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The map label for this gene is mexA [H]

Identifier: 186475246

GI number: 186475246

Start: 541870

End: 543120

Strand: Direct

Name: mexA [H]

Synonym: Bphy_0478

Alternate gene names: 186475246

Gene position: 541870-543120 (Clockwise)

Preceding gene: 186475244

Following gene: 186475247

Centisome position: 15.57

GC content: 67.95

Gene sequence:

>1251_bases
ATGCGCGTCGAACGGGTTCCATTCCGCTTACTCACTGCCGCGACGGCTGCCGTAATGCTGGCAGCGTGCGGACAAAAACA
ATCAGCACCTCCGCCTCAAACTCCTGAAGTCGGCGTCGTCACGGTCCAGCCGCAAGCCGTGCCCGTCGTCACCGAACTGC
CGGGCCGCACCAGTGCGTTCCTCGTTGCGCAAGTGCGCGCGCGGGTGGACGGCATCGTGCTGCGCCGGGAATTTACGGAA
GGCACGCAGGTCAAGGCCGGCCAGCGTCTGTACAAGATCGACCCGGCGCCGTACATCGCCACGCTGAACAGCGCGAAGGC
ATCGCTTGCCAAGGCGCAGGCGAATCTCGCGTCGACCACCGCACAGGCGAACCGCTACAAGGTGCTGGTCGCGGCGAACG
CCGTCTCGAAGCAGGACTACGACAACGCCGTGGCCGCCGAAGGCCAGGCGGCCGCCGACGTCGCAGCGGGCAAGGCCGCC
GTCGACACCGCGCAGATCAACCTCGGCTATACGGACGTCGTGTCGCCCGTGTCGGGCCAGGTCGGGATATCGCAGGTCAC
GCCGGGCGCGTACGTGCAGGCGAGCCAGGCGACGCTGATGTCGACCGTGCAGCAACTCGATCCCGTCTACGTGGACGTCA
CGCAGTCGAGCCTTTCGGGCCTGAAGCTGCGCCGCGCAATCCAGGCAGGCCGCCTGAAGACCAACGGCCCGAACGCCGCG
AAGGTCGAGCTGGTGCTCGAAGACGGCCGCGTCTATTCGGAGAAGGGCAAGCTGCAGTTCACCGACGTCACCGTCGACCA
GACCACGGGCTCAGTGACGATCCGCGCGATCTTCGCGAACAAGGATCATGTGCTGCTGCCGGGCATGTTCGTGCGCGCGC
GCATTGAGGAAGGCGTCAACGAAAACGCGCTGCTGGTGCCGCAGATCGGCGTCACGCACGACCAGAAGGGCCAGCCGACG
GCGCTCGTACTGAACAAGGACGGCAAGGTCGAATTGCGCACGCTGCAGGCCACGTCGACCTACGGCCAGTACTGGGTGGT
CGAAGGCGGCCTGAATGTGGGCGATCGCGTGATCGTGAATGGCGTCGACAAGGTCAAACCGGGCGCGCAGGCGAAGGGCG
TGCCGGCCCAACTGCCGCCGCCGGCCGCAGCATCGGACGCGAGCGCGGCGGCTGCGCCGGGCGCGCCCGCCGCAAGCGGC
GCGCAGGCAGCCAGCGGCGCGGCCGCGGCCTCCGCTGCATCGGGCGCGTAA

Upstream 100 bases:

>100_bases
CGTCCGGTCACGGCGTCGTTGAGATGCCAATGTGCACAAGCCTTCTCCGGAGAGAGATGCACGCACTTTTTCCTTCTCAG
TCTTTGACAGAGGTCGCTCC

Downstream 100 bases:

>100_bases
TCAAGGGAGCCTGCTTCATGGCAAAGTTTTTTATTGATCGCCCGATCTTTGCTTGGGTGATCGCCATCATTCTGATGCTG
GCGGGTCTGGCGTCGATCTT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MRVERVPFRLLTAATAAVMLAACGQKQSAPPPQTPEVGVVTVQPQAVPVVTELPGRTSAFLVAQVRARVDGIVLRREFTE
GTQVKAGQRLYKIDPAPYIATLNSAKASLAKAQANLASTTAQANRYKVLVAANAVSKQDYDNAVAAEGQAAADVAAGKAA
VDTAQINLGYTDVVSPVSGQVGISQVTPGAYVQASQATLMSTVQQLDPVYVDVTQSSLSGLKLRRAIQAGRLKTNGPNAA
KVELVLEDGRVYSEKGKLQFTDVTVDQTTGSVTIRAIFANKDHVLLPGMFVRARIEEGVNENALLVPQIGVTHDQKGQPT
ALVLNKDGKVELRTLQATSTYGQYWVVEGGLNVGDRVIVNGVDKVKPGAQAKGVPAQLPPPAAASDASAAAAPGAPAASG
AQAASGAAAASAASGA

Sequences:

>Translated_416_residues
MRVERVPFRLLTAATAAVMLAACGQKQSAPPPQTPEVGVVTVQPQAVPVVTELPGRTSAFLVAQVRARVDGIVLRREFTE
GTQVKAGQRLYKIDPAPYIATLNSAKASLAKAQANLASTTAQANRYKVLVAANAVSKQDYDNAVAAEGQAAADVAAGKAA
VDTAQINLGYTDVVSPVSGQVGISQVTPGAYVQASQATLMSTVQQLDPVYVDVTQSSLSGLKLRRAIQAGRLKTNGPNAA
KVELVLEDGRVYSEKGKLQFTDVTVDQTTGSVTIRAIFANKDHVLLPGMFVRARIEEGVNENALLVPQIGVTHDQKGQPT
ALVLNKDGKVELRTLQATSTYGQYWVVEGGLNVGDRVIVNGVDKVKPGAQAKGVPAQLPPPAAASDASAAAAPGAPAASG
AQAASGAAAASAASGA
>Mature_416_residues
MRVERVPFRLLTAATAAVMLAACGQKQSAPPPQTPEVGVVTVQPQAVPVVTELPGRTSAFLVAQVRARVDGIVLRREFTE
GTQVKAGQRLYKIDPAPYIATLNSAKASLAKAQANLASTTAQANRYKVLVAANAVSKQDYDNAVAAEGQAAADVAAGKAA
VDTAQINLGYTDVVSPVSGQVGISQVTPGAYVQASQATLMSTVQQLDPVYVDVTQSSLSGLKLRRAIQAGRLKTNGPNAA
KVELVLEDGRVYSEKGKLQFTDVTVDQTTGSVTIRAIFANKDHVLLPGMFVRARIEEGVNENALLVPQIGVTHDQKGQPT
ALVLNKDGKVELRTLQATSTYGQYWVVEGGLNVGDRVIVNGVDKVKPGAQAKGVPAQLPPPAAASDASAAAAPGAPAASG
AQAASGAAAASAASGA

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=368, Percent_Identity=52.7173913043478, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI1789929, Length=357, Percent_Identity=49.0196078431373, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1789665, Length=367, Percent_Identity=48.7738419618529, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI87082036, Length=336, Percent_Identity=32.1428571428571, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42802; Mature: 42802

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVERVPFRLLTAATAAVMLAACGQKQSAPPPQTPEVGVVTVQPQAVPVVTELPGRTSAF
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCHHH
LVAQVRARVDGIVLRREFTEGTQVKAGQRLYKIDPAPYIATLNSAKASLAKAQANLASTT
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHH
AQANRYKVLVAANAVSKQDYDNAVAAEGQAAADVAAGKAAVDTAQINLGYTDVVSPVSGQ
CCCCEEEEEEEECCCCCCCCCCHHHCCCCCHHHHHCCCCEEEEEEEECCHHHHCCCCCCC
VGISQVTPGAYVQASQATLMSTVQQLDPVYVDVTQSSLSGLKLRRAIQAGRLKTNGPNAA
CCCEECCCCCEEEHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCE
KVELVLEDGRVYSEKGKLQFTDVTVDQTTGSVTIRAIFANKDHVLLPGMFVRARIEEGVN
EEEEEEECCEEEECCCCEEEEEEEEECCCCCEEEEEEEECCCCEEECCHHEEHHHHCCCC
ENALLVPQIGVTHDQKGQPTALVLNKDGKVELRTLQATSTYGQYWVVEGGLNVGDRVIVN
CCEEEEEECCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCEEEEEECCCCCCCEEEEC
GVDKVKPGAQAKGVPAQLPPPAAASDASAAAAPGAPAASGAQAASGAAAASAASGA
CCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure
MRVERVPFRLLTAATAAVMLAACGQKQSAPPPQTPEVGVVTVQPQAVPVVTELPGRTSAF
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCHHH
LVAQVRARVDGIVLRREFTEGTQVKAGQRLYKIDPAPYIATLNSAKASLAKAQANLASTT
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHH
AQANRYKVLVAANAVSKQDYDNAVAAEGQAAADVAAGKAAVDTAQINLGYTDVVSPVSGQ
CCCCEEEEEEEECCCCCCCCCCHHHCCCCCHHHHHCCCCEEEEEEEECCHHHHCCCCCCC
VGISQVTPGAYVQASQATLMSTVQQLDPVYVDVTQSSLSGLKLRRAIQAGRLKTNGPNAA
CCCEECCCCCEEEHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCE
KVELVLEDGRVYSEKGKLQFTDVTVDQTTGSVTIRAIFANKDHVLLPGMFVRARIEEGVN
EEEEEEECCEEEECCCCEEEEEEEEECCCCCEEEEEEEECCCCEEECCHHEEHHHHCCCC
ENALLVPQIGVTHDQKGQPTALVLNKDGKVELRTLQATSTYGQYWVVEGGLNVGDRVIVN
CCEEEEEECCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCEEEEEECCCCCCCEEEEC
GVDKVKPGAQAKGVPAQLPPPAAASDASAAAAPGAPAASGAQAASGAAAASAASGA
CCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]