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The map label for this gene is pit [H]

Identifier: 186475682

GI number: 186475682

Start: 1017947

End: 1018957

Strand: Direct

Name: pit [H]

Synonym: Bphy_0917

Alternate gene names: 186475682

Gene position: 1017947-1018957 (Clockwise)

Preceding gene: 186475681

Following gene: 186475695

Centisome position: 29.26

GC content: 64.49

Gene sequence:

>1011_bases
ATGCATTCGATACAACTCGCCATCTGGGCGGTCGCGACGCTCGTCGTCGTCGCGCTCGTCTTCGACTTCATGAACGGTTT
CCACGACGCAGCGAACTCGATCGCGACCGTCGTCTCGACGGGCGTGCTGAAGCCGCAACAGGCGGTCGCCTTCGCGGCCG
CGTTCAACGTCATCGCGTACTTTGTTTTTCACCTGAAGGTCGCACAGACGGTGGGCAAGGGCACGATCGACCCCGGGATC
GTCGATCACTACGTCGTGTTCGGCGCACTGGTCGGCGCGATCGGCTGGAACATCGTGACCTGGTACTACGGCATTCCGTC
GAGTTCGTCGCATGCGCTGATTGGCGGGCTGGTGGGCTCCGCGCTTGCGAAATCGGGCTGGAGCGCGCTGAACTGGGACG
GTCTGCTGAAGACGATCGCGTTCATTTTCGTGTCGCCGCTGCTCGGCTTCGTGCTCGGCTCGTTCTTCATGCTGCTGGTG
TCGTGGATCTATTTCCGCACGCCGCCGAGCAAGGTCGACCGGCGCTTCCGCCGTCTGCAACTGCTGTCGGCCGGGTTGTA
CAGCCTCGGTCACGGCGGCAACGATGCCCAAAAGACCATCGGCATCATCTGGATGCTGCTGATCGCAACGGGCTATGCAT
CGTCAACGGCCGACGCGCCGCCGCTGTGGGTGATCGGCGGGTGTTACCTGTCGATGGGCCTCGGCACGCTGTTCGGCGGC
TGGCGGATCGTGCGCACGATGGGGCAGAAGATCACGAAACTCAAGCCGGTGGGCGGGTTCTGTGCCGAGGCGGGCGGTGC
GATCACGCTGTTCCTCGCGTCGTTCCTCGGCATCCCCGTGTCCACCACGCATACCATTACCGGCGCGATCGTCGGCGTCG
GCGCGACGCAGAAGCTGTCTGCCGTCCGCTGGGGCGTCGCGGGCAATATCGTGTGGGCCTGGGTGCTGACGATCCCCGCA
TCCGCCGTATTAGCGGCGGCCGGCTGGTGGCTCGGCCACCGCTTCCTTTAA

Upstream 100 bases:

>100_bases
TGATCAAACTGAAGGCAATTTACGAGTTGCTCGAGTCGATCACCGACAAATGCGAGGACGTGGCGAACATCATCGAAGGC
ATCGTGCTGGAAAACGCGTA

Downstream 100 bases:

>100_bases
GCCGCGATACAGCACGCCGCCGTCAGATCAGCGGCGTGCTGCCTCCATCCATCGGGACGATCGCGCCCGTTACGTAGCTC
GCGCGGCGACTCGCCAGAAA

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MHSIQLAIWAVATLVVVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVAFAAAFNVIAYFVFHLKVAQTVGKGTIDPGI
VDHYVVFGALVGAIGWNIVTWYYGIPSSSSHALIGGLVGSALAKSGWSALNWDGLLKTIAFIFVSPLLGFVLGSFFMLLV
SWIYFRTPPSKVDRRFRRLQLLSAGLYSLGHGGNDAQKTIGIIWMLLIATGYASSTADAPPLWVIGGCYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAEAGGAITLFLASFLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWVLTIPA
SAVLAAAGWWLGHRFL

Sequences:

>Translated_336_residues
MHSIQLAIWAVATLVVVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVAFAAAFNVIAYFVFHLKVAQTVGKGTIDPGI
VDHYVVFGALVGAIGWNIVTWYYGIPSSSSHALIGGLVGSALAKSGWSALNWDGLLKTIAFIFVSPLLGFVLGSFFMLLV
SWIYFRTPPSKVDRRFRRLQLLSAGLYSLGHGGNDAQKTIGIIWMLLIATGYASSTADAPPLWVIGGCYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAEAGGAITLFLASFLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWVLTIPA
SAVLAAAGWWLGHRFL
>Mature_336_residues
MHSIQLAIWAVATLVVVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVAFAAAFNVIAYFVFHLKVAQTVGKGTIDPGI
VDHYVVFGALVGAIGWNIVTWYYGIPSSSSHALIGGLVGSALAKSGWSALNWDGLLKTIAFIFVSPLLGFVLGSFFMLLV
SWIYFRTPPSKVDRRFRRLQLLSAGLYSLGHGGNDAQKTIGIIWMLLIATGYASSTADAPPLWVIGGCYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAEAGGAITLFLASFLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWVLTIPA
SAVLAAAGWWLGHRFL

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=175, Percent_Identity=38.2857142857143, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI5803173, Length=155, Percent_Identity=37.4193548387097, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1789907, Length=244, Percent_Identity=32.3770491803279, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789360, Length=224, Percent_Identity=32.5892857142857, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17539280, Length=177, Percent_Identity=33.8983050847458, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=29.6296296296296, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI32566716, Length=169, Percent_Identity=30.1775147928994, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71981576, Length=162, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17557328, Length=169, Percent_Identity=32.5443786982249, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17536725, Length=180, Percent_Identity=31.1111111111111, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319773, Length=174, Percent_Identity=32.183908045977, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21356511, Length=189, Percent_Identity=33.8624338624339, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35601; Mature: 35601

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSIQLAIWAVATLVVVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVAFAAAFNVIAY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FVFHLKVAQTVGKGTIDPGIVDHYVVFGALVGAIGWNIVTWYYGIPSSSSHALIGGLVGS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHH
ALAKSGWSALNWDGLLKTIAFIFVSPLLGFVLGSFFMLLVSWIYFRTPPSKVDRRFRRLQ
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLSAGLYSLGHGGNDAQKTIGIIWMLLIATGYASSTADAPPLWVIGGCYLSMGLGTLFGG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHH
WRIVRTMGQKITKLKPVGGFCAEAGGAITLFLASFLGIPVSTTHTITGAIVGVGATQKLS
HHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHEECCCHHHHH
AVRWGVAGNIVWAWVLTIPASAVLAAAGWWLGHRFL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHSIQLAIWAVATLVVVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVAFAAAFNVIAY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FVFHLKVAQTVGKGTIDPGIVDHYVVFGALVGAIGWNIVTWYYGIPSSSSHALIGGLVGS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHH
ALAKSGWSALNWDGLLKTIAFIFVSPLLGFVLGSFFMLLVSWIYFRTPPSKVDRRFRRLQ
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLSAGLYSLGHGGNDAQKTIGIIWMLLIATGYASSTADAPPLWVIGGCYLSMGLGTLFGG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHH
WRIVRTMGQKITKLKPVGGFCAEAGGAITLFLASFLGIPVSTTHTITGAIVGVGATQKLS
HHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHEECCCHHHHH
AVRWGVAGNIVWAWVLTIPASAVLAAAGWWLGHRFL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]