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The map label for this gene is braE [H]

Identifier: 186475357

GI number: 186475357

Start: 668602

End: 669771

Strand: Direct

Name: braE [H]

Synonym: Bphy_0590

Alternate gene names: 186475357

Gene position: 668602-669771 (Clockwise)

Preceding gene: 186475356

Following gene: 186475358

Centisome position: 19.22

GC content: 63.08

Gene sequence:

>1170_bases
ATGACCTCAATTCAACCGATCGAGCCGTCCACGACGCTCATCCCCGAACGCAACCTCACGAAGACGCTGACGGTGGGCAT
CATCACCGCCGTGTTCGTGATCGCTGCACCGATGATCATCGGTGCAGCGGGCGGCAACTACTGGGTCCGCGTGCTCGACT
TCGCGATGCTGTACGTGATGCTCGCGCTCGGTCTGAACGTGGTGGTCGGCTTCGCCGGCCTGCTGGACCTGGGCTACATC
GCGTTCTACGCAGTGGGCGCCTACACGGCAGCGTTGCTTAGCTCGCCGCACTTGTCGTCGCAGTTCGAATGGATCGCGCA
TTTGGCGCCGGCCGGCCTGCATGTGCCGTTCCTCATCATCATCCCGTGCGCGATGGCGATTGCGGCGATCTGCGGCGTGA
TTCTTGGCGCACCGACGCTGCGTCTGCGCGGCGACTACCTCGCAATCGTGACGTTGGGCTTCGGGGAGATCGTCCGGATC
TTCCTGAACAACCTCGACCGTCCGGTGAACATCACGAACGGCCCGAAGGGCATCACGGGTATCGATCCGATCCACGTCGG
CGACTTCAGCCTTGCGCAGACGCACTCGCTGTTCGGCCTGACGTTCCCGTCGGTGTACTCGTACTACTACGTGTTCGTGC
TGTGCGCGCTCTTCGTAATCTGGGTGTGTACGCGTCTGCAGCACTCGCGCATCGGCCGCGCGTGGGCTGCGATCCGCGAA
GACGAAATCGCCGCGAAGGCGATGGGCATCAATACGCGCGACGTGAAGCTGCTGGCGTTCGCGATGGGCGCATCGTTCGG
CGGCCTGTCGGGCGCGATGTTCGGCGCGTTCCAGGGCTTCGTTTCGCCGGAATCGTTCACGCTGCCGGAATCGATCGTCG
TGCTGGCTTGCGTGGTGCTGGGCGGCATGGGCCACATCCCGGGCGTGATTCTCGGCGCGGTGCTGCTCGCCGTGCTGCCG
GAATTCCTGCGCTCGACGATGGGTCCGCTGCAGCACATGATCTTCGGTCATGAAATCGTCGATACGGAAGTGATCCGTCA
GCTCGTGTACGCACTCGCGATGGTGCTGATCATGCTGTACCGCTCGGAAGGCCTGTGGCCGTCACCGAAGCACGAAGACA
AGATCGCGAAGCTTGCGAAGCGCAACGGCAAGAAGCCGGTGCGTGCTTGA

Upstream 100 bases:

>100_bases
CATCGTGCTGATCATCGTGCTGGTGTTCCGTCCGTCGGGCCTGCTTGGCGAACGTGTCGCGGATCGCGCTTAACGTGCGC
TTGGGAAAGGAGCAAATACA

Downstream 100 bases:

>100_bases
ACGGCCGACAGGAGAAACAAACATGAGCGACAAGAAAATCCGTTTGTCCGTAAAGGGCGTGAACAAGCGCTTCGGCGGTT
TGCAGGCATTGTCGGATGTC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTSIQPIEPSTTLIPERNLTKTLTVGIITAVFVIAAPMIIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYTAALLSSPHLSSQFEWIAHLAPAGLHVPFLIIIPCAMAIAAICGVILGAPTLRLRGDYLAIVTLGFGEIVRI
FLNNLDRPVNITNGPKGITGIDPIHVGDFSLAQTHSLFGLTFPSVYSYYYVFVLCALFVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRDVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTLPESIVVLACVVLGGMGHIPGVILGAVLLAVLP
EFLRSTMGPLQHMIFGHEIVDTEVIRQLVYALAMVLIMLYRSEGLWPSPKHEDKIAKLAKRNGKKPVRA

Sequences:

>Translated_389_residues
MTSIQPIEPSTTLIPERNLTKTLTVGIITAVFVIAAPMIIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYTAALLSSPHLSSQFEWIAHLAPAGLHVPFLIIIPCAMAIAAICGVILGAPTLRLRGDYLAIVTLGFGEIVRI
FLNNLDRPVNITNGPKGITGIDPIHVGDFSLAQTHSLFGLTFPSVYSYYYVFVLCALFVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRDVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTLPESIVVLACVVLGGMGHIPGVILGAVLLAVLP
EFLRSTMGPLQHMIFGHEIVDTEVIRQLVYALAMVLIMLYRSEGLWPSPKHEDKIAKLAKRNGKKPVRA
>Mature_388_residues
TSIQPIEPSTTLIPERNLTKTLTVGIITAVFVIAAPMIIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYIA
FYAVGAYTAALLSSPHLSSQFEWIAHLAPAGLHVPFLIIIPCAMAIAAICGVILGAPTLRLRGDYLAIVTLGFGEIVRIF
LNNLDRPVNITNGPKGITGIDPIHVGDFSLAQTHSLFGLTFPSVYSYYYVFVLCALFVIWVCTRLQHSRIGRAWAAIRED
EIAAKAMGINTRDVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTLPESIVVLACVVLGGMGHIPGVILGAVLLAVLPE
FLRSTMGPLQHMIFGHEIVDTEVIRQLVYALAMVLIMLYRSEGLWPSPKHEDKIAKLAKRNGKKPVRA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=346, Percent_Identity=42.1965317919075, Blast_Score=207, Evalue=7e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 41882; Mature: 41751

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TSIQPIEPSTTLIPERNLTKTLTVGIITAVFVIAAPMIIGAAGGNYWVRVLDFAMLYVM
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LALGLNVVVGFAGLLDLGYIAFYAVGAYTAALLSSPHLSSQFEWIAHLAPAGLHVPFLII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHH
IPCAMAIAAICGVILGAPTLRLRGDYLAIVTLGFGEIVRIFLNNLDRPVNITNGPKGITG
HHHHHHHHHHHHHHHCCCCEEECCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
IDPIHVGDFSLAQTHSLFGLTFPSVYSYYYVFVLCALFVIWVCTRLQHSRIGRAWAAIRE
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEIAAKAMGINTRDVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTLPESIVVLACVVL
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
GGMGHIPGVILGAVLLAVLPEFLRSTMGPLQHMIFGHEIVDTEVIRQLVYALAMVLIMLY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
RSEGLWPSPKHEDKIAKLAKRNGKKPVRA
HCCCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]