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The map label for this gene is ppsA [H]

Identifier: 186475864

GI number: 186475864

Start: 1241387

End: 1243831

Strand: Reverse

Name: ppsA [H]

Synonym: Bphy_1099

Alternate gene names: 186475864

Gene position: 1243831-1241387 (Counterclockwise)

Preceding gene: 186475865

Following gene: 186475863

Centisome position: 35.75

GC content: 63.52

Gene sequence:

>2445_bases
ATGAGCAGCAATGAAACGGAGGTCCTCTGGTTCGACGTGTTGCGCCGTACGGATGTGCCGTCCGTGGGCGGCAAGAATGC
GTCGCTCGGGGAGATGGTCGGTTGCCTCGCCAGCAAGGGCATCCGTGTTCCACCCGGCTTCGCGACGACAGCCGGCGCGT
ACTGGCAGTTCATCGACGCGAACGGGCTGCGCGAAAGCATGTCGACGGTGCTCGCGGAATTCCGCGCCAATCGCCGCTCG
TTGGCCGATACGGGTGAAACGGTTCGCCGGGCAATCCTTCGGGGCGAATGGCCCGCCAGCACGACGCGCGCCATTTGCGA
CGCGTATGCCGAACTCGGACGCCGCGTCGGCGTCGACGATCCGGATGTCGCCGTGCGTTCGAGCGCCACGGCTGAGGATT
TACCCGACGCGAGCTTCGCGGGACAGCAGGAGACCTTGCTCGCCATTCATGGCACGCATGCGTTGATGGATGCATGCCGC
CGGTGCTATGCCTCGCTATTCACGGATCGTGCCATCAGTTATCGCGAGGCACGAGGCTTCGATCACATGAAGGTTGCACT
GTCGGTCGGCGTGCAGCAGATGGTGCGCTCGGATCTGGGTGGCGCGGGCGTCGCGTTTTCGATCGATACCGAAACGGGCT
TCGACAAGATCGTATTGATCAGCGCGGCTTGGGGGCTCGGCGAGAACGTCGTGCAAGGCGCCGTCGATCCCGACGAATAT
GAAGTATTCAAACCGCTTTTGCAGAACGAAGCGTGGGTGCCGATTGTCGGCAAGAAGCGCGGCAGCAAGCTGAAGAAAAT
GGTGTACGCGAAAGGCGGCGCACATCCGACGAAGAACGTGCCGACTTCGAAAGCGGAGCGTGCGGCATTCGTCCTCGCCG
ACCGCGACGTGCTCACGATTTCACGCTGGGCGTGCGAAATCGAATCGCACTACGGGCAGCCGATGGACATCGAATGGGCA
AAGGACGGATTGACGGGAAACATCTTCATTGTCCAGGCGCGGCCCGAGACCGTCCAGTCGCGGCGTGAGGCGACCGCCGT
CAAGACCTTCCGGCTCATATCGGCGGGACGCGAGCTTGTCACGGGCGTGAGCGTCGGCCAGGCTATCGCGGCCGGGAACG
TGTGCGTCATCGACAGTCCTGCAGAGCGGGAGCGGTTCGTCGATGGCGCCGTGCTCGTGACGGCCGCGACGGATCCCGAC
TGGGTGCCCGTGATGCGGCGCGCTGCAGGTATCGTGACGGACCACGGGGGCCGCACGTCGCACGCGGCGATTGTGAGCCG
TGAACTGGGCCTTCCTGCCATCGTCGGCACGGGTCATGCCACGCGGGTGTTGCACGACGAGCAGGAAGTGACGGTGTCGT
GCGCGCAAGGCGAAGTCGGGCGCGTGTTCGAAGGCATGGCGGACTACCAGGTCGAGGAGATCGACTTCGCGGCGATCCCG
CAAACCCGAACCCAGGTCGTGCTGAATCTCGCGAACCCCGACGCCGCACTGCGCTGGTGGCGCATGCCGGCAGACGGCAT
CGGCCTGGCGAGAATGGAGTTCGTGATCAGCAATCACATCAAGATTCATCCGATGGCGCTCGCGTGCTTTGACCAGTTGA
AGGATGCCGAAGCGGCCCGGACAATCGCGGCGATGACGGAAGGGTACAGCGACAAGACGGAATACTTCGTCGACCGGCTT
GCCTGCGGACTCGGCCGCATAGCGGCCGTGTGTTATCCGCGGCCCGTGGTGGTGCGTTTGAGCGACTTCAAGACGAACGA
ATATGCGCACCTGACGGGAGGCAAGGCGTTCGAGCCGCTCGAGGAGAATCCGATGCTCGGCTTTCGCGGCGCATCGCGCT
ATTACTCGCCGCGCTACGGTCCGGGCTTTGCACTCGAATGCCGTGCACTGCGCCGCCTGCGAATGGATATGGGCCTGAAC
AACGTGATCGTCATGGTGCCGTTTTGCCGGACGCCGGACGAAGCGGATCGCGTCCTCGACGCGATGGCGGCCAATGGCCT
CAGGCGCGCAGAGGACGGATTGCAGGTCTACGTGATGTGCGAGATTCCGTCGAACGTCATCCTCGCCGACGCGTTCGCGC
AGCGGTTCGATGGGTTCTCGATCGGCAGCAACGATCTGACGCAACTCACGCTGGGCGTCGACCGGGACTCCGCAGAACTG
GCATCGCTGTTCGACGAACGCAACGAAGCCGTGCGCTGGATGATCCGCGAAGTCATCGAACGGGCGCATCGAGCCGGTGC
GAAAGTCAGCCTATGTGGCGAAGCGCCGAGTGTGCATCCCGAGTATGCAAGCTTTCTGGTGGAATGCGGCATCGACTCCA
TCTCGGTGAGTCCCGACAGTTTCATCGCAGTCAAGCAGCACGTCGCGCAAGCGGAACGGGAAGCGGCGCATCGTGCGACG
GGCGAGCAGACGCGCGGATCGATACTGTCTCAAACGGTGCGGTAG

Upstream 100 bases:

>100_bases
TTTCCGCGCTGGCCAGATGAAGCTTCCGTGGATGTTCGCGGGATACGTCAGCATCTGAGAGAGACGGGAGCACGGGTGAC
TCACCAGCGAGGAGCAGCCC

Downstream 100 bases:

>100_bases
GGCCTTGTCATGATCGACGTTCTCACAGTCACCCTGAATCCCGCGGTCGATCTATCGACCTCTGTTGAGCGCATCGTGGA
TACGCACAAGCTGCGATGCG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 814; Mature: 813

Protein sequence:

>814_residues
MSSNETEVLWFDVLRRTDVPSVGGKNASLGEMVGCLASKGIRVPPGFATTAGAYWQFIDANGLRESMSTVLAEFRANRRS
LADTGETVRRAILRGEWPASTTRAICDAYAELGRRVGVDDPDVAVRSSATAEDLPDASFAGQQETLLAIHGTHALMDACR
RCYASLFTDRAISYREARGFDHMKVALSVGVQQMVRSDLGGAGVAFSIDTETGFDKIVLISAAWGLGENVVQGAVDPDEY
EVFKPLLQNEAWVPIVGKKRGSKLKKMVYAKGGAHPTKNVPTSKAERAAFVLADRDVLTISRWACEIESHYGQPMDIEWA
KDGLTGNIFIVQARPETVQSRREATAVKTFRLISAGRELVTGVSVGQAIAAGNVCVIDSPAERERFVDGAVLVTAATDPD
WVPVMRRAAGIVTDHGGRTSHAAIVSRELGLPAIVGTGHATRVLHDEQEVTVSCAQGEVGRVFEGMADYQVEEIDFAAIP
QTRTQVVLNLANPDAALRWWRMPADGIGLARMEFVISNHIKIHPMALACFDQLKDAEAARTIAAMTEGYSDKTEYFVDRL
ACGLGRIAAVCYPRPVVVRLSDFKTNEYAHLTGGKAFEPLEENPMLGFRGASRYYSPRYGPGFALECRALRRLRMDMGLN
NVIVMVPFCRTPDEADRVLDAMAANGLRRAEDGLQVYVMCEIPSNVILADAFAQRFDGFSIGSNDLTQLTLGVDRDSAEL
ASLFDERNEAVRWMIREVIERAHRAGAKVSLCGEAPSVHPEYASFLVECGIDSISVSPDSFIAVKQHVAQAEREAAHRAT
GEQTRGSILSQTVR

Sequences:

>Translated_814_residues
MSSNETEVLWFDVLRRTDVPSVGGKNASLGEMVGCLASKGIRVPPGFATTAGAYWQFIDANGLRESMSTVLAEFRANRRS
LADTGETVRRAILRGEWPASTTRAICDAYAELGRRVGVDDPDVAVRSSATAEDLPDASFAGQQETLLAIHGTHALMDACR
RCYASLFTDRAISYREARGFDHMKVALSVGVQQMVRSDLGGAGVAFSIDTETGFDKIVLISAAWGLGENVVQGAVDPDEY
EVFKPLLQNEAWVPIVGKKRGSKLKKMVYAKGGAHPTKNVPTSKAERAAFVLADRDVLTISRWACEIESHYGQPMDIEWA
KDGLTGNIFIVQARPETVQSRREATAVKTFRLISAGRELVTGVSVGQAIAAGNVCVIDSPAERERFVDGAVLVTAATDPD
WVPVMRRAAGIVTDHGGRTSHAAIVSRELGLPAIVGTGHATRVLHDEQEVTVSCAQGEVGRVFEGMADYQVEEIDFAAIP
QTRTQVVLNLANPDAALRWWRMPADGIGLARMEFVISNHIKIHPMALACFDQLKDAEAARTIAAMTEGYSDKTEYFVDRL
ACGLGRIAAVCYPRPVVVRLSDFKTNEYAHLTGGKAFEPLEENPMLGFRGASRYYSPRYGPGFALECRALRRLRMDMGLN
NVIVMVPFCRTPDEADRVLDAMAANGLRRAEDGLQVYVMCEIPSNVILADAFAQRFDGFSIGSNDLTQLTLGVDRDSAEL
ASLFDERNEAVRWMIREVIERAHRAGAKVSLCGEAPSVHPEYASFLVECGIDSISVSPDSFIAVKQHVAQAEREAAHRAT
GEQTRGSILSQTVR
>Mature_813_residues
SSNETEVLWFDVLRRTDVPSVGGKNASLGEMVGCLASKGIRVPPGFATTAGAYWQFIDANGLRESMSTVLAEFRANRRSL
ADTGETVRRAILRGEWPASTTRAICDAYAELGRRVGVDDPDVAVRSSATAEDLPDASFAGQQETLLAIHGTHALMDACRR
CYASLFTDRAISYREARGFDHMKVALSVGVQQMVRSDLGGAGVAFSIDTETGFDKIVLISAAWGLGENVVQGAVDPDEYE
VFKPLLQNEAWVPIVGKKRGSKLKKMVYAKGGAHPTKNVPTSKAERAAFVLADRDVLTISRWACEIESHYGQPMDIEWAK
DGLTGNIFIVQARPETVQSRREATAVKTFRLISAGRELVTGVSVGQAIAAGNVCVIDSPAERERFVDGAVLVTAATDPDW
VPVMRRAAGIVTDHGGRTSHAAIVSRELGLPAIVGTGHATRVLHDEQEVTVSCAQGEVGRVFEGMADYQVEEIDFAAIPQ
TRTQVVLNLANPDAALRWWRMPADGIGLARMEFVISNHIKIHPMALACFDQLKDAEAARTIAAMTEGYSDKTEYFVDRLA
CGLGRIAAVCYPRPVVVRLSDFKTNEYAHLTGGKAFEPLEENPMLGFRGASRYYSPRYGPGFALECRALRRLRMDMGLNN
VIVMVPFCRTPDEADRVLDAMAANGLRRAEDGLQVYVMCEIPSNVILADAFAQRFDGFSIGSNDLTQLTLGVDRDSAELA
SLFDERNEAVRWMIREVIERAHRAGAKVSLCGEAPSVHPEYASFLVECGIDSISVSPDSFIAVKQHVAQAEREAAHRATG
EQTRGSILSQTVR

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=803, Percent_Identity=48.692403486924, Blast_Score=754, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=436, Percent_Identity=26.1467889908257, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI1788726, Length=232, Percent_Identity=29.7413793103448, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI48994992, Length=407, Percent_Identity=26.7813267813268, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1789193, Length=420, Percent_Identity=26.1904761904762, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17564524, Length=226, Percent_Identity=27.8761061946903, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 88637; Mature: 88505

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNETEVLWFDVLRRTDVPSVGGKNASLGEMVGCLASKGIRVPPGFATTAGAYWQFIDA
CCCCCCEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECC
NGLRESMSTVLAEFRANRRSLADTGETVRRAILRGEWPASTTRAICDAYAELGRRVGVDD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
PDVAVRSSATAEDLPDASFAGQQETLLAIHGTHALMDACRRCYASLFTDRAISYREARGF
CCCCEECCCCHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DHMKVALSVGVQQMVRSDLGGAGVAFSIDTETGFDKIVLISAAWGLGENVVQGAVDPDEY
CHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHCCCCCHHH
EVFKPLLQNEAWVPIVGKKRGSKLKKMVYAKGGAHPTKNVPTSKAERAAFVLADRDVLTI
HHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHCEEEEEECCCEEEH
SRWACEIESHYGQPMDIEWAKDGLTGNIFIVQARPETVQSRREATAVKTFRLISAGRELV
HHHHHHHHHHCCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCHHHH
TGVSVGQAIAAGNVCVIDSPAERERFVDGAVLVTAATDPDWVPVMRRAAGIVTDHGGRTS
HHHHHHHHHHCCCEEEECCCHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCEECCCCCCC
HAAIVSRELGLPAIVGTGHATRVLHDEQEVTVSCAQGEVGRVFEGMADYQVEEIDFAAIP
HHHHHHHHCCCCEEEECCCCEEEEECCCCEEEEECCCCHHHHHHHHHCCEEHHCCEEECC
QTRTQVVLNLANPDAALRWWRMPADGIGLARMEFVISNHIKIHPMALACFDQLKDAEAAR
CCCEEEEEEECCCCHHHEEECCCCCCCCHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHH
TIAAMTEGYSDKTEYFVDRLACGLGRIAAVCYPRPVVVRLSDFKTNEYAHLTGGKAFEPL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCCCC
EENPMLGFRGASRYYSPRYGPGFALECRALRRLRMDMGLNNVIVMVPFCRTPDEADRVLD
CCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
AMAANGLRRAEDGLQVYVMCEIPSNVILADAFAQRFDGFSIGSNDLTQLTLGVDRDSAEL
HHHHCCCHHCCCCCEEEEEEECCCCEEEHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHH
ASLFDERNEAVRWMIREVIERAHRAGAKVSLCGEAPSVHPEYASFLVECGIDSISVSPDS
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCCCEECCCCH
FIAVKQHVAQAEREAAHRATGEQTRGSILSQTVR
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SSNETEVLWFDVLRRTDVPSVGGKNASLGEMVGCLASKGIRVPPGFATTAGAYWQFIDA
CCCCCEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECC
NGLRESMSTVLAEFRANRRSLADTGETVRRAILRGEWPASTTRAICDAYAELGRRVGVDD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
PDVAVRSSATAEDLPDASFAGQQETLLAIHGTHALMDACRRCYASLFTDRAISYREARGF
CCCCEECCCCHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DHMKVALSVGVQQMVRSDLGGAGVAFSIDTETGFDKIVLISAAWGLGENVVQGAVDPDEY
CHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHCCCCCHHH
EVFKPLLQNEAWVPIVGKKRGSKLKKMVYAKGGAHPTKNVPTSKAERAAFVLADRDVLTI
HHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHCEEEEEECCCEEEH
SRWACEIESHYGQPMDIEWAKDGLTGNIFIVQARPETVQSRREATAVKTFRLISAGRELV
HHHHHHHHHHCCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCHHHH
TGVSVGQAIAAGNVCVIDSPAERERFVDGAVLVTAATDPDWVPVMRRAAGIVTDHGGRTS
HHHHHHHHHHCCCEEEECCCHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCEECCCCCCC
HAAIVSRELGLPAIVGTGHATRVLHDEQEVTVSCAQGEVGRVFEGMADYQVEEIDFAAIP
HHHHHHHHCCCCEEEECCCCEEEEECCCCEEEEECCCCHHHHHHHHHCCEEHHCCEEECC
QTRTQVVLNLANPDAALRWWRMPADGIGLARMEFVISNHIKIHPMALACFDQLKDAEAAR
CCCEEEEEEECCCCHHHEEECCCCCCCCHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHH
TIAAMTEGYSDKTEYFVDRLACGLGRIAAVCYPRPVVVRLSDFKTNEYAHLTGGKAFEPL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCCCC
EENPMLGFRGASRYYSPRYGPGFALECRALRRLRMDMGLNNVIVMVPFCRTPDEADRVLD
CCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
AMAANGLRRAEDGLQVYVMCEIPSNVILADAFAQRFDGFSIGSNDLTQLTLGVDRDSAEL
HHHHCCCHHCCCCCEEEEEEECCCCEEEHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHH
ASLFDERNEAVRWMIREVIERAHRAGAKVSLCGEAPSVHPEYASFLVECGIDSISVSPDS
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCCCEECCCCH
FIAVKQHVAQAEREAAHRATGEQTRGSILSQTVR
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]