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The map label for this gene is rutR [H]

Identifier: 186476295

GI number: 186476295

Start: 1736756

End: 1737460

Strand: Direct

Name: rutR [H]

Synonym: Bphy_1537

Alternate gene names: 186476295

Gene position: 1736756-1737460 (Clockwise)

Preceding gene: 186476283

Following gene: 186476300

Centisome position: 49.92

GC content: 61.42

Gene sequence:

>705_bases
ATGAGACACGACAAGCCAGTGGAAGAAACGGCAGTGAGCGGCATGAACAATGACGACGCGCCCGCACCTTTGCGGCGGCG
CAAGGCTCACATCCGCGAGAGCAACGAAGCGCATCTGCTCGCATGCGCGGAGGCCGTTTTTGCGGAGCGGGGCTTCGAAG
GCGCGAGCACGGCGATGATCGCGGAACGCGCGGGCTTACCGAAAGCCAACGTCCATTACTACTTCCCAACGAAGCTCGCG
CTCTATCGCCGCGTGCTCGAAGATCTGTTCGAAGACTGGCATCGCGCGGCCAATACGTTTGAGGGCAGCGACGACCCCGT
CGAAGCGATCGGCGGCTATGTGCGCGCGAAAATGGAATTGTCGCGACGGCGGCCGCTCGGCTCGAAGGTGTGGGCGAACG
AGATCATTCACGGCGCGGTACACATGGCCGATATTCTCGAACAACGCGTGAAGCCGTGGCTCGATACGCGCGTGACCGTG
ATCGACGACTGGATCGCGCGCGGACTGCTCGCGAACGTCGATGCGCAAACGCTGCTGTACATGATCTGGGCAACGACCCA
GCACTATGCGGATTTCGATGCGCAGATACGCGCACTCGGCGGCAAACGTGCATTGTCACAAAAGGCTTTCGACGCAACGA
CGGATCAGGTCGTGCAACTCGTGATTCGCGCGTGCGGGGCGAAATCGCCGGCTGCGAAGACGTAA

Upstream 100 bases:

>100_bases
CGGTGTGCGCAGAAAGACCGCGAACGCGGATTCCGGCCGATTGCAAGGCGAGCGGCTAGAATGAAGCGCGACGCGGCACA
CAGGCCGCGCGAGGCCAGCG

Downstream 100 bases:

>100_bases
CGCCCGCTCGCGTTCAGTACGTTTCCAGATGCAGACGTCCTTCGCGCTTGAGCCTCTGCCATAGCGCGTTCCATTCGAGG
CCATGCTGATCGGCAATGTC

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MRHDKPVEETAVSGMNNDDAPAPLRRRKAHIRESNEAHLLACAEAVFAERGFEGASTAMIAERAGLPKANVHYYFPTKLA
LYRRVLEDLFEDWHRAANTFEGSDDPVEAIGGYVRAKMELSRRRPLGSKVWANEIIHGAVHMADILEQRVKPWLDTRVTV
IDDWIARGLLANVDAQTLLYMIWATTQHYADFDAQIRALGGKRALSQKAFDATTDQVVQLVIRACGAKSPAAKT

Sequences:

>Translated_234_residues
MRHDKPVEETAVSGMNNDDAPAPLRRRKAHIRESNEAHLLACAEAVFAERGFEGASTAMIAERAGLPKANVHYYFPTKLA
LYRRVLEDLFEDWHRAANTFEGSDDPVEAIGGYVRAKMELSRRRPLGSKVWANEIIHGAVHMADILEQRVKPWLDTRVTV
IDDWIARGLLANVDAQTLLYMIWATTQHYADFDAQIRALGGKRALSQKAFDATTDQVVQLVIRACGAKSPAAKT
>Mature_234_residues
MRHDKPVEETAVSGMNNDDAPAPLRRRKAHIRESNEAHLLACAEAVFAERGFEGASTAMIAERAGLPKANVHYYFPTKLA
LYRRVLEDLFEDWHRAANTFEGSDDPVEAIGGYVRAKMELSRRRPLGSKVWANEIIHGAVHMADILEQRVKPWLDTRVTV
IDDWIARGLLANVDAQTLLYMIWATTQHYADFDAQIRALGGKRALSQKAFDATTDQVVQLVIRACGAKSPAAKT

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=184, Percent_Identity=37.5, Blast_Score=134, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 26122; Mature: 26122

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHDKPVEETAVSGMNNDDAPAPLRRRKAHIRESNEAHLLACAEAVFAERGFEGASTAMI
CCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHH
AERAGLPKANVHYYFPTKLALYRRVLEDLFEDWHRAANTFEGSDDPVEAIGGYVRAKMEL
HHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SRRRPLGSKVWANEIIHGAVHMADILEQRVKPWLDTRVTVIDDWIARGLLANVDAQTLLY
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
MIWATTQHYADFDAQIRALGGKRALSQKAFDATTDQVVQLVIRACGAKSPAAKT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRHDKPVEETAVSGMNNDDAPAPLRRRKAHIRESNEAHLLACAEAVFAERGFEGASTAMI
CCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHH
AERAGLPKANVHYYFPTKLALYRRVLEDLFEDWHRAANTFEGSDDPVEAIGGYVRAKMEL
HHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SRRRPLGSKVWANEIIHGAVHMADILEQRVKPWLDTRVTVIDDWIARGLLANVDAQTLLY
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
MIWATTQHYADFDAQIRALGGKRALSQKAFDATTDQVVQLVIRACGAKSPAAKT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]