| Definition | Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010622 |
| Length | 3,479,187 |
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The map label for this gene is epsB [H]
Identifier: 186475826
GI number: 186475826
Start: 1191585
End: 1193777
Strand: Direct
Name: epsB [H]
Synonym: Bphy_1061
Alternate gene names: 186475826
Gene position: 1191585-1193777 (Clockwise)
Preceding gene: 186475825
Following gene: 186475827
Centisome position: 34.25
GC content: 65.12
Gene sequence:
>2193_bases ATGCAGGCCAGAACCGAAGAAGAGGACGTGGTCCTCGGCCAACTGCTGCAGGTGATCATCGACGACATCGGGTGGCTGAT CGGCATCGCGGCGACCATCGTCGCGATGGCCGGCTTCTACTGCTATGTCGCGAAGCCCGTGTATTCGGCCGATGCGCACG TGCGCGTGGAGGCGTCGGACAACACATCGCAGGCGCTCACGCAGACGCAGACGGGCGCAACGATCTCGACGGGGCAGAAC ACGATCCCCACCGACGCCGAGATCGAAATGATCAAGAGCCGGGGCGTCGTCGCGCCCGTGGTGCAGCAAATGAAGCTCAA CTTCTCGGTGACGCCGAAGACGCTGCCCATTCTCGGCAGTCTCGCCGCGCGTGTCGCGACGCCAGGTCAGCCCGCCAAGC CGTGGCTCGGCCTCGGCAGCTATGCCTGGGGCGGCGAAGTGGCTGCTGTCGATTCGATTGACGTGACGCCCGCGCTCGAA GGCCAGAAGCTGATCCTCACCGCAATGGACAACGGCCGCTATACGCTGATGACGCCCGACGGCCAGCTGCTGTTGCGCGG CCAGGCAGGCGAGCCCGCGCAGGGCGGCGGCGTCACGCTGTTCGTGAAGCAGCTGGTGGCCCGTCCGGGCACACAGTTCA CTGTGATGCGCGCGAACGATCTGGACGCGATCGCCGCGTTCCAGTCCGCGATCAACGTGCAGGAGCAGGGCAAGCAGACG GGCGTGATCTCGATCTCACTAGAAGACCAAAGCCCCGAGCACGCCGCCGCCGTCGCCAATGCACTTGCGCAGTCGTATCT GCTCGAGCACATTCGCACCAAGCAGGCCGACGCGAGCCGCATGCTCGACTTCCTGAAGAGCGAAGAGCCGCGCCTCAAGT CCGATCTCGAACACGCGGAAGCCGCGTTGACGGCGTATCAGAGCAAGAGCGGCTCGATCAACGCGAGCGACGAGGCGAAG GTCTATCTGGAAGGCAGCGTGCAGTACGAGCAGCAGATTTCGGCCTTGCGTCTGCAGATTTCCCAGCTCGAACAGCGCTA TGGCGACGATCACCCGATGATCAAGGCCGCGCAGCAGCAGATGGCCGAGCTGCAGGCGCAGCGCACCAAATACGCGGACC GTTTCCGCGATCTGCCCGCGACGGAAGTGAAGGCCGTGCAGTTGCAGCGCGACGCGAAGGTCGCGGAAGACATCTACGTG CTGCTGCTCAACCGCGTGCAGGAGCTGTCGGTGCAAAAGGCGGGCACGGGCGGCAACGTGCATATCGTCGACGCGGCGCT GCGTCCCGGTTCGCCCATCAAGCCGAAGAAGGTGCTGATCTTGTCGGCGGCGACGATTCTCGGGTTGATCGTCGGCACCG GCTTCGTGTTCCTGCGCCGCAATATGTTCAAGGGCATCGACGACCCGGAGACGGTCGAGCGCGCGTTTCATCTGCCCGTG TACGGTCTGGTGCCGCTGTCGGCTGAACAGGCGCTGCTCGAAAACGCCGCGGTGCGCGGCGGCGCGCGCGAGCGTGCGGT GCTCGCGAACGCCAAGCCGAAGGACGTGTGCATCGAAAGCCTGCGCAGCCTGCGCACGTCGATGCAGTTCACGCTGCAGG ACGCGCGCAATCGCATCGTGATGTTCACGGGCCCGCTGCCGGGCGTCGGCAAGAGCTTCCTGACGATCAACCTGGGCGTG CTGCTCGCGCATTCGGGCAAGCGGGTGCTGATGATCGACGGCGACATGCGGCGCGGCGCGCTTGAAAAGCATGTGGGCGG CTCGCCGGACAACGGTCTGTCGGAACTGCTGAGCGGACAGATTTCGCTGGAAGAAGCAGTGCGCGCGACGGAGATCGACA ACCTCTCGTTCATCTCGTGCGGCCGCCGTCCGCCGAATCCGTCCGAGCTGCTGATGTCGCCTCGCTTGCCTCAGTACCTC GATGGACTGGCCAAGCGTTACGACGTGATCCTGATCGACACGCCGCCCGTGCTGGCCGTGACCGACGCGTCGATCATCGG CGGCTATGCGGGTTCGACCTTCTTCGTCGTGCGCTCGGGCATGCACAGCGAAGGCGAGATCGCCGATGCGTTGAAGCGCC TGCATTCAGCGGGCGTGCACGTGCAGGGCGCGATCTTCAACGCGATGCCGCCGCGCGCGCGCGGCAACTACGACCGCAGC TATGCGGCCGTACAGGAATACCTGAGCACCTGA
Upstream 100 bases:
>100_bases GGATGCAACGCGCCGGATGCGGGCGGCGACCCGACCCGGGTCAACCCTGAGCACTCAAGCGAGTATCCAAGTGAGCACTC AAGCGAATCCACAACTGGCC
Downstream 100 bases:
>100_bases TGCGCAGCGCGCCTTGCCGGTGCGGCGGGGCGCATGCGCCGTCGACAAGCCACGGAGAGTACAGGATGAAGATAACGGTA CTGGTGCCGACATACCGCCG
Product: exopolysaccharide tyrosine-protein kinase
Products: ADP; protein tyrosine phosphate [C]
Alternate protein names: EPS I polysaccharide export protein epsB [H]
Number of amino acids: Translated: 730; Mature: 730
Protein sequence:
>730_residues MQARTEEEDVVLGQLLQVIIDDIGWLIGIAATIVAMAGFYCYVAKPVYSADAHVRVEASDNTSQALTQTQTGATISTGQN TIPTDAEIEMIKSRGVVAPVVQQMKLNFSVTPKTLPILGSLAARVATPGQPAKPWLGLGSYAWGGEVAAVDSIDVTPALE GQKLILTAMDNGRYTLMTPDGQLLLRGQAGEPAQGGGVTLFVKQLVARPGTQFTVMRANDLDAIAAFQSAINVQEQGKQT GVISISLEDQSPEHAAAVANALAQSYLLEHIRTKQADASRMLDFLKSEEPRLKSDLEHAEAALTAYQSKSGSINASDEAK VYLEGSVQYEQQISALRLQISQLEQRYGDDHPMIKAAQQQMAELQAQRTKYADRFRDLPATEVKAVQLQRDAKVAEDIYV LLLNRVQELSVQKAGTGGNVHIVDAALRPGSPIKPKKVLILSAATILGLIVGTGFVFLRRNMFKGIDDPETVERAFHLPV YGLVPLSAEQALLENAAVRGGARERAVLANAKPKDVCIESLRSLRTSMQFTLQDARNRIVMFTGPLPGVGKSFLTINLGV LLAHSGKRVLMIDGDMRRGALEKHVGGSPDNGLSELLSGQISLEEAVRATEIDNLSFISCGRRPPNPSELLMSPRLPQYL DGLAKRYDVILIDTPPVLAVTDASIIGGYAGSTFFVVRSGMHSEGEIADALKRLHSAGVHVQGAIFNAMPPRARGNYDRS YAAVQEYLST
Sequences:
>Translated_730_residues MQARTEEEDVVLGQLLQVIIDDIGWLIGIAATIVAMAGFYCYVAKPVYSADAHVRVEASDNTSQALTQTQTGATISTGQN TIPTDAEIEMIKSRGVVAPVVQQMKLNFSVTPKTLPILGSLAARVATPGQPAKPWLGLGSYAWGGEVAAVDSIDVTPALE GQKLILTAMDNGRYTLMTPDGQLLLRGQAGEPAQGGGVTLFVKQLVARPGTQFTVMRANDLDAIAAFQSAINVQEQGKQT GVISISLEDQSPEHAAAVANALAQSYLLEHIRTKQADASRMLDFLKSEEPRLKSDLEHAEAALTAYQSKSGSINASDEAK VYLEGSVQYEQQISALRLQISQLEQRYGDDHPMIKAAQQQMAELQAQRTKYADRFRDLPATEVKAVQLQRDAKVAEDIYV LLLNRVQELSVQKAGTGGNVHIVDAALRPGSPIKPKKVLILSAATILGLIVGTGFVFLRRNMFKGIDDPETVERAFHLPV YGLVPLSAEQALLENAAVRGGARERAVLANAKPKDVCIESLRSLRTSMQFTLQDARNRIVMFTGPLPGVGKSFLTINLGV LLAHSGKRVLMIDGDMRRGALEKHVGGSPDNGLSELLSGQISLEEAVRATEIDNLSFISCGRRPPNPSELLMSPRLPQYL DGLAKRYDVILIDTPPVLAVTDASIIGGYAGSTFFVVRSGMHSEGEIADALKRLHSAGVHVQGAIFNAMPPRARGNYDRS YAAVQEYLST >Mature_730_residues MQARTEEEDVVLGQLLQVIIDDIGWLIGIAATIVAMAGFYCYVAKPVYSADAHVRVEASDNTSQALTQTQTGATISTGQN TIPTDAEIEMIKSRGVVAPVVQQMKLNFSVTPKTLPILGSLAARVATPGQPAKPWLGLGSYAWGGEVAAVDSIDVTPALE GQKLILTAMDNGRYTLMTPDGQLLLRGQAGEPAQGGGVTLFVKQLVARPGTQFTVMRANDLDAIAAFQSAINVQEQGKQT GVISISLEDQSPEHAAAVANALAQSYLLEHIRTKQADASRMLDFLKSEEPRLKSDLEHAEAALTAYQSKSGSINASDEAK VYLEGSVQYEQQISALRLQISQLEQRYGDDHPMIKAAQQQMAELQAQRTKYADRFRDLPATEVKAVQLQRDAKVAEDIYV LLLNRVQELSVQKAGTGGNVHIVDAALRPGSPIKPKKVLILSAATILGLIVGTGFVFLRRNMFKGIDDPETVERAFHLPV YGLVPLSAEQALLENAAVRGGARERAVLANAKPKDVCIESLRSLRTSMQFTLQDARNRIVMFTGPLPGVGKSFLTINLGV LLAHSGKRVLMIDGDMRRGALEKHVGGSPDNGLSELLSGQISLEEAVRATEIDNLSFISCGRRPPNPSELLMSPRLPQYL DGLAKRYDVILIDTPPVLAVTDASIIGGYAGSTFFVVRSGMHSEGEIADALKRLHSAGVHVQGAIFNAMPPRARGNYDRS YAAVQEYLST
Specific function: Probably involved in polymerization and/or export of exopolysaccharide EPS I which functions as a virulence factor. May be involved in an ATP-dependent process in the pathway for EPS I production, possibly export of the trimeric repeat units across the in
COG id: COG3206
COG function: function code M; Uncharacterized protein involved in exopolysaccharide biosynthesis
Gene ontology:
Cell location: Cell inner membrane; Multi-pass membrane protein [H]
Metaboloic importance: Non Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the etk/wzc family [H]
Homologues:
Organism=Escherichia coli, GI87082032, Length=721, Percent_Identity=34.8127600554785, Blast_Score=427, Evalue=1e-121, Organism=Escherichia coli, GI1787216, Length=727, Percent_Identity=31.9119669876204, Blast_Score=397, Evalue=1e-111,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002586 - InterPro: IPR005702 - InterPro: IPR005700 - InterPro: IPR003856 [H]
Pfam domain/function: PF01656 CbiA; PF02706 Wzz [H]
EC number: 2.7.1.112 [C]
Molecular weight: Translated: 78693; Mature: 78693
Theoretical pI: Translated: 6.40; Mature: 6.40
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQARTEEEDVVLGQLLQVIIDDIGWLIGIAATIVAMAGFYCYVAKPVYSADAHVRVEASD CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEECCCCEEEEEECC NTSQALTQTQTGATISTGQNTIPTDAEIEMIKSRGVVAPVVQQMKLNFSVTPKTLPILGS CCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCEEECCCHHHHHHH LAARVATPGQPAKPWLGLGSYAWGGEVAAVDSIDVTPALEGQKLILTAMDNGRYTLMTPD HHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCEEEEECCC GQLLLRGQAGEPAQGGGVTLFVKQLVARPGTQFTVMRANDLDAIAAFQSAINVQEQGKQT CCEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCHHHCCCCC GVISISLEDQSPEHAAAVANALAQSYLLEHIRTKQADASRMLDFLKSEEPRLKSDLEHAE CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHH AALTAYQSKSGSINASDEAKVYLEGSVQYEQQISALRLQISQLEQRYGDDHPMIKAAQQQ HHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH MAELQAQRTKYADRFRDLPATEVKAVQLQRDAKVAEDIYVLLLNRVQELSVQKAGTGGNV HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE HIVDAALRPGSPIKPKKVLILSAATILGLIVGTGFVFLRRNMFKGIDDPETVERAFHLPV EEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCE YGLVPLSAEQALLENAAVRGGARERAVLANAKPKDVCIESLRSLRTSMQFTLQDARNRIV EEEECCCHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE MFTGPLPGVGKSFLTINLGVLLAHSGKRVLMIDGDMRRGALEKHVGGSPDNGLSELLSGQ EEECCCCCCCCCEEEEEEEEEEECCCCEEEEECCCHHHCHHHHHCCCCCCCCHHHHHCCC ISLEEAVRATEIDNLSFISCGRRPPNPSELLMSPRLPQYLDGLAKRYDVILIDTPPVLAV CCHHHHHHHHCCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHCCEEEECCCCEEEE TDASIIGGYAGSTFFVVRSGMHSEGEIADALKRLHSAGVHVQGAIFNAMPPRARGNYDRS ECCHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHH YAAVQEYLST HHHHHHHHCC >Mature Secondary Structure MQARTEEEDVVLGQLLQVIIDDIGWLIGIAATIVAMAGFYCYVAKPVYSADAHVRVEASD CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEECCCCEEEEEECC NTSQALTQTQTGATISTGQNTIPTDAEIEMIKSRGVVAPVVQQMKLNFSVTPKTLPILGS CCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCEEECCCHHHHHHH LAARVATPGQPAKPWLGLGSYAWGGEVAAVDSIDVTPALEGQKLILTAMDNGRYTLMTPD HHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCEEEEECCC GQLLLRGQAGEPAQGGGVTLFVKQLVARPGTQFTVMRANDLDAIAAFQSAINVQEQGKQT CCEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCHHHCCCCC GVISISLEDQSPEHAAAVANALAQSYLLEHIRTKQADASRMLDFLKSEEPRLKSDLEHAE CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHH AALTAYQSKSGSINASDEAKVYLEGSVQYEQQISALRLQISQLEQRYGDDHPMIKAAQQQ HHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH MAELQAQRTKYADRFRDLPATEVKAVQLQRDAKVAEDIYVLLLNRVQELSVQKAGTGGNV HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE HIVDAALRPGSPIKPKKVLILSAATILGLIVGTGFVFLRRNMFKGIDDPETVERAFHLPV EEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCE YGLVPLSAEQALLENAAVRGGARERAVLANAKPKDVCIESLRSLRTSMQFTLQDARNRIV EEEECCCHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE MFTGPLPGVGKSFLTINLGVLLAHSGKRVLMIDGDMRRGALEKHVGGSPDNGLSELLSGQ EEECCCCCCCCCEEEEEEEEEEECCCCEEEEECCCHHHCHHHHHCCCCCCCCHHHHHCCC ISLEEAVRATEIDNLSFISCGRRPPNPSELLMSPRLPQYLDGLAKRYDVILIDTPPVLAV CCHHHHHHHHCCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHCCEEEECCCCEEEE TDASIIGGYAGSTFFVVRSGMHSEGEIADALKRLHSAGVHVQGAIFNAMPPRARGNYDRS ECCHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHH YAAVQEYLST HHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: ATP; a protein tyrosine [C]
Specific reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 11823852 [H]