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The map label for this gene is mdeA [H]

Identifier: 186476330

GI number: 186476330

Start: 1781979

End: 1783313

Strand: Direct

Name: mdeA [H]

Synonym: Bphy_1572

Alternate gene names: 186476330

Gene position: 1781979-1783313 (Clockwise)

Preceding gene: 186476325

Following gene: 186476331

Centisome position: 51.22

GC content: 67.79

Gene sequence:

>1335_bases
ATGCCCGCCAACCGTTTCGACACACTCGCGCTGCACGCGGGCGCCGCCCCCGATCCTGCAACGGGCGCGCGCGCGACGCC
CATCTACCAGACCACCTCGTTCACGTTCCGCGATACCGACCACGCTGCCGCACTCTTCAACATGGAACGCGCCGGCCACG
TCTATTCGCGGATCTCGAACCCGACCGTCGCCGTGTTCGAAGAGCGCGTCGCCGCGCTCGAAAACGGCGCGGGCGCGATC
GGCACGGCAAGCGGCCAGGCCGCGCTGCATCTGGCGATTGCGACGCTGATGGGCGCGGGCTCGCATATCGTCGCGTCGAG
CGCGCTGTATGGCGGCTCGCACAATCTGCTCAACTACACGCTGCGGCGCTTCGGTATCGAGACGACCTTCGTCAAACCCG
GCGACATTGACGCATGGCGCGCGGCGCTGCGCCCGAACACGAAGCTCCTGTTCGGCGAGACGCTCGGCAATCCCGGCCTC
GATGTGCTCGATAGCGAGACAGTCGCCCAGATCGCGCACGACCACCGCGTGCCGCTGCTGGTCGATTCCACTTTCACCAC
GCCGTACCTGCTCAAGCCGTTCGACCACGGCGCCGACTTCGTCTACCACTCCGCGACCAAATTCCTCGGCGGTCACGGCA
CGACGATCGGCGGCGTGCTCGTCGACGGCGGCACCTTCGACTTCGAAGCGTCCGGGCGCTTCCCCGAACTCACCGAGCCC
TATGAGGGCTTTCACGGCATGGTGTTCGCCGAGGAAAGCACCGTCGCGCCGTTCCTGTTGCGCGCCCGGCGCGAAGGACT
CCGCGACTTCGGCGCGTGCCTGCATCCGCAAGCCGCGTGGCAGCTGCTTCAGGGCGTCGAGACGCTGCCGCTACGCATGG
AACGCCATGTCGCGAATACGCGCAAGGTGGTCGAATTCCTCGCCGCGCATGCCGCCGTCGAATCGGTCGCGTACCCGGAG
CTGCCGACGCACCCCGATCACGCGCTCGCCCAGCGCCTGCTGCCGCGCGGCGCAGGCGCCGTGTTCAGTTTCGATCTGCG
CGGGGACCGTGCCGCCGGCCGCGCGTTCATCGAAGCGCTGTCGCTGTTCTCGCATCTCGCGAATGTCGGCGATGCGCGCT
CGCTCGTCATTCATCCTGCATCGACGACGCACTTTCGCATGGATGCCGCCGCGCTCGCCATGGCGGGCATCGCGGAAGGC
ACCATACGGCTGTCGATCGGCCTCGAAGACCCGGACGACCTGATCGACGATCTCAAGCGCGCGCTCAAGGCCGCGCAAAA
ATCCGGCGAGCGTTCCACGCCGTCACCCGCCGCCCCGCGCAAGGAGTCCGCATGA

Upstream 100 bases:

>100_bases
GCCGCACTTCGGGCGACAACCGAGTGGTTCGCGCGCGGCACGGTGATACGATGGCCTCCACGCTCACCTTCGCCGGGCTG
TCGAGGCCCGGCCGCCCACG

Downstream 100 bases:

>100_bases
TCGTGGACGTCAACGGCAAATCCGCCTATGTCTATACGGGCGGCAAGGCGTTCGACGCCGCGTTGCCCACCGCCGTCTTC
ATTCATGGCGCCGAGCATGA

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MPANRFDTLALHAGAAPDPATGARATPIYQTTSFTFRDTDHAAALFNMERAGHVYSRISNPTVAVFEERVAALENGAGAI
GTASGQAALHLAIATLMGAGSHIVASSALYGGSHNLLNYTLRRFGIETTFVKPGDIDAWRAALRPNTKLLFGETLGNPGL
DVLDSETVAQIAHDHRVPLLVDSTFTTPYLLKPFDHGADFVYHSATKFLGGHGTTIGGVLVDGGTFDFEASGRFPELTEP
YEGFHGMVFAEESTVAPFLLRARREGLRDFGACLHPQAAWQLLQGVETLPLRMERHVANTRKVVEFLAAHAAVESVAYPE
LPTHPDHALAQRLLPRGAGAVFSFDLRGDRAAGRAFIEALSLFSHLANVGDARSLVIHPASTTHFRMDAAALAMAGIAEG
TIRLSIGLEDPDDLIDDLKRALKAAQKSGERSTPSPAAPRKESA

Sequences:

>Translated_444_residues
MPANRFDTLALHAGAAPDPATGARATPIYQTTSFTFRDTDHAAALFNMERAGHVYSRISNPTVAVFEERVAALENGAGAI
GTASGQAALHLAIATLMGAGSHIVASSALYGGSHNLLNYTLRRFGIETTFVKPGDIDAWRAALRPNTKLLFGETLGNPGL
DVLDSETVAQIAHDHRVPLLVDSTFTTPYLLKPFDHGADFVYHSATKFLGGHGTTIGGVLVDGGTFDFEASGRFPELTEP
YEGFHGMVFAEESTVAPFLLRARREGLRDFGACLHPQAAWQLLQGVETLPLRMERHVANTRKVVEFLAAHAAVESVAYPE
LPTHPDHALAQRLLPRGAGAVFSFDLRGDRAAGRAFIEALSLFSHLANVGDARSLVIHPASTTHFRMDAAALAMAGIAEG
TIRLSIGLEDPDDLIDDLKRALKAAQKSGERSTPSPAAPRKESA
>Mature_443_residues
PANRFDTLALHAGAAPDPATGARATPIYQTTSFTFRDTDHAAALFNMERAGHVYSRISNPTVAVFEERVAALENGAGAIG
TASGQAALHLAIATLMGAGSHIVASSALYGGSHNLLNYTLRRFGIETTFVKPGDIDAWRAALRPNTKLLFGETLGNPGLD
VLDSETVAQIAHDHRVPLLVDSTFTTPYLLKPFDHGADFVYHSATKFLGGHGTTIGGVLVDGGTFDFEASGRFPELTEPY
EGFHGMVFAEESTVAPFLLRARREGLRDFGACLHPQAAWQLLQGVETLPLRMERHVANTRKVVEFLAAHAAVESVAYPEL
PTHPDHALAQRLLPRGAGAVFSFDLRGDRAAGRAFIEALSLFSHLANVGDARSLVIHPASTTHFRMDAAALAMAGIAEGT
IRLSIGLEDPDDLIDDLKRALKAAQKSGERSTPSPAAPRKESA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=424, Percent_Identity=33.2547169811321, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI299473759, Length=422, Percent_Identity=30.8056872037915, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI25453487, Length=421, Percent_Identity=28.978622327791, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=33.3333333333333, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1789383, Length=429, Percent_Identity=26.8065268065268, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71980708, Length=405, Percent_Identity=32.0987654320988, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17538055, Length=424, Percent_Identity=32.311320754717, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17560056, Length=423, Percent_Identity=31.2056737588652, Blast_Score=184, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=444, Percent_Identity=42.5675675675676, Blast_Score=306, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6319307, Length=430, Percent_Identity=33.7209302325581, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321254, Length=450, Percent_Identity=25.7777777777778, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321170, Length=354, Percent_Identity=24.8587570621469, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=427, Percent_Identity=27.1662763466042, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19922554, Length=425, Percent_Identity=33.6470588235294, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47441; Mature: 47310

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPANRFDTLALHAGAAPDPATGARATPIYQTTSFTFRDTDHAAALFNMERAGHVYSRISN
CCCCCCCEEEEECCCCCCCCCCCCCCCEEEECEEEECCCCHHHHHHHHHHHHHHHHHCCC
PTVAVFEERVAALENGAGAIGTASGQAALHLAIATLMGAGSHIVASSALYGGSHNLLNYT
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
LRRFGIETTFVKPGDIDAWRAALRPNTKLLFGETLGNPGLDVLDSETVAQIAHDHRVPLL
HHHHCCEEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEE
VDSTFTTPYLLKPFDHGADFVYHSATKFLGGHGTTIGGVLVDGGTFDFEASGRFPELTEP
EECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCEECEEEECCCEEEEECCCCCCCCCCC
YEGFHGMVFAEESTVAPFLLRARREGLRDFGACLHPQAAWQLLQGVETLPLRMERHVANT
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHH
RKVVEFLAAHAAVESVAYPELPTHPDHALAQRLLPRGAGAVFSFDLRGDRAAGRAFIEAL
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
SLFSHLANVGDARSLVIHPASTTHFRMDAAALAMAGIAEGTIRLSIGLEDPDDLIDDLKR
HHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHH
ALKAAQKSGERSTPSPAAPRKESA
HHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PANRFDTLALHAGAAPDPATGARATPIYQTTSFTFRDTDHAAALFNMERAGHVYSRISN
CCCCCCEEEEECCCCCCCCCCCCCCCEEEECEEEECCCCHHHHHHHHHHHHHHHHHCCC
PTVAVFEERVAALENGAGAIGTASGQAALHLAIATLMGAGSHIVASSALYGGSHNLLNYT
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
LRRFGIETTFVKPGDIDAWRAALRPNTKLLFGETLGNPGLDVLDSETVAQIAHDHRVPLL
HHHHCCEEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEE
VDSTFTTPYLLKPFDHGADFVYHSATKFLGGHGTTIGGVLVDGGTFDFEASGRFPELTEP
EECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCEECEEEECCCEEEEECCCCCCCCCCC
YEGFHGMVFAEESTVAPFLLRARREGLRDFGACLHPQAAWQLLQGVETLPLRMERHVANT
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHH
RKVVEFLAAHAAVESVAYPELPTHPDHALAQRLLPRGAGAVFSFDLRGDRAAGRAFIEAL
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
SLFSHLANVGDARSLVIHPASTTHFRMDAAALAMAGIAEGTIRLSIGLEDPDDLIDDLKR
HHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHH
ALKAAQKSGERSTPSPAAPRKESA
HHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]