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The map label for this gene is nreB [H]

Identifier: 186476270

GI number: 186476270

Start: 1705096

End: 1706640

Strand: Direct

Name: nreB [H]

Synonym: Bphy_1512

Alternate gene names: 186476270

Gene position: 1705096-1706640 (Clockwise)

Preceding gene: 186476264

Following gene: 186476271

Centisome position: 49.01

GC content: 66.28

Gene sequence:

>1545_bases
GTGTCCGGTGACACACGAGCGGGGGCCGACGCAAGACTCGCGACCAGGCTGGCCAACTGGATGCACCAGCGCAGCTGGGC
AATCGCGATGACGGTCGCGATCGTCATCACGTGCGGCGGCCTCGTCATTCTCGAAACGGCGCGGCAGCGTATCGCATCGG
AATACGAAGCGGCGCTCGAAGCGCGCGGCGTGAGCATGCAGCTGGGCGAACTGGACGGGCATCTCGCGCGGTTGCAGGCG
CACGAAAGCCGCTTTCTGCTGACGAAGGACGACGCCGATGCCCACGGCTTTTGCGCGCGTGCCGCGCAAATCCATCGCGG
CATCCGCTCGCTCGACGCGTATTTCCGACGCGCCGCCGACAATGCCGAACTGGCCAGCTTCACGCAAATCGCCGCGCTGA
TCGAGGCGCGCATCGCCAGCGGCGCACAGGCGTTGCAACAGGCTTCGCCCCATCCGCTCGATCTCGGCGCGTGCGGCAGC
GCAATGCAGAAAAGCGGCGTCGATGCTTCGCTCGTGACGAGCACGCTCGCTTTGCTGCGCGACAACGAAGAGCGCCGCGC
GCAACGCGCGCTTGATGCGAGCCGCGCCGACCAGCGCGTATCGACGCTGGTCGCGGCGGGCCTGTCCGCGCTCAATATCG
TGTTGTTCATTCTGCTGTTCCGCAACCTGGGCATCCAGCTGGACAGTCAGGCGCGCGTGCAAAAACAGTTGATCACGCAG
CAGGAGGAACTCGATCGGCTGGTCAACGAGCGCACGCGGCAACTCGAAGCGTTGGGCTGGCACCTGCAGGCCGTCAGCGA
AAACGAAAAGACCCAGCTCGCGCGCGAACTGCACGACGAACTCGGCGCGATTCTCACAGCGAGCAAGATGGACGTCGCGT
GGGCGCGCCGCAAACTGCAGGAGGCCGAGCCCGTCGTCGCCGAGAAACTGACGCGCGCGCTGGCGACGCTCGATCAGGGC
ATCGCGCTCAAGCGCCGCATCATCGAAGACATGCGGCCGACCGTGCTCGCGAACTTCGGCCTCGTCACCGCGCTGCGCAC
GCTCGGCGACGAAGCCGCGCAACGCAACGGCTGGGCGCTCGACCTGCATCTGCCCGACGACGAACTGCATCTGGATGAGC
AGGTCGAGATCGCGCTGTTTCGCGTCGCGCAGGAATCGCTGACCAATGCAGCGAAATACGCACGTGCAACGCAAATCATG
ATCGCGTTGAGCGCGGGCCACGGCGAAGTGACGCTGCACATCGCCGATAACGGCATCGGCATCATGCCCGGCGACCTGAC
GCGCACGCATACGCACGGGCTGATGGGCATGCGCCAGCGCGTGGCCGCGCGCGGTGGCCGATTCGATGTCCGGCGCGGGG
AGTTGCACGGCACAGAGATTCGCGTGGTGCTGCCCGTGCCGACCGTCGGCCTGATAGAAGAACCAGGAAACGAACCAGAC
CCGAGCGAGCCACGCGGCGAATCAACCGATGCCGCGCCAGACGGCATCGTCGCGCCTGAGCGCGACAGGTCCGTCGGACG
GACACCCGTCGGACGCGTTTCATGA

Upstream 100 bases:

>100_bases
GCCGACACCCGCTTTCGGCCAGCCATCCGCCGCGCCCGCGCGAACGCAAGCGCGCCGCATGACGCTACACTGTCGATGAC
TTCATCGCATGGAGACGGCG

Downstream 100 bases:

>100_bases
GACCCATGAAGCCTTCGACGCGCCGCGTAGGACTGCACCGACAGGAAAATCGTCGCGTCTCCGACAGCATCGCGGCAACG
CGTCTTTTACCATGAAATCA

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSGDTRAGADARLATRLANWMHQRSWAIAMTVAIVITCGGLVILETARQRIASEYEAALEARGVSMQLGELDGHLARLQA
HESRFLLTKDDADAHGFCARAAQIHRGIRSLDAYFRRAADNAELASFTQIAALIEARIASGAQALQQASPHPLDLGACGS
AMQKSGVDASLVTSTLALLRDNEERRAQRALDASRADQRVSTLVAAGLSALNIVLFILLFRNLGIQLDSQARVQKQLITQ
QEELDRLVNERTRQLEALGWHLQAVSENEKTQLARELHDELGAILTASKMDVAWARRKLQEAEPVVAEKLTRALATLDQG
IALKRRIIEDMRPTVLANFGLVTALRTLGDEAAQRNGWALDLHLPDDELHLDEQVEIALFRVAQESLTNAAKYARATQIM
IALSAGHGEVTLHIADNGIGIMPGDLTRTHTHGLMGMRQRVAARGGRFDVRRGELHGTEIRVVLPVPTVGLIEEPGNEPD
PSEPRGESTDAAPDGIVAPERDRSVGRTPVGRVS

Sequences:

>Translated_514_residues
MSGDTRAGADARLATRLANWMHQRSWAIAMTVAIVITCGGLVILETARQRIASEYEAALEARGVSMQLGELDGHLARLQA
HESRFLLTKDDADAHGFCARAAQIHRGIRSLDAYFRRAADNAELASFTQIAALIEARIASGAQALQQASPHPLDLGACGS
AMQKSGVDASLVTSTLALLRDNEERRAQRALDASRADQRVSTLVAAGLSALNIVLFILLFRNLGIQLDSQARVQKQLITQ
QEELDRLVNERTRQLEALGWHLQAVSENEKTQLARELHDELGAILTASKMDVAWARRKLQEAEPVVAEKLTRALATLDQG
IALKRRIIEDMRPTVLANFGLVTALRTLGDEAAQRNGWALDLHLPDDELHLDEQVEIALFRVAQESLTNAAKYARATQIM
IALSAGHGEVTLHIADNGIGIMPGDLTRTHTHGLMGMRQRVAARGGRFDVRRGELHGTEIRVVLPVPTVGLIEEPGNEPD
PSEPRGESTDAAPDGIVAPERDRSVGRTPVGRVS
>Mature_513_residues
SGDTRAGADARLATRLANWMHQRSWAIAMTVAIVITCGGLVILETARQRIASEYEAALEARGVSMQLGELDGHLARLQAH
ESRFLLTKDDADAHGFCARAAQIHRGIRSLDAYFRRAADNAELASFTQIAALIEARIASGAQALQQASPHPLDLGACGSA
MQKSGVDASLVTSTLALLRDNEERRAQRALDASRADQRVSTLVAAGLSALNIVLFILLFRNLGIQLDSQARVQKQLITQQ
EELDRLVNERTRQLEALGWHLQAVSENEKTQLARELHDELGAILTASKMDVAWARRKLQEAEPVVAEKLTRALATLDQGI
ALKRRIIEDMRPTVLANFGLVTALRTLGDEAAQRNGWALDLHLPDDELHLDEQVEIALFRVAQESLTNAAKYARATQIMI
ALSAGHGEVTLHIADNGIGIMPGDLTRTHTHGLMGMRQRVAARGGRFDVRRGELHGTEIRVVLPVPTVGLIEEPGNEPDP
SEPRGESTDAAPDGIVAPERDRSVGRTPVGRVS

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=244, Percent_Identity=29.5081967213115, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788812, Length=197, Percent_Identity=28.9340101522843, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI87082311, Length=216, Percent_Identity=28.7037037037037, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55932; Mature: 55801

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDTRAGADARLATRLANWMHQRSWAIAMTVAIVITCGGLVILETARQRIASEYEAALE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ARGVSMQLGELDGHLARLQAHESRFLLTKDDADAHGFCARAAQIHRGIRSLDAYFRRAAD
HCCCEEEHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
NAELASFTQIAALIEARIASGAQALQQASPHPLDLGACGSAMQKSGVDASLVTSTLALLR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHH
DNEERRAQRALDASRADQRVSTLVAAGLSALNIVLFILLFRNLGIQLDSQARVQKQLITQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHH
QEELDRLVNERTRQLEALGWHLQAVSENEKTQLARELHDELGAILTASKMDVAWARRKLQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAEPVVAEKLTRALATLDQGIALKRRIIEDMRPTVLANFGLVTALRTLGDEAAQRNGWAL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
DLHLPDDELHLDEQVEIALFRVAQESLTNAAKYARATQIMIALSAGHGEVTLHIADNGIG
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCCE
IMPGDLTRTHTHGLMGMRQRVAARGGRFDVRRGELHGTEIRVVLPVPTVGLIEEPGNEPD
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
PSEPRGESTDAAPDGIVAPERDRSVGRTPVGRVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SGDTRAGADARLATRLANWMHQRSWAIAMTVAIVITCGGLVILETARQRIASEYEAALE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ARGVSMQLGELDGHLARLQAHESRFLLTKDDADAHGFCARAAQIHRGIRSLDAYFRRAAD
HCCCEEEHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
NAELASFTQIAALIEARIASGAQALQQASPHPLDLGACGSAMQKSGVDASLVTSTLALLR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHH
DNEERRAQRALDASRADQRVSTLVAAGLSALNIVLFILLFRNLGIQLDSQARVQKQLITQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHH
QEELDRLVNERTRQLEALGWHLQAVSENEKTQLARELHDELGAILTASKMDVAWARRKLQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAEPVVAEKLTRALATLDQGIALKRRIIEDMRPTVLANFGLVTALRTLGDEAAQRNGWAL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
DLHLPDDELHLDEQVEIALFRVAQESLTNAAKYARATQIMIALSAGHGEVTLHIADNGIG
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCCE
IMPGDLTRTHTHGLMGMRQRVAARGGRFDVRRGELHGTEIRVVLPVPTVGLIEEPGNEPD
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
PSEPRGESTDAAPDGIVAPERDRSVGRTPVGRVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]