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The map label for this gene is mscL

Identifier: 186475758

GI number: 186475758

Start: 1112708

End: 1113157

Strand: Reverse

Name: mscL

Synonym: Bphy_0993

Alternate gene names: 186475758

Gene position: 1113157-1112708 (Counterclockwise)

Preceding gene: 186475759

Following gene: 186475754

Centisome position: 31.99

GC content: 59.56

Gene sequence:

>450_bases
ATGAGCTTAGTCACGGAATTCAAGGAATTTGCCCTTAAAGGCAACGTAATGGACCTCGCGGTCGGTGTGATCATCGGCGG
CGCGTTCTCGACGATCGTCAACTCCGTCGTCAAGGACCTGATCATGCCCGTCGTTGGCGTGGCGACGGGCGGCCTCGATT
TCTCCAACAAGTTCATCCTGCTCGGGCACATACCCCCGAACTTCAAGGGCAACCCCGATTCGTACAAAGACCTGCAGACG
GCAGGCGTCGCCGCGTTCGGCTATGGCTCGTTCATCACGGTCGCGATCAACTTCGTGATTCTCGCGCTGATCATCTTCAT
GATGGTCAAGTTCATCAACAAGCTGCGCGCCCCGGCACCCGCCGAAGCGGCCGCCCCGCCGCCCACGCCGGAAGACGTGC
TGCTGCTGCGCGAAATTCGCGATTCCCTGAAAAATTCGCCGCGCGTCTGA

Upstream 100 bases:

>100_bases
CGGCCATTTCCATGCGTGATGGCGATCGGACGCTGAATCGTTTCACGCTGCGTCGATCGTTGGCTATTATGTCGCCATCG
ACTTCAAGGAGAACTGCCCG

Downstream 100 bases:

>100_bases
CCGCGGAACACTGAGGCGGCATGGGCGAGGCGCCGCCCCCGAATGACACCAGGGCCTGCCGATCGACACGCCCTTGTCGT
TTTCACCCAACGCGGACGGT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MSLVTEFKEFALKGNVMDLAVGVIIGGAFSTIVNSVVKDLIMPVVGVATGGLDFSNKFILLGHIPPNFKGNPDSYKDLQT
AGVAAFGYGSFITVAINFVILALIIFMMVKFINKLRAPAPAEAAAPPPTPEDVLLLREIRDSLKNSPRV

Sequences:

>Translated_149_residues
MSLVTEFKEFALKGNVMDLAVGVIIGGAFSTIVNSVVKDLIMPVVGVATGGLDFSNKFILLGHIPPNFKGNPDSYKDLQT
AGVAAFGYGSFITVAINFVILALIIFMMVKFINKLRAPAPAEAAAPPPTPEDVLLLREIRDSLKNSPRV
>Mature_148_residues
SLVTEFKEFALKGNVMDLAVGVIIGGAFSTIVNSVVKDLIMPVVGVATGGLDFSNKFILLGHIPPNFKGNPDSYKDLQTA
GVAAFGYGSFITVAINFVILALIIFMMVKFINKLRAPAPAEAAAPPPTPEDVLLLREIRDSLKNSPRV

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=146, Percent_Identity=46.5753424657534, Blast_Score=113, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_BURP8 (B2JGJ7)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001857228.1
- ProteinModelPortal:   B2JGJ7
- SMR:   B2JGJ7
- GeneID:   6242491
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0993
- HOGENOM:   HBG727604
- OMA:   SNKFILL
- ProtClustDB:   PRK13952
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15904; Mature: 15773

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1c5b8638)-; HASH(0x1d4ed934)-; HASH(0x1ce6cf70)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVTEFKEFALKGNVMDLAVGVIIGGAFSTIVNSVVKDLIMPVVGVATGGLDFSNKFIL
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
LGHIPPNFKGNPDSYKDLQTAGVAAFGYGSFITVAINFVILALIIFMMVKFINKLRAPAP
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AEAAAPPPTPEDVLLLREIRDSLKNSPRV
CCCCCCCCCCHHHHHHHHHHHHHCCCCCH
>Mature Secondary Structure 
SLVTEFKEFALKGNVMDLAVGVIIGGAFSTIVNSVVKDLIMPVVGVATGGLDFSNKFIL
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
LGHIPPNFKGNPDSYKDLQTAGVAAFGYGSFITVAINFVILALIIFMMVKFINKLRAPAP
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AEAAAPPPTPEDVLLLREIRDSLKNSPRV
CCCCCCCCCCHHHHHHHHHHHHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA