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The map label for this gene is ntrB [H]

Identifier: 186476238

GI number: 186476238

Start: 1670590

End: 1671732

Strand: Reverse

Name: ntrB [H]

Synonym: Bphy_1480

Alternate gene names: 186476238

Gene position: 1671732-1670590 (Counterclockwise)

Preceding gene: 186476239

Following gene: 186476237

Centisome position: 48.05

GC content: 61.94

Gene sequence:

>1143_bases
ATGGTATTGAAGAACCTGATCAAGGCCAGAAAAGGGCATGCCGACGCACTGTCGGATGACGCGCAACTCGTCGACTCCGG
TTTGCTGCCGGGCTTCGAGGCGTTGCCCACTGTCGTGCTCGTGCTCGACAAGCGGACGCTGCGGGTCGCGTTCGCGAATC
CGTCGGCCGAGTCGATGCTCGAAATGTCGCGCCGGCAACTCACGCAAATGGCGTGGCCCGACATCTTCGCGAACGGCGAG
GAGCTGACGGCGACTATCGCTGCCATCGCCGCTCACCGTTTTCATGCGACGCATCTCGATGCCGTGCTCGAGCGCCCAGG
CCACGAGCCGTTGCATGTGCATGCTGTCGTCGGCTTTCTGGAAAGCGCGCAGGACTACGTGCTGCTCGAACTGTTCGAGA
ACGAGCGGCATCTGAGATCGGATCGCGAAGAGCGCATTCACGATCTCACGGCAGTCAACAAGCAGTTGATCCGCAATCTC
GCGCATGAGATCAAGAATCCGCTCGGCGGCATTCGCGGCGCGGCGCAGTTGCTCGAGTTCGAACTCGGCGAGCGCGAACG
CGACGAACTGCGCGAGTACACACAGGTCGTCATCAAGGAATCGGACCGGCTGCAGACGCTCGTCGACCGGCTGCTCGAGC
CGCATCGTCATCCGCATATCGTCGGCGACGTGAACATCCACGAAGTGTGCGAACGCGTGCGCGCCGTGATTCTCGCGGAG
TTTCCGCGTGGCCTCACGATCGAGCGCGACTACGACGTGAGCGTGCCTGATCTGCGCGGCGACAAGGAGCAGCTGATCCA
GGCGCTGCTGAACATCGTGCGCAATGCGGCCGAGGCATTGCGCGAACGCATTTCGCAGGGTGATGCCCGCATCGAGTTGC
GCACGCGCGTCGCGCGCAAGGTGACGATCGCGAAACGTCTTTGCAAGCTGGCACTGGACTTGCATATCGTCGACAACGGA
CCCGGCATTCCCGAGGACATACGCGAACGCATCTTCTATCCGCTCGTATCGGGCCGTGAAGATGGCAGCGGTCTTGGCCT
GACGCTCGCGCAGACCTTCGTTCAACAGCACGAGGGGATGATCGAAGTGGAGAGCCGTCCGGGTCATACCGAGTTTCAGA
TCCTGCTGCCGCTCGACGTCTGA

Upstream 100 bases:

>100_bases
CTGCGATCGGTCATGAAGGGACGGCGGCGCCGTCCCTTTTTCGTTGGATCGAAAGCCGTGACGTTTTTGTGGCAAACCGC
TATCGAGACCCGACTGCAAG

Downstream 100 bases:

>100_bases
CAAACGAGTGCACACACACGCAATACAAGACTTCTGACGGACCTATATGAAGCCGATCTGGATAGTAGACGACGATCAAT
CGATCCGTTGGGTGCTCGAA

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MVLKNLIKARKGHADALSDDAQLVDSGLLPGFEALPTVVLVLDKRTLRVAFANPSAESMLEMSRRQLTQMAWPDIFANGE
ELTATIAAIAAHRFHATHLDAVLERPGHEPLHVHAVVGFLESAQDYVLLELFENERHLRSDREERIHDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERDELREYTQVVIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVILAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAEALRERISQGDARIELRTRVARKVTIAKRLCKLALDLHIVDNG
PGIPEDIRERIFYPLVSGREDGSGLGLTLAQTFVQQHEGMIEVESRPGHTEFQILLPLDV

Sequences:

>Translated_380_residues
MVLKNLIKARKGHADALSDDAQLVDSGLLPGFEALPTVVLVLDKRTLRVAFANPSAESMLEMSRRQLTQMAWPDIFANGE
ELTATIAAIAAHRFHATHLDAVLERPGHEPLHVHAVVGFLESAQDYVLLELFENERHLRSDREERIHDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERDELREYTQVVIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVILAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAEALRERISQGDARIELRTRVARKVTIAKRLCKLALDLHIVDNG
PGIPEDIRERIFYPLVSGREDGSGLGLTLAQTFVQQHEGMIEVESRPGHTEFQILLPLDV
>Mature_380_residues
MVLKNLIKARKGHADALSDDAQLVDSGLLPGFEALPTVVLVLDKRTLRVAFANPSAESMLEMSRRQLTQMAWPDIFANGE
ELTATIAAIAAHRFHATHLDAVLERPGHEPLHVHAVVGFLESAQDYVLLELFENERHLRSDREERIHDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERDELREYTQVVIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVILAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAEALRERISQGDARIELRTRVARKVTIAKRLCKLALDLHIVDNG
PGIPEDIRERIFYPLVSGREDGSGLGLTLAQTFVQQHEGMIEVESRPGHTEFQILLPLDV

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=351, Percent_Identity=39.3162393162393, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1788549, Length=357, Percent_Identity=26.6106442577031, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790436, Length=261, Percent_Identity=27.2030651340996, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1790346, Length=243, Percent_Identity=26.3374485596708, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42868; Mature: 42868

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLKNLIKARKGHADALSDDAQLVDSGLLPGFEALPTVVLVLDKRTLRVAFANPSAESML
CCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHEECCCEEEEEECCCCHHHHH
EMSRRQLTQMAWPDIFANGEELTATIAAIAAHRFHATHLDAVLERPGHEPLHVHAVVGFL
HHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHH
ESAQDYVLLELFENERHLRSDREERIHDLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEF
HCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGERERDELREYTQVVIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVILAE
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHH
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAEALRERISQGDARIELRTRVARK
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
VTIAKRLCKLALDLHIVDNGPGIPEDIRERIFYPLVSGREDGSGLGLTLAQTFVQQHEGM
HHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCE
IEVESRPGHTEFQILLPLDV
EEECCCCCCCEEEEEEECCC
>Mature Secondary Structure
MVLKNLIKARKGHADALSDDAQLVDSGLLPGFEALPTVVLVLDKRTLRVAFANPSAESML
CCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHEECCCEEEEEECCCCHHHHH
EMSRRQLTQMAWPDIFANGEELTATIAAIAAHRFHATHLDAVLERPGHEPLHVHAVVGFL
HHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHH
ESAQDYVLLELFENERHLRSDREERIHDLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEF
HCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGERERDELREYTQVVIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVILAE
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHH
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAEALRERISQGDARIELRTRVARK
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
VTIAKRLCKLALDLHIVDNGPGIPEDIRERIFYPLVSGREDGSGLGLTLAQTFVQQHEGM
HHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCE
IEVESRPGHTEFQILLPLDV
EEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]