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The map label for this gene is glnG [H]

Identifier: 186476237

GI number: 186476237

Start: 1669029

End: 1670543

Strand: Reverse

Name: glnG [H]

Synonym: Bphy_1479

Alternate gene names: 186476237

Gene position: 1670543-1669029 (Counterclockwise)

Preceding gene: 186476238

Following gene: 186476235

Centisome position: 48.02

GC content: 63.96

Gene sequence:

>1515_bases
ATGAAGCCGATCTGGATAGTAGACGACGATCAATCGATCCGTTGGGTGCTCGAAAAAGCACTCGCCCGCGAGAACTTTGC
GACGCGCAGCTTCGCGAACGTGCGCGAAGCCGCGGGCGCGCTGGAACACGACAGCCCGCAGGTGCTGGTCTCCGACATCA
GGATGCCGGGCGGCTCCGGGCTTGAACTGCTGCAGACCGTGCGCGACCGCGTGCCGGGTCTGCCCGTCATCATCATGACG
GCGTTCTCGGATCTCGATAGCGCTGTGGCCGCGTTTCAGGGCGGCGCGTTCGAGTACCTCGCGAAGCCGTTCGACGTCGA
CAAGGCCGTGGAGCTGATTCGCCGTGCCGTCGACGAGAGCATGCGCGGCGAGCAGACGTGGGACGATCGCGTCGCCGAAG
CGCCCGAGATGCTCGGCCAGGCGCCCGCGATGCAGGACATGTTTCGCGCGATCGGCCGTCTGTCGCATTCGGCCGCGACC
GTGCTCATTACAGGCGAGTCAGGCACCGGTAAGGAGCTTGTCGCGCGCGCCTTGCACCGACATAGCCCACGCGCGAACGG
TCCGTTCATCGCATTGAACACGGCAGCGATTCCGAAAGATCTGCTGGAATCCGAACTGTTCGGCCACGAACGCGGCGCGT
TCACGGGCGCACAGGCGATGCGACAGGGCCGTTTCGAACAGGCTGAGAACGGCACGTTGTTTCTGGATGAAATCGGCGAC
ATGCCGTTCGATTTGCAGACGCGTCTGTTGCGCGTGCTGTCGGATGGCCAGTTCTATCGCGTCGGCGGACACAACCCGTT
GCGCGCGAATGTGCGGGTGATTGCCGCGACACATCAGAATCTCGAATCGCGCGTGCGTCAGGGACTGTTCCGCGAGGACT
TGTATCACCGTCTCAATGTGATTCGTCTGCGTCTGCCTGCGTTGCGCGAGCGCAGCGAAGACATTCCGCTGCTCACGCGC
CATTTCCTGCAGAAGAGCGCGCGCGACCTCGGCGTCGAGCCGAAGCGGGTATCAGACGAGGCGCTCGTTTATATGACGTC
GCTGCCTTTTCCGGGCAACGTGCGGCAACTCGAAAATCTGGCGAACTGGCTGACCGTCATGGCGCCCGCGCAGACGATCG
AGATCAAGGACTTGCCGCCCGATCTCGTGTCGACGCAGCAGAGCGGGGCGGAGCACGCGACGTCTGCCGTGTCCATGTCG
AGCGAAGCCGCGTCGGGCAACGGCGGCGTGACGAACGGTGTTGCGCCCGCTGTGGGCATGCCCGTCGCGACGGGCGGCGC
AGCGATTTCGCCGCCTGTGGCTGCATGGGAAAACGGCTTGCGCACGGAAGTCGCGAAACTCTTGCGCGAGAATGCCGCCG
ACGTGATGGACGAACTGTCGCGCCGATTCGAGGCGGCGGTGATACGCGAAGCGCTCGATTTCACGCGCGGTCGCAAGGTC
GAGGCAGCGGAGCGGCTCGGCATCGGGCGCAATACGATTACGCGCAAGATTCAGGAGTTGAATCTTGAGCCGTAG

Upstream 100 bases:

>100_bases
GAGAGCCGTCCGGGTCATACCGAGTTTCAGATCCTGCTGCCGCTCGACGTCTGACAAACGAGTGCACACACACGCAATAC
AAGACTTCTGACGGACCTAT

Downstream 100 bases:

>100_bases
GGGCGGATGCGAGCGGGCGTGACGCACTTACGCTCACGTGTGGCACAACGAAAGCGGCTCGACGGCGTGATGCGCCATCG
AGCCGCTTTGTCTTTGCTGC

Product: nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MKPIWIVDDDQSIRWVLEKALARENFATRSFANVREAAGALEHDSPQVLVSDIRMPGGSGLELLQTVRDRVPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVDESMRGEQTWDDRVAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQAMRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPALRERSEDIPLLTR
HFLQKSARDLGVEPKRVSDEALVYMTSLPFPGNVRQLENLANWLTVMAPAQTIEIKDLPPDLVSTQQSGAEHATSAVSMS
SEAASGNGGVTNGVAPAVGMPVATGGAAISPPVAAWENGLRTEVAKLLRENAADVMDELSRRFEAAVIREALDFTRGRKV
EAAERLGIGRNTITRKIQELNLEP

Sequences:

>Translated_504_residues
MKPIWIVDDDQSIRWVLEKALARENFATRSFANVREAAGALEHDSPQVLVSDIRMPGGSGLELLQTVRDRVPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVDESMRGEQTWDDRVAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQAMRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPALRERSEDIPLLTR
HFLQKSARDLGVEPKRVSDEALVYMTSLPFPGNVRQLENLANWLTVMAPAQTIEIKDLPPDLVSTQQSGAEHATSAVSMS
SEAASGNGGVTNGVAPAVGMPVATGGAAISPPVAAWENGLRTEVAKLLRENAADVMDELSRRFEAAVIREALDFTRGRKV
EAAERLGIGRNTITRKIQELNLEP
>Mature_504_residues
MKPIWIVDDDQSIRWVLEKALARENFATRSFANVREAAGALEHDSPQVLVSDIRMPGGSGLELLQTVRDRVPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVDESMRGEQTWDDRVAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQAMRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPALRERSEDIPLLTR
HFLQKSARDLGVEPKRVSDEALVYMTSLPFPGNVRQLENLANWLTVMAPAQTIEIKDLPPDLVSTQQSGAEHATSAVSMS
SEAASGNGGVTNGVAPAVGMPVATGGAAISPPVAAWENGLRTEVAKLLRENAADVMDELSRRFEAAVIREALDFTRGRKV
EAAERLGIGRNTITRKIQELNLEP

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=501, Percent_Identity=54.6906187624751, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1788550, Length=511, Percent_Identity=38.747553816047, Blast_Score=352, Evalue=4e-98,
Organism=Escherichia coli, GI1790437, Length=383, Percent_Identity=42.0365535248042, Blast_Score=291, Evalue=1e-79,
Organism=Escherichia coli, GI1788905, Length=370, Percent_Identity=41.6216216216216, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI87082117, Length=225, Percent_Identity=52.4444444444444, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1789087, Length=272, Percent_Identity=45.5882352941176, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1789233, Length=383, Percent_Identity=38.1201044386423, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=48.0851063829787, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1786524, Length=264, Percent_Identity=45.8333333333333, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI1787583, Length=251, Percent_Identity=41.8326693227092, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=44.7826086956522, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=35.8407079646018, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87081858, Length=240, Percent_Identity=32.9166666666667, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789809, Length=111, Percent_Identity=31.5315315315315, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55253; Mature: 55253

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIWIVDDDQSIRWVLEKALARENFATRSFANVREAAGALEHDSPQVLVSDIRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCC
LELLQTVRDRVPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVDES
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
MRGEQTWDDRVAEAPEMLGQAPAMQDMFRAIGRLSHSAATVLITGESGTGKELVARALHR
HCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
HSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQAMRQGRFEQAENGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCEEEHHHCC
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
IRLRLPALRERSEDIPLLTRHFLQKSARDLGVEPKRVSDEALVYMTSLPFPGNVRQLENL
HHHHCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
ANWLTVMAPAQTIEIKDLPPDLVSTQQSGAEHATSAVSMSSEAASGNGGVTNGVAPAVGM
HHHHHHHCCCCEEEECCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PVATGGAAISPPVAAWENGLRTEVAKLLRENAADVMDELSRRFEAAVIREALDFTRGRKV
CCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EAAERLGIGRNTITRKIQELNLEP
HHHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPIWIVDDDQSIRWVLEKALARENFATRSFANVREAAGALEHDSPQVLVSDIRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCC
LELLQTVRDRVPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVDES
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
MRGEQTWDDRVAEAPEMLGQAPAMQDMFRAIGRLSHSAATVLITGESGTGKELVARALHR
HCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
HSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQAMRQGRFEQAENGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCEEEHHHCC
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
IRLRLPALRERSEDIPLLTRHFLQKSARDLGVEPKRVSDEALVYMTSLPFPGNVRQLENL
HHHHCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
ANWLTVMAPAQTIEIKDLPPDLVSTQQSGAEHATSAVSMSSEAASGNGGVTNGVAPAVGM
HHHHHHHCCCCEEEECCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PVATGGAAISPPVAAWENGLRTEVAKLLRENAADVMDELSRRFEAAVIREALDFTRGRKV
CCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EAAERLGIGRNTITRKIQELNLEP
HHHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]