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The map label for this gene is carA [H]

Identifier: 186475638

GI number: 186475638

Start: 974454

End: 975608

Strand: Direct

Name: carA [H]

Synonym: Bphy_0873

Alternate gene names: 186475638

Gene position: 974454-975608 (Clockwise)

Preceding gene: 186475637

Following gene: 186475639

Centisome position: 28.01

GC content: 62.77

Gene sequence:

>1155_bases
GTGTTGCCGTCATTTTCTTCCGCTCTGCTCGCGCTCGCCGACGGCACGGTCTTTCGTGGTTACTCGATCGGCGCGCCCGG
CCATACGATCGGCGAAGTCGTCTTCAATACCGCCATCACCGGTTATCAGGAAATCCTGACGGACCCCAGTTACGCGCGCC
AGATCGTCACGCTGACGTATCCGCACATCGGCAACGTCGGTGTGAACGCCGAAGATGTCGAAGCTACGAAAGTCCATGCC
GCCGGACTGATCATCCGTGATCTGCCCATTCTCGCGTCGAACTTCCGCATGGAGCGCACGCTCCCCGATTACCTGAAGGC
GGAAGGCGTCGTCGCGATCGCCGGTATCGATACCCGCAAGCTCACGCGCGTGCTGCGCGACAAGGGCGCACAGAACGGCG
CGATTCTCGCAGGGTCGGATGACGAAGCGAAGGCAATCGAACTCGCACGCTCGTTCCCCGGCCTCGCAGGGATGGACCTC
GCGAAGGTCGTGTCGACGCAAAAGCCGTACGAATGGAAGCAGACGGAATGGCGTCTCGGCAGCGGCTACGGCATGCAGAA
CACGCCGCGCTATCGCGTGGTCGCATTCGATTACGGCGTCAAGTACAACATCCTGCGCATGCTCGCCGAACGCGGCTGCC
AGGTCACCGTGCTGCCGGCGCAAGCCTCGGCCGCCGATGCGCTTGCGCTCAATCCGGACGGCGTGTTCCTGTCCAACGGC
CCCGGCGATCCGGAACCGTGCGACTACGCGATCGCCGCCACGCGCGAATTCATCGAACGCGGCATTCCGACGTTCGGCAT
CTGTCTCGGCCATCAAATCATGGGTCTCGCCGTCGGCGCGAAGACGATGAAGATGAAAACGGGCCACCACGGTGCGAACC
ATCCTGTGAAGGATCTGCAAGATGGCCGCGTCGTGATCACGTCGCAAAACCACGGTTTCGCCGTGGACGCCGACACGCTG
CCCGCGAACGCGAAGGTCACGCACGTGTCGCTGTTCGACGGCACGCTGCAAGGCTTCGCGTTGACGGACAAGCCGGCTTT
CTGCTTCCAGGGTCACCCGGAAGCGTCGCCCGGTCCGCATGACATCGCTTATCTGTTCGACCGCTTCACCGCGTTGATGG
ACCAGGCGAAGGGCAATAAGTCGGCGGCGGCATAA

Upstream 100 bases:

>100_bases
ACGCGAGCGAGCCTGCGCGCGCATCCTCAGATGCTGCTGCCGCGGCCCGCACGGTCGGATAGACAGGTCGGCAACAGGGT
GCTCGCCCAAGGAGTCTCCC

Downstream 100 bases:

>100_bases
GCGCAGCTCGCGAACAGCGACGGTAACAAGGCGTGCTCCGCGACGCCGTTCGGCATTGTCCGAACGGCACACCGTCGGCG
TGCCAACAGTAAGAACTCAG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MLPSFSSALLALADGTVFRGYSIGAPGHTIGEVVFNTAITGYQEILTDPSYARQIVTLTYPHIGNVGVNAEDVEATKVHA
AGLIIRDLPILASNFRMERTLPDYLKAEGVVAIAGIDTRKLTRVLRDKGAQNGAILAGSDDEAKAIELARSFPGLAGMDL
AKVVSTQKPYEWKQTEWRLGSGYGMQNTPRYRVVAFDYGVKYNILRMLAERGCQVTVLPAQASAADALALNPDGVFLSNG
PGDPEPCDYAIAATREFIERGIPTFGICLGHQIMGLAVGAKTMKMKTGHHGANHPVKDLQDGRVVITSQNHGFAVDADTL
PANAKVTHVSLFDGTLQGFALTDKPAFCFQGHPEASPGPHDIAYLFDRFTALMDQAKGNKSAAA

Sequences:

>Translated_384_residues
MLPSFSSALLALADGTVFRGYSIGAPGHTIGEVVFNTAITGYQEILTDPSYARQIVTLTYPHIGNVGVNAEDVEATKVHA
AGLIIRDLPILASNFRMERTLPDYLKAEGVVAIAGIDTRKLTRVLRDKGAQNGAILAGSDDEAKAIELARSFPGLAGMDL
AKVVSTQKPYEWKQTEWRLGSGYGMQNTPRYRVVAFDYGVKYNILRMLAERGCQVTVLPAQASAADALALNPDGVFLSNG
PGDPEPCDYAIAATREFIERGIPTFGICLGHQIMGLAVGAKTMKMKTGHHGANHPVKDLQDGRVVITSQNHGFAVDADTL
PANAKVTHVSLFDGTLQGFALTDKPAFCFQGHPEASPGPHDIAYLFDRFTALMDQAKGNKSAAA
>Mature_384_residues
MLPSFSSALLALADGTVFRGYSIGAPGHTIGEVVFNTAITGYQEILTDPSYARQIVTLTYPHIGNVGVNAEDVEATKVHA
AGLIIRDLPILASNFRMERTLPDYLKAEGVVAIAGIDTRKLTRVLRDKGAQNGAILAGSDDEAKAIELARSFPGLAGMDL
AKVVSTQKPYEWKQTEWRLGSGYGMQNTPRYRVVAFDYGVKYNILRMLAERGCQVTVLPAQASAADALALNPDGVFLSNG
PGDPEPCDYAIAATREFIERGIPTFGICLGHQIMGLAVGAKTMKMKTGHHGANHPVKDLQDGRVVITSQNHGFAVDADTL
PANAKVTHVSLFDGTLQGFALTDKPAFCFQGHPEASPGPHDIAYLFDRFTALMDQAKGNKSAAA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=37.696335078534, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI21361331, Length=383, Percent_Identity=37.3368146214099, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI169790915, Length=383, Percent_Identity=37.3368146214099, Blast_Score=226, Evalue=2e-59,
Organism=Escherichia coli, GI1786215, Length=377, Percent_Identity=64.9867374005305, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1789760, Length=133, Percent_Identity=30.0751879699248, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=384, Percent_Identity=38.28125, Blast_Score=244, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6324878, Length=397, Percent_Identity=37.7833753148615, Blast_Score=242, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=396, Percent_Identity=37.1212121212121, Blast_Score=239, Evalue=5e-64,
Organism=Drosophila melanogaster, GI45555749, Length=389, Percent_Identity=40.1028277634961, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24642586, Length=389, Percent_Identity=40.1028277634961, Blast_Score=248, Evalue=7e-66,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41071; Mature: 41071

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSFSSALLALADGTVFRGYSIGAPGHTIGEVVFNTAITGYQEILTDPSYARQIVTLTY
CCCCHHHHHHHHCCCCEEECEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEEC
PHIGNVGVNAEDVEATKVHAAGLIIRDLPILASNFRMERTLPDYLKAEGVVAIAGIDTRK
CCCCCCCCCCCCCHHHHHHECCEEEECCCHHHCCCEECCCCHHHHCCCCEEEEECCCHHH
LTRVLRDKGAQNGAILAGSDDEAKAIELARSFPGLAGMDLAKVVSTQKPYEWKQTEWRLG
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEEC
SGYGMQNTPRYRVVAFDYGVKYNILRMLAERGCQVTVLPAQASAADALALNPDGVFLSNG
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEECCC
PGDPEPCDYAIAATREFIERGIPTFGICLGHQIMGLAVGAKTMKMKTGHHGANHPVKDLQ
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCHHHCC
DGRVVITSQNHGFAVDADTLPANAKVTHVSLFDGTLQGFALTDKPAFCFQGHPEASPGPH
CCEEEEEECCCCEEEECCCCCCCCEEEEEEEECCCCCCEEECCCCCEEECCCCCCCCCHH
DIAYLFDRFTALMDQAKGNKSAAA
HHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLPSFSSALLALADGTVFRGYSIGAPGHTIGEVVFNTAITGYQEILTDPSYARQIVTLTY
CCCCHHHHHHHHCCCCEEECEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEEC
PHIGNVGVNAEDVEATKVHAAGLIIRDLPILASNFRMERTLPDYLKAEGVVAIAGIDTRK
CCCCCCCCCCCCCHHHHHHECCEEEECCCHHHCCCEECCCCHHHHCCCCEEEEECCCHHH
LTRVLRDKGAQNGAILAGSDDEAKAIELARSFPGLAGMDLAKVVSTQKPYEWKQTEWRLG
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEEC
SGYGMQNTPRYRVVAFDYGVKYNILRMLAERGCQVTVLPAQASAADALALNPDGVFLSNG
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEECCC
PGDPEPCDYAIAATREFIERGIPTFGICLGHQIMGLAVGAKTMKMKTGHHGANHPVKDLQ
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCHHHCC
DGRVVITSQNHGFAVDADTLPANAKVTHVSLFDGTLQGFALTDKPAFCFQGHPEASPGPH
CCEEEEEECCCCEEEECCCCCCCCEEEEEEEECCCCCCEEECCCCCEEECCCCCCCCCHH
DIAYLFDRFTALMDQAKGNKSAAA
HHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]