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The map label for this gene is greA [H]

Identifier: 186475640

GI number: 186475640

Start: 979137

End: 979613

Strand: Direct

Name: greA [H]

Synonym: Bphy_0875

Alternate gene names: 186475640

Gene position: 979137-979613 (Clockwise)

Preceding gene: 186475639

Following gene: 186475641

Centisome position: 28.14

GC content: 61.43

Gene sequence:

>477_bases
ATGAGCACTATTCCATTGACGAAGCGCGGTGCAGATCAGCTGCGCGACGAATTGCAGCGTTTGAAATCGGTTGAGCGTCC
CGCTGTCATCAATTCCATCGCGGAAGCGCGTGCCCAAGGCGATCTGTCGGAAAACGCCGAGTACGACGCTGCGAAAGAAA
AGCAGGGCTTTATCGAAGGCCGCATCGCCGAGATCGAATCGAAGCTGGCGGCCGCGCAGATCATCGACCCCTCCGCGCTC
GACGCCGACGGTCGCGTGGTGTTCGCCGCGACGGTCGATCTGGAAGATCTGGATTCGGGCGATTCCGTTACCTATCAAAT
CGTCGGCGACGACGAAGCGGATCTCGAGCACGGCCTGATCTCGGTCAGCTCGCCGATCGCCCGTGCGCTGATCGGCAAGT
CGGAAGGCGACGTTGCGGCCGTGCAGGCGCCGAGCGGCGTGCGCGAGTACGAAATCATCGCGGTCCGCTATATCTGA

Upstream 100 bases:

>100_bases
AAGGTGTCAGATCGGGCTTTGTGCCACGTTTGCGCACCGTCGGGGATGCACTTAACCGCGGTGATTTTTTTTGCGGCTGT
TTTTTGACAAAGAGTTGTTT

Downstream 100 bases:

>100_bases
GGCGGGTGCCGTGTCTTCGATGCCGCATCGCCTGTTCCGCCTGTTGACGGTCGTGTGGGTCGGCAGTGTGCTGACCATCG
GCTATGCGGTTGCACCTGTG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSTIPLTKRGADQLRDELQRLKSVERPAVINSIAEARAQGDLSENAEYDAAKEKQGFIEGRIAEIESKLAAAQIIDPSAL
DADGRVVFAATVDLEDLDSGDSVTYQIVGDDEADLEHGLISVSSPIARALIGKSEGDVAAVQAPSGVREYEIIAVRYI

Sequences:

>Translated_158_residues
MSTIPLTKRGADQLRDELQRLKSVERPAVINSIAEARAQGDLSENAEYDAAKEKQGFIEGRIAEIESKLAAAQIIDPSAL
DADGRVVFAATVDLEDLDSGDSVTYQIVGDDEADLEHGLISVSSPIARALIGKSEGDVAAVQAPSGVREYEIIAVRYI
>Mature_157_residues
STIPLTKRGADQLRDELQRLKSVERPAVINSIAEARAQGDLSENAEYDAAKEKQGFIEGRIAEIESKLAAAQIIDPSALD
ADGRVVFAATVDLEDLDSGDSVTYQIVGDDEADLEHGLISVSSPIARALIGKSEGDVAAVQAPSGVREYEIIAVRYI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=58.2278481012658, Blast_Score=189, Evalue=6e-50,
Organism=Escherichia coli, GI87082261, Length=158, Percent_Identity=36.0759493670886, Blast_Score=96, Evalue=9e-22,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 16882; Mature: 16751

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIPLTKRGADQLRDELQRLKSVERPAVINSIAEARAQGDLSENAEYDAAKEKQGFIEG
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHH
RIAEIESKLAAAQIIDPSALDADGRVVFAATVDLEDLDSGDSVTYQIVGDDEADLEHGLI
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECHHHCCCCCCEEEEEECCCCCCHHHCEE
SVSSPIARALIGKSEGDVAAVQAPSGVREYEIIAVRYI
ECCHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEEC
>Mature Secondary Structure 
STIPLTKRGADQLRDELQRLKSVERPAVINSIAEARAQGDLSENAEYDAAKEKQGFIEG
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHH
RIAEIESKLAAAQIIDPSALDADGRVVFAATVDLEDLDSGDSVTYQIVGDDEADLEHGLI
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECHHHCCCCCCEEEEEECCCCCCHHHCEE
SVSSPIARALIGKSEGDVAAVQAPSGVREYEIIAVRYI
ECCHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]