Click here to switch to the map view.

The map label for this gene is nodW [H]

Identifier: 186475412

GI number: 186475412

Start: 729061

End: 729447

Strand: Direct

Name: nodW [H]

Synonym: Bphy_0646

Alternate gene names: 186475412

Gene position: 729061-729447 (Clockwise)

Preceding gene: 186475411

Following gene: 186475413

Centisome position: 20.95

GC content: 64.6

Gene sequence:

>387_bases
ATGGGCACAAACAAACCATTCGTCGTCGTGGTGGACGACGATGCGTCGGTGGGCAGGGCAATCAGGCGTCTGTTGCGCTC
AGTCGGGATTGCAGCCGATACGTACACGAGTGGCGACGAGTTTCTCGACGTGCTGTCCGCCACACCCTCGTATCGTCCCG
ACTGCGTCATCCTCGACGTGCAGATGCCAGGCTCGAACGGCATCGAGGTGCAGCGCCGGCTGGCGGGGGGCGCTGTGCCC
GTCATTTTCATCACTGCCCACGACGACGCGGGCGTGCGCGAAACCGCGCTTGCTGCGGGCGCGCGTGCCTACCTGCGCAA
GCCCTTCAACGACGTGCTGTTCATCAGGACGGTGTGTGCCGTGCTCGGCATCGCTGCGCCGCTATGA

Upstream 100 bases:

>100_bases
ACCAAGGTCCAATACCACCGACGCCCGCAGTCTCATAGTCTATTGCTGAGACGCCCACGCATCGTCCGCGTGGACGTGGC
GGCTCAACGGGCTAATCGGA

Downstream 100 bases:

>100_bases
GCACGTTGCCGTTACGCTCGAACGGTCCCGGCCCCCGGCGTGCACGCATTGATGCGCGAGACCCTTGTCGCTGGACGCAG
TATTGGACCAAGGTCCAATT

Product: response regulator receiver protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MGTNKPFVVVVDDDASVGRAIRRLLRSVGIAADTYTSGDEFLDVLSATPSYRPDCVILDVQMPGSNGIEVQRRLAGGAVP
VIFITAHDDAGVRETALAAGARAYLRKPFNDVLFIRTVCAVLGIAAPL

Sequences:

>Translated_128_residues
MGTNKPFVVVVDDDASVGRAIRRLLRSVGIAADTYTSGDEFLDVLSATPSYRPDCVILDVQMPGSNGIEVQRRLAGGAVP
VIFITAHDDAGVRETALAAGARAYLRKPFNDVLFIRTVCAVLGIAAPL
>Mature_127_residues
GTNKPFVVVVDDDASVGRAIRRLLRSVGIAADTYTSGDEFLDVLSATPSYRPDCVILDVQMPGSNGIEVQRRLAGGAVPV
IFITAHDDAGVRETALAAGARAYLRKPFNDVLFIRTVCAVLGIAAPL

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=105, Percent_Identity=37.1428571428571, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI1789402, Length=104, Percent_Identity=34.6153846153846, Blast_Score=60, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13558; Mature: 13427

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTNKPFVVVVDDDASVGRAIRRLLRSVGIAADTYTSGDEFLDVLSATPSYRPDCVILDV
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEE
QMPGSNGIEVQRRLAGGAVPVIFITAHDDAGVRETALAAGARAYLRKPFNDVLFIRTVCA
ECCCCCCHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VLGIAAPL
HHHHCCCC
>Mature Secondary Structure 
GTNKPFVVVVDDDASVGRAIRRLLRSVGIAADTYTSGDEFLDVLSATPSYRPDCVILDV
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEE
QMPGSNGIEVQRRLAGGAVPVIFITAHDDAGVRETALAAGARAYLRKPFNDVLFIRTVCA
ECCCCCCHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VLGIAAPL
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]