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The map label for this gene is smpB [H]

Identifier: 186476076

GI number: 186476076

Start: 1476463

End: 1476909

Strand: Direct

Name: smpB [H]

Synonym: Bphy_1318

Alternate gene names: 186476076

Gene position: 1476463-1476909 (Clockwise)

Preceding gene: 186476073

Following gene: 186476080

Centisome position: 42.44

GC content: 59.51

Gene sequence:

>447_bases
ATGAGCATCATCGACAACAGGAAAGCGTTCTTCGACTACTCGGTCGAGGAGCGTTACGAGGCGGGGCTCGTGCTGGAGGG
CTGGGAAGTCAAGGCGCTGCGCGCCGGGCGCGGGCAGATCAAGGAAGGCTACGTCGTGATCAAGAACGCGGAGCTGTACC
TGATTGGCACGCACATTAGCCCGCTGCCCGAGGCATCGAAATTCGCCAATCTGGATCCGGTGCGCACGCGCAAGCTGCTT
TTGCACGCGGAGGAAATCAGCAAGCTGATCGGCAAGGTCGAGCAGCGCGGGTATACGCTCGTGCCGCTAAATTTCCATTA
CAAGAATGGGCGCGTGAAATGCGAGATCGGGCTTGCCAAGGGCAAAAAGCTGCACGACAAGCGTGAGACCGAAAAGAAGC
GGGATTGGGAGCGCGAAAAGGCGCGGTTGATGCGGACGCCGACGTAG

Upstream 100 bases:

>100_bases
GAAATAGCGAACGCGTGACAACTCAATGACGCCGCGGCGGGTTTTCGGCGGCCCGCTTTGCTAAAATCGCAATTTTAAAC
GAGTTGGCCGATTTCCATTC

Downstream 100 bases:

>100_bases
CCGTTTCTTGTCTGCGACGCAGTCGCAGACAAGAAACTTCAGCTCAAAGTCTTGCCACCCGACTTAACAATCGCCAGCGC
AGCGGAAATCATCGAACCCA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MSIIDNRKAFFDYSVEERYEAGLVLEGWEVKALRAGRGQIKEGYVVIKNAELYLIGTHISPLPEASKFANLDPVRTRKLL
LHAEEISKLIGKVEQRGYTLVPLNFHYKNGRVKCEIGLAKGKKLHDKRETEKKRDWEREKARLMRTPT

Sequences:

>Translated_148_residues
MSIIDNRKAFFDYSVEERYEAGLVLEGWEVKALRAGRGQIKEGYVVIKNAELYLIGTHISPLPEASKFANLDPVRTRKLL
LHAEEISKLIGKVEQRGYTLVPLNFHYKNGRVKCEIGLAKGKKLHDKRETEKKRDWEREKARLMRTPT
>Mature_147_residues
SIIDNRKAFFDYSVEERYEAGLVLEGWEVKALRAGRGQIKEGYVVIKNAELYLIGTHISPLPEASKFANLDPVRTRKLLL
HAEEISKLIGKVEQRGYTLVPLNFHYKNGRVKCEIGLAKGKKLHDKRETEKKRDWEREKARLMRTPT

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=146, Percent_Identity=47.2602739726027, Blast_Score=150, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17137; Mature: 17006

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIDNRKAFFDYSVEERYEAGLVLEGWEVKALRAGRGQIKEGYVVIKNAELYLIGTHIS
CCCCCCCCEEEECCHHHHCCCCEEEECCEEEEEECCCCCCCCCEEEEECCEEEEEECCCC
PLPEASKFANLDPVRTRKLLLHAEEISKLIGKVEQRGYTLVPLNFHYKNGRVKCEIGLAK
CCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEEECCC
GKKLHDKRETEKKRDWEREKARLMRTPT
CCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SIIDNRKAFFDYSVEERYEAGLVLEGWEVKALRAGRGQIKEGYVVIKNAELYLIGTHIS
CCCCCCCEEEECCHHHHCCCCEEEECCEEEEEECCCCCCCCCEEEEECCEEEEEECCCC
PLPEASKFANLDPVRTRKLLLHAEEISKLIGKVEQRGYTLVPLNFHYKNGRVKCEIGLAK
CCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEEECCC
GKKLHDKRETEKKRDWEREKARLMRTPT
CCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA