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The map label for this gene is bcr [H]

Identifier: 186475390

GI number: 186475390

Start: 700482

End: 701690

Strand: Direct

Name: bcr [H]

Synonym: Bphy_0624

Alternate gene names: 186475390

Gene position: 700482-701690 (Clockwise)

Preceding gene: 186475381

Following gene: 186475394

Centisome position: 20.13

GC content: 62.61

Gene sequence:

>1209_bases
ATGTCTCACGACGTCAGATCCCGGCCCGACGCGCGGCTGATTCTGCTGCTCGGCGCGCTCGCCGCGTGCGGGCCGATTTC
GATCGATATGTATCTGCCCAGCCTGCCGTCGATCACGCGAGCGTTCAGCGTCAGTGCGGGCGCCGCGCAGAGCACGCTGA
CGAGCTTCATGCTCGGCTTCTCGATCGGCATGCTGCTGTATGGGCCGATGTCGGACGCATATGGGCGGCGTCCCGTGCTG
CTCGGCGGCATTGTCATGTACACGCTCGCGACGATCGTTTGTGCGTTGTCGCCGTCGATCGGTTCGCTCATCACGTTCCG
CTTCGTGCAGGCGCTCGGCGCGGGTGCTGCGTCGGTGCTGGCGCGTGCGATCGCCCGCGACGCGCATGGTCCTGCCGATG
CAGCGCGTGTGCTGTCGATGCTTGCCATCGTCACGTCGATCGGACCGTTGCTTGCGCCGTTGATCGGCGGGCAGTTGCTG
CTGCTGGGCGGCTGGCGGGTCGTCTTCATCGCGCTGACGATTTTCGGTGCGATATGCGCCGCGACTGCATTCATGCGGGT
GCCGGAGACGTGGCCGCGGGAGAAGCGCGCACACGGTGCGGTGTTTCAGTCATTTGCCGCCTACGGCACGCTGCTGAAAG
ACCCTGTGACCTGGGGGCACATGCTGTGCGGCGGCATGGCGTTCGCTTCCATGTTTGCCTATATCACGGCGACGCCGTTC
GTTTATATCGAGTACTTCCATGTGTCTGCGCAGCACTATGGCTTTCTGTTCGGAATGAACATCGTCGGTATCATGCTCGG
CAACTTCATCAACACACGGGTTGTCGGCCGCACGGGGCCGCTGCCCGTGATTTCCGTTGCTGCGTCGATCAGCTTCGTCG
CTTCGTTGTTCGTCGCATTGATGTGTCTGACGGGGTGGGGCGGGTTGTGGTCGATCGTGGCCGGCTTGTTTTTCGTGGTC
GGTGTGGTTGGCTTGCTGTCGGCCAATTGCACTACCGATCTGATGCACAGATATCCACGTAATGCCGGCGCGGCCGCGGC
TGTGTTCGGGGCTGTGCAGTTGGCGTTAGGGGCTTTGTCTTCCTTGGCTGTCGGGCTTTGGCATGATGGATCGCCGCAAG
GCATGGGGGTGACTATAGCGGTCGCCGGCTCTTTGTGCTTTGTTGGCAGGTTTCTGGTAGTGCGGTGGCATGGGAGGCCG
GCGTTGTAG

Upstream 100 bases:

>100_bases
TATGGGGCGTCGGTTACAATCGGGGGCCGTATCCGCAGCGCTGCACGGACCGCCGGTCCAGCATCGCCGTTCCTTGCGCG
CTGCTTCTCACTGGAATTCC

Downstream 100 bases:

>100_bases
TTGTTGTTGTCTGCGACGCCGTCGCCGTTCTCGCCTTTTCGCTGGCATCCGCGTGATGTTCTGGTTTGCCGGCGCTGCCA
GCAAATCAAACATGCCACGC

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSHDVRSRPDARLILLLGALAACGPISIDMYLPSLPSITRAFSVSAGAAQSTLTSFMLGFSIGMLLYGPMSDAYGRRPVL
LGGIVMYTLATIVCALSPSIGSLITFRFVQALGAGAASVLARAIARDAHGPADAARVLSMLAIVTSIGPLLAPLIGGQLL
LLGGWRVVFIALTIFGAICAATAFMRVPETWPREKRAHGAVFQSFAAYGTLLKDPVTWGHMLCGGMAFASMFAYITATPF
VYIEYFHVSAQHYGFLFGMNIVGIMLGNFINTRVVGRTGPLPVISVAASISFVASLFVALMCLTGWGGLWSIVAGLFFVV
GVVGLLSANCTTDLMHRYPRNAGAAAAVFGAVQLALGALSSLAVGLWHDGSPQGMGVTIAVAGSLCFVGRFLVVRWHGRP
AL

Sequences:

>Translated_402_residues
MSHDVRSRPDARLILLLGALAACGPISIDMYLPSLPSITRAFSVSAGAAQSTLTSFMLGFSIGMLLYGPMSDAYGRRPVL
LGGIVMYTLATIVCALSPSIGSLITFRFVQALGAGAASVLARAIARDAHGPADAARVLSMLAIVTSIGPLLAPLIGGQLL
LLGGWRVVFIALTIFGAICAATAFMRVPETWPREKRAHGAVFQSFAAYGTLLKDPVTWGHMLCGGMAFASMFAYITATPF
VYIEYFHVSAQHYGFLFGMNIVGIMLGNFINTRVVGRTGPLPVISVAASISFVASLFVALMCLTGWGGLWSIVAGLFFVV
GVVGLLSANCTTDLMHRYPRNAGAAAAVFGAVQLALGALSSLAVGLWHDGSPQGMGVTIAVAGSLCFVGRFLVVRWHGRP
AL
>Mature_401_residues
SHDVRSRPDARLILLLGALAACGPISIDMYLPSLPSITRAFSVSAGAAQSTLTSFMLGFSIGMLLYGPMSDAYGRRPVLL
GGIVMYTLATIVCALSPSIGSLITFRFVQALGAGAASVLARAIARDAHGPADAARVLSMLAIVTSIGPLLAPLIGGQLLL
LGGWRVVFIALTIFGAICAATAFMRVPETWPREKRAHGAVFQSFAAYGTLLKDPVTWGHMLCGGMAFASMFAYITATPFV
YIEYFHVSAQHYGFLFGMNIVGIMLGNFINTRVVGRTGPLPVISVAASISFVASLFVALMCLTGWGGLWSIVAGLFFVVG
VVGLLSANCTTDLMHRYPRNAGAAAAVFGAVQLALGALSSLAVGLWHDGSPQGMGVTIAVAGSLCFVGRFLVVRWHGRPA
L

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=384, Percent_Identity=34.6354166666667, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI48994889, Length=237, Percent_Identity=31.2236286919831, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI87082312, Length=359, Percent_Identity=29.8050139275766, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790146, Length=182, Percent_Identity=32.4175824175824, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787065, Length=262, Percent_Identity=26.3358778625954, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790794, Length=361, Percent_Identity=22.1606648199446, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790195, Length=137, Percent_Identity=29.1970802919708, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319657, Length=168, Percent_Identity=27.9761904761905, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324264, Length=161, Percent_Identity=27.9503105590062, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325414, Length=123, Percent_Identity=28.4552845528455, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42108; Mature: 41977

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDVRSRPDARLILLLGALAACGPISIDMYLPSLPSITRAFSVSAGAAQSTLTSFMLGF
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHH
SIGMLLYGPMSDAYGRRPVLLGGIVMYTLATIVCALSPSIGSLITFRFVQALGAGAASVL
HHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHH
ARAIARDAHGPADAARVLSMLAIVTSIGPLLAPLIGGQLLLLGGWRVVFIALTIFGAICA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHH
ATAFMRVPETWPREKRAHGAVFQSFAAYGTLLKDPVTWGHMLCGGMAFASMFAYITATPF
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCE
VYIEYFHVSAQHYGFLFGMNIVGIMLGNFINTRVVGRTGPLPVISVAASISFVASLFVAL
EEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHH
MCLTGWGGLWSIVAGLFFVVGVVGLLSANCTTDLMHRYPRNAGAAAAVFGAVQLALGALS
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SLAVGLWHDGSPQGMGVTIAVAGSLCFVGRFLVVRWHGRPAL
HHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure 
SHDVRSRPDARLILLLGALAACGPISIDMYLPSLPSITRAFSVSAGAAQSTLTSFMLGF
CCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHH
SIGMLLYGPMSDAYGRRPVLLGGIVMYTLATIVCALSPSIGSLITFRFVQALGAGAASVL
HHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHH
ARAIARDAHGPADAARVLSMLAIVTSIGPLLAPLIGGQLLLLGGWRVVFIALTIFGAICA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHH
ATAFMRVPETWPREKRAHGAVFQSFAAYGTLLKDPVTWGHMLCGGMAFASMFAYITATPF
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCE
VYIEYFHVSAQHYGFLFGMNIVGIMLGNFINTRVVGRTGPLPVISVAASISFVASLFVAL
EEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHH
MCLTGWGGLWSIVAGLFFVVGVVGLLSANCTTDLMHRYPRNAGAAAAVFGAVQLALGALS
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SLAVGLWHDGSPQGMGVTIAVAGSLCFVGRFLVVRWHGRPAL
HHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]