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The map label for this gene is dadA [H]

Identifier: 186475530

GI number: 186475530

Start: 862184

End: 863470

Strand: Reverse

Name: dadA [H]

Synonym: Bphy_0765

Alternate gene names: 186475530

Gene position: 863470-862184 (Counterclockwise)

Preceding gene: 186475533

Following gene: 186475523

Centisome position: 24.82

GC content: 64.8

Gene sequence:

>1287_bases
ATGCGAGTCGTCGTTCTGGGCAGTGGCGTCGTCGGGGTAACGAGCGCCTATTATCTTGCGCGCGCGGGTCATGAAGTCAC
CGTGATCGACCGCGAGGCGGGTCCTGCGCTCGAAACGAGCTTTGCGAACGCCGGCCAGATTTCGCCGGGCTACGCCGCCC
CGTGGGCAGCGCCGGGCGTGCCGCTGAAGGCCGTCAAATGGATGTTCCAGAAACACGCGCCGCTCGCCATCCGGCTCGAC
GGCACGCAGTTCCAGCTTCAATGGATGTGGCAGATGTTGCAGAACTGCACATCCGCGCGCTATGCCGTCAACAAGGGCCG
CATGGTGCGACTCGCCGAATACAGCCGCGATTGCCTGCAGGCGCTGCGCGCCGAAACGGGTATTCAGTACGAAGGCCGCA
CGGGCGGCACGCTGCAGCTGTTCCGCACGCAACAGCAACTCGACGGCGCCGCGAAAGACATCGCCGTGCTCGAGGAAGCG
AACGTGCCGTACGAACTGCTGATGCCGGCCGATCTCGCGCGTGCCGAACCGGCGCTCGCCGCGACATCGCACAAACTGAC
GGGCGGCCTGCGCCTGCCTGGCGACGAAACGGGCGATTGCCAGCTGTTCACGACGCGCCTCGCTGCGCTCGCCGAGCAAC
TGGGCGTCAAGTTCCGCTACAACACGCCGATCGACGCACTCGCGATGGAAGGCGGCCGCATCGCCGGCGTGAAGTGCGGC
AATGAAATGGTGCGCGCCGACAACTTCGTTGTCGCGCTGGGCTCGTACTCGACCCAGTTTTTGTCGGGTCTCGTCAAGAT
TCCCGTGTATCCGCTCAAGGGCTACTCGATCACGGCGCCGATCGTCGACGCGAAGTCCGCGCCCGTGTCGACGGTGCTCG
ACGAGACGTACAAGATTGCGATCACGCGTTTCGACGACCGGATTCGTGTCGGCGGGATGGCGGAGATTGTGGGCTTCGAC
AAGAAGCTGAAGCAGGCGCGCCGCGAAACGCTCGAAATGTGCGTCAACGATCTGTTCCCTGGCGGCGGCGATACGTCGAA
AGCGACGTTCTGGACGGGCCTGCGTCCGATGACGCCGGACGGCACGCCGATCGTCGGACGCACGCCCGTGTCGAACCTGT
TCCTGAACACAGGTCATGGCACGCTTGGCTGGACGATGTCGTGCGGCTCGGGTCAACTGCTCGCCGATCTGATCTCGGGC
AAGCGCCCCGCGATCCAGTCGGGCGACCTGTCGGTACATCGCTATCTCGGCGAAACGGCAGGCCAGACGCGTCCGGCTTA
TGCCTGA

Upstream 100 bases:

>100_bases
TAACACTGGGTATCCGGCATTTCACCTCGTCAATCGAACAAATTACGAAGCGCGAGGTTTTCACTACGCCGCGAGCCATT
CGACCTTTTATGGAGCAGCT

Downstream 100 bases:

>100_bases
TTCCGCGATGGGATTTTGTTGACGAAACAACGGCGCCCTCGGGCGCCGTTGTTGTTCGATGCACCGGGGATGTCAGAACT
GGTCTTCGCGCAAGGCCAGC

Product: D-amino acid dehydrogenase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MRVVVLGSGVVGVTSAYYLARAGHEVTVIDREAGPALETSFANAGQISPGYAAPWAAPGVPLKAVKWMFQKHAPLAIRLD
GTQFQLQWMWQMLQNCTSARYAVNKGRMVRLAEYSRDCLQALRAETGIQYEGRTGGTLQLFRTQQQLDGAAKDIAVLEEA
NVPYELLMPADLARAEPALAATSHKLTGGLRLPGDETGDCQLFTTRLAALAEQLGVKFRYNTPIDALAMEGGRIAGVKCG
NEMVRADNFVVALGSYSTQFLSGLVKIPVYPLKGYSITAPIVDAKSAPVSTVLDETYKIAITRFDDRIRVGGMAEIVGFD
KKLKQARRETLEMCVNDLFPGGGDTSKATFWTGLRPMTPDGTPIVGRTPVSNLFLNTGHGTLGWTMSCGSGQLLADLISG
KRPAIQSGDLSVHRYLGETAGQTRPAYA

Sequences:

>Translated_428_residues
MRVVVLGSGVVGVTSAYYLARAGHEVTVIDREAGPALETSFANAGQISPGYAAPWAAPGVPLKAVKWMFQKHAPLAIRLD
GTQFQLQWMWQMLQNCTSARYAVNKGRMVRLAEYSRDCLQALRAETGIQYEGRTGGTLQLFRTQQQLDGAAKDIAVLEEA
NVPYELLMPADLARAEPALAATSHKLTGGLRLPGDETGDCQLFTTRLAALAEQLGVKFRYNTPIDALAMEGGRIAGVKCG
NEMVRADNFVVALGSYSTQFLSGLVKIPVYPLKGYSITAPIVDAKSAPVSTVLDETYKIAITRFDDRIRVGGMAEIVGFD
KKLKQARRETLEMCVNDLFPGGGDTSKATFWTGLRPMTPDGTPIVGRTPVSNLFLNTGHGTLGWTMSCGSGQLLADLISG
KRPAIQSGDLSVHRYLGETAGQTRPAYA
>Mature_428_residues
MRVVVLGSGVVGVTSAYYLARAGHEVTVIDREAGPALETSFANAGQISPGYAAPWAAPGVPLKAVKWMFQKHAPLAIRLD
GTQFQLQWMWQMLQNCTSARYAVNKGRMVRLAEYSRDCLQALRAETGIQYEGRTGGTLQLFRTQQQLDGAAKDIAVLEEA
NVPYELLMPADLARAEPALAATSHKLTGGLRLPGDETGDCQLFTTRLAALAEQLGVKFRYNTPIDALAMEGGRIAGVKCG
NEMVRADNFVVALGSYSTQFLSGLVKIPVYPLKGYSITAPIVDAKSAPVSTVLDETYKIAITRFDDRIRVGGMAEIVGFD
KKLKQARRETLEMCVNDLFPGGGDTSKATFWTGLRPMTPDGTPIVGRTPVSNLFLNTGHGTLGWTMSCGSGQLLADLISG
KRPAIQSGDLSVHRYLGETAGQTRPAYA

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=415, Percent_Identity=74.6987951807229, Blast_Score=654, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 46107; Mature: 46107

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVVLGSGVVGVTSAYYLARAGHEVTVIDREAGPALETSFANAGQISPGYAAPWAAPGV
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCC
PLKAVKWMFQKHAPLAIRLDGTQFQLQWMWQMLQNCTSARYAVNKGRMVRLAEYSRDCLQ
CHHHHHHHHHCCCCEEEEECCCEEHHHHHHHHHHHHHHHHEEECCCCEEEHHHHHHHHHH
ALRAETGIQYEGRTGGTLQLFRTQQQLDGAAKDIAVLEEANVPYELLMPADLARAEPALA
HHHHHCCCEECCCCCCEEEEEECHHHHCCHHHHHHHEECCCCCEEEECCCHHHHCCCCHH
ATSHKLTGGLRLPGDETGDCQLFTTRLAALAEQLGVKFRYNTPIDALAMEGGRIAGVKCG
HCCHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHCCCCCEEEEEECC
NEMVRADNFVVALGSYSTQFLSGLVKIPVYPLKGYSITAPIVDAKSAPVSTVLDETYKIA
CHHEECCCEEEEECCCHHHHHHHHHHCCCEECCCCEEEECEECCCCCCHHHHHCCCEEEE
ITRFDDRIRVGGMAEIVGFDKKLKQARRETLEMCVNDLFPGGGDTSKATFWTGLRPMTPD
EEEECCCEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
GTPIVGRTPVSNLFLNTGHGTLGWTMSCGSGQLLADLISGKRPAIQSGDLSVHRYLGETA
CCEEECCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
GQTRPAYA
CCCCCCCC
>Mature Secondary Structure
MRVVVLGSGVVGVTSAYYLARAGHEVTVIDREAGPALETSFANAGQISPGYAAPWAAPGV
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCC
PLKAVKWMFQKHAPLAIRLDGTQFQLQWMWQMLQNCTSARYAVNKGRMVRLAEYSRDCLQ
CHHHHHHHHHCCCCEEEEECCCEEHHHHHHHHHHHHHHHHEEECCCCEEEHHHHHHHHHH
ALRAETGIQYEGRTGGTLQLFRTQQQLDGAAKDIAVLEEANVPYELLMPADLARAEPALA
HHHHHCCCEECCCCCCEEEEEECHHHHCCHHHHHHHEECCCCCEEEECCCHHHHCCCCHH
ATSHKLTGGLRLPGDETGDCQLFTTRLAALAEQLGVKFRYNTPIDALAMEGGRIAGVKCG
HCCHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHCCCCCEEEEEECC
NEMVRADNFVVALGSYSTQFLSGLVKIPVYPLKGYSITAPIVDAKSAPVSTVLDETYKIA
CHHEECCCEEEEECCCHHHHHHHHHHCCCEECCCCEEEECEECCCCCCHHHHHCCCEEEE
ITRFDDRIRVGGMAEIVGFDKKLKQARRETLEMCVNDLFPGGGDTSKATFWTGLRPMTPD
EEEECCCEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
GTPIVGRTPVSNLFLNTGHGTLGWTMSCGSGQLLADLISGKRPAIQSGDLSVHRYLGETA
CCEEECCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
GQTRPAYA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA