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The map label for this gene is rluF [H]

Identifier: 186476099

GI number: 186476099

Start: 1502266

End: 1504140

Strand: Direct

Name: rluF [H]

Synonym: Bphy_1341

Alternate gene names: 186476099

Gene position: 1502266-1504140 (Clockwise)

Preceding gene: 186476098

Following gene: 186476102

Centisome position: 43.18

GC content: 69.92

Gene sequence:

>1875_bases
ATGCGAGTCAAACTGACAGCCAAGCATCCGCGGCCGGCTTCGTCCGAACGCGCCCCTGTGCGTTCGGGCAGCACGACTGC
CCGCAAGCCGACTCGGCCGGCCGCCCCGGCAGACAAACCCGCTGCGCAGAGGAAGCCGCGCAGCGACGCCGGTTCGGCGG
CGGGCGCACCCGCTGCACGCCGTGGCACCGCTTCGGGCGAAGGGACAAAGCGCCCGGCCACGAGGAGCAACGCGGCCGGC
GCGCCGGGGGAGCGGCGCGGCCCGTCGACGGGCGGGCGCAACGGCGCGCCCGCTCGCTCCGGCGACCGTCCGGCGCGCGC
CGCGGGCACGGGCTCACGCGAGCGCGGCCTTGCCGAACGTGGCGCTTCCGCTTCGTTCGATCGCCGTCCGCCGGGCGATC
GGCCGGCACGCAAGGAAGGCGCTTCCGCCGGCACGGGTTTCCGTCGGGACGGGGACGCGCGAACCAACGCGCGCACTTCG
TCAGATCGTCCGTCGCGCAGCGGCGCGGCGTCGCGTGCTGGCGAACGCGCACCGCGCTTCTCCCGTGACGACGATGCACG
GGTCGCACGACGTACGGCGCCCGACAGCGATGACGGCCGGCGCGCTCCGCGCCCGCCGCGAGAGAGCGATGACGTTCGCC
GCGCGCCGCGCACGGCGCGTACCGAAGGTACGCCTTCGCGCGAACGCACGGAACGACGCTACCGTGACGACGCCCCCGAA
GGCCGCACGACGCGCTCGCCGCGCGGCGAGCGATCCGAAGCACGCGGTGAACGCAGCTATGCGCGCCCGGCCAGGGCTGC
CTACGAAGATCGCGGCGCAGCGCCGGAACGCCGCCCACGCGGCGAAGGTGCACCGCGCCGCAGCCGCGAAGACGTCGCAG
AAGGTCGCAGCGAACGCACTTACGCAAAGCCCGTGAAGGCCGTCTACGCGGACCGCGCCAGCGACGCTCCGCCGAAGGCC
GCACCGCGCGCCACGAAGACCAAACGCGACACCCGCTCGGTCGAAGACAGCTTTGACCGTCCGGCGCGCTTCGACCGCCC
CGCTGAAAAGACGTCACGCAAGCCCCGCCCGGCCGCCGCGTCGCGTCAACGCGACGAAGGCGAATACAACGACGCCCCCG
GCAATCTGCGCCTGTCGAAACTGATGTCCGAACTCGGCCTGTGCTCGCGCCGCGAAGCCGACGAATGGATCGAAAAAGGC
TGGGTTACCGTCGACGGCGTCGTCGTCGATAAGCTGGGCACGAAGGTCCGCCCCGACGCCGATATCCAGATCGACCCGGC
CGCTCAGGCAGCCCAACAGAAACAGGTGACGATCCTCGTGCACAAGCCGGTCGGCTATGTGTCGGGTCAGGCGGAAGATG
GCTACGAGCCTGCCGTGACGCTCGTCAAACCGGAGAACCGCTGGGACGGCGACCGTACTGATATCCGTTTCTCGGCTGTC
CATTTGCGACAACTGGCGCCTGCGGGACGCCTCGATATCGATTCGACGGGGCTGCTCGTGCTGACTCAGGACGGCCGCAT
CGCGAAGCAGCTGATCGGCGGGCACTCGGAGATCGACAAGGAGTATCTGGTGCGCGTCAGCTACGGCGACATCGAAACGG
ACGTCGAGCATGAGTTCCCCGCCGAGAGTCTGGCCAAACTGCGCCACGGCCTCGAACTCGACGGCGTGCCGCTCAAACCC
GCTATGGTCAGCTGGCAAAACGGCGAGCAATTGCGCTTCGTGCTGCGTGAAGGCAAGAAACGCCAGATTCGCCGGATGTG
CGAACTGGTCGGGCTGCATGTCGTGGGCCTCAAGCGCGTGCGGATGGGCCACGTGATGCTCGGCGCCTTGCCGCCCGGAC
AGTGGCGCTACCTCGGTCCGGACGAGTCGTTCTAA

Upstream 100 bases:

>100_bases
CGAGGACGTGTTCCTGCTCGACGGACACGGCCTCTCGGACAACCGACGACAAATCCAGGTTGAAACGGAGCTGCTGCGCG
CGATCGCAGTGTGAGATTTC

Downstream 100 bases:

>100_bases
GCGCGAACAAGCGCTGTCTTATGCAAACAAAAAGCCCGCATCCGCGGGCTTTTCTTATATCGAAGTTCGTAGATCAATCG
TCCGCTTTCGGATCGAGGTC

Product: RNA-binding S4 domain-containing protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MRVKLTAKHPRPASSERAPVRSGSTTARKPTRPAAPADKPAAQRKPRSDAGSAAGAPAARRGTASGEGTKRPATRSNAAG
APGERRGPSTGGRNGAPARSGDRPARAAGTGSRERGLAERGASASFDRRPPGDRPARKEGASAGTGFRRDGDARTNARTS
SDRPSRSGAASRAGERAPRFSRDDDARVARRTAPDSDDGRRAPRPPRESDDVRRAPRTARTEGTPSRERTERRYRDDAPE
GRTTRSPRGERSEARGERSYARPARAAYEDRGAAPERRPRGEGAPRRSREDVAEGRSERTYAKPVKAVYADRASDAPPKA
APRATKTKRDTRSVEDSFDRPARFDRPAEKTSRKPRPAAASRQRDEGEYNDAPGNLRLSKLMSELGLCSRREADEWIEKG
WVTVDGVVVDKLGTKVRPDADIQIDPAAQAAQQKQVTILVHKPVGYVSGQAEDGYEPAVTLVKPENRWDGDRTDIRFSAV
HLRQLAPAGRLDIDSTGLLVLTQDGRIAKQLIGGHSEIDKEYLVRVSYGDIETDVEHEFPAESLAKLRHGLELDGVPLKP
AMVSWQNGEQLRFVLREGKKRQIRRMCELVGLHVVGLKRVRMGHVMLGALPPGQWRYLGPDESF

Sequences:

>Translated_624_residues
MRVKLTAKHPRPASSERAPVRSGSTTARKPTRPAAPADKPAAQRKPRSDAGSAAGAPAARRGTASGEGTKRPATRSNAAG
APGERRGPSTGGRNGAPARSGDRPARAAGTGSRERGLAERGASASFDRRPPGDRPARKEGASAGTGFRRDGDARTNARTS
SDRPSRSGAASRAGERAPRFSRDDDARVARRTAPDSDDGRRAPRPPRESDDVRRAPRTARTEGTPSRERTERRYRDDAPE
GRTTRSPRGERSEARGERSYARPARAAYEDRGAAPERRPRGEGAPRRSREDVAEGRSERTYAKPVKAVYADRASDAPPKA
APRATKTKRDTRSVEDSFDRPARFDRPAEKTSRKPRPAAASRQRDEGEYNDAPGNLRLSKLMSELGLCSRREADEWIEKG
WVTVDGVVVDKLGTKVRPDADIQIDPAAQAAQQKQVTILVHKPVGYVSGQAEDGYEPAVTLVKPENRWDGDRTDIRFSAV
HLRQLAPAGRLDIDSTGLLVLTQDGRIAKQLIGGHSEIDKEYLVRVSYGDIETDVEHEFPAESLAKLRHGLELDGVPLKP
AMVSWQNGEQLRFVLREGKKRQIRRMCELVGLHVVGLKRVRMGHVMLGALPPGQWRYLGPDESF
>Mature_624_residues
MRVKLTAKHPRPASSERAPVRSGSTTARKPTRPAAPADKPAAQRKPRSDAGSAAGAPAARRGTASGEGTKRPATRSNAAG
APGERRGPSTGGRNGAPARSGDRPARAAGTGSRERGLAERGASASFDRRPPGDRPARKEGASAGTGFRRDGDARTNARTS
SDRPSRSGAASRAGERAPRFSRDDDARVARRTAPDSDDGRRAPRPPRESDDVRRAPRTARTEGTPSRERTERRYRDDAPE
GRTTRSPRGERSEARGERSYARPARAAYEDRGAAPERRPRGEGAPRRSREDVAEGRSERTYAKPVKAVYADRASDAPPKA
APRATKTKRDTRSVEDSFDRPARFDRPAEKTSRKPRPAAASRQRDEGEYNDAPGNLRLSKLMSELGLCSRREADEWIEKG
WVTVDGVVVDKLGTKVRPDADIQIDPAAQAAQQKQVTILVHKPVGYVSGQAEDGYEPAVTLVKPENRWDGDRTDIRFSAV
HLRQLAPAGRLDIDSTGLLVLTQDGRIAKQLIGGHSEIDKEYLVRVSYGDIETDVEHEFPAESLAKLRHGLELDGVPLKP
AMVSWQNGEQLRFVLREGKKRQIRRMCELVGLHVVGLKRVRMGHVMLGALPPGQWRYLGPDESF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=249, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1788510, Length=250, Percent_Identity=31.2, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787524, Length=253, Percent_Identity=32.0158102766798, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 68185; Mature: 68185

Theoretical pI: Translated: 11.20; Mature: 11.20

Prosite motif: PS50889 S4 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVKLTAKHPRPASSERAPVRSGSTTARKPTRPAAPADKPAAQRKPRSDAGSAAGAPAAR
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHH
RGTASGEGTKRPATRSNAAGAPGERRGPSTGGRNGAPARSGDRPARAAGTGSRERGLAER
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHH
GASASFDRRPPGDRPARKEGASAGTGFRRDGDARTNARTSSDRPSRSGAASRAGERAPRF
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
SRDDDARVARRTAPDSDDGRRAPRPPRESDDVRRAPRTARTEGTPSRERTERRYRDDAPE
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCC
GRTTRSPRGERSEARGERSYARPARAAYEDRGAAPERRPRGEGAPRRSREDVAEGRSERT
CCCCCCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCHH
YAKPVKAVYADRASDAPPKAAPRATKTKRDTRSVEDSFDRPARFDRPAEKTSRKPRPAAA
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHH
SRQRDEGEYNDAPGNLRLSKLMSELGLCSRREADEWIEKGWVTVDGVVVDKLGTKVRPDA
HCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCHHHHHHHCCCEEECCEEEECCCCCCCCCC
DIQIDPAAQAAQQKQVTILVHKPVGYVSGQAEDGYEPAVTLVKPENRWDGDRTDIRFSAV
CEEECHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEHHHH
HLRQLAPAGRLDIDSTGLLVLTQDGRIAKQLIGGHSEIDKEYLVRVSYGDIETDVEHEFP
HHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHCCHHHCCHHHEEEEECCCCCCCCCCCCC
AESLAKLRHGLELDGVPLKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLHVVGLKRV
HHHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RMGHVMLGALPPGQWRYLGPDESF
HHHHEEEECCCCCCEEECCCCCCC
>Mature Secondary Structure
MRVKLTAKHPRPASSERAPVRSGSTTARKPTRPAAPADKPAAQRKPRSDAGSAAGAPAAR
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHH
RGTASGEGTKRPATRSNAAGAPGERRGPSTGGRNGAPARSGDRPARAAGTGSRERGLAER
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHH
GASASFDRRPPGDRPARKEGASAGTGFRRDGDARTNARTSSDRPSRSGAASRAGERAPRF
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
SRDDDARVARRTAPDSDDGRRAPRPPRESDDVRRAPRTARTEGTPSRERTERRYRDDAPE
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCC
GRTTRSPRGERSEARGERSYARPARAAYEDRGAAPERRPRGEGAPRRSREDVAEGRSERT
CCCCCCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCHH
YAKPVKAVYADRASDAPPKAAPRATKTKRDTRSVEDSFDRPARFDRPAEKTSRKPRPAAA
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHH
SRQRDEGEYNDAPGNLRLSKLMSELGLCSRREADEWIEKGWVTVDGVVVDKLGTKVRPDA
HCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCHHHHHHHCCCEEECCEEEECCCCCCCCCC
DIQIDPAAQAAQQKQVTILVHKPVGYVSGQAEDGYEPAVTLVKPENRWDGDRTDIRFSAV
CEEECHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEHHHH
HLRQLAPAGRLDIDSTGLLVLTQDGRIAKQLIGGHSEIDKEYLVRVSYGDIETDVEHEFP
HHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHCCHHHCCHHHEEEEECCCCCCCCCCCCC
AESLAKLRHGLELDGVPLKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLHVVGLKRV
HHHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RMGHVMLGALPPGQWRYLGPDESF
HHHHEEEECCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]