255, Percent_Identity=24.7058823529412, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=236, Percent_Identity=26.6949152542373, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24580595, Length=295, Percent_Identity=25.0847457627119, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19920448, Length=295, Percent_Identity=25.0847457627119, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19921524, Length=235, Percent_Identity=27.6595744680851, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37254; Mature: 37123

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKPNVSARRVSVAPMMDWTDRHCRSFHRTLSRHAWLYTEMVTTGALIHGDVARHLAFT
CCCCCCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHC
ADEAPVALQLGGSEREDLARSAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMKEPELV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCHHHCCCHHHH
ADCVKAMRDAVSVPVTVKHRIGVDDVEDYAFVRDFVGTIADAGCDVFIVHARNAILKGLS
HHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCC
PKENREIPPLKYDYAYRLKRDFPQLEIIINGGIKTLDEVETHLQHVDGVMLGREAYHNPF
CCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHCCCE
VLADVDARFYGSTDAAPTREEAEAQLIEYCRAELARGTYLGAIVRHALGLYRGVAGARGW
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RRVLSDNKKLAARDLAIFDEARQHLREPSEMFE
HHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
SSKPNVSARRVSVAPMMDWTDRHCRSFHRTLSRHAWLYTEMVTTGALIHGDVARHLAFT
CCCCCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHC
ADEAPVALQLGGSEREDLARSAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMKEPELV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCHHHCCCHHHH
ADCVKAMRDAVSVPVTVKHRIGVDDVEDYAFVRDFVGTIADAGCDVFIVHARNAILKGLS
HHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCC
PKENREIPPLKYDYAYRLKRDFPQLEIIINGGIKTLDEVETHLQHVDGVMLGREAYHNPF
CCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHCCCE
VLADVDARFYGSTDAAPTREEAEAQLIEYCRAELARGTYLGAIVRHALGLYRGVAGARGW
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RRVLSDNKKLAARDLAIFDEARQHLREPSEMFE
HHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]