Click here to switch to the map view.

The map label for this gene is otsA [H]

Identifier: 186475813

GI number: 186475813

Start: 1176723

End: 1178162

Strand: Direct

Name: otsA [H]

Synonym: Bphy_1048

Alternate gene names: 186475813

Gene position: 1176723-1178162 (Clockwise)

Preceding gene: 186475812

Following gene: 186475814

Centisome position: 33.82

GC content: 62.08

Gene sequence:

>1440_bases
ATGGGCCGACTGATCGTCGTGTCGAATCGCGTGGCAACGCCGACTGAAACGAGAGGCTCGGCGGGCGGGCTGGCCGTCGG
CGTGTTCGGCGCGCTGAAGGATGCGGGCGGGGTGTGGTTCGGGTGGAGCGGCGACGTCGTCAGCGAGACGGTGGCGAACG
CCGGGCCCACGCTCGAAACGGACGGCAGCGTCACGTTTGCCACCACAGGCCTCACGCGCAAGGACTACGACCAGTACTAC
CGGGGCTTTTCGAACGCGACGCTGTGGCCCGTCTTCCACTATCGCAACGATCTCGCTCGCTACGAACGCGAGGAATACGC
AGGCTACCGGCGGGTCAATGCGTGGCTCTCGCACAAGCTCATCAAGCTGCTGGAGCCTGACGACATTATCTGGGTCCACG
ACTATCACCTCATCCCCTTCGCGGAAGCGCTGCGATCCGAGGGCGTCAGGAATCGCATCGGTTTTTTCCTGCACATTCCG
TTTCCGGCGCCGCAGATCCTGTTGAACATTCCGCCGCATGAAGAACTGGTGAAGTCGTTATGTTGCTACGACCTGCTCGG
TTTTCAGACCGAAAACGACGAGCTCGCGTTTCATGACTATCTGCGCCGGCACGCACGCGGCACCGTCTCGGCTGACGGAC
ACGCGCAAGCGTTCGGTCGGCAGATGCGCACGGGCGTCTACCGGATCGGCGTGTTCCCCGACGAGATTGCCGAGCAGGCA
AAGCGCTATGAGAGCCGTCAGCACGTGCTCGACCTCAAGCAGAGCCTGGAAGGGCGCAAGCTGATCATGAGCGTCGACCG
TCTCGACTATTCGAAGGGGCTGGTCGAGCGCTTCCGCGCATTCGAGGCGCTGCTGGAGCGCTCGCCCGAATGGCGCGGCA
ATGTCACACTTGTGCAGATTGCGCCGCCCACGCGCTCCGACGTCACGACGTACCAGGACATCCGTCAGCAACTCGAATAC
GAAGCCGGGCGTATCAACGGAAAATACTCAGGCCTGGACTACACGCCCATTCGTTATTTGAATCAGCAATACGACCGCTG
GAAGCTGATGTCGCTGTTCCGCGAGTCGCAGGTCGGATTCGTCACGCCCTTGCACGACGGGATGAATCTCGTCGCGAAGG
AATATGTCGCCGCCCAGAACCCGGACGATCCAGGTGTGCTCGTACTGTCGATGTTCGCGGGCGCGGCCGCCGAACTGGAA
GGGGCATTGATCGTGAATCCTCACGATTCGATCGGCATGTGCGAAGCGTTGCAGCGTGCGCTCGCGATGCCGCTCGACGA
ACGCAAGCGGCGTCACGATATCGACATGCAGGCGCTGCGCAAGAACGATCTCGGTGTGTGGCGCGACTCGTTCCTGCGCG
ATCTGCGCAGCGTGCCGCTTGCCGGGTCGCCGAGCGACGCTTCCGGAACCTCGACAGACTCAGCAAAAAGGACAAAGTGA

Upstream 100 bases:

>100_bases
TGTGCAACTGATCGGGTGCAACCGCAGTCGAACCAGCACTGAATAATGCGCGCCAGCCGCCGTGGACTGGCCGCGCCAAC
CAGAGCGGAGGGACAGCAAC

Downstream 100 bases:

>100_bases
TGGGCGTTATACAGCATCGACATTCGTGCGCGATCTGCTGGTGCCGCTCGCGCGCCAGAACGTGATCACGGGCGCGAATG
GAAGCGGCAAATCGTGCGTG

Product: alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MGRLIVVSNRVATPTETRGSAGGLAVGVFGALKDAGGVWFGWSGDVVSETVANAGPTLETDGSVTFATTGLTRKDYDQYY
RGFSNATLWPVFHYRNDLARYEREEYAGYRRVNAWLSHKLIKLLEPDDIIWVHDYHLIPFAEALRSEGVRNRIGFFLHIP
FPAPQILLNIPPHEELVKSLCCYDLLGFQTENDELAFHDYLRRHARGTVSADGHAQAFGRQMRTGVYRIGVFPDEIAEQA
KRYESRQHVLDLKQSLEGRKLIMSVDRLDYSKGLVERFRAFEALLERSPEWRGNVTLVQIAPPTRSDVTTYQDIRQQLEY
EAGRINGKYSGLDYTPIRYLNQQYDRWKLMSLFRESQVGFVTPLHDGMNLVAKEYVAAQNPDDPGVLVLSMFAGAAAELE
GALIVNPHDSIGMCEALQRALAMPLDERKRRHDIDMQALRKNDLGVWRDSFLRDLRSVPLAGSPSDASGTSTDSAKRTK

Sequences:

>Translated_479_residues
MGRLIVVSNRVATPTETRGSAGGLAVGVFGALKDAGGVWFGWSGDVVSETVANAGPTLETDGSVTFATTGLTRKDYDQYY
RGFSNATLWPVFHYRNDLARYEREEYAGYRRVNAWLSHKLIKLLEPDDIIWVHDYHLIPFAEALRSEGVRNRIGFFLHIP
FPAPQILLNIPPHEELVKSLCCYDLLGFQTENDELAFHDYLRRHARGTVSADGHAQAFGRQMRTGVYRIGVFPDEIAEQA
KRYESRQHVLDLKQSLEGRKLIMSVDRLDYSKGLVERFRAFEALLERSPEWRGNVTLVQIAPPTRSDVTTYQDIRQQLEY
EAGRINGKYSGLDYTPIRYLNQQYDRWKLMSLFRESQVGFVTPLHDGMNLVAKEYVAAQNPDDPGVLVLSMFAGAAAELE
GALIVNPHDSIGMCEALQRALAMPLDERKRRHDIDMQALRKNDLGVWRDSFLRDLRSVPLAGSPSDASGTSTDSAKRTK
>Mature_478_residues
GRLIVVSNRVATPTETRGSAGGLAVGVFGALKDAGGVWFGWSGDVVSETVANAGPTLETDGSVTFATTGLTRKDYDQYYR
GFSNATLWPVFHYRNDLARYEREEYAGYRRVNAWLSHKLIKLLEPDDIIWVHDYHLIPFAEALRSEGVRNRIGFFLHIPF
PAPQILLNIPPHEELVKSLCCYDLLGFQTENDELAFHDYLRRHARGTVSADGHAQAFGRQMRTGVYRIGVFPDEIAEQAK
RYESRQHVLDLKQSLEGRKLIMSVDRLDYSKGLVERFRAFEALLERSPEWRGNVTLVQIAPPTRSDVTTYQDIRQQLEYE
AGRINGKYSGLDYTPIRYLNQQYDRWKLMSLFRESQVGFVTPLHDGMNLVAKEYVAAQNPDDPGVLVLSMFAGAAAELEG
ALIVNPHDSIGMCEALQRALAMPLDERKRRHDIDMQALRKNDLGVWRDSFLRDLRSVPLAGSPSDASGTSTDSAKRTK

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=459, Percent_Identity=55.9912854030501, Blast_Score=490, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI115533558, Length=358, Percent_Identity=30.7262569832402, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI115533560, Length=358, Percent_Identity=30.7262569832402, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71987012, Length=418, Percent_Identity=29.4258373205742, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319602, Length=470, Percent_Identity=33.4042553191489, Blast_Score=272, Evalue=9e-74,
Organism=Saccharomyces cerevisiae, GI6323537, Length=419, Percent_Identity=26.9689737470167, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6320279, Length=383, Percent_Identity=28.1984334203655, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323917, Length=441, Percent_Identity=25.8503401360544, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19920676, Length=486, Percent_Identity=34.7736625514403, Blast_Score=266, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 54074; Mature: 53943

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLIVVSNRVATPTETRGSAGGLAVGVFGALKDAGGVWFGWSGDVVSETVANAGPTLET
CCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCCCCC
DGSVTFATTGLTRKDYDQYYRGFSNATLWPVFHYRNDLARYEREEYAGYRRVNAWLSHKL
CCCEEEEECCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKLLEPDDIIWVHDYHLIPFAEALRSEGVRNRIGFFLHIPFPAPQILLNIPPHEELVKSL
HHHCCCCCEEEEECCCCCHHHHHHHHCCCHHHCCEEEECCCCCCEEEEECCCHHHHHHHH
CCYDLLGFQTENDELAFHDYLRRHARGTVSADGHAQAFGRQMRTGVYRIGVFPDEIAEQA
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHH
KRYESRQHVLDLKQSLEGRKLIMSVDRLDYSKGLVERFRAFEALLERSPEWRGNVTLVQI
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
APPTRSDVTTYQDIRQQLEYEAGRINGKYSGLDYTPIRYLNQQYDRWKLMSLFRESQVGF
CCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
VTPLHDGMNLVAKEYVAAQNPDDPGVLVLSMFAGAAAELEGALIVNPHDSIGMCEALQRA
EEECHHHHHHHHHHHHHCCCCCCCCEEHHHHHHCHHHHCCCEEEECCCCCCCHHHHHHHH
LAMPLDERKRRHDIDMQALRKNDLGVWRDSFLRDLRSVPLAGSPSDASGTSTDSAKRTK
HCCCHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GRLIVVSNRVATPTETRGSAGGLAVGVFGALKDAGGVWFGWSGDVVSETVANAGPTLET
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCCCCC
DGSVTFATTGLTRKDYDQYYRGFSNATLWPVFHYRNDLARYEREEYAGYRRVNAWLSHKL
CCCEEEEECCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKLLEPDDIIWVHDYHLIPFAEALRSEGVRNRIGFFLHIPFPAPQILLNIPPHEELVKSL
HHHCCCCCEEEEECCCCCHHHHHHHHCCCHHHCCEEEECCCCCCEEEEECCCHHHHHHHH
CCYDLLGFQTENDELAFHDYLRRHARGTVSADGHAQAFGRQMRTGVYRIGVFPDEIAEQA
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHH
KRYESRQHVLDLKQSLEGRKLIMSVDRLDYSKGLVERFRAFEALLERSPEWRGNVTLVQI
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
APPTRSDVTTYQDIRQQLEYEAGRINGKYSGLDYTPIRYLNQQYDRWKLMSLFRESQVGF
CCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
VTPLHDGMNLVAKEYVAAQNPDDPGVLVLSMFAGAAAELEGALIVNPHDSIGMCEALQRA
EEECHHHHHHHHHHHHHCCCCCCCCEEHHHHHHCHHHHCCCEEEECCCCCCCHHHHHHHH
LAMPLDERKRRHDIDMQALRKNDLGVWRDSFLRDLRSVPLAGSPSDASGTSTDSAKRTK
HCCCHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA