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The map label for this gene is mls [H]

Identifier: 186476130

GI number: 186476130

Start: 1540300

End: 1541892

Strand: Direct

Name: mls [H]

Synonym: Bphy_1372

Alternate gene names: 186476130

Gene position: 1540300-1541892 (Clockwise)

Preceding gene: 186476129

Following gene: 186476133

Centisome position: 44.27

GC content: 62.71

Gene sequence:

>1593_bases
ATGGCGAACACGTTGTCGCTCCCGCAGGGCATGAACATCACCGGCGAAATCAAGCCCGGCTTCGAGGCGATTCTGACGCC
CCAGGCACTCGAGCTCGTCGCCGCCCTGCACCGGACATTCGAGCCGCGTCGCCAGCAACTGCTGAAACTGCGCGCCGAAC
GCGCGCAGCGTCTCGACGCCGGCGAGCGCCCGGATTTTCTCGCCGGGACGCGAGCCATCCGCGAAGGCGACTGGACCATC
GCGCCGCTGCCGCACGATCTGCAGTGCCGCCGCGTCGAGATCACGGGCCCCATCGAGCGGAAGATGATCATCAACGCGCT
GAACTCGGGCGCAGATTCGTACATGACCGACTTCGAGGATTCGAATGCGCCGAGCTGGGACAACCAGATCACCGGACACA
TCAACCTGAAGGAAGCGGTGCGCCGCACGATCTCGCTGGAGCAGAACGGCAAGTCGTACAGGCTCAACGACAGGATTGCG
ACGCTGATCGTGCGTCCGCGCGGCTGGCATCTGGATGAGAAGCACGTCACCGTCGACGGACAACGGGTATCGGGCGGCAT
CTTCGACTTCGCGCTGTTCCTGCATCACAACGCGAAAGAGCAACGCTCGCGCGGCACGGGTCCCTACTTTTACCTGCCGA
AGATGGAGAGCCATCTGGAAGCGCGTCTGTGGAACGACATCTTCGTCGCGGCGCAGGAAGCGGTTGGCGTGCCGCGCGGC
ACCATCCGCGCAACCGTGTTGATCGAGACGATCCTGGCTGCGTTCGAAATGGATGAGATCCTGTACGAACTGCGTGAACA
CAGCTCAGGCCTGAACGCCGGACGTTGGGACTACATCTTTTCGGCGATCAAGAAGTTCAAGAACGACAAAGATTTTTGCC
TCGCGGATCGCTCGCAGATCACGATGACGGTGCCGTTCATGCGCGCATACGCGCTGCAGCTGCTGAAGACATGCCACCGA
CGCAACGCGCCAGCGATCGGCGGCATGAGCGCGCTGATCCCGATCAAGAGCGACGCGGCAGCCAACGACAAAGCGATGAC
CGGCGTGCGCTCCGACAAGGCACGCGATGCGACGGATGGTTACGACGGCGGCTGGGTCGCGCATCCCGGTCTCGTGCCCA
TCGCGATGGAAGAATTCGTGAAGGTACTGGGCGACAAGCCGAATCAGATCGGCAAGCAGCGCGACGATGTGCAGGTGACA
GGCAAGGATCTGCTCGACTTCCGCCCGGAAGCGCCCATCACCGAGGCGGGGTTGCGCAACAACATCAACGTCGGCATTCA
CTACCTGGGTTCGTGGCTCGCAGGCAATGGCTGCGTGCCGATTCACAACCTGATGGAAGATGCCGCGACGGCGGAAATCT
CGCGCTCCCAGGTGTGGCAATGGATCCGCTCGCCGAAGGGCAAACTCGACGACGGCCGCAAGGTAACGGCCGAACTGGTG
CGCGAGCTGACGGGGCAGGAACTCGACAAGGTGAAGGCCGCGATCGCGGGCGACACCGCGCCGTACGTTCGCGCCGCGCA
GATCTTCGTCGACATGTCGACGTCGGAGACCTTCACCGAGTTTCTGACGCTGCCGCTTTACGAAGAACTCTGA

Upstream 100 bases:

>100_bases
CGCACGCGCCACGGCCCTGGCGCATGACGGCATCGGCCCGACTGACCACATCGCAATCGCGTCACCCGCATTGTTGAAAC
TGACCGACCAAGGAGAAATC

Downstream 100 bases:

>100_bases
ACGACGCGATGCGCGTGCGTGTCCTGGCCGTGATGCTTGCTGGCGGGCGCCGCCTACGGCGACGCACAGGTGCTGCTTTC
AACGGTTGACCGACCGGGAT

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MANTLSLPQGMNITGEIKPGFEAILTPQALELVAALHRTFEPRRQQLLKLRAERAQRLDAGERPDFLAGTRAIREGDWTI
APLPHDLQCRRVEITGPIERKMIINALNSGADSYMTDFEDSNAPSWDNQITGHINLKEAVRRTISLEQNGKSYRLNDRIA
TLIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLHHNAKEQRSRGTGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRG
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDKDFCLADRSQITMTVPFMRAYALQLLKTCHR
RNAPAIGGMSALIPIKSDAAANDKAMTGVRSDKARDATDGYDGGWVAHPGLVPIAMEEFVKVLGDKPNQIGKQRDDVQVT
GKDLLDFRPEAPITEAGLRNNINVGIHYLGSWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLDDGRKVTAELV
RELTGQELDKVKAAIAGDTAPYVRAAQIFVDMSTSETFTEFLTLPLYEEL

Sequences:

>Translated_530_residues
MANTLSLPQGMNITGEIKPGFEAILTPQALELVAALHRTFEPRRQQLLKLRAERAQRLDAGERPDFLAGTRAIREGDWTI
APLPHDLQCRRVEITGPIERKMIINALNSGADSYMTDFEDSNAPSWDNQITGHINLKEAVRRTISLEQNGKSYRLNDRIA
TLIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLHHNAKEQRSRGTGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRG
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDKDFCLADRSQITMTVPFMRAYALQLLKTCHR
RNAPAIGGMSALIPIKSDAAANDKAMTGVRSDKARDATDGYDGGWVAHPGLVPIAMEEFVKVLGDKPNQIGKQRDDVQVT
GKDLLDFRPEAPITEAGLRNNINVGIHYLGSWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLDDGRKVTAELV
RELTGQELDKVKAAIAGDTAPYVRAAQIFVDMSTSETFTEFLTLPLYEEL
>Mature_529_residues
ANTLSLPQGMNITGEIKPGFEAILTPQALELVAALHRTFEPRRQQLLKLRAERAQRLDAGERPDFLAGTRAIREGDWTIA
PLPHDLQCRRVEITGPIERKMIINALNSGADSYMTDFEDSNAPSWDNQITGHINLKEAVRRTISLEQNGKSYRLNDRIAT
LIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLHHNAKEQRSRGTGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRGT
IRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDKDFCLADRSQITMTVPFMRAYALQLLKTCHRR
NAPAIGGMSALIPIKSDAAANDKAMTGVRSDKARDATDGYDGGWVAHPGLVPIAMEEFVKVLGDKPNQIGKQRDDVQVTG
KDLLDFRPEAPITEAGLRNNINVGIHYLGSWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLDDGRKVTAELVR
ELTGQELDKVKAAIAGDTAPYVRAAQIFVDMSTSETFTEFLTLPLYEEL

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=516, Percent_Identity=47.8682170542636, Blast_Score=483, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17561814, Length=526, Percent_Identity=50.3802281368821, Blast_Score=512, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI71982926, Length=420, Percent_Identity=51.9047619047619, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6324212, Length=514, Percent_Identity=51.9455252918288, Blast_Score=537, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6322222, Length=514, Percent_Identity=50.9727626459144, Blast_Score=516, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 59181; Mature: 59050

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTLSLPQGMNITGEIKPGFEAILTPQALELVAALHRTFEPRRQQLLKLRAERAQRLDA
CCCCCCCCCCCCEEECCCCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
GERPDFLAGTRAIREGDWTIAPLPHDLQCRRVEITGPIERKMIINALNSGADSYMTDFED
CCCCCHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHCCCC
SNAPSWDNQITGHINLKEAVRRTISLEQNGKSYRLNDRIATLIVRPRGWHLDEKHVTVDG
CCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCEEECCEEEEEEECCCCCCCCCCEEEECC
QRVSGGIFDFALFLHHNAKEQRSRGTGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRG
CCCCCHHHHHHHHHHCCCHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCC
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDKDFCLADRSQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCE
TMTVPFMRAYALQLLKTCHRRNAPAIGGMSALIPIKSDAAANDKAMTGVRSDKARDATDG
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHCCCCCCCCCCCCCC
YDGGWVAHPGLVPIAMEEFVKVLGDKPNQIGKQRDDVQVTGKDLLDFRPEAPITEAGLRN
CCCCEEECCCCCHHHHHHHHHHHCCCCHHHCCCCCCEEECCCHHHCCCCCCCCCHHCCCC
NINVGIHYLGSWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLDDGRKVTAELV
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
RELTGQELDKVKAAIAGDTAPYVRAAQIFVDMSTSETFTEFLTLPLYEEL
HHHHHHHHHHHHHHHCCCCCHHHEEEEEEEECCCHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
ANTLSLPQGMNITGEIKPGFEAILTPQALELVAALHRTFEPRRQQLLKLRAERAQRLDA
CCCCCCCCCCCEEECCCCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
GERPDFLAGTRAIREGDWTIAPLPHDLQCRRVEITGPIERKMIINALNSGADSYMTDFED
CCCCCHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHCCCC
SNAPSWDNQITGHINLKEAVRRTISLEQNGKSYRLNDRIATLIVRPRGWHLDEKHVTVDG
CCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCEEECCEEEEEEECCCCCCCCCCEEEECC
QRVSGGIFDFALFLHHNAKEQRSRGTGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRG
CCCCCHHHHHHHHHHCCCHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCC
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDKDFCLADRSQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCE
TMTVPFMRAYALQLLKTCHRRNAPAIGGMSALIPIKSDAAANDKAMTGVRSDKARDATDG
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHCCCCCCCCCCCCCC
YDGGWVAHPGLVPIAMEEFVKVLGDKPNQIGKQRDDVQVTGKDLLDFRPEAPITEAGLRN
CCCCEEECCCCCHHHHHHHHHHHCCCCHHHCCCCCCEEECCCHHHCCCCCCCCCHHCCCC
NINVGIHYLGSWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLDDGRKVTAELV
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
RELTGQELDKVKAAIAGDTAPYVRAAQIFVDMSTSETFTEFLTLPLYEEL
HHHHHHHHHHHHHHHCCCCCHHHEEEEEEEECCCHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA