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The map label for this gene is pcl [H]

Identifier: 186475459

GI number: 186475459

Start: 778981

End: 780669

Strand: Direct

Name: pcl [H]

Synonym: Bphy_0694

Alternate gene names: 186475459

Gene position: 778981-780669 (Clockwise)

Preceding gene: 186475458

Following gene: 186475460

Centisome position: 22.39

GC content: 68.09

Gene sequence:

>1689_bases
ATGATCGCGCTGCATGAACTGCTGTCGTGCACGCGCGCCGCAGATGTGCCCGTCTGTCGCGACGACGACTCCGATCGCAC
GATCGATTTCGCCGTCTTCCGTGCGCGCGTCTTCGCAATCGCACGCCAGTTGCGCGAAACGGGCGCGCATCGCTACGCGT
TGCGCATCGACGATCCGTTCGACTTCGCGTGCGCATTATTCGCGCTCTTCGCGTGCGGCAAGACGCCCGTGATTCCGGCG
AACGCGACGCCCGGCTATCTGGCCGATCTCGCCGATGCGTATGACGCCGTGCTCACGGATGCCGACATTCGCGCGCATGC
CGCCGTGCAAGCACCGCCCGCAACGTCGCTGCACATCGATCCGAACGCGCCGCTCACGCTCTACACGTCGGGCAGCAGCG
GCACGCCCAAGCCGATCCACAAGACGCTCGCGCAGTTCGACGCCGAAGTGCGTACACTCGAACGCGAGTGGGGCCAACTC
GTCGGCGACGCGACGATGCTCGCGAGCGTGCCGCATCATCACATCTACGGCCTGCTGTTTCGCGTGATGTGGCCGCTCGC
GGCCGGGCGCGCGTTCGACCGGGCTGTCTGCATCGAGCCACAGCATGTGCAGGCGCGCATCGGCCAATGCGGCGCGACGG
TGGTTGTGTCGTCGCCCGCGCAGCTGTCGCGCTGGCCAGCGTTGCCCGGCTTCGCGGCGCTCGCGCCCGTGCCGCGCGCT
TTCTTTTCGTCGGGTGGTCCGCTTTCGGCGGAAGCCGCCGCGGAATACGCCGCCGCCTTCGGCGCCGCGCCGATCGAGAT
TTACGGCAGCACCGAAACGGGCGGCATCGCGTGGCGACGTCAGAACGAGACGCCGGCGTGGCGTCCCGTCAGCGGCGTCG
ACGTGCGGCGCGGCGAGGATGGCGCGTTGAACGTGCGCTCGGCGCATCTCGGTCACGACGACTGGCATCGCACCGACGAC
GCCATTGCGTTCGACGACGAAGGCCGCTTCCGTCTGTTGGGACGTCTGGATCGCGTGATCAAGCTCGACGGCAAGCGTGT
GTCGCTGCCCGAGGTCGAAGCGCGTCTCGCGCTGCATCCGTATGTCGCGCAAGCGGCCGTCGTGAAGCTCGCGGGCGCAT
CGCGCGAACGCGTGGGCGCCGTGGTCGCGCTCAGCGACGCGGGCGGCGACGCGCTGCGCAGCGAAGGGCGCGTCGCACTG
GCGAAGACGCTGCGCCGCCACCTCGCCGCATATTTCGATGTGGTCGTGCTGCCGCGTCATTGGCGCTTTCGCGTCGCGTT
GCCGTTCGATGCGCGCGGCAAGCTGCCCGTCGCGGCCGTGGCGGCCGCATTCGAGCCGCGCAGCGAAGGCTTCGAAGTGC
TGTCCGAAGCACGCCATGGCGAGCACCTGCATTACGAACTGCGCGTGCCCCCCGCGCTCGTGCATTTCGAAGGCCACTTC
CCCGGTTTGCCGATCCTGCCCGGCGTCGTGCAACTCGACTGGGCGATCCGCCTCGCCGCCGAGCATGTGACGGGTGTGCG
TGAAATCGAATCCGTCGATCGCCTGAAATTCACCGCGCCTGTGATGCCGGGCGCCGTGCTCGATCTCAAGCTCTCGCACG
ATGCCGCGCGCAGGCGCGTGCAATTCGCGTATCGCGTCGATGCACGCGACAGTTCGTCGGGCGTGATCGTGTATCGGGAG
CACACATGA

Upstream 100 bases:

>100_bases
ACGGCGCGATTGCATATGGGCTTATCGGCGTGCTGCTGGCGGGCGAAATCGCATGGCGCTATCTCGTGATCCTGCCACGT
GCCCGGCGCTCGGAGGCGGC

Downstream 100 bases:

>100_bases
GCGAAGCCAAGGCGATTTTCGCGCCGTGCGTCGTCATTCCGATCTACAACCATAAGGATGCGATCGGCGGCACGGTCGAG
CGTCTTCTCGTGCACGCGTT

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: 4CL; 4-coumaroyl-CoA synthase [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MIALHELLSCTRAADVPVCRDDDSDRTIDFAVFRARVFAIARQLRETGAHRYALRIDDPFDFACALFALFACGKTPVIPA
NATPGYLADLADAYDAVLTDADIRAHAAVQAPPATSLHIDPNAPLTLYTSGSSGTPKPIHKTLAQFDAEVRTLEREWGQL
VGDATMLASVPHHHIYGLLFRVMWPLAAGRAFDRAVCIEPQHVQARIGQCGATVVVSSPAQLSRWPALPGFAALAPVPRA
FFSSGGPLSAEAAAEYAAAFGAAPIEIYGSTETGGIAWRRQNETPAWRPVSGVDVRRGEDGALNVRSAHLGHDDWHRTDD
AIAFDDEGRFRLLGRLDRVIKLDGKRVSLPEVEARLALHPYVAQAAVVKLAGASRERVGAVVALSDAGGDALRSEGRVAL
AKTLRRHLAAYFDVVVLPRHWRFRVALPFDARGKLPVAAVAAAFEPRSEGFEVLSEARHGEHLHYELRVPPALVHFEGHF
PGLPILPGVVQLDWAIRLAAEHVTGVREIESVDRLKFTAPVMPGAVLDLKLSHDAARRRVQFAYRVDARDSSSGVIVYRE
HT

Sequences:

>Translated_562_residues
MIALHELLSCTRAADVPVCRDDDSDRTIDFAVFRARVFAIARQLRETGAHRYALRIDDPFDFACALFALFACGKTPVIPA
NATPGYLADLADAYDAVLTDADIRAHAAVQAPPATSLHIDPNAPLTLYTSGSSGTPKPIHKTLAQFDAEVRTLEREWGQL
VGDATMLASVPHHHIYGLLFRVMWPLAAGRAFDRAVCIEPQHVQARIGQCGATVVVSSPAQLSRWPALPGFAALAPVPRA
FFSSGGPLSAEAAAEYAAAFGAAPIEIYGSTETGGIAWRRQNETPAWRPVSGVDVRRGEDGALNVRSAHLGHDDWHRTDD
AIAFDDEGRFRLLGRLDRVIKLDGKRVSLPEVEARLALHPYVAQAAVVKLAGASRERVGAVVALSDAGGDALRSEGRVAL
AKTLRRHLAAYFDVVVLPRHWRFRVALPFDARGKLPVAAVAAAFEPRSEGFEVLSEARHGEHLHYELRVPPALVHFEGHF
PGLPILPGVVQLDWAIRLAAEHVTGVREIESVDRLKFTAPVMPGAVLDLKLSHDAARRRVQFAYRVDARDSSSGVIVYRE
HT
>Mature_562_residues
MIALHELLSCTRAADVPVCRDDDSDRTIDFAVFRARVFAIARQLRETGAHRYALRIDDPFDFACALFALFACGKTPVIPA
NATPGYLADLADAYDAVLTDADIRAHAAVQAPPATSLHIDPNAPLTLYTSGSSGTPKPIHKTLAQFDAEVRTLEREWGQL
VGDATMLASVPHHHIYGLLFRVMWPLAAGRAFDRAVCIEPQHVQARIGQCGATVVVSSPAQLSRWPALPGFAALAPVPRA
FFSSGGPLSAEAAAEYAAAFGAAPIEIYGSTETGGIAWRRQNETPAWRPVSGVDVRRGEDGALNVRSAHLGHDDWHRTDD
AIAFDDEGRFRLLGRLDRVIKLDGKRVSLPEVEARLALHPYVAQAAVVKLAGASRERVGAVVALSDAGGDALRSEGRVAL
AKTLRRHLAAYFDVVVLPRHWRFRVALPFDARGKLPVAAVAAAFEPRSEGFEVLSEARHGEHLHYELRVPPALVHFEGHF
PGLPILPGVVQLDWAIRLAAEHVTGVREIESVDRLKFTAPVMPGAVLDLKLSHDAARRRVQFAYRVDARDSSSGVIVYRE
HT

Specific function: Converts p-coumaric acid into p-coumaryl CoA. This is necessary for the activation of the photoactive yellow protein (PYP) chromophore [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Caenorhabditis elegans, GI32563687, Length=398, Percent_Identity=25.1256281407035, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17558820, Length=317, Percent_Identity=25.8675078864353, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012743
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.12 [H]

Molecular weight: Translated: 61109; Mature: 61109

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALHELLSCTRAADVPVCRDDDSDRTIDFAVFRARVFAIARQLRETGAHRYALRIDDPF
CCCHHHHHHHHHHCCCCEECCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCH
DFACALFALFACGKTPVIPANATPGYLADLADAYDAVLTDADIRAHAAVQAPPATSLHID
HHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEC
PNAPLTLYTSGSSGTPKPIHKTLAQFDAEVRTLEREWGQLVGDATMLASVPHHHIYGLLF
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHH
RVMWPLAAGRAFDRAVCIEPQHVQARIGQCGATVVVSSPAQLSRWPALPGFAALAPVPRA
HHHHHHHCCCCCCCEEECCCHHHHHHHCCCCCEEEEECCHHHHHCCCCCCHHHHCCCCHH
FFSSGGPLSAEAAAEYAAAFGAAPIEIYGSTETGGIAWRRQNETPAWRPVSGVDVRRGED
HHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCEEECCCC
GALNVRSAHLGHDDWHRTDDAIAFDDEGRFRLLGRLDRVIKLDGKRVSLPEVEARLALHP
CCEEEEEECCCCCHHCCCCCCEEECCCCCCHHHHHHHHHEEECCCEECCCHHHHHHHHCH
YVAQAAVVKLAGASRERVGAVVALSDAGGDALRSEGRVALAKTLRRHLAAYFDVVVLPRH
HHHHHHHHHHCCCCCHHCEEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHEEEECCC
WRFRVALPFDARGKLPVAAVAAAFEPRSEGFEVLSEARHGEHLHYELRVPPALVHFEGHF
CEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCC
PGLPILPGVVQLDWAIRLAAEHVTGVREIESVDRLKFTAPVMPGAVLDLKLSHDAARRRV
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEECCHHHHHHE
QFAYRVDARDSSSGVIVYREHT
EEEEEECCCCCCCCEEEEECCC
>Mature Secondary Structure
MIALHELLSCTRAADVPVCRDDDSDRTIDFAVFRARVFAIARQLRETGAHRYALRIDDPF
CCCHHHHHHHHHHCCCCEECCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCH
DFACALFALFACGKTPVIPANATPGYLADLADAYDAVLTDADIRAHAAVQAPPATSLHID
HHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEC
PNAPLTLYTSGSSGTPKPIHKTLAQFDAEVRTLEREWGQLVGDATMLASVPHHHIYGLLF
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHH
RVMWPLAAGRAFDRAVCIEPQHVQARIGQCGATVVVSSPAQLSRWPALPGFAALAPVPRA
HHHHHHHCCCCCCCEEECCCHHHHHHHCCCCCEEEEECCHHHHHCCCCCCHHHHCCCCHH
FFSSGGPLSAEAAAEYAAAFGAAPIEIYGSTETGGIAWRRQNETPAWRPVSGVDVRRGED
HHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCEEECCCC
GALNVRSAHLGHDDWHRTDDAIAFDDEGRFRLLGRLDRVIKLDGKRVSLPEVEARLALHP
CCEEEEEECCCCCHHCCCCCCEEECCCCCCHHHHHHHHHEEECCCEECCCHHHHHHHHCH
YVAQAAVVKLAGASRERVGAVVALSDAGGDALRSEGRVALAKTLRRHLAAYFDVVVLPRH
HHHHHHHHHHCCCCCHHCEEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHEEEECCC
WRFRVALPFDARGKLPVAAVAAAFEPRSEGFEVLSEARHGEHLHYELRVPPALVHFEGHF
CEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCC
PGLPILPGVVQLDWAIRLAAEHVTGVREIESVDRLKFTAPVMPGAVLDLKLSHDAARRRV
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEECCHHHHHHE
QFAYRVDARDSSSGVIVYREHT
EEEEEECCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8670821; 7981196 [H]