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The map label for this gene is gcl [H]

Identifier: 186476378

GI number: 186476378

Start: 1841045

End: 1842820

Strand: Direct

Name: gcl [H]

Synonym: Bphy_1620

Alternate gene names: 186476378

Gene position: 1841045-1842820 (Clockwise)

Preceding gene: 186476376

Following gene: 186476379

Centisome position: 52.92

GC content: 63.46

Gene sequence:

>1776_bases
ATGGCCAAGATGAGAGCCGTCGACGCAGCGGTGCTCGTGCTCGAAAAAGAAGGCATCGACACGGCTTTCGGCGTTCCCGG
CGCCGCCATCAACCCGTTCTACTCGGCCATGCGCAAGGCGGGCAACATCAGCCACGTGCTCGCACGCCATGTCGAAGGGG
CGTCGCACATGGCCGAAGGCTACACGCGTGCTGCGCCGGGCAATATTGGGGTGTGCATTGGCACGTCGGGCCCTGCGGGC
ACCGACATGATCACGGGCCTCTACTCCGCTTCCGCCGATTCGATCCCGATTCTTGCGATCACGGGCCAGGCGCCGCGCGC
GCGCCTGTACAAGGAAGACTTCCAGGCTGTCGATATCGAATCGATCGCGAAGCCCGTCACGAAGTGGGCCGTCACGGTTC
GCGAGCCGGCCCTCGTGCCGCGCGTGTTCCAGCAGGCCTTCCATCTGATGCGCTCGGGCCGCCCTGGCCCCGTGCTGGTC
GACCTGCCCATCGACGTGCAACTCGCCGAAATCGAATTCGACATCGACACCTACGAACCGCTGCCCGTCTACAAGCCGAA
AGCGACGCGCAAGCAGATCGAAGCGGCGCTCACGCTGCTCAACGATTCGGCGAAGCCGTTGATCGTCTCCGGCGGCGGCG
TGCTCAACGCTGCCGCTGAAGACCTGCTCGTGCGGTTTGCCGAAACGATCGGCGTGCCCGTGATCCCGACGCTGATGTCG
TGGGGCGCGATTCCCGACGATCACCCGCTGATGGCGGGCATGGTCGGCCTGCAGACGTCACATCGTTACGGCAACGCGAC
GATGCTCGCGTCCGACTTCGTGCTCGGCATCGGTAATCGCTGGGCGAACCGGCACACGGGCAGCGTCGAGGTCTATACGA
AGGGCCGCAAGTTCGTGCACGTCGATATCGAACCGACACAGATCGGCCGTGTGTTCGGTCCGGATCTCGGCATCGTGTCC
GACGCGAAGGCCGCGATCGAACTGTTCATTGAAGTTGCGCAGGAATGGAAGGCGGCTGGCAAGCTGAAGGACCGCAGCGC
ATGGGTCGAGGAATGCCAGGCTCGCAAGCGCACGCTGCAGCGCAAGACGCACTTCGAAAACGTGCCGATCAAGCCGCAGC
GCGTGTACGAAGAAATGAACAAGGTGTTCGGCCGCGATACATGCTACGTGAGCACGATCGGCCTGTCGCAGATCGCTGCC
GCGCAGTTCCTGCACGTGTTCAAGGCGCGCAACTGGATCAACTGCGGCCAGGCAGGCCCGCTCGGCTGGACGATTCCGGC
CGCGCTCGGCGTGCGTGCTGCCGATCCGCAGCGCCCCATCGTCGCGCTGTCGGGCGACTACGACTTCCAGTTCATGATCG
AAGAACTGGCCGTGGGCGCACAGTTCAAGCTGCCGTACGTGCATGTCGTCGTGAACAACTCGTATCTGGGCCTGATCCGC
CAGGCACAGCGCGCGTTCGACATGGACTACTGCGTGCAGCTCGCGTTCGACAACATCAACGCACCGGAAGTCGAAGGTTA
TGGCGTCGATCACGTGGCAGTGGCGGAAGGGCTCGGCTGCAAGGCGATCCGCGTGTTCAAGCCGGAAGACATCCAGCCCG
CACTGCAAAAGGCGCAGTCGATGCTCTCCGAGTTCAACGTGCCCGTGATCGTCGAAGTGATCCTCGAGCGTGTGACGAAC
ATTTCGATGGGCACCGAGATCGACGCGATCAACGAGTTCGAAGAGCTCGCCATCACGCGCGCCGATGCACCGAGCGCAGT
CACGCTGCTCGACTGA

Upstream 100 bases:

>100_bases
GTTTTGAAATAAGCGACCGTGGATCATCTTATCAAACCTTAGATGCAGTCTTAAAGTCTGTCCAACACCCATCCGCTTTA
CAGACTTCCAGGAGACATTC

Downstream 100 bases:

>100_bases
TCTCCGCCGTATCCGCTTCACCGGGATGGGTGCGCTTCGCACGAAGCGCATTCGTCCGCGTCACGCTGTTCACGCCGTTC
AATTTGACCGACCAGAGAGA

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MAKMRAVDAAVLVLEKEGIDTAFGVPGAAINPFYSAMRKAGNISHVLARHVEGASHMAEGYTRAAPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLV
DLPIDVQLAEIEFDIDTYEPLPVYKPKATRKQIEAALTLLNDSAKPLIVSGGGVLNAAAEDLLVRFAETIGVPVIPTLMS
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAAIELFIEVAQEWKAAGKLKDRSAWVEECQARKRTLQRKTHFENVPIKPQRVYEEMNKVFGRDTCYVSTIGLSQIAA
AQFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAVGAQFKLPYVHVVVNNSYLGLIR
QAQRAFDMDYCVQLAFDNINAPEVEGYGVDHVAVAEGLGCKAIRVFKPEDIQPALQKAQSMLSEFNVPVIVEVILERVTN
ISMGTEIDAINEFEELAITRADAPSAVTLLD

Sequences:

>Translated_591_residues
MAKMRAVDAAVLVLEKEGIDTAFGVPGAAINPFYSAMRKAGNISHVLARHVEGASHMAEGYTRAAPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLV
DLPIDVQLAEIEFDIDTYEPLPVYKPKATRKQIEAALTLLNDSAKPLIVSGGGVLNAAAEDLLVRFAETIGVPVIPTLMS
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAAIELFIEVAQEWKAAGKLKDRSAWVEECQARKRTLQRKTHFENVPIKPQRVYEEMNKVFGRDTCYVSTIGLSQIAA
AQFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAVGAQFKLPYVHVVVNNSYLGLIR
QAQRAFDMDYCVQLAFDNINAPEVEGYGVDHVAVAEGLGCKAIRVFKPEDIQPALQKAQSMLSEFNVPVIVEVILERVTN
ISMGTEIDAINEFEELAITRADAPSAVTLLD
>Mature_590_residues
AKMRAVDAAVLVLEKEGIDTAFGVPGAAINPFYSAMRKAGNISHVLARHVEGASHMAEGYTRAAPGNIGVCIGTSGPAGT
DMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLVD
LPIDVQLAEIEFDIDTYEPLPVYKPKATRKQIEAALTLLNDSAKPLIVSGGGVLNAAAEDLLVRFAETIGVPVIPTLMSW
GAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVSD
AKAAIELFIEVAQEWKAAGKLKDRSAWVEECQARKRTLQRKTHFENVPIKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAA
QFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAVGAQFKLPYVHVVVNNSYLGLIRQ
AQRAFDMDYCVQLAFDNINAPEVEGYGVDHVAVAEGLGCKAIRVFKPEDIQPALQKAQSMLSEFNVPVIVEVILERVTNI
SMGTEIDAINEFEELAITRADAPSAVTLLD

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=23.0357142857143, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI21361361, Length=597, Percent_Identity=23.6180904522613, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1786717, Length=590, Percent_Identity=77.9661016949153, Blast_Score=972, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=33.5701598579041, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI1790104, Length=476, Percent_Identity=31.0924369747899, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=25.9528130671506, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788716, Length=477, Percent_Identity=25.1572327044025, Blast_Score=129, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17542570, Length=545, Percent_Identity=24.5871559633028, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17531299, Length=553, Percent_Identity=22.2423146473779, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17531301, Length=553, Percent_Identity=22.2423146473779, Blast_Score=93, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=570, Percent_Identity=30.1754385964912, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6320816, Length=564, Percent_Identity=24.468085106383, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321524, Length=492, Percent_Identity=21.7479674796748, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323163, Length=496, Percent_Identity=21.5725806451613, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922626, Length=576, Percent_Identity=25.1736111111111, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64254; Mature: 64122

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRAVDAAVLVLEKEGIDTAFGVPGAAINPFYSAMRKAGNISHVLARHVEGASHMAEG
CCCCHHCCEEEEEEEECCCCHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHC
YTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLVDLPIDVQLAEIEFDIDTYEP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCC
LPVYKPKATRKQIEAALTLLNDSAKPLIVSGGGVLNAAAEDLLVRFAETIGVPVIPTLMS
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAAIELFIEVAQEWKAAGKLKDRSAWVEECQARKRTLQ
EECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RKTHFENVPIKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAAQFLHVFKARNWINCGQAGP
HHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAVGAQFKLPYVHVVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEECCCEEEEEECCCHHHHHH
QAQRAFDMDYCVQLAFDNINAPEVEGYGVDHVAVAEGLGCKAIRVFKPEDIQPALQKAQS
HHHHHHCHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHH
MLSEFNVPVIVEVILERVTNISMGTEIDAINEFEELAITRADAPSAVTLLD
HHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEECCCCCCEEEECC
>Mature Secondary Structure 
AKMRAVDAAVLVLEKEGIDTAFGVPGAAINPFYSAMRKAGNISHVLARHVEGASHMAEG
CCCHHCCEEEEEEEECCCCHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHC
YTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLVDLPIDVQLAEIEFDIDTYEP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCC
LPVYKPKATRKQIEAALTLLNDSAKPLIVSGGGVLNAAAEDLLVRFAETIGVPVIPTLMS
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAAIELFIEVAQEWKAAGKLKDRSAWVEECQARKRTLQ
EECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RKTHFENVPIKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAAQFLHVFKARNWINCGQAGP
HHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAVGAQFKLPYVHVVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEECCCEEEEEECCCHHHHHH
QAQRAFDMDYCVQLAFDNINAPEVEGYGVDHVAVAEGLGCKAIRVFKPEDIQPALQKAQS
HHHHHHCHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHH
MLSEFNVPVIVEVILERVTNISMGTEIDAINEFEELAITRADAPSAVTLLD
HHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]