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The map label for this gene is gloA [H]

Identifier: 186475170

GI number: 186475170

Start: 459311

End: 459697

Strand: Direct

Name: gloA [H]

Synonym: Bphy_0401

Alternate gene names: 186475170

Gene position: 459311-459697 (Clockwise)

Preceding gene: 186475169

Following gene: 186475183

Centisome position: 13.2

GC content: 54.01

Gene sequence:

>387_bases
ATGCGCCTTCTCCATACCATGCTCCGCGTGGGCAATCTCGATCGTTCGATCAAGTTCTACACCGAGCTGCTCGGAATGAA
AGTGCTGCGCCGCAATGACTATCCGGAAGGGAAGTTCACGCTCGCCTTCGTCGGTTATGAAGACGAGAAGGACGGTACGG
TGATCGAGCTGACGCACAACTGGGACACCGAGTCGTACGACATGGGCAATGCGTTTGGCCACCTCGCGGTGGAAGTCGAT
GATGCGTATGCCGCGTGTGAAAAGATCAAGCAGCAAGGCGGTAATGTTGTGCGTGAGGCTGGGCCTATGAAGCACGGTAC
GACCGTTATCGCGTTTGTTACTGACCCTGACGGGTACAAGATCGAGTTTATTCAGAAGAAGTCTTAA

Upstream 100 bases:

>100_bases
GGGGCTGCGTCATCTATTCTGAAGGTTGTCACTCAGCGAACGCGCGGCTTTTTCTGTACATTGACGTTCTCGACATCATC
AAGCAACAAGGGAGTGTACG

Downstream 100 bases:

>100_bases
ACCCAGCAAAAAAACCAAATGGCGACCGCGTCGCAGACAGAAAAAACCACTAAAGCGTAGGCAGGAGCTCAGGCGGATGA
TGCTTAAGCGTCTGCCGGGC

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MRLLHTMLRVGNLDRSIKFYTELLGMKVLRRNDYPEGKFTLAFVGYEDEKDGTVIELTHNWDTESYDMGNAFGHLAVEVD
DAYAACEKIKQQGGNVVREAGPMKHGTTVIAFVTDPDGYKIEFIQKKS

Sequences:

>Translated_128_residues
MRLLHTMLRVGNLDRSIKFYTELLGMKVLRRNDYPEGKFTLAFVGYEDEKDGTVIELTHNWDTESYDMGNAFGHLAVEVD
DAYAACEKIKQQGGNVVREAGPMKHGTTVIAFVTDPDGYKIEFIQKKS
>Mature_128_residues
MRLLHTMLRVGNLDRSIKFYTELLGMKVLRRNDYPEGKFTLAFVGYEDEKDGTVIELTHNWDTESYDMGNAFGHLAVEVD
DAYAACEKIKQQGGNVVREAGPMKHGTTVIAFVTDPDGYKIEFIQKKS

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=148, Percent_Identity=37.8378378378378, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787940, Length=127, Percent_Identity=66.1417322834646, Blast_Score=181, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=27.0676691729323, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=39.568345323741, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI20129743, Length=150, Percent_Identity=38.6666666666667, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19920404, Length=138, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14508; Mature: 14508

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMLRVGNLDRSIKFYTELLGMKVLRRNDYPEGKFTLAFVGYEDEKDGTVIELTHN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEEEEEEC
WDTESYDMGNAFGHLAVEVDDAYAACEKIKQQGGNVVREAGPMKHGTTVIAFVTDPDGYK
CCCCCCCCCCCCEEEEEEECHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEECCCCCE
IEFIQKKS
EEEEEECC
>Mature Secondary Structure
MRLLHTMLRVGNLDRSIKFYTELLGMKVLRRNDYPEGKFTLAFVGYEDEKDGTVIELTHN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEEEEEEC
WDTESYDMGNAFGHLAVEVDDAYAACEKIKQQGGNVVREAGPMKHGTTVIAFVTDPDGYK
CCCCCCCCCCCCEEEEEEECHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEECCCCCE
IEFIQKKS
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]