Click here to switch to the map view.

The map label for this gene is rlmE [H]

Identifier: 186475643

GI number: 186475643

Start: 981120

End: 981782

Strand: Direct

Name: rlmE [H]

Synonym: Bphy_0878

Alternate gene names: 186475643

Gene position: 981120-981782 (Clockwise)

Preceding gene: 186475641

Following gene: 186475644

Centisome position: 28.2

GC content: 58.97

Gene sequence:

>663_bases
ATGGCAAAAAACAAGTTCAATACGTCGTGGCTGCACGACCACATCAACGATCCGTACGTGAAAATGGCGCAGCGGGAGGG
TTATCGCGCCCGCGCCGCCTACAAGCTGAAGGAAATCGACGAGCAGGACAAGCTGATTCGCCCGGGCCAGGTGATCGTTG
ATCTCGGCGCGGCGCCCGGCAGCTGGAGCCAGTATGTGCGCAACAAGCTTTCGCAGGGCAAACACCGCGACGCGCAGCGT
GAAGGCGGCATCGACGGCACCATCATTGCGCTTGACCTGCTGCCGATGGAGCCGATCGCCGACGTGCACTTCATTCAGGG
CGACTTCCGCGAAGACAATGTCCTGGCGCAACTGGAAGAAGTGGTGGGAGATCGCGATGTCGATCTTGTAATTTCGGATA
TGGCGCCCAACCTGTCAGGGGTGGCGGTGGCCGACGCCGCGCGGATCGAGCACGTGTGCGATCTGGCGCTGGAATTTGCC
CAAAACCACCTGAAACCCGATGGTGCCCTTTTAGTCAAATGCTTTCACGGCAGCGGTTATAGCCAGATTGTCGAAAAGTT
CAAGCATCAGTTCAAGACGGTGGCCGCGCGCAAGCCGAAAGCGTCCCGGGACAAGTCGTCCGAGACGTTCATTTTGGGGA
GGCATCTGAAGCGGCCTCGTTGA

Upstream 100 bases:

>100_bases
AGAACCACGGCGCGGACACGGCCTTTGGCTGCTGCCGCGCGAATTAAACGCGTATTATCCGCTAAAAGCGCGGCATTACG
TAGCAAGAGTTCAACGTTTA

Downstream 100 bases:

>100_bases
ACCGGGTGCAGCGATGTGTGAGCCCGCATTTCCGGCACGCCGGAAAATAAGAGCGCTGAGGTTGCGCGGTACGCTATTTA
TGCGGTAGCGAATGCTTATG

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAKNKFNTSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPGSWSQYVRNKLSQGKHRDAQR
EGGIDGTIIALDLLPMEPIADVHFIQGDFREDNVLAQLEEVVGDRDVDLVISDMAPNLSGVAVADAARIEHVCDLALEFA
QNHLKPDGALLVKCFHGSGYSQIVEKFKHQFKTVAARKPKASRDKSSETFILGRHLKRPR

Sequences:

>Translated_220_residues
MAKNKFNTSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPGSWSQYVRNKLSQGKHRDAQR
EGGIDGTIIALDLLPMEPIADVHFIQGDFREDNVLAQLEEVVGDRDVDLVISDMAPNLSGVAVADAARIEHVCDLALEFA
QNHLKPDGALLVKCFHGSGYSQIVEKFKHQFKTVAARKPKASRDKSSETFILGRHLKRPR
>Mature_219_residues
AKNKFNTSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPGSWSQYVRNKLSQGKHRDAQRE
GGIDGTIIALDLLPMEPIADVHFIQGDFREDNVLAQLEEVVGDRDVDLVISDMAPNLSGVAVADAARIEHVCDLALEFAQ
NHLKPDGALLVKCFHGSGYSQIVEKFKHQFKTVAARKPKASRDKSSETFILGRHLKRPR

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=210, Percent_Identity=34.2857142857143, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI194097365, Length=196, Percent_Identity=29.5918367346939, Blast_Score=105, Evalue=2e-23,
Organism=Homo sapiens, GI29029591, Length=203, Percent_Identity=31.5270935960591, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI29029589, Length=203, Percent_Identity=31.5270935960591, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI7110661, Length=203, Percent_Identity=31.5270935960591, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1789569, Length=206, Percent_Identity=45.1456310679612, Blast_Score=194, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17553474, Length=216, Percent_Identity=32.4074074074074, Blast_Score=110, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17554650, Length=203, Percent_Identity=32.0197044334975, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71987550, Length=203, Percent_Identity=30.5418719211823, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71987561, Length=203, Percent_Identity=30.5418719211823, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17553860, Length=203, Percent_Identity=30.5418719211823, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=205, Percent_Identity=31.7073170731707, Blast_Score=110, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319535, Length=198, Percent_Identity=32.3232323232323, Blast_Score=108, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321302, Length=111, Percent_Identity=37.8378378378378, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21356387, Length=217, Percent_Identity=32.7188940092166, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24647580, Length=204, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648639, Length=203, Percent_Identity=32.0197044334975, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI18859957, Length=198, Percent_Identity=28.7878787878788, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 24678; Mature: 24547

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNKFNTSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPG
CCCCCCCCHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYVRNKLSQGKHRDAQREGGIDGTIIALDLLPMEPIADVHFIQGDFREDNVLAQLEE
CHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEEECCCCCCCCCCEECCCCCCCHHHHHHHH
VVGDRDVDLVISDMAPNLSGVAVADAARIEHVCDLALEFAQNHLKPDGALLVKCFHGSGY
HHCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
SQIVEKFKHQFKTVAARKPKASRDKSSETFILGRHLKRPR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
AKNKFNTSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPG
CCCCCCCHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYVRNKLSQGKHRDAQREGGIDGTIIALDLLPMEPIADVHFIQGDFREDNVLAQLEE
CHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEEECCCCCCCCCCEECCCCCCCHHHHHHHH
VVGDRDVDLVISDMAPNLSGVAVADAARIEHVCDLALEFAQNHLKPDGALLVKCFHGSGY
HHCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
SQIVEKFKHQFKTVAARKPKASRDKSSETFILGRHLKRPR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA