Click here to switch to the map view.

The map label for this gene is lexA

Identifier: 186476388

GI number: 186476388

Start: 1853111

End: 1853761

Strand: Direct

Name: lexA

Synonym: Bphy_1630

Alternate gene names: 186476388

Gene position: 1853111-1853761 (Clockwise)

Preceding gene: 186476380

Following gene: 186476389

Centisome position: 53.26

GC content: 63.75

Gene sequence:

>651_bases
ATGACCAAACTTACCGCACGTCAGCAACAGGTTTTCGATCTGATCCGCCGCGCCATCGAGCGCACGGGCTTCCCGCCCAC
GCGGGCCGAGATCGCGGCCGAACTCGGCTTCAGTTCGGCCAACTCGGCGGAAGAGCATCTGCGGGCGCTGGCGCGCAAAG
GTGTCATCGAACTGGCAGCGGGCGCGTCGCGCGGCATCCGGCTCATCGCCGGTCAGGACGACTTGCCGCATCAGTTCACG
CTGCCGCACGCGAGCATCATGCAGTTGTCGCTGCCGCTCGTCGGCCGTGTGGCCGCGGGCAGTCCGATCCTCGCGCAAGA
ACATATCTCGCAAACGTATATGTGCGACCCGTCGCTGTTCTCGAGCAAGCCCGACTACCTGTTGAAGGTGCGCGGCCTGT
CGATGCGCGACGCGGGCATCTTCGACGGCGACCTGCTCGCCGTGCAGAAAAAGAGCGAAGCGAAAGACGGCCAGATCATC
GTTGCGCGTCTGGGCGACGACGTGACCGTGAAGCGTCTGAAGCGGCGGCCTGATGGGCTCGAGCTGATCGCCGAGAACCC
CGATTACGAGAATATTTTTGTTCAGGCCGGCAGTGCGGAGTTCGCGCTCGAAGGCATCGCGGTCGGTCTGATCCGGCCGT
CGGAATTCTAA

Upstream 100 bases:

>100_bases
CGTCCTCGCGCACCGCACAGCCAAGAACTTAAAGTAAAGCTTGAAATGCGCCGGATACTGTATAAAAATACAGGCACCTG
TCTATCCATACAGTGGCGCC

Downstream 100 bases:

>100_bases
TTAGCTGAACACTGTCTGGAGAGACTCATGGAACGCTTTGCCCGCTTGCTGCCCTTTCGCCAGTTCGGTCGTCTGCGCCA
TCTGCGCAACCTCGTCCCGT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MTKLTARQQQVFDLIRRAIERTGFPPTRAEIAAELGFSSANSAEEHLRALARKGVIELAAGASRGIRLIAGQDDLPHQFT
LPHASIMQLSLPLVGRVAAGSPILAQEHISQTYMCDPSLFSSKPDYLLKVRGLSMRDAGIFDGDLLAVQKKSEAKDGQII
VARLGDDVTVKRLKRRPDGLELIAENPDYENIFVQAGSAEFALEGIAVGLIRPSEF

Sequences:

>Translated_216_residues
MTKLTARQQQVFDLIRRAIERTGFPPTRAEIAAELGFSSANSAEEHLRALARKGVIELAAGASRGIRLIAGQDDLPHQFT
LPHASIMQLSLPLVGRVAAGSPILAQEHISQTYMCDPSLFSSKPDYLLKVRGLSMRDAGIFDGDLLAVQKKSEAKDGQII
VARLGDDVTVKRLKRRPDGLELIAENPDYENIFVQAGSAEFALEGIAVGLIRPSEF
>Mature_215_residues
TKLTARQQQVFDLIRRAIERTGFPPTRAEIAAELGFSSANSAEEHLRALARKGVIELAAGASRGIRLIAGQDDLPHQFTL
PHASIMQLSLPLVGRVAAGSPILAQEHISQTYMCDPSLFSSKPDYLLKVRGLSMRDAGIFDGDLLAVQKKSEAKDGQIIV
ARLGDDVTVKRLKRRPDGLELIAENPDYENIFVQAGSAEFALEGIAVGLIRPSEF

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=216, Percent_Identity=54.6296296296296, Blast_Score=236, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_BURP8 (B2JK89)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001857858.1
- ProteinModelPortal:   B2JK89
- MEROPS:   S24.001
- GeneID:   6243121
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_1630
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- ProtClustDB:   PRK00215
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 23503; Mature: 23372

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: ACT_SITE 134-134 ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLTARQQQVFDLIRRAIERTGFPPTRAEIAAELGFSSANSAEEHLRALARKGVIELAA
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEC
GASRGIRLIAGQDDLPHQFTLPHASIMQLSLPLVGRVAAGSPILAQEHISQTYMCDPSLF
CCCCCEEEEECCCCCCCEEECCCHHHHHHHCCHHHHHCCCCCCHHHHHCCHHEEECCHHH
SSKPDYLLKVRGLSMRDAGIFDGDLLAVQKKSEAKDGQIIVARLGDDVTVKRLKRRPDGL
CCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHCCCCCE
ELIAENPDYENIFVQAGSAEFALEGIAVGLIRPSEF
EEEECCCCCCEEEEECCCCCEEECCEEEEEECCCCC
>Mature Secondary Structure 
TKLTARQQQVFDLIRRAIERTGFPPTRAEIAAELGFSSANSAEEHLRALARKGVIELAA
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEC
GASRGIRLIAGQDDLPHQFTLPHASIMQLSLPLVGRVAAGSPILAQEHISQTYMCDPSLF
CCCCCEEEEECCCCCCCEEECCCHHHHHHHCCHHHHHCCCCCCHHHHHCCHHEEECCHHH
SSKPDYLLKVRGLSMRDAGIFDGDLLAVQKKSEAKDGQIIVARLGDDVTVKRLKRRPDGL
CCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHCCCCCE
ELIAENPDYENIFVQAGSAEFALEGIAVGLIRPSEF
EEEECCCCCCEEEEECCCCCEEECCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA