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The map label for this gene is fixL [H]

Identifier: 186475410

GI number: 186475410

Start: 726816

End: 728279

Strand: Direct

Name: fixL [H]

Synonym: Bphy_0644

Alternate gene names: 186475410

Gene position: 726816-728279 (Clockwise)

Preceding gene: 186475409

Following gene: 186475411

Centisome position: 20.89

GC content: 64.62

Gene sequence:

>1464_bases
ATGGCAGAAGCTCCGCGCGCCCGGTTCGGGCAGCCGGCGCCCTGGTCAGTTGCTAACAACAACGAAGGCGTGCGCACGTG
GTCGCAGCTGGTCGGGTGCGAACGTCTTGAGGAACGGTACGTCATGCACATCGCTATTTCTGCTCTGACAGTCGCGGTGG
CTGCAATCGCTGTCTGGTGGTCGCTGCATCCGCGCCGCGTCGCGCCCGAACGGGCCGCAGGTTCCATGTCCACTCGCTAC
GGCGTCTCCGGGTTACCTGTGCTGTTGCGCATCACCAATCCGCTTGCGCGTGTGCGAAGCGCGCAAAGCGCAGCCGGTTC
CTTCGCCTTTTCGCGCCGCCAGCCCGGCACGATGAACGAGCTGGCCGACGTATTCGAATGCTTGCCCGTCGCATCGGTCG
TGTTCGACGAACATGGCGTGATAGTCCTCGCCAATGCGCAGGCCGAACGGCTATTCGACTACCGGCGCGGCGTGCTGGCC
GGCGCATGTGTCGGCATGGTTGCACCGGCGCTTTCGCAGCAAAACAGCGCGACTGGCGGCACCGCGGAGAGCGCGGGCCG
TGCCGGGTCGACACGCACGCACAGCTTGCGCGCACGGCGCCGCGACGGCAGCGAGTTTCCCGTCGAAATCGCAACCAGCG
CGATCCGCTATGAAGGGCGCATTGCGACTGTCGCGTTCATCACCGATGTGACAGAGCGCTTCGAACTCGAACGCAATCGT
CGGGAACTGGCGCATCTGACGCGCGTATCGACGATGGGCGAACTCGCCGCATCGCTCGCGCACGAACTGAACCAGCCGCT
GACGGCGATTCTCAGCAACGGCCAGGCCGCACAGCGCTTCATGGATCAGGAGCCGAGCGATCTTGCCGAGGTCCGCGACA
TCCTGAAGGACATCGTCGACGACAGCGGTCGCGCAAGCGAAGTGATCCGACGGATGCGTGCATTCGTGAAGAAGGACGAG
CAGCAGCAACTCGTCAGTCTGGACGCAGGCGGCTTGTTGCGCGACGTCGCGCTGCTCGTGCACGGCGATGCCGTCGCGCG
CGGCATCCATGTGTCTCAGGTGATCGACGACGGCTTGCCGCACGTGCGTGGCGACAAGGTGCAACTGCAGCAGGTGTTAC
TCAATCTGCTGCTCAACGCATTCGATGCTGTGAGCGAGTGCGGATCGCGCGATCGCGTGGTCGCGCTTTTTGCGCAACCC
GACCGCGAAGGCATGGTGCGCATCGCCGTGCGTGATCGCGGACATGGGCTGACGTCCGACAGGCTCGAATGCATCTTCAA
GCCATTCGTCACATCGAAGGCGCAAGGCCTGGGGCTCGGGCTGTCGATCAGCCGTTCGATCGTGCAGATGCACGGCGGCC
GCCTTTGGGCAGAGAACAACGCGGATCAGGGCATGACGTTCTATGTCGCGCTGCCGGCAGCGCGTGAAGCAGGCAGCGCG
TGCGCTGCGCAGGCGGCGTCATGA

Upstream 100 bases:

>100_bases
GGTCACTACTGAAAGGTTCATTTTTTTCTGGCGCAGTGATTGACGTTGTCGAGCAACCAGTGCATCAGTAGTACAAGACG
TTGTCACGGATTCATGCGTC

Downstream 100 bases:

>100_bases
ACCCGTCTGCGCCGCTCGTCTACGTCGTCGACGACGACCCGTCGGTGCGCCGCGCGCTCACGCGGCTTATCAGGTCGGCG
GGCCACGAGGTCGAGGCTTA

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MAEAPRARFGQPAPWSVANNNEGVRTWSQLVGCERLEERYVMHIAISALTVAVAAIAVWWSLHPRRVAPERAAGSMSTRY
GVSGLPVLLRITNPLARVRSAQSAAGSFAFSRRQPGTMNELADVFECLPVASVVFDEHGVIVLANAQAERLFDYRRGVLA
GACVGMVAPALSQQNSATGGTAESAGRAGSTRTHSLRARRRDGSEFPVEIATSAIRYEGRIATVAFITDVTERFELERNR
RELAHLTRVSTMGELAASLAHELNQPLTAILSNGQAAQRFMDQEPSDLAEVRDILKDIVDDSGRASEVIRRMRAFVKKDE
QQQLVSLDAGGLLRDVALLVHGDAVARGIHVSQVIDDGLPHVRGDKVQLQQVLLNLLLNAFDAVSECGSRDRVVALFAQP
DREGMVRIAVRDRGHGLTSDRLECIFKPFVTSKAQGLGLGLSISRSIVQMHGGRLWAENNADQGMTFYVALPAAREAGSA
CAAQAAS

