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The map label for this gene is ksgA

Identifier: 186475168

GI number: 186475168

Start: 457051

End: 457881

Strand: Direct

Name: ksgA

Synonym: Bphy_0399

Alternate gene names: 186475168

Gene position: 457051-457881 (Clockwise)

Preceding gene: 186475167

Following gene: 186475169

Centisome position: 13.14

GC content: 61.85

Gene sequence:

>831_bases
ATGTCCAACAGTTCAAGCAGACAGCACCAGGGCCACTTCGCGCGCAAGCGCTTCGGACAGAATTTTCTGGTCGACCTGGG
CATCATCGATTCAATTGTCGACGTGATCCGTCCACAGCGCGGCGAGCGCATGGTCGAAATTGGACCGGGGCTCGGTGCGC
TTACGGAACCGCTGGTCGAGCGTCTCGCGACGCCCGAAGCGCCGCTGCATGCTGTCGAACTCGACCGCGACCTGATCGGG
CGTCTGAAGAAGAAATTCGGCAACCTGCTCGAACTGCACGAAGGCGACGCGCTCGCGTTCGACTTCGGCTCGCTCGCGGG
CGAGGGCGACAAGCCGACGCTGCGTATCGTCGGCAATCTGCCGTACAACATTTCCAGCCCGCTGCTGTTTCATCTCGCCA
CCTTCGCCGAGCGCGTGATCGATCAGCATTTCATGTTACAGAACGAAGTGGTGGAGCGCATGGTCGCAGAGCCGGGCAGC
AAGGCGTTCAGCCGGTTGTCAGTGATGCTGCAGTATCGCTATGTGATCGACAAGCTGCTCGACGTGCCGCCCGAATCGTT
CCAGCCGCCGCCGAAAGTCGATTCCGCTATCGTGCGCATGATCCCGTACGCGCCGCACGAACTGCCCGCCGTCGACGAAA
TCAAGCTCGGCGAAGTCGTGACGGCGGCGTTTTCGCAGCGTCGCAAGATGTTGCGCAATACGTTGTCGACGTATCGCGAT
TCCGTCGATTTCGAAGCGCTCGGCTTCGATCTGCAACGCCGCGCCGAAGACGTGCCCGTCGCGGAATACGTGGCCGTTGC
GCAGGCCGTGTCGGCGGCAGCGGGCACTTAG

Upstream 100 bases:

>100_bases
CGGGCCGCGCGGATGCGGGCAGCCTGATCGCCGCGATCGACACCGCTGTTTTCATGGCGCGCAACCGGCGCGCCGCGTGA
TTTTTTCGTAGCTTCTTTCG

Downstream 100 bases:

>100_bases
TGCATCGTTGATGCATCAATCGCACCGCAACGTTTGATCGTTGCGTTCGCACAGATCAGTGTAGACAGCCCGCAACACGC
GGGCTTTCTTTTATCTTCCT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSNSSSRQHQGHFARKRFGQNFLVDLGIIDSIVDVIRPQRGERMVEIGPGLGALTEPLVERLATPEAPLHAVELDRDLIG
RLKKKFGNLLELHEGDALAFDFGSLAGEGDKPTLRIVGNLPYNISSPLLFHLATFAERVIDQHFMLQNEVVERMVAEPGS
KAFSRLSVMLQYRYVIDKLLDVPPESFQPPPKVDSAIVRMIPYAPHELPAVDEIKLGEVVTAAFSQRRKMLRNTLSTYRD
SVDFEALGFDLQRRAEDVPVAEYVAVAQAVSAAAGT

Sequences:

>Translated_276_residues
MSNSSSRQHQGHFARKRFGQNFLVDLGIIDSIVDVIRPQRGERMVEIGPGLGALTEPLVERLATPEAPLHAVELDRDLIG
RLKKKFGNLLELHEGDALAFDFGSLAGEGDKPTLRIVGNLPYNISSPLLFHLATFAERVIDQHFMLQNEVVERMVAEPGS
KAFSRLSVMLQYRYVIDKLLDVPPESFQPPPKVDSAIVRMIPYAPHELPAVDEIKLGEVVTAAFSQRRKMLRNTLSTYRD
SVDFEALGFDLQRRAEDVPVAEYVAVAQAVSAAAGT
>Mature_275_residues
SNSSSRQHQGHFARKRFGQNFLVDLGIIDSIVDVIRPQRGERMVEIGPGLGALTEPLVERLATPEAPLHAVELDRDLIGR
LKKKFGNLLELHEGDALAFDFGSLAGEGDKPTLRIVGNLPYNISSPLLFHLATFAERVIDQHFMLQNEVVERMVAEPGSK
AFSRLSVMLQYRYVIDKLLDVPPESFQPPPKVDSAIVRMIPYAPHELPAVDEIKLGEVVTAAFSQRRKMLRNTLSTYRDS
VDFEALGFDLQRRAEDVPVAEYVAVAQAVSAAAGT

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=217, Percent_Identity=33.1797235023041, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI156415992, Length=243, Percent_Identity=31.6872427983539, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786236, Length=272, Percent_Identity=50.7352941176471, Blast_Score=268, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI25146882, Length=221, Percent_Identity=32.579185520362, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25141369, Length=247, Percent_Identity=27.1255060728745, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324989, Length=220, Percent_Identity=27.7272727272727, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21358017, Length=261, Percent_Identity=30.2681992337165, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21357273, Length=265, Percent_Identity=30.188679245283, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_BURP8 (B2JCX3)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001856638.1
- ProteinModelPortal:   B2JCX3
- SMR:   B2JCX3
- GeneID:   6241901
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0399
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30615; Mature: 30483

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 21-21 BINDING 23-23 BINDING 48-48 BINDING 73-73 BINDING 96-96 BINDING 119-119

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSSSRQHQGHFARKRFGQNFLVDLGIIDSIVDVIRPQRGERMVEIGPGLGALTEPLVE
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
RLATPEAPLHAVELDRDLIGRLKKKFGNLLELHEGDALAFDFGSLAGEGDKPTLRIVGNL
HHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCCCEEEEEECC
PYNISSPLLFHLATFAERVIDQHFMLQNEVVERMVAEPGSKAFSRLSVMLQYRYVIDKLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
DVPPESFQPPPKVDSAIVRMIPYAPHELPAVDEIKLGEVVTAAFSQRRKMLRNTLSTYRD
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SVDFEALGFDLQRRAEDVPVAEYVAVAQAVSAAAGT
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNSSSRQHQGHFARKRFGQNFLVDLGIIDSIVDVIRPQRGERMVEIGPGLGALTEPLVE
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
RLATPEAPLHAVELDRDLIGRLKKKFGNLLELHEGDALAFDFGSLAGEGDKPTLRIVGNL
HHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCCCEEEEEECC
PYNISSPLLFHLATFAERVIDQHFMLQNEVVERMVAEPGSKAFSRLSVMLQYRYVIDKLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
DVPPESFQPPPKVDSAIVRMIPYAPHELPAVDEIKLGEVVTAAFSQRRKMLRNTLSTYRD
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SVDFEALGFDLQRRAEDVPVAEYVAVAQAVSAAAGT
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA