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The map label for this gene is rpoS [H]

Identifier: 186475727

GI number: 186475727

Start: 1074146

End: 1075240

Strand: Direct

Name: rpoS [H]

Synonym: Bphy_0962

Alternate gene names: 186475727

Gene position: 1074146-1075240 (Clockwise)

Preceding gene: 186475726

Following gene: 186475728

Centisome position: 30.87

GC content: 64.02

Gene sequence:

>1095_bases
ATGCCGAAATCGAAGCGCCGCCCATCGCAAGCAGAGACTGAGTCCCTCAGCCGTGCCACGCCTGTTTCGGTGGACGACGC
CGGCGCTTCCGACGCCGACGACGACAATGTCGACGAACTCGACAACAGCAACGACGCCGACGAGCGCGGCTCGCGCGGCG
ACGAGGAACCGGATTCGCGTGAAGGCGCAAACGATTCCGCGCCCGACGCCGACGATTTCCGCGCGCTGCTGCAGGCCGAA
CTCACGGCCGACACGATCCAGCACTACCTGAACCGGATCAGTGTGAAGCCGCTGCTCACCGTCGAGGAAGAGCAGCGCTA
TTCGCGTCTCGCGAAGGCGGGCGAGTTCGAAGCGCGCCAGGTGATGATCGAGCGGAACCTGCGGCTCGTGGTCAGCATCG
CGAAAGGCTATCTGAACCGCGGCGTGCCGCTGCTCGATCTGATCGAAGAGGGCAACCTCGGGCTGATGCACGCCATCGAA
AAGTTCGATCCGACGCGCGGCTTCCGCTTCTCGACTTATGCGACATGGTGGATACGCCAGAGCATCGAGCGCGCGATCAT
GAACCAGGCGCGCACCGTGCGTCTGCCCGTGCACGTGATTCGCGAGCTGAACCAGGTGCTGCGCGCAAAGCGCCACCTCG
AGAAGAACTCGATGAATTCGGGCGAGGCGGCCGAGCGCCGCGACGCGAGTATCGACGACATCGCGTACCTGACAGGCAAG
ACGACGGATGAAGTCACCGATATCCTCGCGCTGAACGAGCACACGGCGTCGCTCGACGCGCCGCTCGATCTCGATCCCGC
CAGCAGTCTGCTCGACCTGCTGTCTGACGATCAGAGCCAGTCGCCCGATGCCGAGGTCCAGCATCGCGAGCTCGAGACGC
TGACGCGCGCGTGGCTCGCCCGGCTGTCAGACAAGCACCGGCATGTGATCGAGCGCCGCTTTGGCCTGAATCACATCGAA
CCGGCCACGCTCGAAGAACTCGCCGACGAAATGGGCCTCACGCGTGAGCGTGTGCGCCAGATCCAGCAAGAGGCGCTGGT
GCGATTGAAGCGCTTCTTTGCTTCCAATGGCGTACGCAAGGACGCCGTTCTCTAG

Upstream 100 bases:

>100_bases
GATTCGGACCGCGTGATGTTGCATTTCGAAGTTCGCCGCCAGGGTAAGCCTGTCGACCCAATGAAGTATTTGCCGCCGCA
ATAAGCCAAGCGATACGACC

Downstream 100 bases:

>100_bases
AAACTGATGACACCCATTCTGGTTTTCGACATCGAGACGATTCCCGATGTCGCTGGCATTCGCCGGCTTGAAAGTCTTCC
CGCAGCGATGTCCGACGAAG

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MPKSKRRPSQAETESLSRATPVSVDDAGASDADDDNVDELDNSNDADERGSRGDEEPDSREGANDSAPDADDFRALLQAE
LTADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIE
KFDPTRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMNSGEAAERRDASIDDIAYLTGK
TTDEVTDILALNEHTASLDAPLDLDPASSLLDLLSDDQSQSPDAEVQHRELETLTRAWLARLSDKHRHVIERRFGLNHIE
PATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL

Sequences:

>Translated_364_residues
MPKSKRRPSQAETESLSRATPVSVDDAGASDADDDNVDELDNSNDADERGSRGDEEPDSREGANDSAPDADDFRALLQAE
LTADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIE
KFDPTRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMNSGEAAERRDASIDDIAYLTGK
TTDEVTDILALNEHTASLDAPLDLDPASSLLDLLSDDQSQSPDAEVQHRELETLTRAWLARLSDKHRHVIERRFGLNHIE
PATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL
>Mature_363_residues
PKSKRRPSQAETESLSRATPVSVDDAGASDADDDNVDELDNSNDADERGSRGDEEPDSREGANDSAPDADDFRALLQAEL
TADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEK
FDPTRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMNSGEAAERRDASIDDIAYLTGKT
TDEVTDILALNEHTASLDAPLDLDPASSLLDLLSDDQSQSPDAEVQHRELETLTRAWLARLSDKHRHVIERRFGLNHIEP
ATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=281, Percent_Identity=50.5338078291815, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI1789448, Length=264, Percent_Identity=38.2575757575758, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1789871, Length=276, Percent_Identity=28.2608695652174, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 41095; Mature: 40964

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSKRRPSQAETESLSRATPVSVDDAGASDADDDNVDELDNSNDADERGSRGDEEPDSR
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHCCCCCCCCCCCC
EGANDSAPDADDFRALLQAELTADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCHHHHH
VMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPTRGFRFSTYATWWIRQ
HHHHHCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHH
SIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMNSGEAAERRDASIDDIAYLTGK
HHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHCCC
TTDEVTDILALNEHTASLDAPLDLDPASSLLDLLSDDQSQSPDAEVQHRELETLTRAWLA
CHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
RLSDKHRHVIERRFGLNHIEPATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
DAVL
HCCC
>Mature Secondary Structure 
PKSKRRPSQAETESLSRATPVSVDDAGASDADDDNVDELDNSNDADERGSRGDEEPDSR
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHCCCCCCCCCCCC
EGANDSAPDADDFRALLQAELTADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCHHHHH
VMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPTRGFRFSTYATWWIRQ
HHHHHCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHH
SIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMNSGEAAERRDASIDDIAYLTGK
HHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHCCC
TTDEVTDILALNEHTASLDAPLDLDPASSLLDLLSDDQSQSPDAEVQHRELETLTRAWLA
CHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
RLSDKHRHVIERRFGLNHIEPATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
DAVL
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]