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The map label for this gene is rumA [H]

Identifier: 186475730

GI number: 186475730

Start: 1077109

End: 1078530

Strand: Direct

Name: rumA [H]

Synonym: Bphy_0965

Alternate gene names: 186475730

Gene position: 1077109-1078530 (Clockwise)

Preceding gene: 186475728

Following gene: 186475732

Centisome position: 30.96

GC content: 63.99

Gene sequence:

>1422_bases
GTGTCCGAAACTGTCCCCCACATTGGTAAAACCCGCTCGCCCAGAAAAGGCAAGCAGGCGCGCGAAGACGCCGCCGGCCC
GTTCAAGGCGCCCGAACTCGACATCGAGTCGCTCGACATGGAAGCGCGCGGCGTCGGCCGCACCGTCACGGAAGACGGCT
CGCCCGGCAAGGTCATCTTTGTCGAAGGCGCGCTGCCCGGCGAACGCGTCACGTACTCGAGCTACCGCAAGAAGCCCAGT
TTCGAACAGGCGCAGGTCGTCGATATTCTTAAAGAGAGCGTGATCCGTACCCGCCCGCAGTGCAAGTTTTTTGGCACCTG
CGGGGGCTGTTCGATGCAGCATCTCGACGTGCGTGCACAAATCGCTGTCAAGGGTCGCGTGCTCGAAGACAATCTGATGC
ACCTGGCCAGGCTGCGTCCGGAAACCGTGTTCCGGCCGATCCACGGTCCGTCCTGGGGCTATCGCTATCGCGCGCGCCTG
ACGGTGCGCCATGTCGCGAAAAAGGGCGGCGTGCTGGTCGGTTTTCACGAGCGCAAGAGCAGTTACGTCGCCGACATGAC
GAGTTGCGAAGTGCTGCCGCCGCATGTTTCCGACATGCTCGTGCCGCTGCGCCACATGGTCGAAGCGCTGTCGATTCGCG
ATCGCATGCCGCAGATCGAACTCGCGGTCGGTGCATCGGTGACGGCGCTCGTGCTGCGCATCCTCGAGCCGATCAACGCC
GCCGACGAACAGCTGCTGCGCGACTTTGCGGATCTGCACAACGTCCAGTTCTGGCTGCAGCCGAAGGGCCCGGATTCCGT
CTATCCGTTCTATCCGCTCGATCGCCAGCTCGATTACACGCTACCCGAGTTCGGCATCCGCATGCCGTTCCGCCCGACGG
ATTTCACGCAGGTCAATCACCAGATCAACCGCGTGCTGGTCGGCCGGGCGCTGCGTCTGCTCGCGCCTGCGAAGACCGAC
CGTGTGCTCGATCTGTTCTGCGGTATCGGCAACTTCACGCTGCCGCTCGCGCGGATTTCCCGCGAAGTGGTGGGCATCGA
AGGCAGCGAGGTGCTGACCTCGCGCGCACTCGACAACGCGAAGGAAAACGGCGTAGACAGCCACACGTCGTTTGCGTGCC
GCAACCTGTTCGAAGTCACGGCCGACGACATCCGCGCGCTAGGCCACTTCGACAAGTATCTGATCGATCCGCCGCGCGAA
GGCGCGCTCGCCGTGTCGAAGGCGCTCGCCGACATCGCACAGAGCGGCGAAGGGCGGCTGCCGACACGCATCGTCTACGT
GTCGTGCAATCCCGCGACGCTCGCGCGCGACGCGGGGCTGCTGGTGCACGAGGCCGGTTATCGCTTGAAGGGCGCGGGTG
TGGTGAACATGTTCCCGCACACGTCGCACGTCGAATCCATCGCACTGTTCGAACGGGACTGA

Upstream 100 bases:

>100_bases
CAGCTGATCAGTCGCATCGTCAAATCTCCGTCGGTGTGTGTCGTTCGTCTACAATCTCGTGCTTGGTCCCACTTTTACAC
AGTCTGTCAGGAAGTCGCTG

Downstream 100 bases:

>100_bases
GGCGCAAGGCCTGTACTTGAAGGCGTTCGCACAGCGTCAAGCGGGCGCCGCAAAGAACAACGCCACGGTCGGCACCGTGG
CGTTGTTCTTTGATGAAGAG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSETVPHIGKTRSPRKGKQAREDAAGPFKAPELDIESLDMEARGVGRTVTEDGSPGKVIFVEGALPGERVTYSSYRKKPS
FEQAQVVDILKESVIRTRPQCKFFGTCGGCSMQHLDVRAQIAVKGRVLEDNLMHLARLRPETVFRPIHGPSWGYRYRARL
TVRHVAKKGGVLVGFHERKSSYVADMTSCEVLPPHVSDMLVPLRHMVEALSIRDRMPQIELAVGASVTALVLRILEPINA
ADEQLLRDFADLHNVQFWLQPKGPDSVYPFYPLDRQLDYTLPEFGIRMPFRPTDFTQVNHQINRVLVGRALRLLAPAKTD
RVLDLFCGIGNFTLPLARISREVVGIEGSEVLTSRALDNAKENGVDSHTSFACRNLFEVTADDIRALGHFDKYLIDPPRE
GALAVSKALADIAQSGEGRLPTRIVYVSCNPATLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIALFERD

Sequences:

>Translated_473_residues
MSETVPHIGKTRSPRKGKQAREDAAGPFKAPELDIESLDMEARGVGRTVTEDGSPGKVIFVEGALPGERVTYSSYRKKPS
FEQAQVVDILKESVIRTRPQCKFFGTCGGCSMQHLDVRAQIAVKGRVLEDNLMHLARLRPETVFRPIHGPSWGYRYRARL
TVRHVAKKGGVLVGFHERKSSYVADMTSCEVLPPHVSDMLVPLRHMVEALSIRDRMPQIELAVGASVTALVLRILEPINA
ADEQLLRDFADLHNVQFWLQPKGPDSVYPFYPLDRQLDYTLPEFGIRMPFRPTDFTQVNHQINRVLVGRALRLLAPAKTD
RVLDLFCGIGNFTLPLARISREVVGIEGSEVLTSRALDNAKENGVDSHTSFACRNLFEVTADDIRALGHFDKYLIDPPRE
GALAVSKALADIAQSGEGRLPTRIVYVSCNPATLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIALFERD
>Mature_472_residues
SETVPHIGKTRSPRKGKQAREDAAGPFKAPELDIESLDMEARGVGRTVTEDGSPGKVIFVEGALPGERVTYSSYRKKPSF
EQAQVVDILKESVIRTRPQCKFFGTCGGCSMQHLDVRAQIAVKGRVLEDNLMHLARLRPETVFRPIHGPSWGYRYRARLT
VRHVAKKGGVLVGFHERKSSYVADMTSCEVLPPHVSDMLVPLRHMVEALSIRDRMPQIELAVGASVTALVLRILEPINAA
DEQLLRDFADLHNVQFWLQPKGPDSVYPFYPLDRQLDYTLPEFGIRMPFRPTDFTQVNHQINRVLVGRALRLLAPAKTDR
VLDLFCGIGNFTLPLARISREVVGIEGSEVLTSRALDNAKENGVDSHTSFACRNLFEVTADDIRALGHFDKYLIDPPREG
ALAVSKALADIAQSGEGRLPTRIVYVSCNPATLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIALFERD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=200, Percent_Identity=30.5, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI51173878, Length=200, Percent_Identity=30.5, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789148, Length=445, Percent_Identity=36.4044943820225, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI1787083, Length=395, Percent_Identity=25.0632911392405, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790403, Length=368, Percent_Identity=25, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24666579, Length=209, Percent_Identity=32.0574162679426, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 52518; Mature: 52387

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETVPHIGKTRSPRKGKQAREDAAGPFKAPELDIESLDMEARGVGRTVTEDGSPGKVIF
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHCCCHHCCCCCEECCCCCCCEEEE
VEGALPGERVTYSSYRKKPSFEQAQVVDILKESVIRTRPQCKFFGTCGGCSMQHLDVRAQ
EECCCCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECEEEE
IAVKGRVLEDNLMHLARLRPETVFRPIHGPSWGYRYRARLTVRHVAKKGGVLVGFHERKS
EEEECEEHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEEHHHHHHHHHCCEEEEEHHCCC
SYVADMTSCEVLPPHVSDMLVPLRHMVEALSIRDRMPQIELAVGASVTALVLRILEPINA
CHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCH
ADEQLLRDFADLHNVQFWLQPKGPDSVYPFYPLDRQLDYTLPEFGIRMPFRPTDFTQVNH
HHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHH
QINRVLVGRALRLLAPAKTDRVLDLFCGIGNFTLPLARISREVVGIEGSEVLTSRALDNA
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
KENGVDSHTSFACRNLFEVTADDIRALGHFDKYLIDPPREGALAVSKALADIAQSGEGRL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCC
PTRIVYVSCNPATLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIALFERD
CEEEEEEECCCHHHHHCCCEEEECCCCEEECCCEEEECCCCCCHHHEEEEECC
>Mature Secondary Structure 
SETVPHIGKTRSPRKGKQAREDAAGPFKAPELDIESLDMEARGVGRTVTEDGSPGKVIF
CCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHCCCHHCCCCCEECCCCCCCEEEE
VEGALPGERVTYSSYRKKPSFEQAQVVDILKESVIRTRPQCKFFGTCGGCSMQHLDVRAQ
EECCCCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECEEEE
IAVKGRVLEDNLMHLARLRPETVFRPIHGPSWGYRYRARLTVRHVAKKGGVLVGFHERKS
EEEECEEHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEEHHHHHHHHHCCEEEEEHHCCC
SYVADMTSCEVLPPHVSDMLVPLRHMVEALSIRDRMPQIELAVGASVTALVLRILEPINA
CHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCH
ADEQLLRDFADLHNVQFWLQPKGPDSVYPFYPLDRQLDYTLPEFGIRMPFRPTDFTQVNH
HHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHH
QINRVLVGRALRLLAPAKTDRVLDLFCGIGNFTLPLARISREVVGIEGSEVLTSRALDNA
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
KENGVDSHTSFACRNLFEVTADDIRALGHFDKYLIDPPREGALAVSKALADIAQSGEGRL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCC
PTRIVYVSCNPATLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIALFERD
CEEEEEEECCCHHHHHCCCEEEECCCCEEECCCEEEECCCCCCHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA