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The map label for this gene is phbI [H]

Identifier: 186475031

GI number: 186475031

Start: 305778

End: 307520

Strand: Direct

Name: phbI [H]

Synonym: Bphy_0262

Alternate gene names: 186475031

Gene position: 305778-307520 (Clockwise)

Preceding gene: 186475030

Following gene: 186475034

Centisome position: 8.79

GC content: 64.43

Gene sequence:

>1743_bases
GTGTCCTTCACGCTGCATGGAATTCCCGTGTCACGCGGTATCGCCATCGGGCGGGCATATCTGATCGCCCCGGCTGCACT
GGACGTCGACCACTATCTGATCGAGCCCTCGCAGATCGAGGGCGAGGTGGAGCGTTTTCGTGCGGCGCAGGCAGTCGTGC
ATCGGGAACTCGCCGAACTCCGCGCCGATCTCGCCGCGGACGCGCCCTCTGAAATGGGCGCCTTCATCGACGTGCATGCC
ATGATCCTGAACGACGCGATGCTCGTTCAGGAAACCATCGACCTCATCCGTACGCGCCGCTACAACGTGGAGTGGGCGCT
GACCGAGCAGCTCGAACGTCTCACGCGCCACTTCGACGAAGTAGAAGACGAGTATCTGCGCGAGCGCAAGGCCGACATCG
AACAGGTGGTCGAGCGCGTGCTGAAGGCGCTCGCGGGCGCGACGGGTGCGCTCGTCAATGGCATTCATGGGCGCTGCGAC
GAAATGATCGTCGTCGCGCACGACATCGCGCCCGCCGACATGATGCAGTTCAAGACGCAGACGTTCCAGGGCTTCGTGAC
GGATCTGGGCGGCCGTACCTCGCATACGGCGATCGTCGCGCGCAGTCTCGGCATTCCCGCGGCGGTCGGCGTGCAGCACG
CAAGCGCACTGATCCGGCAGGATGATCTGATCATTGTCGACGGCGATCACGGCATCGTGATCGTCGATCCCGCGCCGATC
GTGCTCGAAGAGTACTCCTACCGGCAGAGCGAAAAGGCGCTGGAAGCGCGCAAGCTGCAGCGCCTGAAGTTCTCGCCGAC
ACAGACGGTGTGCGGCACGCGTATCGAGCTGTGCGCGAACATCGAATTGCCCGACGACGCGCAGGCCGCGCTCGACGCGG
GCGCGACAGGGATCGGCCTGTTCCGCACCGAGTTTCTGTTCATGAACACGAAGGACCGGATGCCGGAGGAGGAAGAGCAG
TTCGCCGCATACCGCCGCGCCGTCGAGTTGATGAACGGCATGCCCGTGACGATCCGCACGATCGACGTCGGCGCGGATAA
GCCGCTCGAGTCGATCGGCGACGAGTTCGAGACGGCGCCCAACCCGGCGCTCGGTCTGCGCGCGATCCGTTGGAGCCTGT
CCGAGCCGCACATGTTTCTCACGCAACTGCGCGCGATTCTCCGCGCATCGGCGTTCGGTTCGGTGAAGATCCTGATTCCG
ATGCTTGCGCACGCGCGCGAGATCGATCAGACGCTCGACCTCATTCAGGAAGCCAAGCGCCAGCTCGATGACGCGGGCCT
CGCGTACGATCCGAACGTGCGCGTCGGCGCGATGATCGAGATTCCGGCGGCGGCGATCGCGTTGCCGCTGTTCCTGAAGC
GGCTCGATTTCCTCTCGATCGGCACCAATGACCTGATCCAGTACACGCTCGCGATCGATCGCGCGGACAACGCAGTGGCG
CATCTGTACGACCCGCTGCATCCCGCCGTTCTGCATTTGATCGCGTTTACGCTTCGCGAGGCGAAGCGTGCGGGTGTGCC
GGTGTCGATCTGCGGAGAAATGGCGGGCGATCCGTCGCTCACGCGCCTGCTGCTCGGCATGGGACTGACGGAGTTCTCGA
TGCATCCGAGCCAGTTGCTGCTCGTGAAGCAGGAGGTGCTGCGCGCTCAGCTGAAGGCGCTGGAAAAGCCGGTTGCCGAC
GTGCTGGCCGCGTTCGAACCGGAAGAGGTGCAGTCAGCGCTGGAGCGGGTCGCACAGGCCTGA

Upstream 100 bases:

>100_bases
CTGCACAGCACGGTTAACGGGACGGAATTTATAATGACCGTCGGGGTCTGAGAGCACTGCAGCGGTTTGCCGCGGCATCA
CACAACTAGAGGAGGTGCGC

Downstream 100 bases:

>100_bases
CGTTCATGCCGACAGGCGCGCGGGGCGCGGTCGGCAGGCAATTTACTTTACGAACGATGCCGCTCGCCGCACACCGGGCA
ATCCGGCTGGCGCGCGATGC

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MSFTLHGIPVSRGIAIGRAYLIAPAALDVDHYLIEPSQIEGEVERFRAAQAVVHRELAELRADLAADAPSEMGAFIDVHA
MILNDAMLVQETIDLIRTRRYNVEWALTEQLERLTRHFDEVEDEYLRERKADIEQVVERVLKALAGATGALVNGIHGRCD
EMIVVAHDIAPADMMQFKTQTFQGFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDHGIVIVDPAPI
VLEEYSYRQSEKALEARKLQRLKFSPTQTVCGTRIELCANIELPDDAQAALDAGATGIGLFRTEFLFMNTKDRMPEEEEQ
FAAYRRAVELMNGMPVTIRTIDVGADKPLESIGDEFETAPNPALGLRAIRWSLSEPHMFLTQLRAILRASAFGSVKILIP
MLAHAREIDQTLDLIQEAKRQLDDAGLAYDPNVRVGAMIEIPAAAIALPLFLKRLDFLSIGTNDLIQYTLAIDRADNAVA
HLYDPLHPAVLHLIAFTLREAKRAGVPVSICGEMAGDPSLTRLLLGMGLTEFSMHPSQLLLVKQEVLRAQLKALEKPVAD
VLAAFEPEEVQSALERVAQA

Sequences:

>Translated_580_residues
MSFTLHGIPVSRGIAIGRAYLIAPAALDVDHYLIEPSQIEGEVERFRAAQAVVHRELAELRADLAADAPSEMGAFIDVHA
MILNDAMLVQETIDLIRTRRYNVEWALTEQLERLTRHFDEVEDEYLRERKADIEQVVERVLKALAGATGALVNGIHGRCD
EMIVVAHDIAPADMMQFKTQTFQGFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDHGIVIVDPAPI
VLEEYSYRQSEKALEARKLQRLKFSPTQTVCGTRIELCANIELPDDAQAALDAGATGIGLFRTEFLFMNTKDRMPEEEEQ
FAAYRRAVELMNGMPVTIRTIDVGADKPLESIGDEFETAPNPALGLRAIRWSLSEPHMFLTQLRAILRASAFGSVKILIP
MLAHAREIDQTLDLIQEAKRQLDDAGLAYDPNVRVGAMIEIPAAAIALPLFLKRLDFLSIGTNDLIQYTLAIDRADNAVA
HLYDPLHPAVLHLIAFTLREAKRAGVPVSICGEMAGDPSLTRLLLGMGLTEFSMHPSQLLLVKQEVLRAQLKALEKPVAD
VLAAFEPEEVQSALERVAQA
>Mature_579_residues
SFTLHGIPVSRGIAIGRAYLIAPAALDVDHYLIEPSQIEGEVERFRAAQAVVHRELAELRADLAADAPSEMGAFIDVHAM
ILNDAMLVQETIDLIRTRRYNVEWALTEQLERLTRHFDEVEDEYLRERKADIEQVVERVLKALAGATGALVNGIHGRCDE
MIVVAHDIAPADMMQFKTQTFQGFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDHGIVIVDPAPIV
LEEYSYRQSEKALEARKLQRLKFSPTQTVCGTRIELCANIELPDDAQAALDAGATGIGLFRTEFLFMNTKDRMPEEEEQF
AAYRRAVELMNGMPVTIRTIDVGADKPLESIGDEFETAPNPALGLRAIRWSLSEPHMFLTQLRAILRASAFGSVKILIPM
LAHAREIDQTLDLIQEAKRQLDDAGLAYDPNVRVGAMIEIPAAAIALPLFLKRLDFLSIGTNDLIQYTLAIDRADNAVAH
LYDPLHPAVLHLIAFTLREAKRAGVPVSICGEMAGDPSLTRLLLGMGLTEFSMHPSQLLLVKQEVLRAQLKALEKPVADV
LAAFEPEEVQSALERVAQA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=566, Percent_Identity=38.86925795053, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1789193, Length=537, Percent_Identity=35.3817504655494, Blast_Score=305, Evalue=6e-84,
Organism=Escherichia coli, GI48994992, Length=475, Percent_Identity=33.8947368421053, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1788726, Length=577, Percent_Identity=28.42287694974, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1787994, Length=397, Percent_Identity=27.2040302267003, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI226510935, Length=167, Percent_Identity=26.3473053892216, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63849; Mature: 63718

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTLHGIPVSRGIAIGRAYLIAPAALDVDHYLIEPSQIEGEVERFRAAQAVVHRELAEL
CCEEEECCCHHCCHHHHHHHEEECHHCCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHH
RADLAADAPSEMGAFIDVHAMILNDAMLVQETIDLIRTRRYNVEWALTEQLERLTRHFDE
HHHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VEDEYLRERKADIEQVVERVLKALAGATGALVNGIHGRCDEMIVVAHDIAPADMMQFKTQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHH
TFQGFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDHGIVIVDPAPI
HHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCH
VLEEYSYRQSEKALEARKLQRLKFSPTQTVCGTRIELCANIELPDDAQAALDAGATGIGL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEECCCCCCCHHHHHHCCCCCHHH
FRTEFLFMNTKDRMPEEEEQFAAYRRAVELMNGMPVTIRTIDVGADKPLESIGDEFETAP
HHHHEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHCHHHHCCC
NPALGLRAIRWSLSEPHMFLTQLRAILRASAFGSVKILIPMLAHAREIDQTLDLIQEAKR
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLDDAGLAYDPNVRVGAMIEIPAAAIALPLFLKRLDFLSIGTNDLIQYTLAIDRADNAVA
HHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEHHEEECCCCHHH
HLYDPLHPAVLHLIAFTLREAKRAGVPVSICGEMAGDPSLTRLLLGMGLTEFSMHPSQLL
HHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCCHHHCCCHHHHH
LVKQEVLRAQLKALEKPVADVLAAFEPEEVQSALERVAQA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SFTLHGIPVSRGIAIGRAYLIAPAALDVDHYLIEPSQIEGEVERFRAAQAVVHRELAEL
CEEEECCCHHCCHHHHHHHEEECHHCCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHH
RADLAADAPSEMGAFIDVHAMILNDAMLVQETIDLIRTRRYNVEWALTEQLERLTRHFDE
HHHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VEDEYLRERKADIEQVVERVLKALAGATGALVNGIHGRCDEMIVVAHDIAPADMMQFKTQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHH
TFQGFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDHGIVIVDPAPI
HHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCH
VLEEYSYRQSEKALEARKLQRLKFSPTQTVCGTRIELCANIELPDDAQAALDAGATGIGL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEECCCCCCCHHHHHHCCCCCHHH
FRTEFLFMNTKDRMPEEEEQFAAYRRAVELMNGMPVTIRTIDVGADKPLESIGDEFETAP
HHHHEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHCHHHHCCC
NPALGLRAIRWSLSEPHMFLTQLRAILRASAFGSVKILIPMLAHAREIDQTLDLIQEAKR
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLDDAGLAYDPNVRVGAMIEIPAAAIALPLFLKRLDFLSIGTNDLIQYTLAIDRADNAVA
HHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEHHEEECCCCHHH
HLYDPLHPAVLHLIAFTLREAKRAGVPVSICGEMAGDPSLTRLLLGMGLTEFSMHPSQLL
HHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCCHHHCCCHHHHH
LVKQEVLRAQLKALEKPVADVLAAFEPEEVQSALERVAQA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]