Sequences:

>Translated_487_residues
MAEAPRARFGQPAPWSVANNNEGVRTWSQLVGCERLEERYVMHIAISALTVAVAAIAVWWSLHPRRVAPERAAGSMSTRY
GVSGLPVLLRITNPLARVRSAQSAAGSFAFSRRQPGTMNELADVFECLPVASVVFDEHGVIVLANAQAERLFDYRRGVLA
GACVGMVAPALSQQNSATGGTAESAGRAGSTRTHSLRARRRDGSEFPVEIATSAIRYEGRIATVAFITDVTERFELERNR
RELAHLTRVSTMGELAASLAHELNQPLTAILSNGQAAQRFMDQEPSDLAEVRDILKDIVDDSGRASEVIRRMRAFVKKDE
QQQLVSLDAGGLLRDVALLVHGDAVARGIHVSQVIDDGLPHVRGDKVQLQQVLLNLLLNAFDAVSECGSRDRVVALFAQP
DREGMVRIAVRDRGHGLTSDRLECIFKPFVTSKAQGLGLGLSISRSIVQMHGGRLWAENNADQGMTFYVALPAAREAGSA
CAAQAAS
>Mature_486_residues
AEAPRARFGQPAPWSVANNNEGVRTWSQLVGCERLEERYVMHIAISALTVAVAAIAVWWSLHPRRVAPERAAGSMSTRYG
VSGLPVLLRITNPLARVRSAQSAAGSFAFSRRQPGTMNELADVFECLPVASVVFDEHGVIVLANAQAERLFDYRRGVLAG
ACVGMVAPALSQQNSATGGTAESAGRAGSTRTHSLRARRRDGSEFPVEIATSAIRYEGRIATVAFITDVTERFELERNRR
ELAHLTRVSTMGELAASLAHELNQPLTAILSNGQAAQRFMDQEPSDLAEVRDILKDIVDDSGRASEVIRRMRAFVKKDEQ
QQLVSLDAGGLLRDVALLVHGDAVARGIHVSQVIDDGLPHVRGDKVQLQQVLLNLLLNAFDAVSECGSRDRVVALFAQPD
REGMVRIAVRDRGHGLTSDRLECIFKPFVTSKAQGLGLGLSISRSIVQMHGGRLWAENNADQGMTFYVALPAAREAGSAC
AAQAAS

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=32.9004329004329, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1788549, Length=379, Percent_Identity=27.9683377308707, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI87081816, Length=233, Percent_Identity=30.4721030042918, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=284, Percent_Identity=24.6478873239437, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52689; Mature: 52557

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAPRARFGQPAPWSVANNNEGVRTWSQLVGCERLEERYVMHIAISALTVAVAAIAVWW
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLHPRRVAPERAAGSMSTRYGVSGLPVLLRITNPLARVRSAQSAAGSFAFSRRQPGTMNE
HCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCHHCCCCCCCCHHH
LADVFECLPVASVVFDEHGVIVLANAQAERLFDYRRGVLAGACVGMVAPALSQQNSATGG
HHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TAESAGRAGSTRTHSLRARRRDGSEFPVEIATSAIRYEGRIATVAFITDVTERFELERNR
CCHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHH
RELAHLTRVSTMGELAASLAHELNQPLTAILSNGQAAQRFMDQEPSDLAEVRDILKDIVD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHC
DSGRASEVIRRMRAFVKKDEQQQLVSLDAGGLLRDVALLVHGDAVARGIHVSQVIDDGLP
CCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHCCHHHHHHHCCCC
HVRGDKVQLQQVLLNLLLNAFDAVSECGSRDRVVALFAQPDREGMVRIAVRDRGHGLTSD
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHH
RLECIFKPFVTSKAQGLGLGLSISRSIVQMHGGRLWAENNADQGMTFYVALPAAREAGSA
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCHHHCCCH
CAAQAAS
HHHCCCC
>Mature Secondary Structure 
AEAPRARFGQPAPWSVANNNEGVRTWSQLVGCERLEERYVMHIAISALTVAVAAIAVWW
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLHPRRVAPERAAGSMSTRYGVSGLPVLLRITNPLARVRSAQSAAGSFAFSRRQPGTMNE
HCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCHHCCCCCCCCHHH
LADVFECLPVASVVFDEHGVIVLANAQAERLFDYRRGVLAGACVGMVAPALSQQNSATGG
HHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TAESAGRAGSTRTHSLRARRRDGSEFPVEIATSAIRYEGRIATVAFITDVTERFELERNR
CCHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHH
RELAHLTRVSTMGELAASLAHELNQPLTAILSNGQAAQRFMDQEPSDLAEVRDILKDIVD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHC
DSGRASEVIRRMRAFVKKDEQQQLVSLDAGGLLRDVALLVHGDAVARGIHVSQVIDDGLP
CCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHCCHHHHHHHCCCC
HVRGDKVQLQQVLLNLLLNAFDAVSECGSRDRVVALFAQPDREGMVRIAVRDRGHGLTSD
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHH
RLECIFKPFVTSKAQGLGLGLSISRSIVQMHGGRLWAENNADQGMTFYVALPAAREAGSA
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCHHHCCCH
CAAQAAS
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]