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The map label for this gene is trmJ [H]

Identifier: 186476154

GI number: 186476154

Start: 1568296

End: 1569126

Strand: Reverse

Name: trmJ [H]

Synonym: Bphy_1396

Alternate gene names: 186476154

Gene position: 1569126-1568296 (Counterclockwise)

Preceding gene: 186476159

Following gene: 186476153

Centisome position: 45.1

GC content: 65.82

Gene sequence:

>831_bases
ATGCTTATCGTGGTTCAGACGCCTTCCTTGACCTCCAGCAGTCCCGCTTCCGACAACTCCGGCACCATCGGCGGCGGCTT
CACGTCGACGCGTTTCGTACTCGTCGAGCCCAGTCATCCCGGCAACGTCGGCGCAGCGGCTCGCGCGCTGAAGACAATGG
GCTTCTCGCGGCTCGTGCTCGTGTCGCCGCGCGTGGCGGACGTGAAAAACGACCCGGAAGCGATCGCGATGGCCAGCGGC
GCGGACGACGTGCTTGCATCCGCGCATGTCGTGCCGACGCTCGCAGACGCGCTGTCGGGGGCCAGCTGGTCGCTGGCGCT
GACGGCGCGCGCCCGCGAATACGGGCCGCCGCAACTCGCGCCCCGCGCGGCCGCAACGCAGGCGCGCGAGCATGCCGCAC
ATGGTGATATCGCGCTCGTGTTCGGCAACGAGCGCACGGGTCTGTCGAACGACGATGTCGAGCGATGCAGCGCGCTGGCG
CATATTCCTGCCAACCCCGCCTACAGTTCGCTGAATCTGTCGCAGGCGATCCAGGTGCTGTCGTACGAGCTGCGGATGGC
ATATCTGGTCGAAGGCGACACCGCAGATCCCGCGTCGGGCAGCGCGGGCGCACTCGCTGCCAGCGACGAAATCGAGCGTA
TGTACGTGCATCTGGAAAACGCGCTGATCGCGCTCGACTTTCTGGACCCCGGCAATCCGAAGAAGCTGATGTCGCGGTTG
CGGCGTCTCTTTGCGCGTTCGGGCCTCGAGCGCGAAGAGGTCAATATTGTGCGTGGCATCGCGAAGCACATTCTGCTGAA
GGCGAAAGGGCCCGACGCCCACGAACGTTGA

Upstream 100 bases:

>100_bases
TGCCGGCGCGTCCGTGCCGGGGAAAAGGGTAGATGTTGGCCGAATGGCAGATGGAAAGCGCGAGCGGGCGGCGCTACCAT
GACTGTTTCGTTGCTATTCA

Downstream 100 bases:

>100_bases
GCGGCGCGTGTGCAGGTCGTGCGAGGCGGCCGTTTTGTTCACGAGACGCCTGACCTTGTCGCCCGCGTGGGCTTCAGGCA
GGTTTTGCGCATTCGCCCTA

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MLIVVQTPSLTSSSPASDNSGTIGGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVSPRVADVKNDPEAIAMASG
ADDVLASAHVVPTLADALSGASWSLALTARAREYGPPQLAPRAAATQAREHAAHGDIALVFGNERTGLSNDDVERCSALA
HIPANPAYSSLNLSQAIQVLSYELRMAYLVEGDTADPASGSAGALAASDEIERMYVHLENALIALDFLDPGNPKKLMSRL
RRLFARSGLEREEVNIVRGIAKHILLKAKGPDAHER

Sequences:

>Translated_276_residues
MLIVVQTPSLTSSSPASDNSGTIGGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVSPRVADVKNDPEAIAMASG
ADDVLASAHVVPTLADALSGASWSLALTARAREYGPPQLAPRAAATQAREHAAHGDIALVFGNERTGLSNDDVERCSALA
HIPANPAYSSLNLSQAIQVLSYELRMAYLVEGDTADPASGSAGALAASDEIERMYVHLENALIALDFLDPGNPKKLMSRL
RRLFARSGLEREEVNIVRGIAKHILLKAKGPDAHER
>Mature_276_residues
MLIVVQTPSLTSSSPASDNSGTIGGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVSPRVADVKNDPEAIAMASG
ADDVLASAHVVPTLADALSGASWSLALTARAREYGPPQLAPRAAATQAREHAAHGDIALVFGNERTGLSNDDVERCSALA
HIPANPAYSSLNLSQAIQVLSYELRMAYLVEGDTADPASGSAGALAASDEIERMYVHLENALIALDFLDPGNPKKLMSRL
RRLFARSGLEREEVNIVRGIAKHILLKAKGPDAHER

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=247, Percent_Identity=44.1295546558704, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1790865, Length=158, Percent_Identity=37.3417721518987, Blast_Score=91, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29035; Mature: 29035

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIVVQTPSLTSSSPASDNSGTIGGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVL
CEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEE
VSPRVADVKNDPEAIAMASGADDVLASAHVVPTLADALSGASWSLALTARAREYGPPQLA
ECCCHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHCCCCCCC
PRAAATQAREHAAHGDIALVFGNERTGLSNDDVERCSALAHIPANPAYSSLNLSQAIQVL
CHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
SYELRMAYLVEGDTADPASGSAGALAASDEIERMYVHLENALIALDFLDPGNPKKLMSRL
HHHEEEEEEEECCCCCCCCCCCCCEECHHHHHHHHHEECCEEEEEEECCCCCHHHHHHHH
RRLFARSGLEREEVNIVRGIAKHILLKAKGPDAHER
HHHHHHCCCCHHHHHHHHHHHHHHEEECCCCCCCCC
>Mature Secondary Structure
MLIVVQTPSLTSSSPASDNSGTIGGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVL
CEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEE
VSPRVADVKNDPEAIAMASGADDVLASAHVVPTLADALSGASWSLALTARAREYGPPQLA
ECCCHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHCCCCCCC
PRAAATQAREHAAHGDIALVFGNERTGLSNDDVERCSALAHIPANPAYSSLNLSQAIQVL
CHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
SYELRMAYLVEGDTADPASGSAGALAASDEIERMYVHLENALIALDFLDPGNPKKLMSRL
HHHEEEEEEEECCCCCCCCCCCCCEECHHHHHHHHHEECCEEEEEEECCCCCHHHHHHHH
RRLFARSGLEREEVNIVRGIAKHILLKAKGPDAHER
HHHHHHCCCCHHHHHHHHHHHHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA