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The map label for this gene is thiC [H]

Identifier: 186476276

GI number: 186476276

Start: 1712223

End: 1714154

Strand: Direct

Name: thiC [H]

Synonym: Bphy_1518

Alternate gene names: 186476276

Gene position: 1712223-1714154 (Clockwise)

Preceding gene: 186476275

Following gene: 186476277

Centisome position: 49.21

GC content: 63.56

Gene sequence:

>1932_bases
ATGAACGCCAATCCGAAATTCATTTCCGCGAACGCGCACGTCGACGAGGCCGCCGTCGCGCCGCTGCCGAACTCGCGCAA
GGTCTACGTGACCGGTTCGCAACCCGACATCCGCGTGCCGATGCGTGAAATCACGCAAGCCGACACGCCGACGGGGTTCG
GCGGCGAAAAGAATCCGCCCATCTACGTGTACGACACGTCGGGCCCGTACACCGATCCCGACGCCAAAATCGACATCCGC
GCCGGCCTGCCCGCGCTGCGCCAGCGCTGGATCGAGGCGCGCGGCGACACGGAGTCCCTGCAGGGACTATCGAGCGAATA
CGGCCGCGAGCGCGCCGCCGATCCCGCCACCGCGGAACTGCGCTTTCCCGGCCTGCACCGCACGCCGCGCCGCGCGCAGC
CCGGCAAGAACGTGTCGCAGATGCACTACGCGAAGCAAGGCATCATCACGCCGGAAATGGAATACATCGCGATCCGCGAA
AACCAGCGCCGCGCCGAGTATCTGGAAAGCCTGAAGGCCAGCGGTCCGAACGGCGCGAAGCTCGCCGCGATGATGGGCCG
CCAGCATCCGGGCCAGGCGTTCGGCGCGACAGCATTCGGTCCGAACGGGCTGCAGGAGATCACGCCCGAGTTCGTGCGCG
AAGAAGTCGCGCGCGGCCGCGCAATCATCCCCGCGAACATCAATCACCCGGAAAGCGAGCCGATGATCATCGGCCGCAAC
TTCCTCGTGAAGATCAACGCGAACATCGGCAATTCGGCTGTGACGTCGTCGATCGGCGAGGAAGTCGACAAAATGACGTG
GGCAATCCGCTGGGGCGGCGACACCGTGATGGATCTGTCCACCGGCAAGCACATTCATGAGACGCGCGAATGGATCATCC
GCAATTCGCCTGTTCCCATCGGCACGGTGCCGATCTATCAGGCGCTCGAAAAGGTCAATGGCAAAGCCGAAGACCTGACG
TGGGAGATCTTCCGCGACACGCTGATCGAACAGGCCGAGCAAGGCGTCGACTACTTCACGATTCACGCCGGCGTGCGCCT
GCAATATGTGCCGCTCACCGCGAACCGGATGACGGGCATCGTGTCGCGCGGCGGCTCGATCATGGCGAAGTGGTGCCTTG
CGCATCACAAGGAAAGCTTCCTGTACGAGCACTTCGAAGACATCTGCGAAATCATGAAGGCGTACGACGTGAGCTTCTCG
CTCGGCGACGGCCTGCGCCCCGGCTCGATCTACGACGCGAACGACGAAGCGCAGCTCGGCGAACTGAAGACGCTCGGCGA
ACTCACGCAGATCGCGTGGAAGCACGACGTGCAGGTGATGATCGAAGGCCCCGGCCACGTGCCGATGCAGCTGATCAAGG
AGAACATGGACCTGCAGCTGGAATGGTGCGACGAAGCGCCGTTCTACACGCTCGGACCGCTGACGACGGACATCGCGCCA
GGCTATGACCACATCACGTCGGGCATCGGCGCGGCGATGATCGGCTGGTTCGGCACGGCGATGCTCTGCTACGTGACGCC
GAAGGAACACCTCGGTCTGCCGAACAAGGACGACGTGAAGACGGGCATCATCACGTACAAGCTTGCCGCGCACGCCGCCG
ATCTGGCGAAGGGCCATCCGGGCGCGCAGGTGCGCGACAACGCGCTGTCGAAAGCGCGCTTCGAGTTCCGCTGGGAAGAC
CAGTTCAACCTGGGCCTCGATCCCGACAAGGCGCGCGAATTCCACGACGAAACGCTGCCGAAGGACTCAGCAAAGGTCGC
GCATTTCTGCTCGATGTGCGGCCCGCACTTCTGCTCGATGAAGATCACGCAGGACGTGCGCGACTACGCTGCGAAGGAAG
GCATGAGCGACGCCGACGCGCTGAAGAAAGGGATGGAAGTGAAGGCCGTCGAGTTCATCAAGAGCGGCGCGGAGATCTAT
CAGCGCCAGTGA

Upstream 100 bases:

>100_bases
CCTGCCGCACCGTTTAAGTCTCCTCGCGAACCCGGTCCACTTCCCCTCCGTCTCGTTCTCCTGCGTAGCTGTAACGCCCC
ACAATTGCATGGAGACACGC

Downstream 100 bases:

>100_bases
CGCGCCCGTCGTTGCAACGTGCCCGGAAAGCGAAGCCCGCCAGTTGTTCATGGCGGGCTTTTTTGCGCGTTTTTACCGCA
TTGCACTGCAATGGCACAGG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MNANPKFISANAHVDEAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPPIYVYDTSGPYTDPDAKIDIR
AGLPALRQRWIEARGDTESLQGLSSEYGRERAADPATAELRFPGLHRTPRRAQPGKNVSQMHYAKQGIITPEMEYIAIRE
NQRRAEYLESLKASGPNGAKLAAMMGRQHPGQAFGATAFGPNGLQEITPEFVREEVARGRAIIPANINHPESEPMIIGRN
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLT
WEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYEHFEDICEIMKAYDVSFS
LGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLEWCDEAPFYTLGPLTTDIAP
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKTGIITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWED
QFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVRDYAAKEGMSDADALKKGMEVKAVEFIKSGAEIY
QRQ

Sequences:

>Translated_643_residues
MNANPKFISANAHVDEAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPPIYVYDTSGPYTDPDAKIDIR
AGLPALRQRWIEARGDTESLQGLSSEYGRERAADPATAELRFPGLHRTPRRAQPGKNVSQMHYAKQGIITPEMEYIAIRE
NQRRAEYLESLKASGPNGAKLAAMMGRQHPGQAFGATAFGPNGLQEITPEFVREEVARGRAIIPANINHPESEPMIIGRN
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLT
WEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYEHFEDICEIMKAYDVSFS
LGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLEWCDEAPFYTLGPLTTDIAP
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKTGIITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWED
QFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVRDYAAKEGMSDADALKKGMEVKAVEFIKSGAEIY
QRQ
>Mature_643_residues
MNANPKFISANAHVDEAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPPIYVYDTSGPYTDPDAKIDIR
AGLPALRQRWIEARGDTESLQGLSSEYGRERAADPATAELRFPGLHRTPRRAQPGKNVSQMHYAKQGIITPEMEYIAIRE
NQRRAEYLESLKASGPNGAKLAAMMGRQHPGQAFGATAFGPNGLQEITPEFVREEVARGRAIIPANINHPESEPMIIGRN
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLT
WEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYEHFEDICEIMKAYDVSFS
LGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLEWCDEAPFYTLGPLTTDIAP
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKTGIITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWED
QFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVRDYAAKEGMSDADALKKGMEVKAVEFIKSGAEIY
QRQ

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=641, Percent_Identity=68.798751950078, Blast_Score=904, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 71205; Mature: 71205

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNANPKFISANAHVDEAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPP
CCCCCCEEECCCCCCCHHCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCC
IYVYDTSGPYTDPDAKIDIRAGLPALRQRWIEARGDTESLQGLSSEYGRERAADPATAEL
EEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEE
RFPGLHRTPRRAQPGKNVSQMHYAKQGIITPEMEYIAIRENQRRAEYLESLKASGPNGAK
ECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHH
LAAMMGRQHPGQAFGATAFGPNGLQEITPEFVREEVARGRAIIPANINHPESEPMIIGRN
HHHHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCE
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPI
EEEEEECCCCCHHHHHHHHHHHHHEEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCC
GTVPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGI
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECHHHHHHH
VSRGGSIMAKWCLAHHKESFLYEHFEDICEIMKAYDVSFSLGDGLRPGSIYDANDEAQLG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCCCHHH
ELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLEWCDEAPFYTLGPLTTDIAP
HHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCC
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKTGIITYKLAAHAADLAKGHP
CHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCCCC
GAQVRDNALSKARFEFRWEDQFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSM
CCCHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
KITQDVRDYAAKEGMSDADALKKGMEVKAVEFIKSGAEIYQRQ
HHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNANPKFISANAHVDEAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPP
CCCCCCEEECCCCCCCHHCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCC
IYVYDTSGPYTDPDAKIDIRAGLPALRQRWIEARGDTESLQGLSSEYGRERAADPATAEL
EEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEE
RFPGLHRTPRRAQPGKNVSQMHYAKQGIITPEMEYIAIRENQRRAEYLESLKASGPNGAK
ECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHH
LAAMMGRQHPGQAFGATAFGPNGLQEITPEFVREEVARGRAIIPANINHPESEPMIIGRN
HHHHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCE
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPI
EEEEEECCCCCHHHHHHHHHHHHHEEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCC
GTVPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGI
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECHHHHHHH
VSRGGSIMAKWCLAHHKESFLYEHFEDICEIMKAYDVSFSLGDGLRPGSIYDANDEAQLG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCCCHHH
ELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLEWCDEAPFYTLGPLTTDIAP
HHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCC
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKTGIITYKLAAHAADLAKGHP
CHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCCCC
GAQVRDNALSKARFEFRWEDQFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSM
CCCHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
KITQDVRDYAAKEGMSDADALKKGMEVKAVEFIKSGAEIYQRQ
HHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA