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The map label for this gene is dmlR [H]

Identifier: 186476128

GI number: 186476128

Start: 1538381

End: 1539283

Strand: Reverse

Name: dmlR [H]

Synonym: Bphy_1370

Alternate gene names: 186476128

Gene position: 1539283-1538381 (Counterclockwise)

Preceding gene: 186476131

Following gene: 186476127

Centisome position: 44.24

GC content: 65.34

Gene sequence:

>903_bases
ATGGACCGTTTCAAGCAGATCGAGACCTTCGTGACCGTCGCGGCGCGCGGCAGCCTGTCGGCGGCAGCGCAGGCGGAAGG
CGTAGCGCCTGCCATCATCGGGCGCCGTATCGACGCGCTCGAGGAACGCCTCGGCGTCAAGCTGCTCGTGCGCACCACGC
GCCGCATCACATTGACCTACGAAGGCTCCGCCTTTCTCGAAGACTGTCAGCGGATCATTCACGACATGCAGAACGCCGAA
GCGAGCGTGTCGGCGGGCGGCGTCAAGGCGAGCGGGCATTTGCGCTTGTCGGCGCCAGCGGGCTTCGGCCGCAGACACGT
CGCACCGCTCGTGCCTGCGTTCACGGTGGCGCATCCCGACGTGTCGATTTCTCTCGACCTGTCCGACCGCATGGTCGACC
TCGTCAACGAAGGATTCGATTGCGCGATCCGGCTCGGCGAACTTCCGGATTCGTCGCTGGTGTCGTTGAAGCTCGGTGAG
AACCGGCGTGTCTGTGTCGCGTCGCCGGCCTATCTCGCGCGACGCGCGGCGCCGCAGACGCTCGCCGATCTCGCGCACCA
TAACTGTCTCGCGCTTGGCGCGAGTGCGAACCAGCAGCGCGGCTGGGTGTTTCAGCAGGGTGACAAGGTCGTGACGATCA
AGGTATCGGGCACGATGGAATGCTCGGACGGAGCGGTGCTGCACGAGTGGTGCCTGGAAGGCTACGGGCTTGCATGGCGC
TCGTGGTGGGAAGTGGGCGCGGACATCGCGGCGGGAAGCCTCGTCAGCGTGCTCGACAACCATGCGGCGCCGCCCATCGG
CATTCACGCCGTGTTTCCGCAGCGGCGGCATTTGCCGTTGCGCGTGCGTCTTTTCCTCGATTTTCTCAAGCATACCTACG
GCAACCCGGCGTACTGGATCTGA

Upstream 100 bases:

>100_bases
AAAAAAGACGGTAAAGATCACTTGATCTTTTACTTTCATCAACCTAATCTAACGCGCATCTCCTTTTTTGTGCGCGACCG
ATGCGCCCGCTCACCCGCAG

Downstream 100 bases:

>100_bases
CGGCGGCGGCTCGGACCGCGCACGGGTTTCCGATATGCGGACATGCCCGCAGGCAAGGCTTGCACGACATTCCGGCGCAC
TACAATCGTTCTGACAGCCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDRFKQIETFVTVAARGSLSAAAQAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTYEGSAFLEDCQRIIHDMQNAE
ASVSAGGVKASGHLRLSAPAGFGRRHVAPLVPAFTVAHPDVSISLDLSDRMVDLVNEGFDCAIRLGELPDSSLVSLKLGE
NRRVCVASPAYLARRAAPQTLADLAHHNCLALGASANQQRGWVFQQGDKVVTIKVSGTMECSDGAVLHEWCLEGYGLAWR
SWWEVGADIAAGSLVSVLDNHAAPPIGIHAVFPQRRHLPLRVRLFLDFLKHTYGNPAYWI

Sequences:

>Translated_300_residues
MDRFKQIETFVTVAARGSLSAAAQAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTYEGSAFLEDCQRIIHDMQNAE
ASVSAGGVKASGHLRLSAPAGFGRRHVAPLVPAFTVAHPDVSISLDLSDRMVDLVNEGFDCAIRLGELPDSSLVSLKLGE
NRRVCVASPAYLARRAAPQTLADLAHHNCLALGASANQQRGWVFQQGDKVVTIKVSGTMECSDGAVLHEWCLEGYGLAWR
SWWEVGADIAAGSLVSVLDNHAAPPIGIHAVFPQRRHLPLRVRLFLDFLKHTYGNPAYWI
>Mature_300_residues
MDRFKQIETFVTVAARGSLSAAAQAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTYEGSAFLEDCQRIIHDMQNAE
ASVSAGGVKASGHLRLSAPAGFGRRHVAPLVPAFTVAHPDVSISLDLSDRMVDLVNEGFDCAIRLGELPDSSLVSLKLGE
NRRVCVASPAYLARRAAPQTLADLAHHNCLALGASANQQRGWVFQQGDKVVTIKVSGTMECSDGAVLHEWCLEGYGLAWR
SWWEVGADIAAGSLVSVLDNHAAPPIGIHAVFPQRRHLPLRVRLFLDFLKHTYGNPAYWI

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=37.4570446735395, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=32.1678321678322, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=32.9931972789116, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=31.3793103448276, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1787589, Length=287, Percent_Identity=29.2682926829268, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=28.6219081272085, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=28.6689419795222, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1786448, Length=274, Percent_Identity=27.3722627737226, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788706, Length=191, Percent_Identity=28.2722513089005, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790011, Length=297, Percent_Identity=23.2323232323232, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=26.3779527559055, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=25.3424657534247, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790208, Length=175, Percent_Identity=30.2857142857143, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32604; Mature: 32604

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRFKQIETFVTVAARGSLSAAAQAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTY
CCHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEECEEEEEEE
EGSAFLEDCQRIIHDMQNAEASVSAGGVKASGHLRLSAPAGFGRRHVAPLVPAFTVAHPD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCEEEEECCC
VSISLDLSDRMVDLVNEGFDCAIRLGELPDSSLVSLKLGENRRVCVASPAYLARRAAPQT
EEEEEECHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECHHHHHHHCCHHH
LADLAHHNCLALGASANQQRGWVFQQGDKVVTIKVSGTMECSDGAVLHEWCLEGYGLAWR
HHHHHCCCEEEECCCCCCCCCEEEECCCEEEEEEEECEEECCCCCHHHHHHHCCCCCHHH
SWWEVGADIAAGSLVSVLDNHAAPPIGIHAVFPQRRHLPLRVRLFLDFLKHTYGNPAYWI
HHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDRFKQIETFVTVAARGSLSAAAQAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTY
CCHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEECEEEEEEE
EGSAFLEDCQRIIHDMQNAEASVSAGGVKASGHLRLSAPAGFGRRHVAPLVPAFTVAHPD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCEEEEECCC
VSISLDLSDRMVDLVNEGFDCAIRLGELPDSSLVSLKLGENRRVCVASPAYLARRAAPQT
EEEEEECHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECHHHHHHHCCHHH
LADLAHHNCLALGASANQQRGWVFQQGDKVVTIKVSGTMECSDGAVLHEWCLEGYGLAWR
HHHHHCCCEEEECCCCCCCCCEEEECCCEEEEEEEECEEECCCCCHHHHHHHCCCCCHHH
SWWEVGADIAAGSLVSVLDNHAAPPIGIHAVFPQRRHLPLRVRLFLDFLKHTYGNPAYWI
HHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]