| Definition | Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010622 |
| Length | 3,479,187 |
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The map label for this gene is phbC [H]
Identifier: 186475734
GI number: 186475734
Start: 1082120
End: 1084108
Strand: Direct
Name: phbC [H]
Synonym: Bphy_0969
Alternate gene names: 186475734
Gene position: 1082120-1084108 (Clockwise)
Preceding gene: 186475733
Following gene: 186475735
Centisome position: 31.1
GC content: 61.64
Gene sequence:
>1989_bases ATGGCTGCATCTCACACTTCCTCGACTTCTTCTCGCACGGACACCTCGTCGGACCGCACGCAGCAGCAAGCGAATGCGGC AGGGGTGCACCAATGGTTCGACGCCTGGATGAACGCATGGCGTTCGCTGGGCGCCCAGGCTGCAGCAAACGGCAATCCTT TTAGCGTTCCGTCCATGCCCGACTTCGCGAAAGCGGCGACGGCGCAGGCGGGTTCGCTACCGTTTTCCAATCCCTTGTTC GAGCAAATAGCCAAACAGTTTGCGCAAACGCCTGGCCAACCGAATGGTGAGGCCGCGGGCCAGCCGTTCAACGCCTTGTT CGGACCGGCATTCAATCCGCAGTTCGCACAACAGTTCACGCAGCAGATCGCCGGGTTGAAGATGCCAAGCGCATCGATTC CGCCGAATCGTCTTCAGCAGTTGCAGTCCGACTATTCGCGTGAAGCAATGCAGTTGCTGCAACAGGCAGCACAGGCCACG GCATCGGGCATCGAGCTCAAGGACCGGCGCTTCAGTTCCGAAGCCTGGAAGACGACGCCCATCTACACCCACACGGCTGC GTGGTACCTGCTGAATGCGCGTTATCTGCAGGAACTGGTCGATGCGATCGAAACAGACCAGAAGACGCGCGAGCGCATCC GTTTCGCCGTGCAGCAGTGGACGGCGGCCGCGTCGCCAAGCAACTTCTTTGCGCTGAATCCCGAGGCGCAGAAGACGCTG CTCGAAAGCAATGGCGAAAGCCTGCGGCAGGGCGTGATGAACCTGCTCAGCGACATGCAGCGCGGCAAGATTTCGCAGAC GGACGAGTCGCGCTTCGGCGTCGGCAAGAATCTCGCGATGACGGAAGGTTCCGTCGTATTCGAGAACGAGCTGATGCAGC TGATCCAGTACACGCCGCGCACGGCGACCGTGCACGAACGTCCGCTGCTGATCGTGCCGCCTTGCATCAACAAGTATTAC ATCCTGGATCTTCAGCCCGAGAACTCGCTGGTTGCACATGCGCTCGACTCGGGGCATCAGGTGTTTCTGATTTCGTGGCG CAACGCAGACCAGTCGATCGCCCACAAAACATGGGACGATTACATCGCCGACGGCGTGCTGGCTGCCATCGACACCACGC GTCAGATCAGCGGACGCGAGCAGATCAATACGCTGGGCTTCTGCATCGGTGGCACGCTGCTCGCAACGGCGCTGTCCGTC GCCGCGGCGCGCGGCGAGCATCCCGCTGCGTCGATGACGCTGCTCACCGCGATGCTCGACTTCGCCGACACAGGCGTGCT CGACATCTTCGTCGACGAAGCGCATGTGCAGATGCGCGAGCAGACCATCGGCGGCAAGAACGGTACGCCTCCCGGGCTCA TGCGCGGCATCGAGTTCGCGAACACATTCTCGTTCCTGCGTCCGAACGACCTTGTGTGGAACTACGTCGTCGACAACTAT CTAAAGGGCCGCACGCCGATGCCGTTCGACCTGCTGTACTGGAACAGCGACTCGACGAGCCTGCCCGGTCCGATGTACGT CTGGTATCTGCGCAACACCTACCTCGACAACAAGTTGCGCCAGCCGGGCGCGCTGACCACTTGCGGCGAGAAGGTGGATC TGTCGCGCATCGACGTGCCGACGTTCATCTACGGTTCGCGTGAAGACCACATCGTGCCGTGGCAATCGGCGTATGCGTCG GTGCCGCTGTTGACGGGCCCGCGCAAGTTCGTGTTGGGCGCGTCGGGTCATATTGCCGGTGTGATCAATCCGCCGTCGAA GAACAAGCGCAGTTACTGGTCGGTAGAAGGCGACGACAAGGCGCTGCCTGAGCATCCCGACGACTGGTTCGACACCGCGA CGGAAACGCCTGGCAGCTGGTGGCCCGAGTGGACGAAGTGGCTCGCACAGTACGGCGGCAAGAAGGTGAAGCCGGCAGCG AAAGCAGGTTCGGCCGAGTTCCCGGTGATCGAGCCCGCGCCTGGCCGTTACGTGCAACAGCGCGAATAA
Upstream 100 bases:
>100_bases CCACTGGGCAAGGACGTAACGCGGTCGCGATGCAGTGCTGTTCCCGCGCAAGTACCTGCCTCTCAACCCGTCCGCGAGGA CTTTTCGGTCAAAAGCAGGC
Downstream 100 bases:
>100_bases GCGTCGCGCGGCGGGTTGGATGATTCGCGAGTGGTCGCCGGTGAAGCGCAATGAGCAACCGCCGCCAACGAACACCGGGT GAAGATGGATGGAGTGAGAC
Product: poly(R)-hydroxyalkanoic acid synthase, class I
Products: NA
Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]
Number of amino acids: Translated: 662; Mature: 661
Protein sequence:
>662_residues MAASHTSSTSSRTDTSSDRTQQQANAAGVHQWFDAWMNAWRSLGAQAAANGNPFSVPSMPDFAKAATAQAGSLPFSNPLF EQIAKQFAQTPGQPNGEAAGQPFNALFGPAFNPQFAQQFTQQIAGLKMPSASIPPNRLQQLQSDYSREAMQLLQQAAQAT ASGIELKDRRFSSEAWKTTPIYTHTAAWYLLNARYLQELVDAIETDQKTRERIRFAVQQWTAAASPSNFFALNPEAQKTL LESNGESLRQGVMNLLSDMQRGKISQTDESRFGVGKNLAMTEGSVVFENELMQLIQYTPRTATVHERPLLIVPPCINKYY ILDLQPENSLVAHALDSGHQVFLISWRNADQSIAHKTWDDYIADGVLAAIDTTRQISGREQINTLGFCIGGTLLATALSV AAARGEHPAASMTLLTAMLDFADTGVLDIFVDEAHVQMREQTIGGKNGTPPGLMRGIEFANTFSFLRPNDLVWNYVVDNY LKGRTPMPFDLLYWNSDSTSLPGPMYVWYLRNTYLDNKLRQPGALTTCGEKVDLSRIDVPTFIYGSREDHIVPWQSAYAS VPLLTGPRKFVLGASGHIAGVINPPSKNKRSYWSVEGDDKALPEHPDDWFDTATETPGSWWPEWTKWLAQYGGKKVKPAA KAGSAEFPVIEPAPGRYVQQRE
Sequences:
>Translated_662_residues MAASHTSSTSSRTDTSSDRTQQQANAAGVHQWFDAWMNAWRSLGAQAAANGNPFSVPSMPDFAKAATAQAGSLPFSNPLF EQIAKQFAQTPGQPNGEAAGQPFNALFGPAFNPQFAQQFTQQIAGLKMPSASIPPNRLQQLQSDYSREAMQLLQQAAQAT ASGIELKDRRFSSEAWKTTPIYTHTAAWYLLNARYLQELVDAIETDQKTRERIRFAVQQWTAAASPSNFFALNPEAQKTL LESNGESLRQGVMNLLSDMQRGKISQTDESRFGVGKNLAMTEGSVVFENELMQLIQYTPRTATVHERPLLIVPPCINKYY ILDLQPENSLVAHALDSGHQVFLISWRNADQSIAHKTWDDYIADGVLAAIDTTRQISGREQINTLGFCIGGTLLATALSV AAARGEHPAASMTLLTAMLDFADTGVLDIFVDEAHVQMREQTIGGKNGTPPGLMRGIEFANTFSFLRPNDLVWNYVVDNY LKGRTPMPFDLLYWNSDSTSLPGPMYVWYLRNTYLDNKLRQPGALTTCGEKVDLSRIDVPTFIYGSREDHIVPWQSAYAS VPLLTGPRKFVLGASGHIAGVINPPSKNKRSYWSVEGDDKALPEHPDDWFDTATETPGSWWPEWTKWLAQYGGKKVKPAA KAGSAEFPVIEPAPGRYVQQRE >Mature_661_residues AASHTSSTSSRTDTSSDRTQQQANAAGVHQWFDAWMNAWRSLGAQAAANGNPFSVPSMPDFAKAATAQAGSLPFSNPLFE QIAKQFAQTPGQPNGEAAGQPFNALFGPAFNPQFAQQFTQQIAGLKMPSASIPPNRLQQLQSDYSREAMQLLQQAAQATA SGIELKDRRFSSEAWKTTPIYTHTAAWYLLNARYLQELVDAIETDQKTRERIRFAVQQWTAAASPSNFFALNPEAQKTLL ESNGESLRQGVMNLLSDMQRGKISQTDESRFGVGKNLAMTEGSVVFENELMQLIQYTPRTATVHERPLLIVPPCINKYYI LDLQPENSLVAHALDSGHQVFLISWRNADQSIAHKTWDDYIADGVLAAIDTTRQISGREQINTLGFCIGGTLLATALSVA AARGEHPAASMTLLTAMLDFADTGVLDIFVDEAHVQMREQTIGGKNGTPPGLMRGIEFANTFSFLRPNDLVWNYVVDNYL KGRTPMPFDLLYWNSDSTSLPGPMYVWYLRNTYLDNKLRQPGALTTCGEKVDLSRIDVPTFIYGSREDHIVPWQSAYASV PLLTGPRKFVLGASGHIAGVINPPSKNKRSYWSVEGDDKALPEHPDDWFDTATETPGSWWPEWTKWLAQYGGKKVKPAAK AGSAEFPVIEPAPGRYVQQRE
Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]
COG id: COG3243
COG function: function code I; Poly(3-hydroxyalkanoate) synthetase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the PHA/PHB synthase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000073 - InterPro: IPR010963 - InterPro: IPR010941 [H]
Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]
EC number: NA
Molecular weight: Translated: 73192; Mature: 73061
Theoretical pI: Translated: 6.11; Mature: 6.11
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAASHTSSTSSRTDTSSDRTQQQANAAGVHQWFDAWMNAWRSLGAQAAANGNPFSVPSMP CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCC DFAKAATAQAGSLPFSNPLFEQIAKQFAQTPGQPNGEAAGQPFNALFGPAFNPQFAQQFT HHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHH QQIAGLKMPSASIPPNRLQQLQSDYSREAMQLLQQAAQATASGIELKDRRFSSEAWKTTP HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCC IYTHTAAWYLLNARYLQELVDAIETDQKTRERIRFAVQQWTAAASPSNFFALNPEAQKTL CEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHH LESNGESLRQGVMNLLSDMQRGKISQTDESRFGVGKNLAMTEGSVVFENELMQLIQYTPR HHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEECCCCEEEHHHHHHHHHCCCC TATVHERPLLIVPPCINKYYILDLQPENSLVAHALDSGHQVFLISWRNADQSIAHKTWDD CCEECCCCEEEECCCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH YIADGVLAAIDTTRQISGREQINTLGFCIGGTLLATALSVAAARGEHPAASMTLLTAMLD HHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH FADTGVLDIFVDEAHVQMREQTIGGKNGTPPGLMRGIEFANTFSFLRPNDLVWNYVVDNY HHCCCEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH LKGRTPMPFDLLYWNSDSTSLPGPMYVWYLRNTYLDNKLRQPGALTTCGEKVDLSRIDVP HCCCCCCCEEEEEECCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC TFIYGSREDHIVPWQSAYASVPLLTGPRKFVLGASGHIAGVINPPSKNKRSYWSVEGDDK EEEECCCCCCCCCHHHHHCCCCEECCCCEEEEECCCCEEEEECCCCCCCCCEEEECCCCC ALPEHPDDWFDTATETPGSWWPEWTKWLAQYGGKKVKPAAKAGSAEFPVIEPAPGRYVQQ CCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCCCCCCCEECCCCCCCCCC RE CC >Mature Secondary Structure AASHTSSTSSRTDTSSDRTQQQANAAGVHQWFDAWMNAWRSLGAQAAANGNPFSVPSMP CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCC DFAKAATAQAGSLPFSNPLFEQIAKQFAQTPGQPNGEAAGQPFNALFGPAFNPQFAQQFT HHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHH QQIAGLKMPSASIPPNRLQQLQSDYSREAMQLLQQAAQATASGIELKDRRFSSEAWKTTP HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCC IYTHTAAWYLLNARYLQELVDAIETDQKTRERIRFAVQQWTAAASPSNFFALNPEAQKTL CEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHH LESNGESLRQGVMNLLSDMQRGKISQTDESRFGVGKNLAMTEGSVVFENELMQLIQYTPR HHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEECCCCEEEHHHHHHHHHCCCC TATVHERPLLIVPPCINKYYILDLQPENSLVAHALDSGHQVFLISWRNADQSIAHKTWDD CCEECCCCEEEECCCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH YIADGVLAAIDTTRQISGREQINTLGFCIGGTLLATALSVAAARGEHPAASMTLLTAMLD HHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH FADTGVLDIFVDEAHVQMREQTIGGKNGTPPGLMRGIEFANTFSFLRPNDLVWNYVVDNY HHCCCEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH LKGRTPMPFDLLYWNSDSTSLPGPMYVWYLRNTYLDNKLRQPGALTTCGEKVDLSRIDVP HCCCCCCCEEEEEECCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC TFIYGSREDHIVPWQSAYASVPLLTGPRKFVLGASGHIAGVINPPSKNKRSYWSVEGDDK EEEECCCCCCCCCHHHHHCCCCEECCCCEEEEECCCCEEEEECCCCCCCCCEEEECCCCC ALPEHPDDWFDTATETPGSWWPEWTKWLAQYGGKKVKPAAKAGSAEFPVIEPAPGRYVQQ CCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCCCCCCCEECCCCCCCCCC RE CC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2670936; 1987116 [H]