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The map label for this gene is rimO

Identifier: 186475738

GI number: 186475738

Start: 1087506

End: 1088897

Strand: Direct

Name: rimO

Synonym: Bphy_0973

Alternate gene names: 186475738

Gene position: 1087506-1088897 (Clockwise)

Preceding gene: 186475737

Following gene: 186475739

Centisome position: 31.26

GC content: 62.86

Gene sequence:

>1392_bases
ATGTCGCAGACTTCGATCTCCTCCGCACCGACCGCTTTGACTCCGAAGGTCGGATTCGTCTCACTCGGATGCCCCAAAGC
GCTTGTCGATTCCGAACAGATCATCACCCAACTGCGTGCCGAGGGTTACGAAATCTCCGGCACGTATGACGGCGCGGATC
TCGTCGTCGTGAACACCTGCGGATTCATCGACGAAGCCGTGCAGGAAAGTCTCGACGCGATCGGCGAAGCGCTGAACGAA
AACGGCAAGGTAATCGTCACCGGCTGCCTCGGCGCGAAGCAGAGCGCGAGCGGCTCGAACCTGATCGAGGAAGTGCACCC
GAAGGTGCTCGCTGTGACGGGCCCGCATGCCGTCGGCGAAGTGATGCAGGCCGTGCACAGCCATCTGCCGAAGCCGCACG
ATCCGTTCGTCGACCTCGTGCCCGCTGCGGGCGTCAAGCTCACGCCGCGTCACTATGCGTATCTGAAGATCTCCGAAGGC
TGCAATCACCGCTGCACGTTCTGCATCATCCCGTCGATGCGCGGCGACCTTGTGTCGCGTCCCGTCGCCGAAGTCATGCT
GGAAGCGGAAAACCTGTTCAAGTCGGGCGTGAAGGAACTGCTTGTCATCTCGCAGGACACGAGCGCGTACGGCGTCGACG
TCAAATACCGCACGGGCTTCTGGAACGGCAAACCCATCAAGACGCGCATGACCGACCTCGTCGGCGCGCTCGGCGAACTG
GCCGCGCAGTATGGCGCGTGGGTGCGTCTGCATTATGTGTATCCGTATCCGAGCGTCGACGAAGTGATTCCGATGATGGC
GGAAGGTTCTTTCAAGGGCCACGTGCTGCCGTATCTGGATGTGCCGTTCCAACACGCGCATCCCGAAGTGCTCAAGCGCA
TGAAGCGCCCCGCGAACGCCGAAAAGGTGCTCGAACGCGTGCGTGCATGGCGCGAGATCTGCCCGGACCTGACCATCCGC
AGCACGTTCATCGCGGGCTTTCCCGGTGAGACGCAAGAGCAGTTCGAAACGCTGCTCGACTTCATTCGCGAGGCGGAACT
GGATCGCGTCGGGTGCTTTGCGTATTCGCCCGTCGAAGGCGCGACGGCCAACGAACTCGACGGCGCGCTGCCCGACGAAG
TGCGCGAAGCACGCCGCGCGCGCTTCATGGAAGTCGCGGAACAGGTGTCGGCCAAGCGCATGGCGCGCAAGGTCGGCAAG
ACGCTGAAGGTGCTCGTCGACGAAATCAATCCCGATGGCGGCATTGGCCGCACGGCGGCGGATGCGCCCGAGATCGACGG
CGTCGTCTATATCGCGCCCGCGACGAAGGCGTCGAAGCGCTACAAGGTCGGCGATTTCGTGTCGGTGAAAATCACGGGCG
CGGACGGCCACGACCTGTGGGGCGAGGTCTGA

Upstream 100 bases:

>100_bases
CCGCTCGGTGCGCCTCGTTTGCCGAGATGCCGGCGTTCGGGTTGCATGCCCAAGCCCTCGCACATCGCGTCACTCATCGC
TTCTCGCCGGACCATTCTCT

Downstream 100 bases:

>100_bases
CGCGATGACGTCGAACGCGACCGCCAGCACGCCGTCCATCCTCGCGCTCGGCGAGGCGATGGTCGAATTCAACCAGTCGG
AAAAAAATCAGCCGACATAT

Product: 30S ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSQTSISSAPTALTPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALNE
NGKVIVTGCLGAKQSASGSNLIEEVHPKVLAVTGPHAVGEVMQAVHSHLPKPHDPFVDLVPAAGVKLTPRHYAYLKISEG
CNHRCTFCIIPSMRGDLVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVGALGEL
AAQYGAWVRLHYVYPYPSVDEVIPMMAEGSFKGHVLPYLDVPFQHAHPEVLKRMKRPANAEKVLERVRAWREICPDLTIR
STFIAGFPGETQEQFETLLDFIREAELDRVGCFAYSPVEGATANELDGALPDEVREARRARFMEVAEQVSAKRMARKVGK
TLKVLVDEINPDGGIGRTAADAPEIDGVVYIAPATKASKRYKVGDFVSVKITGADGHDLWGEV

Sequences:

>Translated_463_residues
MSQTSISSAPTALTPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALNE
NGKVIVTGCLGAKQSASGSNLIEEVHPKVLAVTGPHAVGEVMQAVHSHLPKPHDPFVDLVPAAGVKLTPRHYAYLKISEG
CNHRCTFCIIPSMRGDLVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVGALGEL
AAQYGAWVRLHYVYPYPSVDEVIPMMAEGSFKGHVLPYLDVPFQHAHPEVLKRMKRPANAEKVLERVRAWREICPDLTIR
STFIAGFPGETQEQFETLLDFIREAELDRVGCFAYSPVEGATANELDGALPDEVREARRARFMEVAEQVSAKRMARKVGK
TLKVLVDEINPDGGIGRTAADAPEIDGVVYIAPATKASKRYKVGDFVSVKITGADGHDLWGEV
>Mature_462_residues
SQTSISSAPTALTPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALNEN
GKVIVTGCLGAKQSASGSNLIEEVHPKVLAVTGPHAVGEVMQAVHSHLPKPHDPFVDLVPAAGVKLTPRHYAYLKISEGC
NHRCTFCIIPSMRGDLVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVGALGELA
AQYGAWVRLHYVYPYPSVDEVIPMMAEGSFKGHVLPYLDVPFQHAHPEVLKRMKRPANAEKVLERVRAWREICPDLTIRS
TFIAGFPGETQEQFETLLDFIREAELDRVGCFAYSPVEGATANELDGALPDEVREARRARFMEVAEQVSAKRMARKVGKT
LKVLVDEINPDGGIGRTAADAPEIDGVVYIAPATKASKRYKVGDFVSVKITGADGHDLWGEV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=412, Percent_Identity=27.4271844660194, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI28872782, Length=493, Percent_Identity=25.7606490872211, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI28872784, Length=493, Percent_Identity=25.7606490872211, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1787057, Length=448, Percent_Identity=64.2857142857143, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI1786882, Length=465, Percent_Identity=26.2365591397849, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=486, Percent_Identity=23.0452674897119, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71996771, Length=302, Percent_Identity=26.8211920529801, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356207, Length=485, Percent_Identity=27.0103092783505, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI19922432, Length=412, Percent_Identity=27.6699029126214, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_BURP8 (B2JG80)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001857208.1
- ProteinModelPortal:   B2JG80
- GeneID:   6242471
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0973
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   PRK14862
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50348; Mature: 50217

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTSISSAPTALTPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTC
CCCCCCCCCCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCCEEEEECC
GFIDEAVQESLDAIGEALNENGKVIVTGCLGAKQSASGSNLIEEVHPKVLAVTGPHAVGE
CHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHCCCEEEEECCHHHHH
VMQAVHSHLPKPHDPFVDLVPAAGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSR
HHHHHHHHCCCCCCCCHHHHCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHC
PVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVGALGEL
HHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
AAQYGAWVRLHYVYPYPSVDEVIPMMAEGSFKGHVLPYLDVPFQHAHPEVLKRMKRPANA
HHHHCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCH
EKVLERVRAWREICPDLTIRSTFIAGFPGETQEQFETLLDFIREAELDRVGCFAYSPVEG
HHHHHHHHHHHHHCCCCEEEHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
ATANELDGALPDEVREARRARFMEVAEQVSAKRMARKVGKTLKVLVDEINPDGGIGRTAA
CCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DAPEIDGVVYIAPATKASKRYKVGDFVSVKITGADGHDLWGEV
CCCCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SQTSISSAPTALTPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTC
CCCCCCCCCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCCEEEEECC
GFIDEAVQESLDAIGEALNENGKVIVTGCLGAKQSASGSNLIEEVHPKVLAVTGPHAVGE
CHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHCCCEEEEECCHHHHH
VMQAVHSHLPKPHDPFVDLVPAAGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSR
HHHHHHHHCCCCCCCCHHHHCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHC
PVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVGALGEL
HHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
AAQYGAWVRLHYVYPYPSVDEVIPMMAEGSFKGHVLPYLDVPFQHAHPEVLKRMKRPANA
HHHHCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCH
EKVLERVRAWREICPDLTIRSTFIAGFPGETQEQFETLLDFIREAELDRVGCFAYSPVEG
HHHHHHHHHHHHHCCCCEEEHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
ATANELDGALPDEVREARRARFMEVAEQVSAKRMARKVGKTLKVLVDEINPDGGIGRTAA
CCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DAPEIDGVVYIAPATKASKRYKVGDFVSVKITGADGHDLWGEV
CCCCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA