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The map label for this gene is dinF [H]

Identifier: 186475661

GI number: 186475661

Start: 1000872

End: 1002254

Strand: Reverse

Name: dinF [H]

Synonym: Bphy_0896

Alternate gene names: 186475661

Gene position: 1002254-1000872 (Counterclockwise)

Preceding gene: 186475662

Following gene: 186475659

Centisome position: 28.81

GC content: 68.33

Gene sequence:

>1383_bases
TTGAACGATCGCGCCGACCATGCTGCGCCGCCGGATGCCGCGCCCCATGCGGACGCCGCGCCGACGTACTGGCACCGGCG
CGTGCTCACGCTCGCGTTTCCAATCGTCCTCGCCAACCTGACGCAGCCGATACTCGGCGCCGTCGATACGGCCGTCGCCG
GCCATCTGGACAGCGCGTCCTATCTCGGCGGCGTTGCGCTCGGCGGGCTGTTCTTCAGCTTCGTGTTCTGGGGCTTCGGC
TTTCTGCGCATGGGCACGACGGGGCTCGTCGCGCAATCGTTCGGCGCAGACGATCCGGCCGGCCTGCGTTTGAACATCGT
ACGCGCGATGCTGCTCGCCTTTGCGATCGGCGCGATCGTCCTCGCATTGCAGGTTCCGCTGATCGACTTCGCCGTGCGCA
CGATCGGCGGCAGCGACGAAGTTCAGCGCCACGCACGCGCCTACTGCCATGCCCGTATCTGGGCGGCCCCGCTCGCGCTC
GGCAACTATGTCGTGCTGGGCTGGCTGCTCGGCACGCAGCGCGTGCGCGTTGCGCTCGCGACGCAGATCTTTATCAACAC
GGTGAACATCGTCGCCGTGCTGCTCTACGTGTATGCGTTCGACTGGGGCGTGGCAGGCATCGGCGCGGCGACGGCGACAG
CCGATGCACTCGGCTTCGTGTTCGGCGCCGTGCTGCTGTGGCAGATGCGTCCGCGCAACCTGCCCGCGCTCGAACGCGCC
GCGCTGTTCGATGCGTCGGCGCTCGGGCGCCTGGTGGCGATCAACCGCGATATCTTCGTGCGGACGATGTGCCTGCTCGG
CGCTTTCGGCTGGTTCGCGCATCTCGGTGCTAAACAGGGCGACGCGACGCTCGCGTCGAACGCGCTGCTGCTCAACTTCC
AGACCTTCATGGGCTATGGGCTCGACGGCTTTGCACACGCAGCGGAAGCGCTCGTCGGCGCGGCCATCGGCGCGCGCGAT
CGCGAGGCGTTCCGCCAGGCCGTCAAAGCGACGCTGCTCTGGTCGGCGCTCGGCGCGCTCGCTTTCTCGCTGGTTTACTG
GCTGACGGGCCCGTGGATCATCGAACGGCTGACCGATCAAGCCACCGTGCGCGCGACGGCGCAAGCCTACCTGCCGTGGA
CGGCGCTCTCGCCCGTCGTCTCCGTGTGGGGTTTTCTGCTCGACGGCGTGTTCATCGGCGCGACGCGCACGCGCGATCTG
ATGACATCGATGGTCGTGTCGCTCGGCGTGTTTCTTGCCGCTTCGTGGGCGCTCACCGGCACGCACGGCAACCACGGACT
GTGGGCGGCGCTGCTGATCTTCATGGCAGCGCGCGGCCTCACGCTCGCACGCCTGCTGCCTCGCCTCACCCTCGATGTCG
GCCCCGCGTGCGGCGCTCCCTGA

Upstream 100 bases:

>100_bases
TCGAGCGCGTGCAGTCTGAGACGGCAACGGACTTCGCTGCGCGCGATCCCGACCTGTGGGCCGTCGTGGTATCGCCGTGG
GTCTGCGTGCAGGAGCGCGG

Downstream 100 bases:

>100_bases
ACCTGCATGGGACCGCTCGTTGCGTTTATTGAGGCGAAGCGGGCGCGTCGATCATCGACGGCGGACGTGTGTCCGTCGGC
ACGCCATGATAGTCGGCGGG

Product: MATE efflux family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNDRADHAAPPDAAPHADAAPTYWHRRVLTLAFPIVLANLTQPILGAVDTAVAGHLDSASYLGGVALGGLFFSFVFWGFG
FLRMGTTGLVAQSFGADDPAGLRLNIVRAMLLAFAIGAIVLALQVPLIDFAVRTIGGSDEVQRHARAYCHARIWAAPLAL
GNYVVLGWLLGTQRVRVALATQIFINTVNIVAVLLYVYAFDWGVAGIGAATATADALGFVFGAVLLWQMRPRNLPALERA
ALFDASALGRLVAINRDIFVRTMCLLGAFGWFAHLGAKQGDATLASNALLLNFQTFMGYGLDGFAHAAEALVGAAIGARD
REAFRQAVKATLLWSALGALAFSLVYWLTGPWIIERLTDQATVRATAQAYLPWTALSPVVSVWGFLLDGVFIGATRTRDL
MTSMVVSLGVFLAASWALTGTHGNHGLWAALLIFMAARGLTLARLLPRLTLDVGPACGAP

Sequences:

>Translated_460_residues
MNDRADHAAPPDAAPHADAAPTYWHRRVLTLAFPIVLANLTQPILGAVDTAVAGHLDSASYLGGVALGGLFFSFVFWGFG
FLRMGTTGLVAQSFGADDPAGLRLNIVRAMLLAFAIGAIVLALQVPLIDFAVRTIGGSDEVQRHARAYCHARIWAAPLAL
GNYVVLGWLLGTQRVRVALATQIFINTVNIVAVLLYVYAFDWGVAGIGAATATADALGFVFGAVLLWQMRPRNLPALERA
ALFDASALGRLVAINRDIFVRTMCLLGAFGWFAHLGAKQGDATLASNALLLNFQTFMGYGLDGFAHAAEALVGAAIGARD
REAFRQAVKATLLWSALGALAFSLVYWLTGPWIIERLTDQATVRATAQAYLPWTALSPVVSVWGFLLDGVFIGATRTRDL
MTSMVVSLGVFLAASWALTGTHGNHGLWAALLIFMAARGLTLARLLPRLTLDVGPACGAP
>Mature_460_residues
MNDRADHAAPPDAAPHADAAPTYWHRRVLTLAFPIVLANLTQPILGAVDTAVAGHLDSASYLGGVALGGLFFSFVFWGFG
FLRMGTTGLVAQSFGADDPAGLRLNIVRAMLLAFAIGAIVLALQVPLIDFAVRTIGGSDEVQRHARAYCHARIWAAPLAL
GNYVVLGWLLGTQRVRVALATQIFINTVNIVAVLLYVYAFDWGVAGIGAATATADALGFVFGAVLLWQMRPRNLPALERA
ALFDASALGRLVAINRDIFVRTMCLLGAFGWFAHLGAKQGDATLASNALLLNFQTFMGYGLDGFAHAAEALVGAAIGARD
REAFRQAVKATLLWSALGALAFSLVYWLTGPWIIERLTDQATVRATAQAYLPWTALSPVVSVWGFLLDGVFIGATRTRDL
MTSMVVSLGVFLAASWALTGTHGNHGLWAALLIFMAARGLTLARLLPRLTLDVGPACGAP

Specific function: Unknown

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790477, Length=429, Percent_Identity=41.7249417249417, Blast_Score=241, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 48911; Mature: 48911

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure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CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503 [H]