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The map label for this gene is ybhO [H]

Identifier: 186475152

GI number: 186475152

Start: 433879

End: 435159

Strand: Direct

Name: ybhO [H]

Synonym: Bphy_0383

Alternate gene names: 186475152

Gene position: 433879-435159 (Clockwise)

Preceding gene: 186475151

Following gene: 186475153

Centisome position: 12.47

GC content: 65.42

Gene sequence:

>1281_bases
ATGACGGGCGGCGGCCGCCGCCGCTTCGACCGGCTGACGCAGCATCTGCCCGGCCGGCGCTACTGGTCCAGGTATCGCTT
TTGTTCCGGCAACACGGTGCGGCTCTTCACGGCGGGCGAGCCATACTTTGCCGCGCTGACCCAACGCATCGACGCCGCAC
AACGTGAAGTCGCACTCGAAACCTACATCTTTTGCGACGACCCCGCGGGCCGCCCCGTTTCCGACGCGCTGATCCGCGCC
GCCAGCCGCGGTGTGCACGTACGGGTGATTACGGACGGCGTCGGCACCCAGCGTCTGCCGATGTTCAACGACTGGCAGGC
GGCGGGCATCGAGCATCGCATCTACAACCCGCACATCTTCGGACGCTTCGGCTTCTCGCGCACGCACCGCAAGCTTGCCG
TCGTCGATGGCGAGTTCGCCTACTGCGGCGGCATCAATGTCGTCGACGACTACGATCAGAACGGCGCGCGCCTGCCATAC
GCGCGCTGGGATTTCGCCGTCGAGTTGCAAGGGCCCGTCGTCGCCGACGTGCTCGAGGCGATCGACGTGCAGTGGGAGCG
CATCCGCATCGGCCATCGACCGGCGCTTGCGCCGCCCGCGGTCGGCGGATCGACGCCGCAGGCAGCCACCGCGCGTTTCG
TGCGCGCCCGAAGCAGCGCAGGTACGGCGGCTAGCGGACGAAGCTCGGACATGCGCGCGATCGCCGAGCCCTGCATCGCA
TTCGTCGCGCGCGACAACTTCGTCAACCGTCGTGCGATTGAAAGGGCATATCTGACCGCGATCGGCCAGGCGCGCTCCGA
AGTGCTGCTCGCCAATCCGTACTTCATGCCCGGGCGCAAGCTCCGGCGGGCGCTGATGCTGGCGGCGCAACGGGGCGTCG
ACGTGCGGCTCGTGATAGGCAGAAAGGAATTTGCCGTGCTGGATTACGCGGTGCCCTTCCTGTATCGCCAGTTGCTCAAG
TCCGGCGTGAAGATCGCCGAGTACGAGAAGACCATGCTGCATGGCAAGGTGGCCGTGGTCGATTCGAACTGGGGCACGGT
CGGCTCGTCCAATCTCGACGCGTTGAGCCTGATGCTGAACAATGAGGCCAATGTCGTGCTGGTGCTGCATCCGGAGATCG
ACCAGTTGCGTGACGCGATTCTGGCTGCCTTCGACGAGTCGCGCCGCATCGACGAAAAACACTACGAATCGCGTCCCGCG
GGCGAGCGTCTTCTGAACTGGGTCGCGTACAACACCTATCGGCTCGCGATGAAGCTGCTGACCGTCGGCGGCTACGATTA
A

Upstream 100 bases:

>100_bases
ATCTGTGGCTGCCTTGGCAAGAGGCTGCCGAGCGCTGCTTCTCGTGGTCGAATCGCGATGCGATCCTGCAATTGCCGCAG
CGCGTGAAGGAGCGTTGCCG

Downstream 100 bases:

>100_bases
GAAGATGACCGAATAAGGCAGACGAAATCCAGTCATAACAATCAAAATTGGGAGACAACCGTGCGCATTTCTGGCAAATG
ATTCTAAAAATTCCGCATCG

Product: phospholipase D/transphosphatidylase

Products: cardiolipin; glycerol [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTGGGRRRFDRLTQHLPGRRYWSRYRFCSGNTVRLFTAGEPYFAALTQRIDAAQREVALETYIFCDDPAGRPVSDALIRA
ASRGVHVRVITDGVGTQRLPMFNDWQAAGIEHRIYNPHIFGRFGFSRTHRKLAVVDGEFAYCGGINVVDDYDQNGARLPY
ARWDFAVELQGPVVADVLEAIDVQWERIRIGHRPALAPPAVGGSTPQAATARFVRARSSAGTAASGRSSDMRAIAEPCIA
FVARDNFVNRRAIERAYLTAIGQARSEVLLANPYFMPGRKLRRALMLAAQRGVDVRLVIGRKEFAVLDYAVPFLYRQLLK
SGVKIAEYEKTMLHGKVAVVDSNWGTVGSSNLDALSLMLNNEANVVLVLHPEIDQLRDAILAAFDESRRIDEKHYESRPA
GERLLNWVAYNTYRLAMKLLTVGGYD

Sequences:

>Translated_426_residues
MTGGGRRRFDRLTQHLPGRRYWSRYRFCSGNTVRLFTAGEPYFAALTQRIDAAQREVALETYIFCDDPAGRPVSDALIRA
ASRGVHVRVITDGVGTQRLPMFNDWQAAGIEHRIYNPHIFGRFGFSRTHRKLAVVDGEFAYCGGINVVDDYDQNGARLPY
ARWDFAVELQGPVVADVLEAIDVQWERIRIGHRPALAPPAVGGSTPQAATARFVRARSSAGTAASGRSSDMRAIAEPCIA
FVARDNFVNRRAIERAYLTAIGQARSEVLLANPYFMPGRKLRRALMLAAQRGVDVRLVIGRKEFAVLDYAVPFLYRQLLK
SGVKIAEYEKTMLHGKVAVVDSNWGTVGSSNLDALSLMLNNEANVVLVLHPEIDQLRDAILAAFDESRRIDEKHYESRPA
GERLLNWVAYNTYRLAMKLLTVGGYD
>Mature_425_residues
TGGGRRRFDRLTQHLPGRRYWSRYRFCSGNTVRLFTAGEPYFAALTQRIDAAQREVALETYIFCDDPAGRPVSDALIRAA
SRGVHVRVITDGVGTQRLPMFNDWQAAGIEHRIYNPHIFGRFGFSRTHRKLAVVDGEFAYCGGINVVDDYDQNGARLPYA
RWDFAVELQGPVVADVLEAIDVQWERIRIGHRPALAPPAVGGSTPQAATARFVRARSSAGTAASGRSSDMRAIAEPCIAF
VARDNFVNRRAIERAYLTAIGQARSEVLLANPYFMPGRKLRRALMLAAQRGVDVRLVIGRKEFAVLDYAVPFLYRQLLKS
GVKIAEYEKTMLHGKVAVVDSNWGTVGSSNLDALSLMLNNEANVVLVLHPEIDQLRDAILAAFDESRRIDEKHYESRPAG
ERLLNWVAYNTYRLAMKLLTVGGYD

Specific function: Catalyzes, in vitro, the phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787007, Length=384, Percent_Identity=36.9791666666667, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1787502, Length=392, Percent_Identity=29.0816326530612, Blast_Score=134, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 47590; Mature: 47459

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGGGRRRFDRLTQHLPGRRYWSRYRFCSGNTVRLFTAGEPYFAALTQRIDAAQREVALE
CCCCHHHHHHHHHHHCCCCHHHHHEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHEEEE
TYIFCDDPAGRPVSDALIRAASRGVHVRVITDGVGTQRLPMFNDWQAAGIEHRIYNPHIF
EEEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHCCCEEEECCCEEE
GRFGFSRTHRKLAVVDGEFAYCGGINVVDDYDQNGARLPYARWDFAVELQGPVVADVLEA
EECCCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCEEECCEEEEECCCHHHHHHHH
IDVQWERIRIGHRPALAPPAVGGSTPQAATARFVRARSSAGTAASGRSSDMRAIAEPCIA
HCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
FVARDNFVNRRAIERAYLTAIGQARSEVLLANPYFMPGRKLRRALMLAAQRGVDVRLVIG
HHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEC
RKEFAVLDYAVPFLYRQLLKSGVKIAEYEKTMLHGKVAVVDSNWGTVGSSNLDALSLMLN
CCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEEC
NEANVVLVLHPEIDQLRDAILAAFDESRRIDEKHYESRPAGERLLNWVAYNTYRLAMKLL
CCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
TVGGYD
HCCCCC
>Mature Secondary Structure 
TGGGRRRFDRLTQHLPGRRYWSRYRFCSGNTVRLFTAGEPYFAALTQRIDAAQREVALE
CCCHHHHHHHHHHHCCCCHHHHHEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHEEEE
TYIFCDDPAGRPVSDALIRAASRGVHVRVITDGVGTQRLPMFNDWQAAGIEHRIYNPHIF
EEEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHCCCEEEECCCEEE
GRFGFSRTHRKLAVVDGEFAYCGGINVVDDYDQNGARLPYARWDFAVELQGPVVADVLEA
EECCCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCEEECCEEEEECCCHHHHHHHH
IDVQWERIRIGHRPALAPPAVGGSTPQAATARFVRARSSAGTAASGRSSDMRAIAEPCIA
HCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
FVARDNFVNRRAIERAYLTAIGQARSEVLLANPYFMPGRKLRRALMLAAQRGVDVRLVIG
HHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEC
RKEFAVLDYAVPFLYRQLLKSGVKIAEYEKTMLHGKVAVVDSNWGTVGSSNLDALSLMLN
CCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEEC
NEANVVLVLHPEIDQLRDAILAAFDESRRIDEKHYESRPAGERLLNWVAYNTYRLAMKLL
CCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
TVGGYD
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 L-1-phosphatidyl-glycerol [C]

Specific reaction: 2 L-1-phosphatidyl-glycerol = cardiolipin + glycerol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]