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The map label for this gene is oprM [H]

Identifier: 186475248

GI number: 186475248

Start: 546343

End: 547860

Strand: Direct

Name: oprM [H]

Synonym: Bphy_0480

Alternate gene names: 186475248

Gene position: 546343-547860 (Clockwise)

Preceding gene: 186475247

Following gene: 186475250

Centisome position: 15.7

GC content: 63.77

Gene sequence:

>1518_bases
ATGCATAAATATTCGTTGATGGCAGTGGCCGTCGCGCTGTTCGCCACGGGCTGCACGATGGAGCCGACGTACCACCAGCC
GGCCGCGCCCGTTTCGGGCACGTTCCCGAGCGACGGCGTGTACGCGACGCAACCCGTGCCCGGTGCGGGCGCGCGCTCGG
CGAACGGCCAGCCGGCGGTGGAGATCGGCTGGCGCGACTTCTTCGTCGATCAGCGCCTGCAACGTCTGATCGAGATCGCG
CTGAAGAACAATCGCGATCTGCGCGTGTCGGTGCTGAACATCGAGGCTTCGCGCGCGCAATATCAGATTGCGCGCGCGGC
GCTGTTCCCGGCGCTCGACGCTTCCGCGTCGCAGACGAAGCAGCGCACGCCGAAGAATCTGTCGGTGTTCGGCAATACGA
TTTCCAACGAGTATTCGGTCGGCTTGAATGCTTCGTGGGAAATCGACTTCTTCGGCCGGATTCGCAGCCTGAAGGACCAG
GCACTCGCGCAGTATCTGGCGACGGCACAGGCACGCAAGGCGGCGGAAATCGCGCTGGTCGCATCGGTCGCGGATCAGTA
CATGACCGTGCTGGCCTATGACGATCTGCTGAAGGTCACGGAGAACACGCTTAAGAGCGCGCAAGAGTCTTATCGGATCA
CGAAGTTGCAGTACGACACGGGTACGGGTTCTGAACTCGACCTGCGTCAGGCTCAGACCGTTGTCGAGCAGGCGACTGCG
AACCTGCAGTCGCAGGCGCGTTTGCGGGCGCAGGCTGAGAACGCACTTGTTCTCCTTGTTGGTGAGCCGTTGCCGTCTGA
TATGCCGAGCGGACTTATCCTCGACGATCAGAATCTGCTGACGGACATTCCGGCCGGTCTGCCGTCGGATCTGCTCACGC
GACGTCCCGACATCATGGAGGCCGAGCAGAACCTGCTGGCGGCCAACGCGAATATCGGTGCGGCGCGCGCGGCCTTCTTC
CCGAAGGTTTCGTTGACGGGCAGCTTCGGCACGCTGAGCCCGACACTCGGCGGGCTGTTCAAGCCAGGTTCGGCGGCGTG
GAGCTTCGCGCCGACGATCACGCTGCCGATCTTCGAAGGCGGCCAGAACATCGCGAACCTGGATCTCGCCAACGTCGAGA
AGAACATCCAGATCGCGCAGTACGAAAAGGCGATTCAGACGGCGTTTCGCGAGGTGGCCGACGGGCTCGCGGCGCGCGGC
ACCTACGATGAGCAGATCAAGGCGCTCGAACGCGATACGTTCGCGGAACAGCGTCGACTGGATCTGTCGGACCTGCGTTA
CCGGAATGGCGTCGACAGCTATCTCGCGGTGCTGACCGCGCAGACCAGCCTGTATAGCGCACAACAGCTGCTGATCACGG
CGCGCATGGAGCGTCTGACGAACCTCGTCGATCTGTACAAGGCGCTCGGCGGCGGCTGGATCGAACATGCAGGCGATCAG
CCGCGTCCGGCGGACGCGCCTGTCGATTACGGCGCGGCCAGCGCGCCTGTTGCCGCTTCGGCCGCGACGGCGGGCTGA

Upstream 100 bases:

>100_bases
AAGGAAGACCCGGACGAAGCGATGAAGCACTACAACGAGCACCATCCGCATGAACCGCCAGGCGGCAGCGGCCCGGGCAA
CGCCAGCTGAGGACATTGAG

Downstream 100 bases:

>100_bases
CCCACCGGGTTCGAGCGACGGAAGCTTCTTTTCCGACTGCAAAACGCCACGGCATGCCGTGGCGTTTTTTTTGCGCGCTT
CGTGCAAACGCTGGCCTGAT

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MHKYSLMAVAVALFATGCTMEPTYHQPAAPVSGTFPSDGVYATQPVPGAGARSANGQPAVEIGWRDFFVDQRLQRLIEIA
LKNNRDLRVSVLNIEASRAQYQIARAALFPALDASASQTKQRTPKNLSVFGNTISNEYSVGLNASWEIDFFGRIRSLKDQ
ALAQYLATAQARKAAEIALVASVADQYMTVLAYDDLLKVTENTLKSAQESYRITKLQYDTGTGSELDLRQAQTVVEQATA
NLQSQARLRAQAENALVLLVGEPLPSDMPSGLILDDQNLLTDIPAGLPSDLLTRRPDIMEAEQNLLAANANIGAARAAFF
PKVSLTGSFGTLSPTLGGLFKPGSAAWSFAPTITLPIFEGGQNIANLDLANVEKNIQIAQYEKAIQTAFREVADGLAARG
TYDEQIKALERDTFAEQRRLDLSDLRYRNGVDSYLAVLTAQTSLYSAQQLLITARMERLTNLVDLYKALGGGWIEHAGDQ
PRPADAPVDYGAASAPVAASAATAG

Sequences:

>Translated_505_residues
MHKYSLMAVAVALFATGCTMEPTYHQPAAPVSGTFPSDGVYATQPVPGAGARSANGQPAVEIGWRDFFVDQRLQRLIEIA
LKNNRDLRVSVLNIEASRAQYQIARAALFPALDASASQTKQRTPKNLSVFGNTISNEYSVGLNASWEIDFFGRIRSLKDQ
ALAQYLATAQARKAAEIALVASVADQYMTVLAYDDLLKVTENTLKSAQESYRITKLQYDTGTGSELDLRQAQTVVEQATA
NLQSQARLRAQAENALVLLVGEPLPSDMPSGLILDDQNLLTDIPAGLPSDLLTRRPDIMEAEQNLLAANANIGAARAAFF
PKVSLTGSFGTLSPTLGGLFKPGSAAWSFAPTITLPIFEGGQNIANLDLANVEKNIQIAQYEKAIQTAFREVADGLAARG
TYDEQIKALERDTFAEQRRLDLSDLRYRNGVDSYLAVLTAQTSLYSAQQLLITARMERLTNLVDLYKALGGGWIEHAGDQ
PRPADAPVDYGAASAPVAASAATAG
>Mature_505_residues
MHKYSLMAVAVALFATGCTMEPTYHQPAAPVSGTFPSDGVYATQPVPGAGARSANGQPAVEIGWRDFFVDQRLQRLIEIA
LKNNRDLRVSVLNIEASRAQYQIARAALFPALDASASQTKQRTPKNLSVFGNTISNEYSVGLNASWEIDFFGRIRSLKDQ
ALAQYLATAQARKAAEIALVASVADQYMTVLAYDDLLKVTENTLKSAQESYRITKLQYDTGTGSELDLRQAQTVVEQATA
NLQSQARLRAQAENALVLLVGEPLPSDMPSGLILDDQNLLTDIPAGLPSDLLTRRPDIMEAEQNLLAANANIGAARAAFF
PKVSLTGSFGTLSPTLGGLFKPGSAAWSFAPTITLPIFEGGQNIANLDLANVEKNIQIAQYEKAIQTAFREVADGLAARG
TYDEQIKALERDTFAEQRRLDLSDLRYRNGVDSYLAVLTAQTSLYSAQQLLITARMERLTNLVDLYKALGGGWIEHAGDQ
PRPADAPVDYGAASAPVAASAATAG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=475, Percent_Identity=41.2631578947368, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI2367348, Length=342, Percent_Identity=26.6081871345029, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54403; Mature: 54403

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKYSLMAVAVALFATGCTMEPTYHQPAAPVSGTFPSDGVYATQPVPGAGARSANGQPAV
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEE
EIGWRDFFVDQRLQRLIEIALKNNRDLRVSVLNIEASRAQYQIARAALFPALDASASQTK
EECHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHH
QRTPKNLSVFGNTISNEYSVGLNASWEIDFFGRIRSLKDQALAQYLATAQARKAAEIALV
HCCCCCEEEECCCCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASVADQYMTVLAYDDLLKVTENTLKSAQESYRITKLQYDTGTGSELDLRQAQTVVEQATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
NLQSQARLRAQAENALVLLVGEPLPSDMPSGLILDDQNLLTDIPAGLPSDLLTRRPDIME
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCHHHHHCCCCCCHHHHHCCCCHHH
AEQNLLAANANIGAARAAFFPKVSLTGSFGTLSPTLGGLFKPGSAAWSFAPTITLPIFEG
HHHHHEEECCCCCHHHHHHCCCEEECCCCCCCCHHHCCCCCCCCCCEEECCEEEEEEECC
GQNIANLDLANVEKNIQIAQYEKAIQTAFREVADGLAARGTYDEQIKALERDTFAEQRRL
CCCEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
DLSDLRYRNGVDSYLAVLTAQTSLYSAQQLLITARMERLTNLVDLYKALGGGWIEHAGDQ
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCC
PRPADAPVDYGAASAPVAASAATAG
CCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHKYSLMAVAVALFATGCTMEPTYHQPAAPVSGTFPSDGVYATQPVPGAGARSANGQPAV
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEE
EIGWRDFFVDQRLQRLIEIALKNNRDLRVSVLNIEASRAQYQIARAALFPALDASASQTK
EECHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHH
QRTPKNLSVFGNTISNEYSVGLNASWEIDFFGRIRSLKDQALAQYLATAQARKAAEIALV
HCCCCCEEEECCCCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASVADQYMTVLAYDDLLKVTENTLKSAQESYRITKLQYDTGTGSELDLRQAQTVVEQATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
NLQSQARLRAQAENALVLLVGEPLPSDMPSGLILDDQNLLTDIPAGLPSDLLTRRPDIME
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCHHHHHCCCCCCHHHHHCCCCHHH
AEQNLLAANANIGAARAAFFPKVSLTGSFGTLSPTLGGLFKPGSAAWSFAPTITLPIFEG
HHHHHEEECCCCCHHHHHHCCCEEECCCCCCCCHHHCCCCCCCCCCEEECCEEEEEEECC
GQNIANLDLANVEKNIQIAQYEKAIQTAFREVADGLAARGTYDEQIKALERDTFAEQRRL
CCCEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
DLSDLRYRNGVDSYLAVLTAQTSLYSAQQLLITARMERLTNLVDLYKALGGGWIEHAGDQ
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCC
PRPADAPVDYGAASAPVAASAATAG
CCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]