Click here to switch to the map view.

The map label for this gene is bclA [H]

Identifier: 186476301

GI number: 186476301

Start: 1743823

End: 1745421

Strand: Direct

Name: bclA [H]

Synonym: Bphy_1543

Alternate gene names: 186476301

Gene position: 1743823-1745421 (Clockwise)

Preceding gene: 186476300

Following gene: 186476302

Centisome position: 50.12

GC content: 61.66

Gene sequence:

>1599_bases
ATGCAAGCCTTGCCGGAATCGCCGCCACGCGAGCCTGCACCGACAGTCGAGCCGCCGCCCGCGCAGTTCAACTTCGCGTC
CCATCTGTTTCGGCTGAACGACGTGCGGGCAGACAAGCCCGCCTACATCGACGATGCAGGCGTCACGTCGTATGCGCAAC
TCGAGCACCGCTCGCGCCGCTTCGCTTCCGCTTTGCGCTCGCTGGGTGTGCATGCCGAAGAACGGATCCTGCTCGTGATG
CTCGACACGGCGGAATTGCCCGTCGCGTTTCTCGGCGCGCTGTATGCGGGTGTCGTGCCCGTCGTGGCGAACACGCTGCT
GACGGCGACCGACTATCGGTACATGCTCACGCACAGCCACGCACGCGCGGTGATCGCTTCGGGTCCACTGGTGCCGACGG
TCGAGCAAGCGTTGAGCGAAGCGGAACAAGACGGCTGCCTGCTGATCGTGTCGCAGCCGGTGCACTTCGATCCGCCGCCG
ATGCATGTGCTGAGCCGGCTGATCGACGGTGCCGAGCCTCTGCTGAAACCGAATGCAAGTAGCGGCGACGATATTGCATT
CTGGTTGTATTCATCGGGTTCCACGGGCAAGCCGAAAGGCACCGTCCATACGCACGCGAACCTGTACTGGACTGCAGAGC
TTTACGCGAAGCCGATACTCGGCATCAATGAGCGCGACGTCGTGTTCTCCGCAGCCAAGCTGTTCTTTGCCTATGGGCTC
GGCAACGCGCTGACATTCCCATTGACGGTCGGCGCAACGACGGTATTGATGGCCGAACGTCCGACACCGGACGCGGTTTT
CAGGCGGCTCGTCGAGCATCGGCCGACGATCTTCTACGGCGTACCGACGCTGTACGCGAGCATGCTGGTGTCGCCGAACC
TGCCGCCGCGCGAGCAGGTGGCGATGCGCATCTGTGCGTCGGCGGGCGAAGCATTGCCACGAGAAATCGGCGAACGGTTC
ACCGCGCATTTCGGATGTGAGATTCTCGACGGCATCGGTTCGACCGAAATGCTGCATATCTTCCTGTCGAACCGCGCAGG
CGAAGTCGAGTACGGAACGACGGGCCGTCCGGTGCCTGGATATGAAGTGGAGTTGCGCGATGAAGCGGGTCGCACGGTGC
CGGACGGCGAGGTCGGCGATCTGTTCATCAAGGGACCCAGCGCCGCCCTGATGTACTGGAGCAACCGCGAGAAATCGCGA
GCGACCTTTCTGGGCGAATGGATTCGTAGTGGCGACAAGTATTGCCGCCTGCCGAACGGCTGCTATCAATACGCAGGGCG
CAGCGACGACATGTTGAAGGTAAGCGGCCAGTATGTGTCGCCCGTCGAAGTGGAAATGGCGCTCGTGCAACATACTGCCG
TGCTGGAGGCCGCGGTTGTCGGCGTCGATCATGGCGGTCTCGTGAAAACGCGTGCGTTCGTCGTGCTGAAGCAAACTGCC
GATGCATGCGACACACTCGCCGATGACCTGAAGTCGTTCGTCAAGGGCAAGCTCGCGCCGCACAAGTATCCTCGCGATAT
CGTCTTCGTCGACGATCTGCCGAAGACGGCAACGGGTAAGATTCAACGCTTCAAATTGCGAGAACAGCTCACTAGCTGA

Upstream 100 bases:

>100_bases
CGAATTCACACGGGACGCGACCACTGTGTGACGTCACGGCGCGTATCGCTCTCATCCTGCATTTCCGTTCCACGCAGACC
GCCACTTCGCCGGAGGACAC

Downstream 100 bases:

>100_bases
AGAACGGAATGGCCGAACCTGTGACCACCTTGCGCGATGCGTCCCAGAGCGCATCGATTGCATTGCCGGCGAACGACCAA
CGCGGCGCGTTGACGATCGA

Product: benzoate-CoA ligase family

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MQALPESPPREPAPTVEPPPAQFNFASHLFRLNDVRADKPAYIDDAGVTSYAQLEHRSRRFASALRSLGVHAEERILLVM
LDTAELPVAFLGALYAGVVPVVANTLLTATDYRYMLTHSHARAVIASGPLVPTVEQALSEAEQDGCLLIVSQPVHFDPPP
MHVLSRLIDGAEPLLKPNASSGDDIAFWLYSSGSTGKPKGTVHTHANLYWTAELYAKPILGINERDVVFSAAKLFFAYGL
GNALTFPLTVGATTVLMAERPTPDAVFRRLVEHRPTIFYGVPTLYASMLVSPNLPPREQVAMRICASAGEALPREIGERF
TAHFGCEILDGIGSTEMLHIFLSNRAGEVEYGTTGRPVPGYEVELRDEAGRTVPDGEVGDLFIKGPSAALMYWSNREKSR
ATFLGEWIRSGDKYCRLPNGCYQYAGRSDDMLKVSGQYVSPVEVEMALVQHTAVLEAAVVGVDHGGLVKTRAFVVLKQTA
DACDTLADDLKSFVKGKLAPHKYPRDIVFVDDLPKTATGKIQRFKLREQLTS

Sequences:

>Translated_532_residues
MQALPESPPREPAPTVEPPPAQFNFASHLFRLNDVRADKPAYIDDAGVTSYAQLEHRSRRFASALRSLGVHAEERILLVM
LDTAELPVAFLGALYAGVVPVVANTLLTATDYRYMLTHSHARAVIASGPLVPTVEQALSEAEQDGCLLIVSQPVHFDPPP
MHVLSRLIDGAEPLLKPNASSGDDIAFWLYSSGSTGKPKGTVHTHANLYWTAELYAKPILGINERDVVFSAAKLFFAYGL
GNALTFPLTVGATTVLMAERPTPDAVFRRLVEHRPTIFYGVPTLYASMLVSPNLPPREQVAMRICASAGEALPREIGERF
TAHFGCEILDGIGSTEMLHIFLSNRAGEVEYGTTGRPVPGYEVELRDEAGRTVPDGEVGDLFIKGPSAALMYWSNREKSR
ATFLGEWIRSGDKYCRLPNGCYQYAGRSDDMLKVSGQYVSPVEVEMALVQHTAVLEAAVVGVDHGGLVKTRAFVVLKQTA
DACDTLADDLKSFVKGKLAPHKYPRDIVFVDDLPKTATGKIQRFKLREQLTS
>Mature_532_residues
MQALPESPPREPAPTVEPPPAQFNFASHLFRLNDVRADKPAYIDDAGVTSYAQLEHRSRRFASALRSLGVHAEERILLVM
LDTAELPVAFLGALYAGVVPVVANTLLTATDYRYMLTHSHARAVIASGPLVPTVEQALSEAEQDGCLLIVSQPVHFDPPP
MHVLSRLIDGAEPLLKPNASSGDDIAFWLYSSGSTGKPKGTVHTHANLYWTAELYAKPILGINERDVVFSAAKLFFAYGL
GNALTFPLTVGATTVLMAERPTPDAVFRRLVEHRPTIFYGVPTLYASMLVSPNLPPREQVAMRICASAGEALPREIGERF
TAHFGCEILDGIGSTEMLHIFLSNRAGEVEYGTTGRPVPGYEVELRDEAGRTVPDGEVGDLFIKGPSAALMYWSNREKSR
ATFLGEWIRSGDKYCRLPNGCYQYAGRSDDMLKVSGQYVSPVEVEMALVQHTAVLEAAVVGVDHGGLVKTRAFVVLKQTA
DACDTLADDLKSFVKGKLAPHKYPRDIVFVDDLPKTATGKIQRFKLREQLTS

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=536, Percent_Identity=28.3582089552239, Blast_Score=197, Evalue=3e-50,
Organism=Homo sapiens, GI38505220, Length=534, Percent_Identity=28.8389513108614, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI58082049, Length=533, Percent_Identity=28.3302063789869, Blast_Score=185, Evalue=1e-46,
Organism=Homo sapiens, GI122937307, Length=535, Percent_Identity=27.8504672897196, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI157311624, Length=533, Percent_Identity=27.9549718574109, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI157311622, Length=533, Percent_Identity=27.9549718574109, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI115511026, Length=536, Percent_Identity=27.4253731343284, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI28416953, Length=522, Percent_Identity=28.3524904214559, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI187761345, Length=556, Percent_Identity=26.7985611510791, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI187761343, Length=556, Percent_Identity=26.7985611510791, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI8923896, Length=535, Percent_Identity=26.9158878504673, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI156151445, Length=513, Percent_Identity=25.7309941520468, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI253970489, Length=548, Percent_Identity=26.2773722627737, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI42544134, Length=378, Percent_Identity=24.8677248677249, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI13375727, Length=542, Percent_Identity=23.8007380073801, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI195947378, Length=406, Percent_Identity=22.1674876847291, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI38524616, Length=530, Percent_Identity=24.3396226415094, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI13236579, Length=477, Percent_Identity=22.4318658280922, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788107, Length=557, Percent_Identity=28.9048473967684, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1790505, Length=541, Percent_Identity=27.1719038817006, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI145693145, Length=502, Percent_Identity=28.2868525896414, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI221142682, Length=515, Percent_Identity=26.7961165048544, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1786810, Length=520, Percent_Identity=25.3846153846154, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789201, Length=364, Percent_Identity=27.4725274725275, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1786529, Length=529, Percent_Identity=26.4650283553875, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786801, Length=526, Percent_Identity=25.6653992395437, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788595, Length=338, Percent_Identity=25.1479289940828, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71994703, Length=516, Percent_Identity=26.1627906976744, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71994694, Length=516, Percent_Identity=26.1627906976744, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71994690, Length=516, Percent_Identity=26.1627906976744, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17558820, Length=510, Percent_Identity=24.5098039215686, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17531443, Length=499, Percent_Identity=25.6513026052104, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17557194, Length=527, Percent_Identity=26.3757115749526, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=521, Percent_Identity=24.7600767754319, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=525, Percent_Identity=26.0952380952381, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=518, Percent_Identity=24.1312741312741, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32563687, Length=493, Percent_Identity=22.920892494929, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=507, Percent_Identity=25.8382642998028, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=536, Percent_Identity=25.1865671641791, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=560, Percent_Identity=24.2857142857143, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982997, Length=560, Percent_Identity=24.2857142857143, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=380, Percent_Identity=23.4210526315789, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323182, Length=551, Percent_Identity=26.8602540834846, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319699, Length=509, Percent_Identity=26.7190569744597, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6319264, Length=376, Percent_Identity=29.5212765957447, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI18859661, Length=514, Percent_Identity=27.431906614786, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24581924, Length=513, Percent_Identity=26.5107212475634, Blast_Score=145, Evalue=9e-35,
Organism=Drosophila melanogaster, GI62472339, Length=525, Percent_Identity=26.6666666666667, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24667955, Length=524, Percent_Identity=26.5267175572519, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24667959, Length=379, Percent_Identity=28.4960422163588, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24656500, Length=497, Percent_Identity=23.7424547283702, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355181, Length=507, Percent_Identity=23.0769230769231, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355225, Length=537, Percent_Identity=23.8361266294227, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648260, Length=359, Percent_Identity=24.7910863509749, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648676, Length=383, Percent_Identity=27.9373368146214, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI20130357, Length=519, Percent_Identity=23.121387283237, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922652, Length=512, Percent_Identity=22.0703125, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24653035, Length=384, Percent_Identity=26.0416666666667, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356441, Length=498, Percent_Identity=21.285140562249, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI161076582, Length=399, Percent_Identity=25.062656641604, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648255, Length=487, Percent_Identity=21.9712525667351, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648253, Length=487, Percent_Identity=21.7659137577002, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21356947, Length=515, Percent_Identity=20.9708737864078, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 58147; Mature: 58147

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQALPESPPREPAPTVEPPPAQFNFASHLFRLNDVRADKPAYIDDAGVTSYAQLEHRSRR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHH
FASALRSLGVHAEERILLVMLDTAELPVAFLGALYAGVVPVVANTLLTATDYRYMLTHSH
HHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
ARAVIASGPLVPTVEQALSEAEQDGCLLIVSQPVHFDPPPMHVLSRLIDGAEPLLKPNAS
CCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCHHCCCCCC
SGDDIAFWLYSSGSTGKPKGTVHTHANLYWTAELYAKPILGINERDVVFSAAKLFFAYGL
CCCCEEEEEEECCCCCCCCCEEEECCEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
GNALTFPLTVGATTVLMAERPTPDAVFRRLVEHRPTIFYGVPTLYASMLVSPNLPPREQV
CCCEEEEEECCCEEEEEECCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCHHHH
AMRICASAGEALPREIGERFTAHFGCEILDGIGSTEMLHIFLSNRAGEVEYGTTGRPVPG
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCEEEEEEECCCCCCEEECCCCCCCCC
YEVELRDEAGRTVPDGEVGDLFIKGPSAALMYWSNREKSRATFLGEWIRSGDKYCRLPNG
CEEEECCCCCCCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHCCCCEEECCCH
CYQYAGRSDDMLKVSGQYVSPVEVEMALVQHTAVLEAAVVGVDHGGLVKTRAFVVLKQTA
HHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEEEHHHH
DACDTLADDLKSFVKGKLAPHKYPRDIVFVDDLPKTATGKIQRFKLREQLTS
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQALPESPPREPAPTVEPPPAQFNFASHLFRLNDVRADKPAYIDDAGVTSYAQLEHRSRR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHH
FASALRSLGVHAEERILLVMLDTAELPVAFLGALYAGVVPVVANTLLTATDYRYMLTHSH
HHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
ARAVIASGPLVPTVEQALSEAEQDGCLLIVSQPVHFDPPPMHVLSRLIDGAEPLLKPNAS
CCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCHHCCCCCC
SGDDIAFWLYSSGSTGKPKGTVHTHANLYWTAELYAKPILGINERDVVFSAAKLFFAYGL
CCCCEEEEEEECCCCCCCCCEEEECCEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
GNALTFPLTVGATTVLMAERPTPDAVFRRLVEHRPTIFYGVPTLYASMLVSPNLPPREQV
CCCEEEEEECCCEEEEEECCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCHHHH
AMRICASAGEALPREIGERFTAHFGCEILDGIGSTEMLHIFLSNRAGEVEYGTTGRPVPG
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCEEEEEEECCCCCCEEECCCCCCCCC
YEVELRDEAGRTVPDGEVGDLFIKGPSAALMYWSNREKSRATFLGEWIRSGDKYCRLPNG
CEEEECCCCCCCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHCCCCEEECCCH
CYQYAGRSDDMLKVSGQYVSPVEVEMALVQHTAVLEAAVVGVDHGGLVKTRAFVVLKQTA
HHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEEEHHHH
DACDTLADDLKSFVKGKLAPHKYPRDIVFVDDLPKTATGKIQRFKLREQLTS
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]