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The map label for this gene is lpsJ [H]

Identifier: 186476320

GI number: 186476320

Start: 1770678

End: 1771319

Strand: Reverse

Name: lpsJ [H]

Synonym: Bphy_1562

Alternate gene names: 186476320

Gene position: 1771319-1770678 (Counterclockwise)

Preceding gene: 186476321

Following gene: 186476319

Centisome position: 50.91

GC content: 63.08

Gene sequence:

>642_bases
ATGGCTTGGAATCGTGACCAGATGGCCGCGCGCGCGGCGCAGGAACTGCAAGACGGCTTCTACGTGAACCTCGGCATCGG
CCTGCCGACGCTCGTCGCCAACCACGTGCCCGCAGGCATGGAAGTGTGGCTGCAATCGGAAAACGGCCTGCTCGGCATCG
GCCCATCGCCCTATGAAGACGAAGTCGACCCGGACCTCATCAACGCCGGCAAGCAGACTGTGACGACGCTGCCCGGTTCG
TCGATTTTCTCGTCGGCCGATTCGTTCGCGATGATTCGCGGCGGCCACATCAATCTTGCCATTCTGGGCGCGATGCAGAT
CAGCAAGAAGGGCGATCTGGCGAACTGGATGATCCCCGGCAAGATGATCAAGGGCATGGGCGGCGCGATGGATCTCGTTG
CCGGCGTGAAGAGAGTCGTCGTGCTGATGGAGCACGTCGCGAAGGGCGACCAGCACAAGATCCTCGAAGAATGCACGCTG
CCGCTGACGGGCGTGGGCGTGGTCGATCGCATCATCACCGACCTCGGTGTGATCGACGTCGTCGACGGCGGCCTGAAGCT
GGTCGAACTGGCTGACGGCGTCACGGTCGATGAGATCAGGGCGAAGACGGGCGCGCCGCTCGACACGAGCGCCGTGCAAT
AA

Upstream 100 bases:

>100_bases
CGATCCACACGCCGGGCATCTTCGTGCAGCGCCTCGTGCTGAATGCCCACCCGGAAAAACGCATTGAACAACGCATCGTC
CGCGCGAAAGGAGAGTAACC

Downstream 100 bases:

>100_bases
GGTCTCACCCGGTCTTGCGCCGGGTGCGGTTGCCGCAAAGCGGGCGCGACGCAGGTCGCGCCCGCTCTTCTTTTGGGCGC
TCCTCTTTAACGTTTCGTGC

Product: 3-oxoacid CoA-transferase subunit B

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MAWNRDQMAARAAQELQDGFYVNLGIGLPTLVANHVPAGMEVWLQSENGLLGIGPSPYEDEVDPDLINAGKQTVTTLPGS
SIFSSADSFAMIRGGHINLAILGAMQISKKGDLANWMIPGKMIKGMGGAMDLVAGVKRVVVLMEHVAKGDQHKILEECTL
PLTGVGVVDRIITDLGVIDVVDGGLKLVELADGVTVDEIRAKTGAPLDTSAVQ

Sequences:

>Translated_213_residues
MAWNRDQMAARAAQELQDGFYVNLGIGLPTLVANHVPAGMEVWLQSENGLLGIGPSPYEDEVDPDLINAGKQTVTTLPGS
SIFSSADSFAMIRGGHINLAILGAMQISKKGDLANWMIPGKMIKGMGGAMDLVAGVKRVVVLMEHVAKGDQHKILEECTL
PLTGVGVVDRIITDLGVIDVVDGGLKLVELADGVTVDEIRAKTGAPLDTSAVQ
>Mature_212_residues
AWNRDQMAARAAQELQDGFYVNLGIGLPTLVANHVPAGMEVWLQSENGLLGIGPSPYEDEVDPDLINAGKQTVTTLPGSS
IFSSADSFAMIRGGHINLAILGAMQISKKGDLANWMIPGKMIKGMGGAMDLVAGVKRVVVLMEHVAKGDQHKILEECTLP
LTGVGVVDRIITDLGVIDVVDGGLKLVELADGVTVDEIRAKTGAPLDTSAVQ

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=208, Percent_Identity=61.0576923076923, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI11545841, Length=208, Percent_Identity=61.0576923076923, Blast_Score=249, Evalue=2e-66,
Organism=Escherichia coli, GI1788552, Length=207, Percent_Identity=51.207729468599, Blast_Score=220, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI17531517, Length=209, Percent_Identity=56.4593301435407, Blast_Score=238, Evalue=2e-63,
Organism=Drosophila melanogaster, GI21358325, Length=208, Percent_Identity=59.6153846153846, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24655794, Length=208, Percent_Identity=59.6153846153846, Blast_Score=254, Evalue=3e-68,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 22425; Mature: 22294

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWNRDQMAARAAQELQDGFYVNLGIGLPTLVANHVPAGMEVWLQSENGLLGIGPSPYED
CCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCC
EVDPDLINAGKQTVTTLPGSSIFSSADSFAMIRGGHINLAILGAMQISKKGDLANWMIPG
CCCHHHHHCCCCEEEECCCCHHHHCCCCEEEEECCCEEEEEEEEEEECCCCCCCCEECCH
KMIKGMGGAMDLVAGVKRVVVLMEHVAKGDQHKILEECTLPLTGVGVVDRIITDLGVIDV
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHCCHHEE
VDGGLKLVELADGVTVDEIRAKTGAPLDTSAVQ
CCCCEEEEHHCCCCCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AWNRDQMAARAAQELQDGFYVNLGIGLPTLVANHVPAGMEVWLQSENGLLGIGPSPYED
CCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCC
EVDPDLINAGKQTVTTLPGSSIFSSADSFAMIRGGHINLAILGAMQISKKGDLANWMIPG
CCCHHHHHCCCCEEEECCCCHHHHCCCCEEEEECCCEEEEEEEEEEECCCCCCCCEECCH
KMIKGMGGAMDLVAGVKRVVVLMEHVAKGDQHKILEECTLPLTGVGVVDRIITDLGVIDV
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHCCHHEE
VDGGLKLVELADGVTVDEIRAKTGAPLDTSAVQ
CCCCEEEEHHCCCCCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]