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The map label for this gene is thiD [H]

Identifier: 186476119

GI number: 186476119

Start: 1529176

End: 1529982

Strand: Direct

Name: thiD [H]

Synonym: Bphy_1361

Alternate gene names: 186476119

Gene position: 1529176-1529982 (Clockwise)

Preceding gene: 186476118

Following gene: 186476124

Centisome position: 43.95

GC content: 67.04

Gene sequence:

>807_bases
ATGACCCGACCGATTCCCAACGTCCTGACGATCGCCGGTTCCGATTCCGGCGGCGGCGCCGGCATCCAGGCCGACCTCAA
GACATTCTCCGCGCTCGGCGCGTATGGCGCGAGCGTGATCACCGCATTGACGGCGCAGAACACGCGCGGCGTGACGGCCA
TCCACACACCCGAGCCCGCGTTCGTGAGCGCGCAGCTCGACGCCGTGTTCGACGACATCCGCATCGACGCGGTGAAGATC
GGCATGCTGGCGAATGCGTCGATCGTGCTCGCCGTCGCGGATGCGTTGCGCCGCCACAAGCCGAAGCATGTCGTGCTCGA
CACGGTGATGATCTCGAAGAGCAATCATGCGCTGCTCGCGCCCGAAGCCGTCGATGCCGTGCGCAGCGAACTGTTGCCGC
TCGCCGATCTGCTGACGCCGAATCTGCCGGAAGCGGCCGCGCTGCTCGGCACGTCGAGCGCGCGGGACGAAGCCGCGATG
GTCGATCAGGGTGAAGCCCTGCGCAAGCTGGGCGCACGCGCGGTGCTGATGAAAGGCGGCCATCTCGCCGCCACCGACAG
CCCCGACTGGCTGATCCAGGAAAGCGGCACGATGCGCCTCGCCGGTCCGCGCGTGCCCGTCAAGAACACGCATGGGACGG
GGTGCTCGTTGTCGTCGGCTATCGCGGCACTGATTCCGCAGCACAACGATCTGGCAAGCGCCGTCGCGGATGCCAAGCAA
TGGCTGACGGGCGCACTCGAGCAGAGCGGACGACTCGACGTCGGGCACGGCGTCGGGCCCGTGCATCATTTCTATCGTTG
GTGGTAA

Upstream 100 bases:

>100_bases
ATCGGCGAAGCGCGGCACTAACGCGCGCACGCACTGCCGCGCCCTCTCTTCGCAATCTTCTGTGCGCGCAGCATGCGCGC
CCCGCTTTGCAGGCTTTGCC

Downstream 100 bases:

>100_bases
CGCGTCGCTTCGGCGCGAGCGGGTGCCTCTAGGGACGGCGCGTCGACTGACGTGCAAACGCGTGAGCCTGCTCAGGCCAG
TCGTCGGGCACGATAAAGCC

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTRPIPNVLTIAGSDSGGGAGIQADLKTFSALGAYGASVITALTAQNTRGVTAIHTPEPAFVSAQLDAVFDDIRIDAVKI
GMLANASIVLAVADALRRHKPKHVVLDTVMISKSNHALLAPEAVDAVRSELLPLADLLTPNLPEAAALLGTSSARDEAAM
VDQGEALRKLGARAVLMKGGHLAATDSPDWLIQESGTMRLAGPRVPVKNTHGTGCSLSSAIAALIPQHNDLASAVADAKQ
WLTGALEQSGRLDVGHGVGPVHHFYRWW

Sequences:

>Translated_268_residues
MTRPIPNVLTIAGSDSGGGAGIQADLKTFSALGAYGASVITALTAQNTRGVTAIHTPEPAFVSAQLDAVFDDIRIDAVKI
GMLANASIVLAVADALRRHKPKHVVLDTVMISKSNHALLAPEAVDAVRSELLPLADLLTPNLPEAAALLGTSSARDEAAM
VDQGEALRKLGARAVLMKGGHLAATDSPDWLIQESGTMRLAGPRVPVKNTHGTGCSLSSAIAALIPQHNDLASAVADAKQ
WLTGALEQSGRLDVGHGVGPVHHFYRWW
>Mature_267_residues
TRPIPNVLTIAGSDSGGGAGIQADLKTFSALGAYGASVITALTAQNTRGVTAIHTPEPAFVSAQLDAVFDDIRIDAVKIG
MLANASIVLAVADALRRHKPKHVVLDTVMISKSNHALLAPEAVDAVRSELLPLADLLTPNLPEAAALLGTSSARDEAAMV
DQGEALRKLGARAVLMKGGHLAATDSPDWLIQESGTMRLAGPRVPVKNTHGTGCSLSSAIAALIPQHNDLASAVADAKQW
LTGALEQSGRLDVGHGVGPVHHFYRWW

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=262, Percent_Identity=61.4503816793893, Blast_Score=329, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6325378, Length=277, Percent_Identity=31.7689530685921, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324997, Length=276, Percent_Identity=32.6086956521739, Blast_Score=115, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6324517, Length=276, Percent_Identity=31.5217391304348, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27911; Mature: 27780

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPIPNVLTIAGSDSGGGAGIQADLKTFSALGAYGASVITALTAQNTRGVTAIHTPEPA
CCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
FVSAQLDAVFDDIRIDAVKIGMLANASIVLAVADALRRHKPKHVVLDTVMISKSNHALLA
EEHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEC
PEAVDAVRSELLPLADLLTPNLPEAAALLGTSSARDEAAMVDQGEALRKLGARAVLMKGG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHCCCHHHHHHCCEEEEEECC
HLAATDSPDWLIQESGTMRLAGPRVPVKNTHGTGCSLSSAIAALIPQHNDLASAVADAKQ
EEEECCCCCEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
WLTGALEQSGRLDVGHGVGPVHHFYRWW
HHHHHHHHCCCEECCCCCCHHHHHHHCC
>Mature Secondary Structure 
TRPIPNVLTIAGSDSGGGAGIQADLKTFSALGAYGASVITALTAQNTRGVTAIHTPEPA
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
FVSAQLDAVFDDIRIDAVKIGMLANASIVLAVADALRRHKPKHVVLDTVMISKSNHALLA
EEHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEC
PEAVDAVRSELLPLADLLTPNLPEAAALLGTSSARDEAAMVDQGEALRKLGARAVLMKGG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHCCCHHHHHHCCEEEEEECC
HLAATDSPDWLIQESGTMRLAGPRVPVKNTHGTGCSLSSAIAALIPQHNDLASAVADAKQ
EEEECCCCCEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
WLTGALEQSGRLDVGHGVGPVHHFYRWW
HHHHHHHHCCCEECCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]